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Clinicobiochemical investigations of gangrenous mastitis in does:immunological responses and oxidative stress biomarkers

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第1期14卷 33-39页

作者： Wael M.EL-DEEB Department of Clinical Studies College of Veterinary Medicine and Animal ResourcesKing Faisal University Department of Veterinary Medicine Infectious Diseases and Fish DiseasesFaculty of Veterinary MedicineMansoura University

A total of 50 does were used to determine selected hematological and biochemical parameters with special references to oxidative stress markers, acute phase protein profiles, and proinflammatory cytokines in healthy and gangrenous mastitis affected does. Animals were divided into two equal groups represented as clinically healthy （control） and diseased groups, respectively. The bacteriological examination of milk samples from diseased does revealed many types of bacterial infection. The isolated bacteria were Staphy/ococcus aureus （N=23/25）, Escherichia coil （N=11/25）, and Clostridium perfringens （N=4/25）. There was a significant increase in the levels of β-hydrexybutyrate, non-esterified free fatty acids, triglyceride, low density lipoprotein cholesterol （LDL-C）, aspartate aminotransferase, and alanine aminotransferase and a significant reduction in the levels of glucose, cholesterol, and high density lipoprotein cholesterol （HDL-C） in does with gangrenous mastitis compared to healthy does. Moreover, there was a significant increase in the levels of malondialdehyde and uric acid with a significant decrease in the levels of reduced glutathione, super oxide dismutase, and catalase in does with gangrenous mastitis compared to healthy does. In addition, there was a significant increase in the haptoglobin, serum amyloid A, fibrinogen, interleukin 6 （IL-6）, IL-1β, and tumor necrosis factor-a （TNF-a） in does with gangrenous mastitis compared to healthy ones. Conclusively, oxidative stress biomarkers, acute phase proteins, and proinflammatory cytokines play an essential task as biomarkers for gangrenous mastitis in does. Mastitis may be considered as one of the ketotic stressors in does after parturition.

关键词： Haptoglobin Serum amyloid A Malondialdehyde interleukin 1β interleukin 6 Tumor necrosis factor-a

Neutralizing peripheral circulating IL1β slows the progression of ALS in a lentivirus-infected OPTN^(E478G)mouse model

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Animal Models and Experimental Medicine 2023年第1期6卷 18-25页

作者： Wen-Bao Hu Xin Wang Zhi-Lin Pang Ran Duan Chun-Gu Hong Zheng-Zhao Liu Xiangya Hospital Central South UniversityChangshaChina

Background:Amyotrophic lateral sclerosis(ALS)is irreversible and fatal within 3-5 years,with limited options for *** is imperative to develop a symptom-based treatment that may increase the survival of ALS patients and improve their quality of *** status,especially elevated interleukin 1β(IL1β),has been reported to play a critical role in ALS *** study determined that neutralizing circulating IL1βslows down the progression of ALS in an ALS mouse ***:The ALS mouse model was developed by microinjection of lentivirus-carrying OPTN^(E478G)(optineurin,a mutation from ALS patients)into the intra-motor cortex of *** circulating IL1βwas neutralized by injecting anti-IL1βan-tibody into the tail ***-linked immunosorbent assay(ELISA)and real-time polymerase chain reaction(RT-PCR)were carried out to determine the protein and gene expression levels of IL1β.TUNEL assay was used to assess the neural cell *** staining of MAP2 and CASP3 was accomplished to evaluate neuronal cell *** fibrillary acidic protein staining was performed to ana-lyze the number of *** test,grip strength test,balance beam test,and footprint test were conducted to assess the locomotive function after anti-IL1β***:The model revealed that neuroinflammation contributes to ALS *** mice exhibited elevated neuroinflammation and IL1β*** anti-IL1βtreatment,ALS mice revealed decreased neural cell death and astrogliosis and gained improved muscle strength and motor ***:Blocking IL1βis a promising strategy to slow down the progression of ALS.

关键词： amyotrophic lateral sclerosis inflammation interleukin 1β neurodegeneration optineurin

Molecular characterization of an IL-1β gene from the large yellow croaker（Larimichthys crocea） and its effect on fish defense against Vibrio alginolyticus infection

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Zoological Research 2015年第3期36卷 133-141页

作者： Jun WU Yu-Hong SHI Xue-Heng ZHANG Chang-Hong LI Ming-Yun LI Jiong CHEN Laboratory of Biochemistry and Molecular Biology School of Manne Sciences Ningbo University Ningbo 315211 China Donghai Sea Collaborative Innovation Center for Industrial Upgrading Mariculture Ningbo University Ningbo 315211 China

interleukin 1β（IL-1β）, the first interleukin to be characterized, plays a key role in regulating the immune response. In this study, we determined the c DNA and genomic DNA sequences of the IL-1β gene from the large yellow croaker, Larimichthys crocea. Phylogenetic analysis indicated that the IL-1β（Lc IL-1β） gene was most closely related to that of European seabass（Dicentrarchus labrax）, sharing 67.8% amino acid identity. In healthy large yellow croaker, Lc IL-1β transcription was detected in all tested tissues, with the highest level found in the head kidney. Upon Vibrio alginolyticus infection, Lc IL-1β transcription in all tested tissues was significantly upregulated. Intraperitoneal injection of recombinant Lc IL-1β（r Lc IL-1β） improved the survival rate and reduced the tissue bacterial load after V. alginolyticus infection. In addition, r Lc IL-1β induced monocytes/macrophages（MO/MΦ） chemotaxis and increased phagocytosis and bactericidal activity in vitro. These results suggest that Lc IL-1β plays an important role in the large yellow croaker immune response against V. alginolyticus.

关键词： interleukin 1β Large yellow croaker Survival rate Vibrio alginolyticus Monocytes/macrophages

Plumbagin Inhibits Inflammatory Response and Bone Loss in Experimental Chronic Peridontitis Rats

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Plumbagin Inhibits Inflammatory Response and Bone Loss in Ex...