Quantitative Assessment of Amino Acid Damage upon keV Ion Beam Irradiation Through FTIR Spectroscopy

作     者：黄青 柯志刚 苏玺 袁航 张束清 余增亮 

作者机构：Key Laboratory of Ion Beam Bio-engineeringInstitute of Plasma Physics of Chinese Academy of Sciences 

出 版 物：《Plasma Science and Technology》 (等离子体科学和技术（英文版）)

年 卷 期：2010年第12卷第3期

页      面：378-384页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0805[工学-材料科学与工程（可授工学、理学学位）] 0702[理学-物理学] 

基　　金：supported by National Natural Science Foundation of China (No.10975152) the Key Innovative Project of Chinese Academy of Sciences (No.KJCX2-YW-N34-1) the Hundred Talents Program of the Chinese Academy of Sciences the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry 

主　　题：ion beam irradiation amino acids IR spectroscopy 

摘      要：Ion beam irradiation induces important biological effects and it is a long-standing task to acquire both qualitative and quantitative assessment of these effects. One effective way in the investigation is to utilize Fourier transformation infrared （FTIR） spectroscopy because it can offer sensitive and non-invasive measurements. In this paper a novel protocol was employed to prepare biomolecular samples in the form of thin and transversely uniform solid films that were suitable for both infrared and low-energy ion beam irradiation experiments. Under the irradiation of N^＋ and Ar^＋ ion beams of 25 keV with fluence ranging from 5×10^15 ions/cm^2 to 2.5×10^16 ions/cm^2, the ion radio-sensitivity of four amino acids, namely, glycine, tyrosine, methionine and phenylalanine, were evaluated and compared. The ion beam irradiation caused biomolecular decomposition accompanied by molecular desorption of volatile species and the damage was dependent on ion type, fiuence, energy and types of amino acids. The effectiveness of application of FTIR spectroscopy to the quantitative assessment of biomolecular damage dose effect induced by low-energy ion radiation was thus demonstrated.