Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica

作     者：Yan Zhang Xuejing Zhang Haiyan Liu Jin Hou Mengmeng Liu Qingsheng Qi 

作者机构：State Key Laboratory of Microbial TechnologyShandong UniversityQingdao266237China 

出 版 物：《Synthetic and Systems Biotechnology》 (合成和系统生物技术（英文）)

年 卷 期：2023年第8卷第4期

页      面：716-723页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0836[工学-生物工程] 

基　　金：supported by the Key R&D Program of Shandong Province(2020CXGC010602) 

主　　题：2′-Fucosyllactose Yarrowia lipolytica Iterative integration Pathway enhancement NADPH regeneration 

摘      要：2′-Fucosyllactose(2′-FL)has great application value as a nutritional component and the whole cell biosynthesis of 2′-FL has become the focus of current *** lipolytica has great potential in oligosaccharide synthesis and large-scale *** this study,systematic engineering of *** for efficient 2′-FL production was *** fusing different protein tags,the synthesis of 2′-FL was optimized and the ubiquitin tag was demonstrated to be the best choice to increase the 2′-FL *** iterative integration of the related genes,increasing the precursor supply,and promoting NADPH regeneration,the 2′-FL synthesis was further *** final 2′-FL titer,41.10 g/L,was obtained in the strain *** work reports the highest 2′-FL production in ***,and demonstrates that *** is an efficient microbial chassis for the synthesis of oligosaccharides.