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in vivo antioxidant activity of rabbiteye blueberry(Vaccinium ashei cv.'Brightwell')anthocyanin extracts

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2023年第7期24卷 602-616页

作者： Jing WANG Xingyu ZHAO Jiawei ZHENG Daniela D.HERRERA-BALANDRANO Xiaoxiao ZHANG Wuyang HUANG Zhongquan SUI College of Chemical Engineering Nanjing Forestry UniversityNanjing 210037China Institute of Agro-Product Processing Jiangsu Academy of Agricultural SciencesNanjing 210014China School of Food and Biological Engineering Jiangsu UniversityZhenjiang 212013China School of Life Sciences Nantong UniversityNantong 226007China Department of Food Science&Engineering School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China

Blueberries are rich in phenolic compounds including anthocyanins which are closely related to biological health *** purpose of this study was to investigate the antioxidant activity of blueberry anthocyanins extracted from'Brightwell'rabbiteye blueberries in *** one week of adaptation,C57BL/6J healthy male mice were divided into different groups that were administered with 100,400,or 800 mg/kg blueberry anthocyanin extract(BAE),and sacrificed at different time points(0.1,0.5,1,2,4,8,or 12 h).The plasma,eyeball,intestine,liver,and adipose tissues were collected to compare their antioxidant activity,including total antioxidant capacity(T-AOC),superoxide dismutase(SOD)activity and glutathione-peroxidase(GSH-PX/GPX)content,and the oxidative stress marker malondialdehyde(MDA)*** results showed that blueberry anthocyanins had positive concentration-dependent antioxidant activity in *** greater the concentration of BAE,the higher the T-AOC value,but the lower the MDA *** enzyme activity of SOD,the content of GSH-PX,and messenger RNA(mRNA)levels of Cu,Zn-SOD,Mn-SOD,and GPX all confirmed that BAE played an antioxidant role after digestion in mice by improving their antioxidant *** in vivo antioxidant activity of BAE indicated that blueberry anthocyanins could be developed into functional foods or nutraceuticals with the aim of preventing or treating oxidative stress-related diseases.

关键词： Blueberry anthocyanin in vivo antioxidant activity Superoxide dismutase(SOD) Glutathione-peroxidase(GSH-PX/GPX) Malondialdehyde(MDA)

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