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Single-cell profiling reveals Müller glia coordinate retinal intercellular communication during light/dark adaptation via thyroid hormone signaling

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蛋白质与细胞:英文版 2023年第8期14卷 603-617页

作者： Min Wei Yanping Sun Shouzhen Li Yunuo Chen Longfei Li Minghao Fang Ronghua Shi Dali Tong Jutao Chen Yuqian Ma Kun Qu Mei Zhang Tian Xue Division of Life Sciences and Medicine Department of OphthalmologyThe First Affiliated Hospital of USTCUniversity of Science and Technology of ChinaHefei 230026China Hefei National Research Center for Physical Sciences at the Microscale Neurodegenerative Disorder Research CenterCAS Key Laboratory of Brain Function and DiseaseUniversity of Science and Technology of ChinaHefei 230026China Division of Life Sciences and Medicine School of Life SciencesUniversity of Science and Technology of ChinaHefei 230026China Center for Excellence in Brain Science and Intelligence Technology Chinese Academy of SciencesShanghai 200031China Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijing 100864China

Light adaptation enables the vertebrate visual system to operate over a wide range of ambient *** of phototransduction in photoreceptors is considered a major mechanism underlying light ***,various types of neurons and glial cells exist in the retina,and whether and how all retinal cells interact to adapt to light/dark conditions at the cellular and molecular levels requires systematic ***,we utilized single-cell RNA sequencing to dissect retinal cell-type-specific transcriptomes during light/dark adaptation in *** results demonstrated that,in addition to photoreceptors,other retinal cell types also showed dynamic molecular changes and specifically enriched signaling pathways under light/dark ***,Müller glial cells(MGs)were identified as hub cells for intercellular interactions,displaying complex cell‒cell communication with other retinal ***,light increased the transcription of the deiodinase Dio2 in MGs,which converted thyroxine(T4)to active triiodothyronine(T3).Subsequently,light increased T3 levels and regulated mitochondrial respiration in retinal cells in response to light *** cones specifically express the thyroid hormone receptor Thrb,they responded to the increase in T3 by adjusting light *** of the expression of Dio2 specifically in MGs decreased the light responsive ability of *** results suggest that retinal cells display global transcriptional changes under light/dark adaptation and that MGs coordinate intercellular communication during light/dark adaptation via thyroid hormone signaling.

关键词： single cell Müller glial cells intercellular communication light/dark adaptation thyroid hormone signaling

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Influence of (-)-epigallocatechin-3-gallate on the expression of connexin43 and gap junction intercellular communication of the bladder cancer cell lines BIU-87 in vitro

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The Chinese-German Journal of Clinical Oncology 2011年第12期10卷 716-720页

作者： Zhengguo Cao Chao Tian Maolin Jiang Kui Wu Xiaojian Zhong Jianxin Li Yuefu Han Department of Urology The Affiliated Yuebei People＇s Hospital of Shantou University Medical College Shaoguang 512026 China Department of Urology Anhui Provincial Hospital Affiliated to Anhui Medical College Hefei 230001 China

Objective： The aim of our study was to investigate the effects of （-）-epigallocatechin-3-gallate （EGCG, the major phytochemistry component in green tea） on the expression of connexin43 （Cx43） gene and detect the intercellular communication of the human bladder cancer cell lines BIU-87, and explore its possible mechanisms of prevention and cure for the bladder tumor. Methods： The methyl thiazolyl tetrazolium and Annexin-V/PI double-labeled flow cytometry methods were used to observe the growth inhibitory rate （IR） and apoptosis rate （AR） of BlU-87 cells treated by EGCG at different concentrations （0, 5, 10 and 20 mg/L）, respectively. The reverse transcription-polymerase chain reaction （RT-PCR） and Western Blotting analysis were employed to detect the relative expression levels of the Cx43 mRNA and its protein. The scrape-loading fluorescence dye transfer method was used to assess the gap junction intercellular communication （GJIC） under fluorescence microscope. Results： EGCG at concentrations （10 and 20 mg/L） both could significantly inhibit the proliferation and induce the apoptosis of BIU-87 cells. The IR and AR were （15.67 ± 1.15）%, （18.33 ± 1.53）% and （42.00 ± 4.34）%, （27.33 ± 3.21）%, respectively. And compared with the control groups of 0 mg/L and 5 mg/L （P 〈 0.05）, EGCG could significantly up-regulate the expression of Cx43 mRNA and its protein and enhance the function of BIU-87 cells. The effects had the significant correlation with the dose-dependent of EGCG. Conclusion： EGCG （10, 20 mg/L） could effectively up-regulate Cx43 expression and enhance the GJIC of BlU-87 cells. The results may indicate the effects of EGCG inducing bladder tumor cells apoptosis and inhibiting its growth which provides the experimental evidence for further demonstrating the mechanism of chemical prevention and cure for the bladder tumor by EGCG.

关键词： (-)-epigallocatechin-3-gallate bladder tumor connexin43 intercellular communication

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Study of (-)-epigallocatechin-3-gallate on the expression of connexin43 and gap junction intercellular communication of the bladder cancer cell lines BIU-87 in vitro

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Study of (-)-epigallocatechin-3-gallate on the expression of...

Identification of immune feature genes and intercellular profiles in diabetic cardiomyopathy

第十五届全国泌尿外科学术会议

作者： CAO Zheng-guo, SUN You-wen, ZHU Yu-ping, SHU Hong, WU Bin, DONG Xiao-cheng (Department of Urology, Anhui Provincial Hospital Affiliated to Anhui Medical College 230001)

Objective To assess the current level of prostate cancer awareness and attitude in city living men in China and to raise improvement suggestion. Methods In October, 2007, information was collected from city living men asked to fill a prostate cancer awareness questionnaire in 17 Chinese cities to survey the aspects of demographic information, risk factors for prostate cancer, awareness of the symptoms,

关键词： (-)-epigallocatechin-3-gallate bladder neoplasm connexin43 intercellular communication

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World Journal of Diabetes 2024年第10期15卷 2093-2110页

作者： Ze-Qun Zheng Di-Hui Cai Yong-Fei Song Ningbo Institute of Innovation for Combined Medicine and Engineering The Affiliated Lihuili Hospital of Ningbo UniversityNingbo 315040Zhejiang ProvinceChina Department of Cardiology Clinical Research CenterShantou University Medical CollegeShantou 515041Guangdong ProvinceChina

BACKGROUND Diabetic cardiomyopathy(DCM)is a multifaceted cardiovascular disorder in which immune dysregulation plays a pivotal *** immunological molecular mechanisms underlying DCM are poorly *** To examine the immunological molecular mechanisms of DCM and construct diagnostic and prognostic models of DCM based on immune feature genes(IFGs).METHODS Weighted gene co-expression network analysis along with machine learning methods were employed to pinpoint IFGs within bulk RNA sequencing(RNA-seq)***-sample gene set enrichment analysis(ssGSEA)facilitated the analysis of immune cell *** and prognostic models for these IFGs were developed and assessed in a validation *** expression in the DCM cell model was confirmed through real time-quantitative polymerase chain reaction and western blotting ***,single-cell RNA-seq data provided deeper insights into cellular profiles and *** The overlap between 69 differentially expressed genes in the DCM-associated module and 2483 immune genes yielded 7 differentially expressed immune-related *** IFGs showed good diagnostic and prognostic values in the validation cohort:Proenkephalin(Penk)and retinol binding protein 7(Rbp7),which were highly expressed,and glucagon receptor and inhibin subunit alpha,which were expressed at low levels in DCM patients(all area under the curves>0.9).SsGSEA revealed that IFG-related immune cell infiltration primarily involved type 2 T helper *** expression of Penk(Pcommunication analysis revealed abnormal cellular phenotype transformation and signaling communication in DCM,especially between mesenchymal cells and *** The present study identified Penk and Rbp7 as potential DCM biomarkers,and aberrant mesenchymal-immune cell phenotyp

关键词： Diabetic cardiomyopathy Immune feature genes Proenkephalin Retinol binding protein 7 Immune cell infiltration intercellular communication

Single-cell manipulation by two-dimensional micropatterning

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Journal of Innovative Optical Health Sciences 2024年第1期17卷 45-59页

作者： Xuehe Ma Haimei Zhang Shiyu Deng Qiushuo Sun Qingsong Hu Yuhang Pan Fen Hu Imshik Lee Fulin Xing Leiting Pan The Key Laboratory of Weak-Light Nonlinear Photonics of Education Ministry School of Physics and TEDA Institute of Applied Physics Nankai University Tianjin 300071P.R.China State Key Laboratory of Medicinal Chemical Biology Frontiers Science Center for Cell Responses College of Life Sciences Nankai University Tianjin 300071P.R.China Collaborative Innovation Center of Extreme Optics Shanxi University TaiyuanShanxi 030006P.R.China Shenzhen Research Institute of Nankai University Shenzhen Guangdong 518083P.R.China

Cells are highly sensitive to their geometrical and mechanical microenvironment that directly regulate cell shape,cytoskeleton and organelle,as well as the nucleus morphology and genetic *** emerging two-dimensional micropatterning techniques offer powerful tools to construct controllable and well-organized microenvironment for single-cell level investigations with qualitative analysis,cellular standardization,and in vivo environment ***,we provide an overview of the basic principle and characteristics of the two most widely-used micropatterning techniques,including photolithographic micropatterning and soft lithography ***,we summarize the application of micropatterning technique in controlling cytoskeleton,cell migration,nucleus and gene expression,as well as intercellular communication.

关键词： Two-dimensional micropatterning cytoskeleton cell migration extracellular matrix intercellular communication gene expression

Single-cell analysis of cellular heterogeneity and interactions in the ischemia-reperfusion injured mouse intestine

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药物分析学报:英文版 2023年第7期13卷 760-775页

作者： Lianhong Yin Meng Gao Lina Xu Yan Qi Lan Han Jinyong Peng College of Pharmacy Dalian Medical UniversityDalianLiaoning116044China School of Pharmacy Anhui University of Chinese MedicineHefei230012China

Nine major cell populations among 46,716 cells were identified in mouse intestinal ischemia‒reperfusion(II/R)injury by single-cell RNA *** enterocyte cells,11 subclusters were found,in which enterocyte cluster 1(EC1),enterocyte cluster 3(EC3),and enterocyte cluster 8(EC8)were newly discovered cells in ischemia 45 min/reperfusion 720 min(I 45 min/R 720 min)***1 and EC3 played roles in digestion and absorption,and EC8 played a role in cell *** TA cells,after ischemia 45 min/reperfusion 90 min(I 45 min/R 90 min),many TA cells at the stage of proliferation were *** Paneth cells,Paneth cluster 3 was observed in the resting state of normal *** I 45 min/R 90 min,three new subsets were found,in which Paneth cluster 1 had good antigen presentation *** main functions of goblet cells were to synthesize and secrete mucus,and a novel subcluster(goblet cluster 5)with highly proliferative ability was discovered in I 45 min/R 90 min *** a major part of immune system,the changes in T cells with important roles were ***,enterocyte cells secreted Guca2b to interact with Gucy2c receptor on the membranes of stem cells,TA cells,Paneth cells,and goblet cells to elicit intercellular *** marker known as glutathione S-transferase mu 3(GSTM3)affected intestinal mucosal barrier function by adjusting mitogen-activated protein kinases(MAPK)signaling during II/R *** data on the heterogeneity of intestinal cells,cellular communication and the mechanism of GSTM3 provide a cellular basis for treating II/R injury.

关键词： Single-cell RNA-sequencing Intestinal cells Cell heterogeneity Enterocyte cells intercellular communication

Cisplatin-induced premature senescence with concomitant reduction of gap junctions in human fibroblasts

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Cell Research 2004年第1期14卷 60-66页

作者： Wei ZHAO, Zhong Xiang LIN, Zhi Qian ZHANG Peking University School of Oncology, and Beijing Institute for Cancer Research, Beijing 100034, China. Lin ZX Peking Univ Sch Oncol Beijing 100034 Peoples R China

To examine the role of gap junctions in cell senescence, the changes of gap junctions in cisplatin-induced premature senescence of primary cultured fibroblasts were studied and compared with the replicative senescent human fibroblasts. Dye transfer assay for gap junction function and immunofluorescent staining for connexin 43 protein distribution were done respectively. Furthermore, cytofluorimetry and DAPI fluorescence staining were performed for cell cycle and apoptosis analysis, p53 gene expression level was detected with indirect immunofluorescence. We found that cisplatin (10 mM) treatment could block cell growth cycle at G1 and induced premature senescence. The premature senescence changes included high frequency of apoptosis, elevation of p53 expression, loss of membranous gap junctions and reduction of dye-transfer capacity. These changes were comparable to the changes of replicative senescence of human fibroblasts. It was also concluded that cisplatin could induce premature senescence concomita

关键词： cisplatin premature senescence gap junction intercellular communication connexin 43 fibroblasts.

Extracellular microRNAs from the epididymis as potential mediators of cell-to-cell communication

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Asian Journal of Andrology 2015年第5期17卷 730-736,I0006-I0007页

作者： Clemence Belleannee Department of Obstetrics Gynecology and Reproduction Faculty of Medicine Universite Laval Reproduction Mother and Youth Health Division Research Center CHU de Quebec Quebec Canada G1V 4G2

Ribonucleic acid （RNA） was previously thought to remain inside cells as an intermediate between genes and proteins during translation. However, it is now estimated that 98% of the mammalian genomic output is transcribed as noncoding RNAs, which are involved in diverse gene expression regulatory mechanisms and can be transferred from one cell to another through extracellular communication. For instance, microRNAs are 22-nucleotide-long noncoding RNAs that are generated by endonuclease cleavage of precursors inside the cells and are secreted as extracellular microRNAs to regulate target cell posttranscriptional gene expression via RNA interference. We and others have shown that different populations of microRNAs are expressed in distinct regions of the human epididymis and regulate the expression of target genes that are involved in the control of male fertility as indicated by knock-out mouse models. Importantly, some microRNAs, including the microRNA-888 （miR-888） cluster that is exclusively expressed in the reproductive system of human and nonhuman primates, are released in the sperm-surrounding fluid in the epididymis via extracellular vesicles, the so-called epididymosomes. In addition to interacting with the membrane of maturing spermatozoa, these extracellular vesicles containing microRNAs communicate with epithelial cells located downstream from their release site, suggesting a role in the luminal exocrine control of epididymal functions. Apart from their potential roles as mediators of intercellular communication within the epididymis, these extracellular microRNAs are potent molecular targets for the noninvasive diagnosis of male infertility.

关键词： Dicer epididymis exocrine factors extracellular vesicles intercellular communication microRNAs paracrine regulation posttranscriptional gene regulation seminal plasma spermatozoa

The effect of exosomes in transferring TET signaling alterations

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BIOCELL 2022年第3期46卷 579-581页

作者： SERGIU PASCA ANCUTA JURJ Department of Hematology Iuliu Hatieganu University of Medicine and PharmacyCluj-Napoca400012Romania Research Center for Functional Genomics Biomedicine and Translational MedicineIuliu Hatieganu University of Medicine and PharmacyCluj-Napoca400337Romania

Ten eleven translocation(TET)enzymes are composed of three representatives:TET1/2/3 which are involved in the hydroxymethylation of methylated *** of the wide array of processes that are governed by these epigenetic marks,there have been a wide range of clinical effects associated with TET *** though many research groups have focused on analyzing the effect of TET alterations within certain cells,few have taken into consideration the effect of TET in the context of intercellular *** important entity through which intercellular communication occurs is represented by ***,in the current viewpoint we discussed the direct transfer of TET by exosomes,its alterations in the cell targeted by exosomes and the effect of TET alterations on exosome secretion.

关键词： TET Exosomes Immunity intercellular communication