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Quantitative Assessment of Amino Acid Damage upon keV ion beam irradiation Through FTIR Spectroscopy

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Plasma Science and Technology 2010年第3期12卷 378-384页

作者：黄青柯志刚苏玺袁航张束清余增亮 Key Laboratory of ion beam Bio-engineering Institute of Plasma Physics of Chinese Academy of Sciences

ion beam irradiation induces important biological effects and it is a long-standing task to acquire both qualitative and quantitative assessment of these effects. One effective way in the investigation is to utilize Fourier transformation infrared （FTIR） spectroscopy because it can offer sensitive and non-invasive measurements. In this paper a novel protocol was employed to prepare biomolecular samples in the form of thin and transversely uniform solid films that were suitable for both infrared and low-energy ion beam irradiation experiments. Under the irradiation of N^＋ and Ar^＋ ion beams of 25 keV with fluence ranging from 5×10^15 ions/cm^2 to 2.5×10^16 ions/cm^2, the ion radio-sensitivity of four amino acids, namely, glycine, tyrosine, methionine and phenylalanine, were evaluated and compared. The ion beam irradiation caused biomolecular decomposition accompanied by molecular desorption of volatile species and the damage was dependent on ion type, fiuence, energy and types of amino acids. The effectiveness of application of FTIR spectroscopy to the quantitative assessment of biomolecular damage dose effect induced by low-energy ion radiation was thus demonstrated.

关键词： ion beam irradiation amino acids IR spectroscopy

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Study on Screening of TaGA2ox1 Mutants in Wheat by ion beam irradiation

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Plasma Science and Technology 2010年第6期12卷 757-760页

作者：陈秋芳押辉远秦广雍焦浈 Henan Provincial Key Laboratory of ion beam Bio-engineering Zhengzhou University College of Life Sciences Luoyang Normal University

As a kind of mutagen, ion beam irradiation can create abundant biological mutations. A population of about 2000 lines was generated by irradiating dry wheat seeds of XiaoYan 81 with low-energy nitrogen ion beams. The traits of the plant, such as height, spike type, fertility, stem color and awn length, were investigated. The mutation rate in terms of the plant height in M2 was 2.9%. Eighteen deletion mutants of TaGA2ox1 were obtained. Associate analysis showed that TaGA2ox1 was closely related to the plant height. Most of the TaGA2ox1-deleted mutants were higher than the control, suggesting that the biological function of TaGA2ox1 is similar to its homologues in other plants. These results demonstrate that ion beam irradiation is an efficient tool in the construction of a mutant library for wheat.

关键词： ion beam irradiation wheat mutant TaGA2ox1

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Morphological and biochemical responses of Oryza sativa L.(cultivar MR219) to ion beam irradiation

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第12期14卷 1132-1143页

作者： Anna Pick Kiong LING Ying Chian UNG Sobri HUSSEIN Abdul Rahim HARUN Atsushi TANAKA Hase YOSHIHIRO Division of Human Biology International Medical University (IMU)57000 Kuala Lumpur Malaysia Department of Science Faculty of Engineering and Science Universiti Tunku Abdul Rahman53300 Setapak Kuala Lumpur Malaysia Agrotechnology and Bioscience Division Malaysian Nuclear Agency Bangi43000 Kajang Selangor Malaysia Radiation-Applied Biology Division Quantum Beam Science Directorate Japan Atomic Energy Agency 1233 Watanuki-machiTakasaki Gunma 370-1292 Japan

Objective: Heavy ion beam, which has emerged as a new mutagen in the mutation breeding of crops and ornamental plants, is expected to result in the induction of novel mutations. This study investigates the morphological and biochemical responses of Oryza sativa toward different doses of carbon ion beam irradiation. Methods: In this study, the dry seeds of O. sativa were irradiated at 0, 20, 40, 60, 80, 100, and 120 Gy, followed by in-vitro germination under controlled conditions. Morphological and biochemical studies were conducted to investigate the morphological and physiological responses of O. sativa towards ion beam irradiation. Results: The study demonstrated that low doses(10 Gy) of ion beam have a stimulating effect on the height, root length, and fresh weight of the plantlets but not on the number of leaves. Meanwhile, doses higher than 10 Gy caused reductions in all the morphological parameters studied as compared to the control samples. The highest total soluble protein content [(2.11±0.47) mg/g FW] was observed in plantlets irradiated at 20 Gy. All irradiated plantlets were found to have 0.85% to 58.32% higher specific activity of peroxidase as compared to the control samples. The present study also revealed that low doses of ion beam(10 and 20 Gy) had negligible effect on the total chlorophyll content of O. sativa plantlets while 40 Gy had a stimulating effect on the chlorophyll content. Plantlets irradiated between 40 to 120 Gy were shown to be 0.38% to 9.98% higher in total soluble nitrogen content which, however, was not significantly different from the control samples. Conclusions: Carbon ion beam irradiation administered at low to moderate doses of 10 to 40 Gy may induce O. sativa mutants with superior characteristics.

关键词： In vitro mutagenesis ion beam irradiation Total chlorophyll content Total soluble protein content Mutation breeding

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SQUARE HOLE FABRICATion IN MICRO NANO SCALE BY LOW ENERGY ECR ion beam irradiation

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SQUARE HOLE FABRICATION IN MICRO NANO SCALE BY LOW ENERGY EC...

Modification of structural and dielectric properties of polycrystalline Gd-doped BFO–PZO

2006年中国机械工程学会年会暨中国工程院机械与运载工...

作者： Shahjada Ahmed Pahlovy Sadao Momota M.Kashihara K.Nishimura Intelligent Mechanical System Engineering Department Kochi University of Technology Kochi-782-8502 Japan Intelligent Mechanical System Engineering Department Kochi University of Technology Kochi-782-8502 Japan Intelligent Mechanical System Engineering Department Kochi University of Technology Kochi-782-8502 Japan Intelligent Mechanical System Engineering Department Kochi University of Technology Kochi-782-8502 Japan

The importance of micro nano fabrication in nanotechnology world becoming greater and greater. Now a days several industries and technology especially IC industry, MEMS, solar cells, optoelectronics a

关键词： Electron Cyclotron Resonance ion beam irradiation mechanical properties.

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Journal of Advanced Dielectrics 2018年第5期8卷 1-21页

作者： Ambika Ray Banarji Behera Tanmoy Basuy Saumitra Vajandar Santosh Kumar Satpathy Pratibindhya Nayak Material Research Laboratory School of Physics Sambalpur UniversityJyoti Vihar BurlaOdisha 768019India Centre for Advanced 2D Materials National University of Singapore Singapore 117546Singapore Centre for ion beam Applications National University of Singapore Singapore 117542Singapore Department of Physics School of Applied Science Centurion University of Technology and Management BhubaneswarOdisha 752050India

(1-y)(BiFe_(1-x)GdxO_(3))–y(PbZrO3)composites(y=0:5),having four different Gd concentrations(x=0:05,0.1,0.15,and 0.2),were synthesized and their structural,dielectric,and ferroelectric properties have been studied using different characterization *** addition,to investigate the effect of ion implantation on the microstructure and dielectric properties,these composites were exposed to 2 MeV He+-*** of the structure,surface morphology and electrical properties of the samples before and after ion exposure were demonstrated using powder X-ray diffraction(XRD),scanning electron microscopy(SEM)technique,and LCR *** compositional analysis was carried out using energy dispersive X-ray spectrometry(EDS).XRD results demonstrated a decrease in the intensity profile of the dominant peak by a factor of 6 showing a degradation of the ***–Hall(WH)plots reveal reduction in the grain size after irradiation along with an increase in strain and dislocation density.A decrease in the dielectric constant and loss has been recorded after ion beam exposure with reduction in ac conductivity *** contribution of grain and grain boundary effect in conduction mechanism has been addressed using Nyquist *** the samples demonstrate a lossy ferroelectric loop which shows a clear modification upon *** role of structural defects modifying the physical properties of the composite materials is discussed in this work.

关键词： ion beam irradiation defects dielectric properties ferroelectricity.

Distribution of implanted ions in seeds and roots of mung bean

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Nuclear Science and Techniques 1998年第3期 53-55页

作者： Liu Dong Hua, Wang Wei, Jiang Wu Sheng, Zhang Zhi Xiang ( Department of Biology, Tianjin Normal University, Tianjin 300074) Hou Wen Qiang ( College of Life Sciences, Nankai University, Tianjin 300071) Guo Xi Ming and Li Yi ( Department of Phy DepartmentofBiology TianjinNormalUniversityTianjin300074 CollegeofLifeSciences NankaiUniversityTian

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关键词： Phaseolus radiatus (L.) Copper ion Iron ion ion beam irradiation ion distribution

Advance in the Growth of Ordered Ge/Si Quantum Dots

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Advance in the Growth of Ordered Ge/Si Quantum Dots