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Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica

Synthetic and Systems Biotechnology 2023年第4期8卷 716-723页

作者： Yan Zhang Xuejing Zhang Haiyan Liu Jin Hou Mengmeng Liu Qingsheng Qi State Key Laboratory of Microbial Technology Shandong UniversityQingdao266237China

2′-Fucosyllactose(2′-FL)has great application value as a nutritional component and the whole cell biosynthesis of 2′-FL has become the focus of current *** lipolytica has great potential in oligosaccharide synthesis and large-scale *** this study,systematic engineering of *** for efficient 2′-FL production was *** fusing different protein tags,the synthesis of 2′-FL was optimized and the ubiquitin tag was demonstrated to be the best choice to increase the 2′-FL *** iterative integration of the related genes,increasing the precursor supply,and promoting NADPH regeneration,the 2′-FL synthesis was further *** final 2′-FL titer,41.10 g/L,was obtained in the strain *** work reports the highest 2′-FL production in ***,and demonstrates that *** is an efficient microbial chassis for the synthesis of oligosaccharides.

关键词： 2′-Fucosyllactose Yarrowia lipolytica iterative integration Pathway enhancement NADPH regeneration

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Focusing properties of discrete RF quadrupoles

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Chinese Physics C 2017年第8期41卷 125-129页

作者：李智慧王志军 The Key Laboratory of Radiation Physics and Technology of Ministry of Education Institute of Nuclear Science and TechnologySichuan University Institute of Modern Physics Chinese Academy of Sciences

The particle motion equation for a Radio Frequency（RF） quadrupole is derived. The motion equation shows that the general transform matrix of a RF quadrupole with length less than or equal to 0.5βλ（β is the relativistic velocity of particles and λ is wavelength of radio frequency electromagnetic field） can describe the particle motion in an arbitrarily long RF quadrupole. By iterative integration, the general transform matrix of a discrete RF quadrupole is derived from the motion equation. The transform matrix is in form of a power series of focusing parameter B. It shows that for length less than βλ, the series up to the 2ndorder of B agrees well with the direct integration results for B up to 30, while for length less than 0.5βλ, the series up to 1storder is already a good approximation of the real solution for B less than 30. The formula of the transform matrix can be integrated into linac or beam line design code to deal with the focusing of discrete RF quadrupoles.

关键词： RF quadrupole transform matrix focusing parameter iterative integration hybrid RFQ

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