痰热清注射液对脂多糖诱导大鼠气道粘液高分泌（英文）
作者单位：Department of Integrated Traditional Chinese and Western MedicineWest China HospitalWest China Medical SchoolSichuan University Department of PneumologyChengdu First People's HospitalChengdu Integrated Traditional Chinese and Western Medicine Hospital Department of PneumologyThe First Affiliated Hospital of Guangxi Traditional Chinese Medical University Department of geratologySichuan Hospital of Integrated Traditional Chinese and Western Medicine Department of geratologyChengdu University of Traditional Chinese Medicine
会议名称：《贵州省中西医结合学会呼吸专业委员会2015年年会》
会议日期：2015年
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
基 金：supported by the Program for Innovative Research at the West China School of Medicine Sichuan University and Sichuan Technology Department(No.2012SZ0288)
关 键 词：Tanreqing injection Iipopolysaccharides airway mucus hypersecretion dose-response relationship
摘 要：Background:Tanreqing injection,a Chinese herbal formulation comprising Radix Scutellariae,Fructus Forsythiae,Flos Lonicerae,Antelope horn,and Bear bile powder,has been used to treat bronchitis and pneumonia for many years in ***,its anti-mucus-hypersecretion mechanism has yet not been fully *** aim to assess the effect and dose-response relationships of Tanreqing injection on lipopolysacchaide(LPS) induced airway mucus hypersecretion in *** and methods:Forty-eight male rats were randomly divided into four groups(12 per group).A rat model of airway mucus hypersecretion was generated with *** injection was given by intratracheal instillation,and bronchoalveolar lavage fluid(BALF)from the right lung was *** total protein was determined by bicinchoninic acid disodium assay(BCA).Muc5 ac was measured using enzyme linked immunosorbent assay(ELISA) and *** expression of muc5ac mRNA was detected by real-time polyermase chain reaction(RT-PCR).The middle lobe of the right lung was stained with alcian blue-periodic acid sthiff(AB-PAS) and positive staining relative shading area ***:LPS caused airway mucus hypersecretion,The LPS-induced airway mucus hypersecretion increased beginning at 24 hr,and peaked at96 *** injection could inhibit airway mucus hypersecretion,and suppress the expression of muc5 ac ***:Tanreqing injection inhibits airway mucus hypersecretion in a certain dose-dependent trend.