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Mitochondrial tRNA^(leu(UUR)) gene mutation diabetes mellitus in Chinese

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Chinese Medical Journal 1997年第5期 52-58页

作者：项坤三王延庆吴松华陆惠娟郑泰山孙多奇翁青贾伟平沈卫平浦鎏何进卫

Objective To ascertain the prevalence and clinical features of mitochondrial tRNA leu(UUR) gene nucleotide (nt) 3243 A→G mutation diabetes mellitus in Chinese and to establish the approaches for genetic diagnosis of this subtype of diabetes during routine daily clinical practice. Methods Mitochondrial nt 3243 A→G mutation was screened among 207 unrelated non insulin dependent diabetes mellitus (NIDDM) patients by using polymerase chain reaction (PCR)/Apa Ⅰ restriction endonuclease digestion. Samples with positive result were confirmed by DNA sequence analysis. Genetic and clinical analysis were carried out in family members of the proband with positive genetic diagnosis. Results Positive results were found in two subjects during screening of unrelated NIDDM patients, which account for 2.4% 11.1% of NIDDM subjects with uncommon clinical features (with early age of onset, with low body mass index and on insulin therapy) or with positive family history of diabetes consistent with or unable to exclude from maternal inheritance. Genetic diagnosis was positive in 10 out of 25 famliy members in the pedigrees of these two probands. Conclusions Mitochondrial tRNA leu(UUR) gene nt 3242 A→G mutation should be considered in diabetic patients with the above mentioned clinical features. The genetic diagnosis in daily clinical practice can be performed with allele specific priming amplification, or PCR/Hae Ⅲ or Apa Ⅰ digestion which are technically simple to perform and diagnostically easy to define.

关键词：项坤三 leu UUR Mitochondrial tRNA gene mutation mellitus diabetes 吴松 Chinese

leucine regulates lipid metabolism in adipose tissue through adipokine-mTOR-SIRT1 signaling pathway and bile acid—microbe axis in a finishing pig model

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Animal Nutrition 2024年第1期16卷 158-173页

作者： Yunju Yin Saiming Gong Mengmeng Han Jingzun Wang Hanjing Shi Xianji Jiang Liu Guo Yehui Duan Qiuping Guo Qinghua Chen Fengna Li Key Laboratory of Agro-ecological Processes in Subtropical Region Institute of Subtropical AgricultureChinese Academy of SciencesHunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic ProcessHunan Provincial Engineering Research Center for Healthy Livestock and Poultry ProductionChangsha 410125China College of Animal Science and Technology Hunan Agricultural UniversityChangsha 410128China College of Modern Agricultural Sciences University of Chinese Academy of SciencesBeijing 100049China College of Life Science and Engineering Northwest Minzu UniversityLanzhou 730124China College of Life Sciences Hunan Normal UniversityChangsha 410128China

This study was conducted to explore the regulatory mechanism of leucine(leu)on lipid metabolism of finishing ***-four Duroc×Landrace×Large cross pigs with an average body weight of68.33±0.97 kg were randomly allocated into 3 treatment groups with 8 replicates per group(1 pig per replicate).The dietary treatments were as follows:control group(CON),0.25%leu group and 0.50%leu *** experimental period was 42 *** results showed as follows.(1)Compared with the CON,0.25%and 0.50%leu increased(Pleu group,the relative mRNA expression levels of sterol regulatory element binding protein-1c(SREBP1c),recombinant fatty acid transport protein 1(FATP1),chemerin and peroxisome proliferatoractivated receptorγ(PPARγ)were decreased but the level of fatty acid binding protein 4(FABP4)and fatty acid translocase(FAT/CD36)were increased in backfat *** the 0.25%leu group,the protein levels of p-Rictor,p-Raptor,p-eIF4E-binding protein 1(p-4EBP1),p-silent mating type information regulator 2homolog 1(p-SIRT1)and acetylation ribosome s6 protein kinase 1(Ac—S6K1)were increased(Pleu group was *** component analysis showed that the relative abundance of Bacteroidetes,Lactobacillus and Desulfovibrio was higher in the 0.25%leu group than the CON,but the relative abundance of Firmicutes,Treponema and Shigella was lower than in the CON(Pleu could promote adipose tissue lipolysis of finishing pigs through the mTOR-SIRT1 signaling pathway,a

关键词： leu Lipid metabolism Gut microbiota Bile acid

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MCNP Simulation of Physics Parameters of Dispersion Fuels for Conversion of NIRR-1 to leu

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World Journal of Nuclear Science and Technology 2018年第2期8卷 23-29页

作者： Kola Ibikunle Umar Sadiq Yakubu Viva Ibrahim Sunday Adesunloye Jonah Department of Physics Ahmadu Bello University Zaria Nigeria Centre for Energy Research and Training Ahmadu Bello University Zaria Nigeria

The Nigeria Research Reactor-1 (NIRR-1) is one of the Commercial Miniature Neutron Source Reactors (MNSRs) sited outside China and scheduled for conversion under the auspices of Reduced Enrichment for Research and Test Reactors (RERTR) program. Since 2006, the reduction in the fuel enrichment of MSNR facilities from greater than 90% HEU cores to less than 20% leu cores has been embarked upon. Consequently in this work, the physics parameters of three dispersion leu fuels, which include U3Si, U3Si2, and U9Mo enriched to 19.75% were determined by the MCNP code to investigate their suitability for the conversion of NIRR-1 to leu. The following reactor core physics parameters were computed for the leu fuel options: clean cold core excess reactivity (ρex), control rod (CR) worth, shut down margin (SDM), neutron flux distributions in the irradiation channels and kinetics data (i.e. effective delayed neutron fraction, βeff?and prompt neutron lifetime, lf). Results are compared with experimental and calculated data of the current HEU core and indicate that it would be feasible to use any of the leu options for the conversion of commercial MNSR in general and NIRR-1 in particular from HEU to leu.

关键词： Neutronics Data HEU to leu Conversion MNS Reactors Dispersion Fuels MCNP

Overproduction and purification of Escherichia colitRNA^(leu)

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Science China(Life Sciences) 1998年第3期41卷 225-231页

作者：李勇王恩多王应睐 Statc Key Labratory of Molecular Biology Shanghai Institute of Biochemistry Chinese Academy of Sciences Shanghai China

Chemically synthesized genes encoding \%Escherichia coli\% tRNA leu 1 and tRNA leu 2 were ligated into the plasmid pTrc99B, then transformed into \%Escherichia coli\% MT102, respectively. The positive transformants, named MT leu1 and MT leu2, were confirmed by DNA sequencing, and the conditions of cultivation for the two transformants were optimized. As a result, leucine accepting activity of their total tRNA reached 810 and 560 pmol/A 260 , respectively: the content of tRNA leu 1 was 50% of total tRNA from MT leu1, while that of tRNA leu 2 was 30% of total tRNA from MT leu2. Both tRNA leu s from their total tRNAs were fractionated to 1 600 pmol/A 260 after DEAE Sepharose and BD cellulose column chromatography. The accurate kinetic constants of aminoacylation of the two isoacceptors of tRNA leu catalyzed by leucyl tRNA synthetase were determined.

关键词： tRNA leu 1 tRNA leu 2 cloning high expression purification.

广西壮族人群CYP1B1基因leu 432 Va1多态性分析

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中国公共卫生 2012年第7期28卷 936-938页

作者：廖长秀李曙波唐卫东何昀黎为能右江民族医学院药理教研室广西百色533000 右江民族医学院肿瘤分子实验室右江民族医学院生物化学教研室

目的研究CYP1B1第432密码子在广西壮族人群中遗传特征,为进一步研究CYP1B1基因多态性与疾病的发生易感性奠定基础。方法采用人工修饰双等位基因特异性引物扩增法(diASA-AMP)技术对288名广西壮族正常成人进行CYP1B1 leu 432 Va1基因分型... 详细信息

目的研究CYP1B1第432密码子在广西壮族人群中遗传特征,为进一步研究CYP1B1基因多态性与疾病的发生易感性奠定基础。方法采用人工修饰双等位基因特异性引物扩增法(diASA-AMP)技术对288名广西壮族正常成人进行CYP1B1 leu 432 Va1基因分型,探讨性别间差异及与国内外其他人群分布频率差异。结果广西壮族女性和男性人群CYP1B1 leu 432 Va1均以C等位基因为主,分别为89.1%和91.4%;CC野生纯合子型、CG杂合子型和GG突变纯合型在女性人群中分别为78.3%、21.3%和0,男性人群则为83.6%、15.6%和0.8%;统计分析表明,广西壮族CYP1B1 leu 432 Va1等位基因和基因型分布频率无性别差异,与朝鲜人群相应位点基因型分布频率无差异,但与国内江苏汉族、上海和四川女性相比具有统计学差异(Pleu 432 Va1呈多态性分布,基因型分布频率无性别差异,存在种族差异。

关键词： CYP1B1 leu 432 Va1 基因多态性广西壮族

CTCS点式应答器系统-leu与可变信息应答器的研究

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CTCS点式应答器系统-LEU与可变信息应答器的研究

作者：于磊电子科技大学

学位级别：硕士

CTCS点式应答器系统是中国自己开发的列车控制系统,本论文所研究的课题是CTCS的地面子系统。目标是设计符合要求的道旁电子单元(leu)与可变信息应答器。leu将列车控制中心发送的控车指令处理后经过可变信息应答器及时地传给车载设备,实... 详细信息

CTCS点式应答器系统是中国自己开发的列车控制系统,本论文所研究的课题是CTCS的地面子系统。目标是设计符合要求的道旁电子单元(leu)与可变信息应答器。leu将列车控制中心发送的控车指令处理后经过可变信息应答器及时地传给车载设备,实现对高速列车的自动控制。本文在对CTCS列控系统功能及原理理解的基础上,设计了符合我国铁路现状的leu和可变信息应答器。解决了以下关键技术:1、采用独立的接收模块,实现了列控中心点灯接口与RS-485接口的兼容;2、采用CPLD开发设计编码模块,提取报文并编成曼彻斯特码,完成远距离发送;3、设计了记录模块,实现了对leu工作过程的监控;4、设计了可变信息应答器的接口板,解决了可变信息应答器的输入信号与调制模块的衔接问题;5、完成了相关软件的设计;本文采用了接收模块,编码模块,记录模块组合的方式设计了leu,它具有564.48kbps高速传输和不小于3km远距离发送报文的功能,并且能同时控制四路不同的可变信息应答器。

关键词： leu 可变信息应答器报文曼彻斯特码 CPLD

RELATIONSHIP BETWEEN ACUPUNGTURE ANALGESIA AND PLASMA leu-ENKE-PHALIN, PLASMA CORTISOL, TYPE DIFFE-RENTIATION IN CHINESE MEDICME

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针刺研究 1989年第Z1期 158-158页

作者： He Lianfang Liu Zhongying Lu Yanyan Wang Miaozhen Institute of Acupuncture Research, Shanghai Medical University, Shanghai, China

An investigation was undertaken on chronic pain patients treated with acu-puncture and differentiated into two groups, one with yang deficiency and theother without yang deficiency. The efficacy of acupuncture therapy was eva-luated by the visual analogue scale. The contents of plasma leu-enkephalinlike immunoreactive substance (LEKLIS) and plasma cortisol were measured byradioimmunoassay. It was found that the therapeutic effect of acupuncture wasassociated with changes of plasma LEKLIS before and after acupuncture. Mostpatients with yang deficiency manifested good effect and their plasma LEKLIS

关键词： leu TYPE DIFFE-RENTIATION IN CHINESE MEDICME RELATIONSHIP BETWEEN ACUPUNGTURE ANALGESIA AND PLASMA leu-ENKE-PHALIN PLASMA CORTISOL

酵母脯氨酸合成酶基因（Pro2）的分离及leu+ Pro+表型共转导

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Journal of Genetics and Genomics 1986年第3期 163-171页

作者：宣建武,匡达人中国科学院上海细胞生物学研究所中国科学院上海细胞生物学研究所安徽合肥中国科技大学生物系

酵母7209-1 A（a）DNA,用pHC79为载体,以BamHⅠ、PstⅠ和Hind Ⅲ酶切,经体外重组和包装后,转导至大肠杆菌HB101和SC294中。在我们的实验条件下（对酵母DNA适度酶解,F/V值为15,连接时DNA总浓度为200—250微克/微升以及BHB 2688/BHB2690... 详细信息

酵母7209-1 A（a）DNA,用pHC79为载体,以BamHⅠ、PstⅠ和Hind Ⅲ酶切,经体外重组和包装后,转导至大肠杆菌HB101和SC294中。在我们的实验条件下（对酵母DNA适度酶解,F/V值为15,连接时DNA总浓度为200—250微克/微升以及BHB 2688/BHB2690比值为5）,重组建库效率达到10～6CFU/微克载体DNA,重组子双抗值降低到10％或5％以下。大肠杆菌HB101和SC 294的10～6个重组体克隆中,观察到酵母基因对leuB 6及proA2（在HB101中）,leu6,metB1,argG,his1（在SC294中）等营养缺陷型的互补（校正）效应。由于互补频率(10-3—10-4)比上述基因突变自发回复率高2—3个数量级,因此可以说,酵母的上述基因在大肠杆菌中获得了功能表达。对pYeleu5和pYeleu7单克隆DNA的再次包装转导和再次互补测试表明,我们已使酵母leu+的互补效率提高10～4倍。本文还报道了在HB101中的酵母基因文库leu+Pro+的表型共转导现象,频率为30％以上。

关键词：酵母 HB leu Pro2 共转导体外包装酵母基因 DNA 表型

电沉积leu UO_2靶件生产医用^(99)Mo的工艺研究

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同位素 2018年第3期31卷 165-172页

作者：梁积新沈亦佳吴宇轩向学琴于宁文郭澍吴久伟罗志福中国原子能科学研究院同位素研究所北京102413

^(99)Mo是一种重要的医用放射性同位素。采用低浓铀(leu)靶件生产裂变^(99)Mo是发展趋势。本工作进行了电沉积UO_2靶件制备、靶件溶解以及99Mo化学分离等工艺研究,确定了电沉积leu UO_2靶件制备医用裂变99Mo的工艺流程。研究表明,于不... 详细信息

^(99)Mo是一种重要的医用放射性同位素。采用低浓铀(leu)靶件生产裂变^(99)Mo是发展趋势。本工作进行了电沉积UO_2靶件制备、靶件溶解以及99Mo化学分离等工艺研究,确定了电沉积leu UO_2靶件制备医用裂变99Mo的工艺流程。研究表明,于不锈钢管内壁上电沉积UO_2,在p H=7、电流0.5~2 m A/cm2、温度75~90℃、镀液中U浓度5 mg/mL条件下,经过约210 h电沉积,不锈钢管内壁上UO_2沉积层质量达到42 mg/cm^2;采用6 mol/L HNO_3溶解UO_2镀层。采用α-安息香肟沉淀法实现^(99)Mo与大量裂变产物的初步分离,采用阴离子交换法与活性炭色层法联用实现99Mo的纯化;纯化后的99Mo溶液中,杂质^(131)I、^(90)Sr、^(95)Zr、^(103)Ru、^(238)U活度与^(99)Mo活度比值分别为4.47×10^(-6)%、7.40×10^(-7)%、8.67×10^(-7)%、2.57×10^(-6)%、1.69×10^(-14)%,均小于《欧洲药典》规定值,满足医用要求。本工作建立了电沉积leu UO_2靶件生产高纯医用裂变99Mo的工艺流程,为今后采用leu技术生产医用裂变99Mo,进而实现其自主规模化生产打下了基础。

关键词：裂变99Mo leu 电沉积UO2靶件工艺流程