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Antibiotic sensitivity pattern of bacterial pathogens in the intensive care unit of Fatmawati Hospital,Indonesia

Asian Pacific Journal of Tropical Biomedicine 2011年第1期1卷 39-42页

作者： Maksum Radji Siti Fauziah Nurgani Aribinuko Laboratory of Microbiology and Biotechnology Department of PharmacyFaculty of Mathematics and ScienceUniversity of Indonesia Department of Intensive Care Unit Fatmawati Hospital

Objective:To evaluate the sensitivity pattern of bacterial pathogens in the intensive care unit(ICU) of a tertiary care of Falmawati Hospital Jakarta ***:A cross sectional retrospective study of bacterial pathogen was carried out on a total of 722 patients that were admitted to the ICU of Fatmawati Hospital Jakarta Indonesia during January 2009 to March 2010. All bacteria were identified by standard microbiologic methods,and(heir antibiotic susceptibility testing was performed using disk diffusion ***:Specimens were collected from 385 patients who were given antimicrobial treatment,of which 249(64.68%) were cultured positive and 136(35.32%) were *** most predominant isolate was Pseudomonas aeruginosa(***)(26.5%) followed by Klebsiella pneumoniae(***)(15.3%) and Staphylococcus epidermidis(14.9%).*** isolates showed high rate of resistance to cephalexin(95.3%),cefotaxime(64.1%),and ceftriaxone(60.9%).Amikacin was the most effective(84.4%) antibiotic against *** followed by imipenem(81.2%),and meropenem(75.0%).*** showed resistance to cephalexin(86.5%),ceftriaxone(75.7%),ceftazidime(73.0%),cefpirome(73.0%) and cefotaxime(67.9%),***:Most bacteria isolated from ICU of Fatmawati Hospital Jakarta Indonesia were resistant to the third generation of cephalosporins,and quinolone *** surveillance of antibiotic susceptibility pallerns is very important for setting orders to guide the clinician in choosing empirical or directed therapy of infected patients.

关键词： Antibiotic susceptibility Intensive care unit Bacterial Resistance Bacterial pathogen Sensitivity pattern Antimicrobial Resistance Pseudomonas aeruginosa Klebsiella pneumoniae isolate Specimen

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Microbiological assessment of indoor air of a teaching hospital in Nigeria

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Asian Pacific Journal of Tropical Biomedicine 2012年第6期2卷 465-468页

作者： Awosika SA Olajubu FA Amusa NA Department of Microbiology Olabisi Onabanjo University Department of Medical Microbiology & Parasitology Olabisi Onabanjo University Teaching Hospital Department of Plant Science & Applied Zoology Olabisi Onabanjo University

Objective:To investigate the quality of indoor air of different wards and units of Olabisi Onabanjo University Teaching Hospital, Sagamu, to ascertain their contribution to infection rate in the ***:The microbial quality of indoor air of nine wards/units of Olabisi Onabanjo University Teaching Hospital, Sagamu, Nigeria was conducted. Sedimentation technique using open Petri-dishes containing different culture media was employed and samplings were done twice daily, one in the morning shortly after cleaning and before influx of people/patients into the wards/units and the other in the evening when a lot of activities would have taken place in these wards. isolates were identified according to standard ***:Results showed that there was a statistically significant difference(氈2= 6.0167) in the bacteria population of the different sampling time whereas it was not so for fungi population(氈2= 0.2857). Male medical ward(MMW) and male surgical general(MSG) recorded the highest bacterial and fungal growth while the operating theatre(OT) was almost free of microbial burden. The bacteria isolates were Staphylococcus aureus, Klebsiellasp., Bacillus cereus, Bacillus subtilis, Streptococcus pyogenes andSerratia marscenceswhile the fungi isolates includedAspergillus flavus, Penicilliumsp.,Fusariumsp.,Candida *** aureuswas the predominantly isolated bacterium whilePenicilliumsp. was the most isolated ***:Though most of the microbial isolates were potential and or opportunistic pathogens, there was no correlation between the isolates in this study and the surveillance report of nosocomial infection during the period of study, hence the contribution of the indoor air cannot be established. From the reduction noticed in the morning samples, stringent measures such as proper disinfection and regular cleaning, restriction of patient relatives' movement in and out of the wards/units need to be enforced so as to imp

关键词： Indoor air OOUTH Open-plate technique Nosocomial infections Staphylococcus aureus Microbiological assessment Microbial quality Bacterial isolate Fungal isolate Opportunistic pathogen Nosocomial infection Infection rate Microbial isolate Penicilliumsp Sedimentation technique

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Antibacterial activity of Lawsonia inermis Linn(Henna) against Pseudomonas aeruginosa

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Asian Pacific Journal of Tropical Biomedicine 2011年第3期1卷 173-176页

作者： Habbal O Hasson SS El-Hag AH Al-Mahrooqi Z Al-Hashmi N Al-Bimani Z MS Al-Balushi Al-Jabri AA Department of Human & Clinical Anatomy College of Medicine and Health Sciences.Sultan Qaboos University Division of Immunology Department of Microbiology and ImmunologyCollege of Medicine and Health Sciences.Sultan Qaboos University Division of Microbiology Department of Microbiology and ImmunologyCollege of Medicine and Health SciencesSultan Qaboos University

Objective:To investigate the antibacterial activity of henna(Lawsonia inermis Linn) obtained from different regions of Oman against a wide array of ***:fresh henna samples were obtained from different regions of Oman as leaves and seeds,100 g fresh and dry leaves and SO g of fresh and dry seeds were separately soaked in 500 mL of ethanol for three days,respectively,with frequent *** mixture was filtered,and the crude extract was *** crude extract was then heated,at 48 ℃ in a water bath to evaporate its liquid *** dry crude henna extract was then tested for its antibacterial activity using well-diffusion antibiotic susceptibility *** extracts were investigated for their antibacterial activity at different concentrations against a wide array of different micro-organisms including a laboratory standard bacterial strain of Pseudomonas aeruginosa(NCTC 10662)(A aeruginosa) and eleven fresh clinical isolates of *** obtained from patients attending the Sultan Qaboos University Hospital(SQUH).2-Hydroxy-p-Nathoqinone-Tech(2-HPNT, MW=174.16,C_(10)H_40_3) was included as control(at 50%concentration) along with the henna samples ***:Henna samples demonstrated antibacterial activity against all isolates but the highest susceptibility was against *** with henna samples obtained from Al-sharqyia ***:Omani henna from Al-sharqyia region demonstrates high in vitro anti-P. aeruginosa activity compared with many henna samples from different regions of Oman.

关键词： Pseudomonas aeruginosa Lawsonia inermis Linn Henna Antibacterial activity Oman Antibiotic susceptibility Micro-organism Bacterial strain Clinical isolate Crude extract

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A new species in the genus Hebarditettix(Orthoptera:Tetrigoidea: Scelimenidae) from the Lengshuihe Nature Preserve, Guizhou

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Entomotaxonomia 2016年第1期38卷 5-9页

作者：查岭生于凤明文庭池 Kevin D.HYDE 淮北师范大学生命科学学院安徽淮北235000 贵州大学西南药用生物资源教育部工程研究中心贵州贵阳550025 Institute of Excellence in Fungal Research School of Science Mae Fah Luang University

In identifying the insect specimens collected from the Lengshuihe Nature Preserve, Guizhou, a new species of the genus Hebarditettix, H. vallis Zha Wen sp. nov. was found. We describe it and provide illustrations. It is similar to H. armatus（Hancock） but differs from the latter by： 1） antennae inserted distinctly below lower margins of eyes, the longest segment only 6 times as long as wide; 2） abbreviated carinae between shoulders inconspicuous; 3） lower margin of fore femur with two small teeth, mid femur as wide as visible part of tegmen; 4） hind process of pronotum short, only reaching two-fifths of hind tibia. An updated key to all species of the genus Hebarditettix is given.

关键词： Scelimeninae taxonomy ecology Karst landform isolate key

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Fox- and raccoon-dog–associated rabies outbreaks in northern China

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Virologica Sinica 2014年第5期29卷 308-310页

作者： Ye Liu Shoufeng Zhang Jinghui Zhao Fei Zhang Nan Li Hai Lian Wurengege Shiyu Guo Rongliang Hu Key Laboratory of Jinlin Provincial Zoonosis Laboratory of Epidemiology Veterinary Research Institute Academy of Military Medical Sciences Department of Physiology Hetao College Department of Physiology School of Medicine Showa University

Dear Editor,Rabies is a generally fatal disease caused by the rabies virus(RABV),and is transmitted mainly by Carnivora and Chiroptera(Fooks A R,et al.,2014;Tao X,et al.,2013).In China,stray dogs and some wild animals(e.g.,Chinese ferret badgers,foxes,and raccoon dogs)are the principal reservoirs for RABV(Hu R L,et al.,2009).Historically,rabies in wild foxes and raccoon dogs(Nyctereutes procyonoides)was recorded in the early

关键词： rabies Fox reservoirs fatal transmitted isolate cattle Changchun Heilongjiang surveillance

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Production of low cost <i>Bacillus thuringiensis</i>based-biopesticide for management of chickpea pod-borer <i>Helicoverpa armigera</i>(Huebn) in Pakistan

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Natural Science 2013年第11期5卷 1139-1144页

作者： Abida Bibi Khalique Ahmed Najma Ayub Sadia Alam Department of Microbiology Quaid-i-Azam University Islamabad Pakistan Departmentof Microbiology University of Haripur Haripur Pakistan KarakuramUniversity Gilgit Pakistan

The biopesticide was prepared from locally available low cost ingredients: dried beef blood, molasses and mineral salts (ZnCl2, MgCl2, MnCl2, CaCl2CaCl2, and FeCl3) which were used as medium for the laboratory scale production of Bacillus thuringiensis (B.t.) bio-pesticide by shake flask technique. Indigenous B.t. isolate PA-Sb- 46.3 which produced two crystals—bi-pyramidal and cuboidal was found 73.6 times toxic against H. armigera than reference strain Bacillus thuringiensis var. kurstaki (HD-I-S-1980) used. Medium was fermented for 72 hours at 30℃ ± 2℃ and 160 rpm. 72 h fermented medium showed 95% - 99% sprulation, with spore yield of 3.97 × 109 spores/ml, and LC50 value to 1st instar larvae of H. armigera was 0.53 μg/ml diet. Preservatives and diluents used in the biopesticide were found to be effective to store at room temperature over a period of 30 months. These observations suggested that the biopesticide produced was effective and highly economical for the industrial scale production to manage H. armigera in Pakistan.

关键词： Beef Blood Molasses Indigenous B.t. isolate PA-Sb-46.3 Sporulation Entomotoxicity Biopesticide

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ISOLATION AND STRUCTURE OF A NATURAL METALLIC COMPOUND—ZINCPOLYANEMINE

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Chinese Science Bulletin 1980年第5期25卷 444-445页

作者：徐炳祥韩公羽王肖鹏刘明珠许学余孟利宁陈仲良朱大元 second Military Medical College Shanghai Shanghai Institute of Materia Medica Academia Sinica

The Chinese medicinal plant Polyalthia nemoralis A. DC. (Anonaceae) grows in the south of Guangdong Province and Hainan Island. Its roots are often used by the natives of Hainan as a traditional cure for malaria and h... 详细信息

关键词： attempt Guangdong isolate Island medicinal roots designated yellow 下甲 starting

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ISOLATION, CULTIVATION AND INFECTIVITY OF ACTINORHIZAL ENDOPHYTE OF Casuarina equisetifolia

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Chinese Science Bulletin 1985年第6期30卷 833-838页

作者：蒋建德朱宝琴 Institute of Forestry and Soil Science Academia Sinica Shenyang Institute of Forestry and Soil Science Academia Sinica Shenyangince actinorhizal endophyte was first successfully isolated in 1978 various strains of Frankia have been isolated from nodules of Comptonia Alnus Elaeagnus Hippophae Myrica etc. by using different techniques. But still many other attempts to isolate Fran-

Since actinorhizal endophyte was first successfully isolated in 1978, various strains of Frankia have been isolated from nodules of Comptonia, Alnus, Elaeagnus, Hippophae, Myrica, etc. by using different techniques. B... 详细信息

关键词： Casuarina ISOLATION endophyte nodules Myrica isolate Elaeagnus Alnus glycerol sterile

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A novel technique for isolating orchid mycorrhizal fungi

A novel technique for isolating orchid mycorrhizal fungi

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作者：朱国胜喻子牛 State Key Laboratory of Agricultural Microbiology Huazhong Agricultural University Guizhou Key Laboratory of Agricultural Biotechnology Institute ofBiotechnology Guizhou Academy of Agricultural Science

We describe a technique for isolating mycorrhizal fungi from roots of *** technique involves selection and treatment of roots,preparation of pelotons,treatment of pelotons,culture of pelotons so that fungal hyphae grow out and strain *** technique is considered better because 1) problems of fungal and bacterial contamination are resolved,2) endophytic bacteria are suppressed and also used to promote hyphal growth from the pelotons,3) live and dead pelotons,and those from which fungi are culturable or unculturable can easily be identified,providing increased isolation efficiency,4) a single taxon can be isolated from a single peloton containing several mycorrhizal taxa,5) slow-growing mycorrhizal taxa can easily be *** implications and potential use of this technique in future studies is discussed.

关键词： Ceratorhiza,Epulorhiza isolate Monilioposis mycorrhiza orchid peloton

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Identification of human genomic DNA from suppressed metastatic phenotype cells of mouse lung adenocarcinoma

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中国实验血液学杂志 1997年第3期5卷 294-295页

作者： Xueming Ge, Yinglin Lu, Shengfa Fu, Kun Chen, Wenhong Fan, Shuang LiuDepartment of Molecular Pathologv, Beijing Institute of Basic Medical Sciences, Beijing 100850

Objectives:To isolate tumor metastaticsuppressing genes or relating human DNA sequencesand to study the molecular biological regulatingmechanism of tumor metastasis. Methods: HumanDNA fragments were amplified by Inter Alu PCRtechnique from normal human genomic DNAtransfected mouse tumor cell clones of which themetastatic phenotype had been suppressed. Thehuman origin of the amplified DNA was furtherconfirmed by PCR in situ hybridization. One

关键词： metastatic suppressed adenocarcinoma phenotype genomic DNA metastasis isolate suppressing similarity

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