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痰热清注射液对脂多糖诱导大鼠气道粘液高分泌（英文）

痰热清注射液对脂多糖诱导大鼠气道粘液高分泌（英文）

引用

贵州省中西医结合学会呼吸专业委员会2015年年会

作者： Shou-jin Dong Yun-qing Zhong Wen-ting Lu Kun Yang Mao-juan Liu Xiao-ling Xiong Bing Mao Department of Integrated Traditional Chinese and Western Medicine West China HospitalWest China Medical SchoolSichuan University Department of Pneumology Chengdu First People's HospitalChengdu Integrated Traditional Chinese and Western Medicine Hospital Department of Pneumology The First Affiliated Hospital of Guangxi Traditional Chinese Medical University Department of geratology Sichuan Hospital of Integrated Traditional Chinese and Western Medicine Department of geratology Chengdu University of Traditional Chinese Medicine

Background:Tanreqing injection,a Chinese herbal formulation comprising Radix Scutellariae,Fructus Forsythiae,Flos Lonicerae,Antelope horn,and Bear bile powder,has been used to treat bronchitis and pneumonia for many years in ***,its anti-mucus-hypersecretion mechanism has yet not been fully *** aim to assess the effect and dose-response relationships of Tanreqing injection on lipopolysacchaide(LPS) induced airway mucus hypersecretion in *** and methods:Forty-eight male rats were randomly divided into four groups(12 per group).A rat model of airway mucus hypersecretion was generated with *** injection was given by intratracheal instillation,and bronchoalveolar lavage fluid(BALF)from the right lung was *** total protein was determined by bicinchoninic acid disodium assay(BCA).Muc5 ac was measured using enzyme linked immunosorbent assay(ELISA) and *** expression of muc5ac mRNA was detected by real-time polyermase chain reaction(RT-PCR).The middle lobe of the right lung was stained with alcian blue-periodic acid sthiff(AB-PAS) and positive staining relative shading area ***:LPS caused airway mucus hypersecretion,The LPS-induced airway mucus hypersecretion increased beginning at 24 hr,and peaked at96 *** injection could inhibit airway mucus hypersecretion,and suppress the expression of muc5 ac ***:Tanreqing injection inhibits airway mucus hypersecretion in a certain dose-dependent trend.

关键词： Tanreqing injection iipopolysaccharides airway mucus hypersecretion dose-response relationship

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