Molecular characterization of an IL-1β gene from the large yellow croaker(Larimichthys crocea) and its effect on fish defense against Vibrio alginolyticus infection
Molecular characterization of an IL-1β gene from the large yellow croaker(Larimichthys crocea) and its effect on fish defense against Vibrio alginolyticus infection作者机构:Laboratory of Biochemistry and Molecular Biology School of Manne Sciences Ningbo University Ningbo 315211 China Donghai Sea Collaborative Innovation Center for Industrial Upgrading Mariculture Ningbo University Ningbo 315211 China
出 版 物:《Zoological Research》 (动物学研究(英文))
年 卷 期:2015年第36卷第3期
页 面:133-141页
核心收录:
学科分类:1002[医学-临床医学] 0906[农学-兽医学] 09[农学]
基 金:supported by the National 863 Project(2012AA10A403) the Natural Science Foundation of Ningbo City of China(2014A610187) the Scientific Research Foundation of Graduate School of Ningbo University(G14041)
主 题:Interleukin 1β Large yellow croaker Survival rate Vibrio alginolyticus Monocytes/macrophages
摘 要:Interleukin 1β(IL-1β), the first interleukin to be characterized, plays a key role in regulating the immune response. In this study, we determined the c DNA and genomic DNA sequences of the IL-1β gene from the large yellow croaker, Larimichthys crocea. Phylogenetic analysis indicated that the IL-1β(Lc IL-1β) gene was most closely related to that of European seabass(Dicentrarchus labrax), sharing 67.8% amino acid identity. In healthy large yellow croaker, Lc IL-1β transcription was detected in all tested tissues, with the highest level found in the head kidney. Upon Vibrio alginolyticus infection, Lc IL-1β transcription in all tested tissues was significantly upregulated. Intraperitoneal injection of recombinant Lc IL-1β(r Lc IL-1β) improved the survival rate and reduced the tissue bacterial load after V. alginolyticus infection. In addition, r Lc IL-1β induced monocytes/macrophages(MO/MΦ) chemotaxis and increased phagocytosis and bactericidal activity in vitro. These results suggest that Lc IL-1β plays an important role in the large yellow croaker immune response against V. alginolyticus.