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isolation and screening of Oenococcus oeni and Its Medium Optimization

Agricultural Biotechnology 2021年第1期10卷 114-115,119页

作者： Dongdong HOU Xi HE College of Biological Engineering Qilu University of Technology(Shandong Academy of Sciences)Jinan 250353China

[Objectives] This study was conducted to further improve the application of Oenococcus oeni in the wine brewing industry. [Methods] O. oeni was isolated, screened, and identified, and medium optimization was performed to determine the best medium, temperature and oxygen conditions. [Results] O. oeni grew better in mFT80 medium, and the culture conditions were 25 ℃ and an anaerobic condition. [Conclusions] This study provides a reference for the development of China’s wine industry.

关键词： Oenococcus oeni isolation and screening Culture medium Optimization

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Study on isolation and Degradation Characteristics of an Aniline-degrading Strain

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Meteorological and Environmental Research 2013年第4期4卷 46-48页

作者： Shimin Zhang Di Zhao Shuxia Xu Cuicui Liu Kun Wu College of Life Sciences Henan Agricultural University Key Laboratory of Enzyme Engineering of Agricultural Microbiology Ministry of Agriculture

[Objective] The research aimed to screen out an efficient aniline-degrading strain and study its degradation characteristics. [Method] By domesticated enrichment culture, an efficient aniline-degrading strain named as DA-K was isolated from activated sludge sample collected from a chemical plant in Henan. DA-K could use aniline as the sole carbon and nitrogen sources. The strain was carried out physiological and biochemi- cal identification, and its biological degradation characteristics were studied. [ Result] DA-K strain was G - and red-shaped, and its colony color was off-white. It was initially determined as Acinetobacter sp. By measuring, the optimum growth temperature and pH for DA-K were respectively 30 ~C and 6.0. DA-K strain could grow well in inorganic salt medium with aniline of 2 500 mg/L. After shaking for 96 h under the conditions of pH 6.0, 30 ℃, 180 r/min and 1 000 mg/L of aniline, the degradation rate of the aniline by DA-K could reach nearly 80%. [ Conclusion] The DA-K strain had a higher aniline degradation efficiency and actual treatment capability of the aniline wastewater, which laid foundation for establishing gene engineerincl strain.

关键词： Aniline-degrading strain isolation and screening Degradation characteristics China

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