Molecular characterization of an IL-1β gene from the large yellow croaker（Larimichthys crocea） and its effect on fish defense against Vibrio alginolyticus infection

作     者：Jun WU Yu-Hong SHI Xue-Heng ZHANG Chang-Hong LI Ming-Yun LI Jiong CHEN 

作者机构：Laboratory of Biochemistry and Molecular Biology School of Manne Sciences Ningbo University Ningbo 315211 China Donghai Sea Collaborative Innovation Center for Industrial Upgrading Mariculture Ningbo University Ningbo 315211 China 

出 版 物：《Zoological Research》 (动物学研究（英文）)

年 卷 期：2015年第36卷第3期

页      面：133-141页

核心收录：

学科分类：1002[医学-临床医学] 0906[农学-兽医学] 09[农学] 

基　　金：supported by the National 863 Project(2012AA10A403) the Natural Science Foundation of Ningbo City of China(2014A610187) the Scientific Research Foundation of Graduate School of Ningbo University(G14041) 

主　　题：Interleukin 1β Large yellow croaker Survival rate Vibrio alginolyticus Monocytes/macrophages 

摘      要：Interleukin 1β（IL-1β）, the first interleukin to be characterized, plays a key role in regulating the immune response. In this study, we determined the c DNA and genomic DNA sequences of the IL-1β gene from the large yellow croaker, Larimichthys crocea. Phylogenetic analysis indicated that the IL-1β（Lc IL-1β） gene was most closely related to that of European seabass（Dicentrarchus labrax）, sharing 67.8% amino acid identity. In healthy large yellow croaker, Lc IL-1β transcription was detected in all tested tissues, with the highest level found in the head kidney. Upon Vibrio alginolyticus infection, Lc IL-1β transcription in all tested tissues was significantly upregulated. Intraperitoneal injection of recombinant Lc IL-1β（r Lc IL-1β） improved the survival rate and reduced the tissue bacterial load after V. alginolyticus infection. In addition, r Lc IL-1β induced monocytes/macrophages（MO/MΦ） chemotaxis and increased phagocytosis and bactericidal activity in vitro. These results suggest that Lc IL-1β plays an important role in the large yellow croaker immune response against V. alginolyticus.