Leucine regulates lipid metabolism in adipose tissue through adipokine-mTOR-SIRT1 signaling pathway and bile acid—microbe axis in a finishing pig model

作     者：Yunju Yin Saiming Gong Mengmeng Han Jingzun Wang Hanjing Shi Xianji Jiang Liu Guo Yehui Duan Qiuping Guo Qinghua Chen Fengna Li Yunju Yin;Saiming Gong;Mengmeng Han;Jingzun Wang;Hanjing Shi;Xianji Jiang;Liu Guo;Yehui Duan;Qiuping Guo;Qinghua Chen;Fengna Li

作者机构：Key Laboratory of Agro-ecological Processes in Subtropical RegionInstitute of Subtropical AgricultureChinese Academy of SciencesHunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic ProcessHunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production College of Animal Science and TechnologyHunan Agricultural University College of Modern Agricultural SciencesUniversity of Chinese Academy of Sciences College of Life Science and EngineeringNorthwest Minzu University 

出 版 物：《Animal Nutrition》 (动物营养(英文))

年 卷 期：2024年第16卷第1期

页      面：158-173页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 

基　　金：supported by National Natural Science Foundation of China (31972582,U22A20516) Science and Technology Innovation Program of Hunan Province (2021RC4039) Key R&D Program of Hunan Province (2022NK2026) the Youth Innovation Promotion Association CAS (Y202079) 

主　　题：Leu Lipid metabolism Gut microbiota Bile acid 

摘      要：This study was conducted to explore the regulatory mechanism of leucine (Leu) on lipid metabolism of finishing ***-four Duroc×Landrace×Large cross pigs with an average body weight of68.33±0.97 kg were randomly allocated into 3 treatment groups with 8 replicates per group (1 pig per replicate).The dietary treatments were as follows:control group (CON),0.25%Leu group and 0.50%Leu *** experimental period was 42 *** results showed as follows.(1) Compared with the CON,0.25%and 0.50%Leu increased (P0.01) the average daily gain (ADG),while the average backfat thickness (ABT) and the ratio of feed intake to body weight gain (F:G ratio) were decreased (P0.05).(2)In the 0.25%Leu group,the relative mRNA expression levels of sterol regulatory element binding protein-1c (SREBP1c),recombinant fatty acid transport protein 1 (FATP1),chemerin and peroxisome proliferatoractivated receptor γ (PPARγ) were decreased but the level of fatty acid binding protein 4 (FABP4) and fatty acid translocase (FAT/CD36) were increased in backfat *** the 0.25%Leu group,the protein levels of p-Rictor,p-Raptor,p-eIF4E-binding protein 1 (p-4EBP1),p-silent mating type information regulator 2homolog 1 (p-SIRT1) and acetylation ribosome s6 protein kinase 1 (Ac—S6K1) were increased (P0.05).(3) Compared to the CON,the diversity of gut microbiota in the 0.25%Leu group was *** component analysis showed that the relative abundance of Bacteroidetes,Lactobacillus and Desulfovibrio was higher in the 0.25%Leu group than the CON,but the relative abundance of Firmicutes,Treponema and Shigella was lower than in the CON (P0.05).(4) Four different metabolites were screened out from the serum of finishing pigs including allolithocholic acid (alloLCA),isolithocholic acid (isoLCA),ursodeoxycholic acid (UDCA) and hyodeoxycholic acid (HDCA),which correlate to various degrees with the above *** conclusion,Leu could promote adipose tissue lipolysis of finishing pigs throug