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Capsid Proteins from PCV2a Genotype Confer Greater Protection against a PCV2b Strain than those from PCV2b Genotype in Pigs:Infers the Reason of PCV2b Strains Becoming More Predominant than PCV2a Strains

Capsid Proteins from PCV2a Genotype Confer Greater Protectio...

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第十届全国免疫学学术大会

作者： Liping Huang Yiping Wang Yanwu Wei Dongjie Chen Dan Liu Wenjuan Du Deli Xia Li Feng Changming Liu Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences

Background:Two major porcine circovirus type 2(PCV2)genotypes,PCV2a and PCV2b,are ***2a was predominant in the global pig population until 2000 while PCV2b became predominant from 2003 ***:The aim of this study was to analyze the immune protection conferred by two PCV2a and two PCV2b capsid proteins(Caps)in pigs challenged with a mutant PCV2b/YJ(m PCV2b/YJ)***:In this study,PCV2a/LG-Cap,PCV2a/CL-Cap,PCV2b/JF-Cap,and mutant PCV2b/YJ(m PCV2b/YJ)-Cap were expressed in a baculovirus system,and immune protection was investigated in pigs challenged with the m PCV2b/YJ ***:Pigs vaccinated with PCV2a/LG-Cap and PCV2a/CL-Cap elicited significantly higher levels of PCV2-specific antibodies and neutralizing antibodies compared with PCV2b/JF-Cap and m PCV2b/*** a m PCV2b/YJ challenge,no viremia was detected in the PCV2a/LG-Cap and PCV2a/CL-Cap groups,while viremias were found in 20%and 40%of the pigs in the PCV2b/JF-Cap and m PCV2b/YJ-Cap groups,*** loads in the inguinal lymph nodes of pigs from the PCV2b/JF-Cap and m PCV2b/YJ-Cap groups were significantly higher than those in the PCV2a/LG-Cap and PCV2a/CL-Cap groups,but significantly lower than that of the challenge control ***,PCV2antigens were not detected in the inguinal lymph nodes of pigs from commercial vaccine groups,the PCV2a/LG-Cap and PCV2a/CL-Cap groups,but were found in the challenge control(100%,5/5),PCV2b/JF-Cap(20%,1/5),and m PCV2b/YJ-Cap(20%,1/5)***:These findings suggest that m PCV2b/YJ-Cap and PCV2b/JF-Cap were less immunogenic than PCV2a/LG-Cap and PCV2a/CL *** speculate that a genotypic shift from PCV2a to PCV2b might be the result of the majority of PCV2a strains being more immunogenic than the majority of PCV2b strains.

关键词： Porcine circovirus type 2 PCV2a and PCV2b genotypes immunogenicity

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The Effect of Recombinant Human Parathyroid Hormone 1-84 on Binding Antibody Level in Sprague-dawley Rats and Rhesus Monkeys

The Effect of Recombinant Human Parathyroid Hormone 1-84 on ...

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The 12th Asian Pacific Confederation of Chemical Engineering Congress

作者： Liu Qinghai~a Wu Hao~b Zhang Xiao-Dong~c Qin Ying~a Tao Xin-Yi~a Ma Xing-Yuan~a Wei Dong-Zhi~a Yang Sheng-Li~d Yuan Bo-Jun~e a New World Institute of Biotechnology State Key Laboratory of Bioreactor Engineering East China University of Science and Technology 200237 Shanghai China b Center for Drug Evaluation Shanghai Municipal Food and Drug Administration 200021 Shanghai China c Genter for New Drug Evaluation The Second Military Medical University 200433 Shanghai China d Shanghai Research Genter of Biotechnology Chinese Academy of Sciences 200233 Shanghai China

＜正＞We studied the effect of Recombinant human parathyroid hormone 1-84（rhPTH 1-84）on the immunogenicity in Sprague-Dawley rats and Rhesus *** Rats were divided to re- ceive once daily subcutaneous injection of 20... 详细信息

关键词： rhPTH 1-84 immunogenicity binding antibodies sprague-dawley rats rhesus monkeys

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Effect of Intracellular Uptake of Nanoparticle-encapsulated Trehalose on the Hemocompatibility of Allogeneic Valves in the VS83 Vitrification Protocol

Effect of Intracellular Uptake of Nanoparticle-encapsulated ...

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作者： Balamurugan Vasudevan 青岛大学

学位级别：硕士

Research Background:The use of human biological valves referred to as allograft heart valves（AHV）is shown to have numerous improvements above conventional xenografts,significantly concerning hemodynamic reaction and *** allogeneic heart valves preservation necessitates meticulous attention to ensure that the cell integrity is preserved prior to clinical ***,vitrification protocols were reported to preserve the matrix integrity of allograft valves and concomitantly result in low tissue *** has been shown to be involved in retaining cell viability after thawing in IFC *** researches showed that nanoparticle encapsulated trehalose resulted in cryopreservation of mammalian cells with high viability and unblemished *** practice,they are using a nanoparticle to encapsulate Trehalose and using it in the VS83 protocol results in minor ice *** of cryopreserved heart valve has been a hurdle demanding the recipient to live on anticoagulants for their entire *** and inflammation significantly hinder the valve grafting due to *** these issues can be sorted out by tissue engineering which will take a long time to achieve ***’s effect on hemocompatibility must be evaluated because it has been shown to be excellent at maintaining cell ***:This study aims to see whether Trehalose affects the allogeneic valves hemocompatibility in the VS83 procedure,precisely if the disaccharide will induce an immune reaction and decreases immune response after *** addition,the research seeks to assess if nanoparticle-encapsulated Trehalose increases cell viability in IFC cardiovascular preservation techniques and maintains the extracellular matrix *** primary theory is that Trehalose increases cell viability by vitrifying allogeneic heart valves and safeguards ECM *** second theory states that the absorption of T

关键词： Ice-FreeCryopreservation Homograftvalve Apatite Trehalose Nanoparticle immunogenicity VS83

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肿瘤微环境的免疫原性对局部放疗引发远隔效应的影响及思考（英文）

肿瘤微环境的免疫原性对局部放疗引发远隔效应的影响及思考（英文...

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第十四届全国免疫学学术大会

作者：赖金枝朱艳阳周琳琳胡莉陈玲张秋玉福建医科大学免疫治疗研究院福建医科大学附属第二医院

Although abscopal tumor regression remained a rare *** generated two distinct tumor models stratified as highly(MC-38 and E.G7-OVA) or poorly(LL/2 and B16-F10) immunogenic *** established tumor models were treated with sham-radiation,a single-dose of 15 Gy,or 3 fractions of 5 Gy in three consecutive days,*** results demonstrated local radiation reshaped tumor microenvironment of irradiated and non-irradiated distant tumors by increasing CD8 T-cell infiltration and reducing the accumulation of suppressive immune cells in highly immunogenic ***,no significant alteration was detected in non-irradiated distant tumor microenvironment after radiation in poorly immunogenic *** addition,local radiation alone was insufficient to elicit abscopal effects in poorly immunogenic *** findings indicated that tumor immunogenicity was the dominant characteristic which could predict abscopal effect of radiotherapy.

关键词： radiation abscopal effect immunogenicity tumor microenvironment

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Frontier of therapeutic antibody discovery:The challenges and how to face them

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World Journal of Biological Chemistry 2012年第12期3卷 187-196页

作者： Zhi-Jian Lu Su-Jun Deng Da-Gang Huang Yun He Ming Lei Li Zhou Pei Jin China Novartis Institutes for Biomedical Research Zhangjiang Hi-Tech Park Novartis Institutes for Biomedical Research Inc.250 Massachusetts Avenue

Therapeutic monoclonal antibodies have become an important class of modern *** established technologies for therapeutic antibody discovery such as humanization of mouse antibodies,phage display of human antibody libraries and transgenic animals harboring human IgG genes have been practiced successfully so far,and many incremental improvements are being made *** methodologies are responsible for currently marketed therapeutic antibodies and for the biopharma industry pipeline which are concentrated on only a few dozen targets.A key challenge for wider application of biotherapeutic approaches is the paucity of truly validated targets for biotherapeutic *** efforts to expand the target space include taking the pathway approach to study the disease *** many new targets are multi-spanning and multimeric membrane proteins there is a need to develop more effective methods to generate antibodies against these difficult *** pharmaceutical properties of therapeutic antibodies are an active area for study concentrating on biophysical characteristics such as thermal stability and aggregation *** immunogenicity of biotherapeutics in humans is a very complex issue and there are no truly predictive animal models to rely *** in silico and T-cell response approaches identify the potential for immunogenicity;however,one needs contingency plans for emergence of antiproduct antibody response for clinical trials.

关键词： Antibody Biotherapeutics Biophysical property Bispecific Humanization immunogenicity Transgenic rodent Phage display Yeast

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