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juvenile hormone induces reproduction via miR-1175-3p in the red imported fire ant, Solenopsis invicta

Insect Science 2024年第2期31卷 371-386页

作者： Jie Chen Ziying Guan Yunjie Ma Qingxing Shi Ting Chen Muhammad Irfan WarisLihua Lyu Lihua Lyu Yongyue Lu Guojun Qi Institute of Plant Protection Guangdong Academy of Agricultural SciencesKey Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural AffairsGuangdong Provincial Key Laboratory of High Technology for Plant ProtectionGuangzhouChina Department of Entomology College of Plant ProtectionSouth China Agricultural UniversityGuangzhouChina

juvenile hormone(JH)acts in the regulation of caste differentiation between queens and workers(i.e.,with or without reproductive capacity)during vitellin synthesis and oogenesis in social ***,the regulatory mechanisms have not yet been ***,we identified a highly expressed microRNA(miRNA),miR-1175-3p,in the red imported fire ant,Solenopsis *** found that miR-1175-3p is prominently present in the fat bodies and ovaries of ***,miR-1175-3p interacts with its target gene,broad-complex core(Br-C),in the fat *** utilizing miR-1175-3p agomir,we successfully suppressed the expression of the Br-C protein in queens,resulting in reduced vitellogenin expression,fewer eggs,and poorly developed ***,decreasing miR-1175-3p levels led to the increased expression of Br-C and vitellogenin in workers,triggering the“re-development”of the ***,when queens were fed with JH,the expression of miR-1175-3p decreased,whereas the expression of vitellogenin-2 and vitellogenin-3 ***,the suppression of fertility in queens caused by treatment with agomir miR-1175-3p was completely rescued by the increased vitellogenin expression induced by being fed with *** results suggest the critical role of miR-1175-3p in JH-regulated reproduction,shedding light on the molecular mechanism underlying miRNA-mediated fecundity in social insects and providing a novel strategy for managing ***.

关键词： juvenile hormone miR-1175-3p red imported fire ant reproduction social insects vitellogenin

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juvenile hormone signaling-a mini review

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Insect Science 2019年第4期26卷 600-606页

作者： Kang Li Qiang-Qiang Jia Sheng Li Guangzhou Key Laboratory of Insect Development Regulation and Application Research Institute of Insect Science and Technology&School of Life SciencesSouth China Normal UniversityGuangzhouChina

Since it was first postulated by Wigglesworth in 1934, juvenile hormone (JH) is considered a status quo hormone in insects because it prevents metamorphosis that is initiated by the molting hormone 20-hydroxyecdysone (20E). During the last decade, significant advances have been made regarding JH signaling. First, the bHLH-PAS transcription factor Met/Gce was identified as the JH intracellular receptor. In the presence of JH, with the assistance of Hsp83, and through physical association with a bHLH?PAS transcriptional co-activator, Met/Gce enters the nucleus and binds to E-box-like motifs in promoter regions of JH primary?response genes for inducing gene expression. Second, the zinc finger transcription factor Kr-hl was identified as the anti-metamorphic factor which transduces JH signaling. Via Kr-hl binding sites, Kr-hl represses expression of 20E primary?response genes (i.e. Bi\ E93 and E5) to prevent 20E-induced metamorphosis. Third, through the intracellular signaling, JH promotes differ ent aspects of female reproduction. Nevertheless, this action varies greatly from species to species. Last, a hypothetical JH membrane receptor has been predicted to be either a GPCR or a tyrosine kinase receptor. In future, it will be a great challenge to understand how the JH intracellular receptor Met/Gce and the yet unidentified JH membrane receptor coordinate to regulate metamorphosis and reproduction in insects.

关键词： intracellular receptor juvenile hormone (JH) Kr-h 1 membrane receptor Met/Gce metamorphosis reproduction

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juvenile hormone acts on male accessory gland function via regulating L-asparaginase expression and triacylglycerol mobilization in Aedes aegypti

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Insect Science 2023年第1期30卷 81-94页

作者： Xiang-Yang Lyu Xue-Li Wang Dan-Qian Geng Hong Jiang Zhen Zou State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of ZoologyChinese Academy of SciencesBejingChina CAS Center for Excellence in Biotic Interactions University of Chinese Academy of SciencesBejingChina

hormones control the reproductive development of Aedes aegypti *** adult male reproductive process and mating behavior require adequate nutrients and *** the molecular mechanism linking hormones,energy metabolism,and reproduction in male mosquitoes is *** this study,we found that the size of the male accessory gland,an essential part of the male reproductive system,gradually increased after ***,it was significantly reduced in male mosquitoes deficient in methoprene-tolerant(Met),the receptor of juvenile ***,egg hatchability of females that mated with Met-depleted males showed the same downward *** mRNA level of the gene encoding accessory gland protein,l-asparaginase(ASNase),was reduced in Met dsRNA-treated *** mobility shift assay and quantitative reverse transcription-PCR results revealed that Met was capable of binding directly to the promoter of ASNase and activated its *** interference of ASNase in males resulted in the reduction of egg hatchability of the females with which they *** results showed that Met influenced the fecundity of male mosquitoes by directly upregulating the expression of the ASNase ***,the levels of triacylglycerol and the sizes of lipid droplets were decreased by 72-78 h after eclosion in the fat body cells,whereas both of them increased in Met-depleted male mosquitoes,indicating that Met knockdown reduced lipid *** data demonstrate that Met might influence the egg hatchability of females by regulating lipid metabolism and the development of the male accessory gland in male mosquitoes.

关键词： accessory gland protein energy juvenile hormone lipid metabolism male accessory gland male mosquito

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juvenile hormone regulates photoperiod-mediated male reproductive diapause via the methoprene-tolerant gene in the ladybeetle Harmonia axyridis

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Insect Science 2022年第1期29卷 139-150页

作者： Qiao Gao Bei Li Bing-Xin Wei Wen Liu Pan Wang Jia-Lu Wang Xing-Miao Zhou Xiao-Ping Wang Hubei Key Laboratory of Insect Resources Utilization and Sustainable Pest Management College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhan430070China Department of Plant Protection Wuhan Vegetable Research InstituteWuhan430065China

juvenile hormone (JH) absence induces photoperiod-mediated reproductive diapause, which is characterized by reproductive cessation. Although the role of methoprene-tolerant (Met)-mediated JH signaling in photoperiod-mediated female reproduction has been well documented, its role in male reproduction remains unclear. In this study, we investigated the role of JH in regulating photoperiod-mediated development of the male internal reproductive system (IRS) in the predatory ladybeetle Harmonia axyridis (Pallas). In a previous study, we found that adult male H. axyridis reared under either a short-day (SD) or long-day (LD) photoperiod had obvious differences in IRS development, but we were unable to identify the regulators of male reproductive diapause. In this study, we found that beetles reared under an SD photoperiod had significantly lower JH titer and a relatively undeveloped male IRS compared with those reared under an LD photoperiod. Additionally, application of the JH analog (JHA) methoprene promoted IRS development. Furthermore, Met knockdown strongly blocked JH signaling in males reared under the LD photoperiod, thereby slowing IRS development. Moreover, exogenous JHA did not reverse the suppressed development of the male IRS caused by Met knockdown. These results indicate that photoperiod regulates male IRS development in H. axyridis through a conserved Met-dependent JH signaling pathway.

关键词： Harmonia axyridis juvenile hormone male reproductive diapause methoprene-tolerant photoperiod RNAi

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Dual roles of juvenile hormone signaling during early oogenesis in Drosophila

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Insect Science 2020年第4期27卷 665-674页

作者： Wei Luo Sethuraman Veeran Jian Wang Sheng Li Kang Li Su-Ning Liu Guangzhou Key Laboratory of Insect Development Regulation and Application Research Institute of Insect Science and Technology&School of Life SciencesSouth China Normal UniversityGuangzhouChina and 2 Department of EntomologyUniversity of MarylandCollege ParkMDUSA

juvenile hormone(JH)signaling plays crucial roles in insect metamorphosis and *** of JH signaling in germline stem cells(GSCs)remains largely ***,we found that the number of GSCs significantly declined in the ovaries of Met,Gee and JHAMT *** we inhibited JH signaling in selected cell types of ovaries by expressing Met and Gee or Kr-hl double-stranded RNAs(dsRNAs)using different Gal4 *** of JH signaling in muscle cells has no effect on GSC *** of JH signaling in cap cells reduced GSCs *** expression of Met and Gee dsRNA but not Kr-hl by Nos-Gal4 increased GSC *** results indicate that JH signaling plays an important role in GSC maintenance.

关键词： cap cells germline stem cells juvenile hormone oogenesis ovary

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Expression of juvenile hormone acid O-methyltransferase and juvenile hormone synthesis in Blattella germanica

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Insect Science 2018年第5期25卷 787-796页

作者： Claudia V. Dominguez Jose L. Maestro Institute of Evolutionary Biology CSIC-Universitat Pompeu Fabra Barcelona Spain

juvenile hormone （JH）, a sesquiterpenoid synthetized by the insect corpora allata （CA）, plays critical roles in metamorphosis and reproduction. Penultimate or last step of JH synthesis is catalyzed by juvenile hormone acid O-methyltransferase （JHAMT）. Here we report the cloning and expression analysis of the JHAMT orthologue in the cockroach, Blattella germanica （L.）（BgJHAMT）. BgJHAMT is mainly expressed in CA, with only expression traces in ovary. Three different isoforms, differing in the 3＇-UTR sequence, were identified. Isoform A shows between 35 and 65 times higher expression than B and C in CA from penultimate nymphal instar and adult females. RNAi-triggered knock down of BgJHAMT produces a dramatic reduction of JH synthesis, concomitant with a decrease of fat body viteUogenin expression and basal follicle length. BgJHAMT mRNA levels in CA of females along the gonadotrophic cycle parallel, with a slight advancement, JH synthesis profile. BgJHAMT mRNA levels were reduced in starved females and in females in which we reduced nutritional signaling by knocking down insulin receptor and target of rapamycin （TOR）. Results show that conditions that modify JH synthesis in adult B. germanica females show parallel changes of BgJHAMT mRNA levels and that the JH-specifie branch of the JH synthesis pathway is regulated in the same way as the mevalonate branch. Furthermore, we demonstrate that nutrition and its signaling through the insulin receptor and TOR pathways are essential for activating BgJHAMT expression, which suggests that this enzyme can be a checkpoint for the regulation of JH production in relation to nutritional status.

关键词： Blattella germanica corpora allata JHAMT juvenile hormone nutritionalsignaling

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Methoprene does not affect juvenile hormone titers in honey bee （Apis mellifera） workers

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Insect Science 2018年第2期25卷 235-240页

作者： Zachary Y. Huang Stephanie Lin Kiheung Ahn Department of Entomology Michigan State University East Lansing Michigan USA Ecology Evolutionary Biology and Behavior Michigan State University East Lansing Michigan USA BEACON Center for the Study of Evolution in Action Michigan State University East Lansing Michigan USA and 4High School Honors Science Program 2007 Michigan State University East Lansing Michigan USA

Methoprene, a juvenile hormone （JH） analog, is a widely used insecticide that also accelerates behavioral development in honey bees （Apis mellifera）. JH regulates the transition from nursing to foraging in adult worker bees, and treatment with JH or methoprene have both been shown to induce precocious foraging. To determine how methoprene changes honey bee behavior, we compared JH titers ofmethoprene-treated and untreated bees. Behavioral observations confirmed that methoprene treatment significantly increased the number of precocious foragers in 3 out of 4 colonies. In only 1 out of 4 colonies, however, was there a significant difference in JH titers between the methoprene- treated and control bees. Further, in all 4 colonies, there was no significant differences in JH titers between precocious and normal-aged foragers. These results suggest that methoprene did not directly affect the endogenous JH secreted by corpora allata. Because methoprene caused early foraging without changing workers＇ JH titers, we conclude that methoprene most likely acts directly on the JH receptors as a substitute for JH.

关键词： behavioral development juvenile hormone analog juvenile hormone metho-prene precocious foraging

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Cloning and structural characterization of juvenile hormone diol kinase in Spodoptera litura

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Insect Science 2016年第6期23卷 819-828页

作者： Bao-Juan Zeng Yuan Lv Li-Li Zhang Li-Hua Huang Qi-Li Feng Guangzhou Key Laboratory of Insect Development Regulation and Application Research School of Life Sciences South China Normal University Guangzhou 510631 China

juvenile hormone （JH） is one of the key insect hormones that regulate metamorphosis. juvenile hormone diol kinase （JHDK） is an enzyme involved in JH metabolism and catalyzes JH diol to form a polar end product, JH diol phosphate that has no JH activity. In this study, a JHDK complementary DNA （cDNA） was cloned from Spodoptera litura and the structure and expression of the gene was characterized. The cDNA was 714 base pairs in length and encoded a protein of 183 amino acids with a molecular mass of 21 kDa and an isoelectric point of 4.55. Based on the structure, three putative calcium binding motifs and guanosine triphosphate-binding motifs were predicted in the protein. Modeling of the 3-D structure showed that the protein consisted of eight α-helixes linked with loops, with no β-sheets. The gene was expressed in the epidermis, fat body and midgut of fifth and sixth instar larvae. The expression level in the epidermis was lower than in the fat body and midgut. The gene was expressed at higher levels at the early stages than in the later stages of fifth and sixth instar midgut and fat body. The results suggest that this gene may be involved in the regulation of the JH titer in larvae ofS. litura.

关键词： juvenile hormone juvenile hormone diol kinase metabolism Spodoptera litura

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juvenile hormone membrane signaling phosphorylates USP and thus potentiates 20-hydroxyecdysone action in Drosophila

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Science Bulletin 2022年第2期67卷 186-197,M0004页

作者： Yue Gao Suning Liu Qiangqiang Jia Lixian Wu Dongwei Yuan Emma Y.Li Qili Feng Guirong Wang Subba R.Palli Jian Wang Sheng Li Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Institute of Insect Science and Technology&School of Life SciencesSouth China Normal UniversityGuangzhou 510631China Guangmeiyuan R&D Center Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied TechnologySouth China Normal UniversityMeizhou 514779China International Department The Afﬁliated High School of South China Normal UniversityGuangzhou 510631China Lingnan Guangdong Laboratory of Modern Agriculture Genome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China Department of Entomology College of AgricultureFood and EnvironmentUniversity of KentuckyLexington 40546USA Department of Entomology University of MarylandCollege Park 20742USA

juvenile hormone(JH) and 20-hydroxyecdysone(20 E) coordinately regulate development and metamorphosis in insects. Two JH intracellular receptors, methoprene-tolerant(Met) and germ-cell expressed(Gce), have been identified in the fruit fly Drosophila melanogaster. To investigate JH membrane signaling pathway without the interference from JH intracellular signaling, we characterized phosphoproteome profiles of the Met gce double mutant in the absence or presence of JH in both chronic and acute *** through a potential receptor tyrosine kinase and phospholipase C pathway, JH membrane signaling activated protein kinase C(PKC) which phosphorylated ultraspiracle(USP) at Ser35, the PKC phosphorylation site required for the maximal action of 20 E through its nuclear receptor complex Ec RUSP. The usp;mutant, in which Ser was replaced with Ala at position 35 by genome editing, showed decreased expression of Halloween genes that are responsible for ecdysone biosynthesis and thus attenuated 20 E signaling that delayed developmental timing. The usp;mutant also showed lower Yorkie activity that reduced body size. Altogether, JH membrane signaling phosphorylates USP at Ser35 and thus potentiates 20 E action that regulates the normal fly development. This study helps better understand the complex JH signaling network.

关键词： juvenile hormone Receptor tyrosine kinase Phosphoproteomics USP 20-Hydroxyecdysone action Yorkie activity

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In the monarch butterfly the juvenile hormone effect upon immune response depends on the immune marker and is sex dependent

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Open Journal of Ecology 2013年第1期3卷 53-58页

作者： Guadalupe Villanueva Humberto Lanz-Mendoza Salvador Hernandez-Martinez Minerva Sanchez Zavaleta Javier Manjarrez Jose MContreras-Garduno Jorge Contreras-Garduno Center of Research in Biotic Resources Autonomous University of Mexico StateTolucaMexico Center of Research in Infectious Diseases National Institute of Public HealthCuernavacaMexico Institute of Ecology National Autonomous University of MexicoMexico CityMexico Department of Biology University of GuanajuatoGuanajuatoMexico

In insects, juvenile hormone (JH) decreases or has any effect upon the phenoloxidase (PO) activity, and favors or decreases the Antimicrobial Peptides (AMPs) expression. Although there is no information about the differential effect of such hormone, two possibilities are that it depends on (a) the immune marker recorded and (b) sexual differences. Here, three commonly used immune markers, Phenoloxidase (PO), hydrogen peroxide (H2O2), and lytic activity, were measured 3, 6 and 24 hours after administration of methoprene (JHa, an analog of juvenile hormone) in male and female monarch butterflies (Danaus plexippus). At 3 and 6 h post-JHa administration, the PO activity increased in females but it only increased at 3 h in males, whereas H2O2 levels increased only in females at 3 h. For the remaining times the JHa had a null effect on POand H2O2. On the other hand, the JHa had a null effect for lytic activity in both sexes at 3, 6 and 24 h. To our knowledge, this is the first report of a positive effect of a JHa onPOand H2O2 and suggests that this effect is sex dependent.

关键词： Immune Response Life History juvenile hormone Reproductive Diapause Nitric Oxide

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