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PEDV ORF3对IFN-β及isgs转录水平的抑制效应

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黑龙江八一农垦大学学报 2018年第5期30卷 82-88页

作者：程丽鑫姚丽莉邹德华郑亮李兴志张雅婷徐嘉欣武雪宁王显赫张华曹宏伟黑龙江八一农垦大学生命科学技术学院大庆163319

探究PEDV ORF3蛋白对I型干扰素及其信号分子转录水平的影响。构建PEDV ORF3蛋白真核表达载体pcDNA3.0-FlagORF3,将重组载体以时间梯度和剂量梯度转染至293T细胞。通过RT-PCR检测ORF3对IFN-β、ISG54、ISG56转录水平的影响。结果显示,随... 详细信息

探究PEDV ORF3蛋白对I型干扰素及其信号分子转录水平的影响。构建PEDV ORF3蛋白真核表达载体pcDNA3.0-FlagORF3,将重组载体以时间梯度和剂量梯度转染至293T细胞。通过RT-PCR检测ORF3对IFN-β、ISG54、ISG56转录水平的影响。结果显示,随着重组载体转染剂量的增加,IFN-βmRNA转录水平逐渐降低;将重组载体以时间梯度转染后,IFN-βmRNA转录水平在转染24 h后开始降低,ISG54、ISG56 mRNA转录水平在转染12 h后开始降低;并且PEDV ORF3蛋白明显抑制poly(I:C)诱导的IFN-βmRNA转录。以上结果表明PEDV ORF3蛋白可抑制IFN-β、ISG54、ISG56 mRNA转录,具有拮抗I型干扰素应答的功能。

关键词： PEDV ORF3 IFN-β isgs

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Relevance of placental type I interferon beta regulation for pregnancy success

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Cellular & Molecular Immunology 2018年第12期15卷 1010-1026页

作者： Ja-Young Kwon Paulomi Aldo Yuan You Jiahui Ding Karen Racicot Xiaoyan Dong John Murphy Guy Glukshtad Michelle Silasi Jian Peng Li Wen Vikki MAbrahams Roberto Romero Gil Mor Division of Reproductive Sciences Department of ObstetricsGynecology and Reproductive SciencesYale School of MedicineNew HavenCTUSA Department of Obstetrics and Gynecology Institute of Women’s Life Medical ScienceYonsei University College of MedicineSeoulKorea Department of Obstetrics Gynecology&Reproductive BiologyCollege of Human MedicineMichigan State University400 Monroe Avenue NEGrand RapidsMI 49503USA Department of Pulmonary Shanghai Children’s HospitalShanghai Jiaotong University School of MedicineShanghaiChina Division of Endocrinology Department of Internal MedicineYale School of MedicineNew HavenCTUSA Perinatal Research Branch NICHD/NIHDetroitMIUSA

Pregnancy is a unique immunologic and microbial condition that requires an adequate level of awareness to provide a fast and protective response against pathogens as well as to maintain a state of tolerance to paternal *** of inflammatory pathways in the placenta triggered by pathogens is one of the main factors responsible for pregnancy *** I IFNs are key molecules modulating immune responses at the level of the placenta and are crucial for protection of the pregnancy via their antiviral and immune modulatory *** this study,we elucidate the mechanisms controlling the basal expression of IFNβand its negative *** in vitro and in vivo animal models,we found that TLR signaling maintains basal IFNβlevels through the TLR4-MyD88-independent TBK/IRF3 signaling *** describe the role of the TAM receptor Axl in the regulation of IFNβfunction in human and mouse trophoblast *** absence of TAM receptors in vivo is associated with fetal demise due to dysregulation of IFNβexpression and its pro-apoptotic downstream ***,our data describe a feedback signaling pathway controlling the expression and function of IFNβin the trophoblast that is essential for an effective response during viral and microbial infections.

关键词： trophoblast immune regulation cytokine TAM receptors type I interferon Interferon beta isgs

FoxJ1 inhibits African swine fever virus replication and viral S273R protein decreases the expression of FoxJ1 to impair its antiviral effect

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Virologica Sinica 2022年第3期37卷 445-454页

作者： Caina Ma Shasha Li Fan Yang Weijun Cao Huisheng Liu Tao Feng Keshan Zhang Zixiang Zhu Xiangtao Liu Yonghao Hu Haixue Zheng College of Veterinary Medicine Gansu Agricultural UniversityLanzhou730070China State Key Laboratory of Veterinary Etiological Biology National Foot and Mouth Diseases Reference LaboratoryKey Laboratory of Animal Virology of Ministry of AgricultureLanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhou730046China

African swine fever(ASF)is a highly pathogenic swine infectious disease that affects domestic pigs and wild boar,which is caused by the African swine fever virus(ASFV).ASF has caused huge economic losses to the pig industry and seriously threatens global food security and livestock *** date,there is no safe and effective commercial vaccine against *** the underlying mechanisms of ASFV-host interplay is critical for developing effective vaccines and drugs against *** the present study,RNA-sequencing,RT-qPCR and Western blotting analysis revealed that the transcriptional and protein levels of the host factor FoxJ1 were significantly down-regulated in primary porcine alveolar macrophages(PAMs)infected by ***-qPCR analysis showed that overexpression of FoxJ1 upregulated the transcription of type I interferon and interferon stimulating genes(isgs)induced by poly(dA:dT).FoxJ1 revealed a function to positively regulate innate immune response,therefore,suppressing the replication of *** addition,Western blotting analysis indicated that FoxJ1 degraded ASFV MGF505-2R and E165R proteins through autophagy ***,RT-qPCR and Western blotting analysis showed that ASFV S273R inhibited the expression of ***,we determined that FoxJ1 plays an antiviral role against ASFV replication,and ASFV protein impairs FoxJ1-mediated antiviral effect by degradation of *** findings provide new insights into the antiviral function of FoxJ1,which might help design antiviral drugs or vaccines against ASFV infection.

关键词： African swine fever virus(ASFV) FoxJ1 isgs MGF505-2R E165R S273R

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IMB-0523 Inhibits Enterovirus 71 Replication by Activating Signal Transducer and Activator of Transcription 3 Signaling to Upregulate Interferon-Stimulated Genes Expression

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Infectious Diseases & Immunity 2023年第1期3卷 13-19页

作者： Huiqiang Wang Miao Ge Yanping Li Yuhuan Li Zhuorong Li CAMS Key Laboratory of Antiviral Drug Research Institute of Medicinal BiotechnologyChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China Beijing Key Laboratory of Antimicrobial Agents Institute of Medicinal BiotechnologyChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China.

Background Hand,foot,and mouth disease caused by enterovirus 71(EV71)infection is prevalent in the Asia-Pacific region in recent ***,no drug is available for the prevention and treatment of EV71 ***-0523,a N-phenylbenzamide derivative,inhibits hepatitis B virus replication by upregulating the expression of *** the present study,the effect of IMB-0523 on EV71 replication and related mechanism were *** The cytotoxicity of IMB-0523 was determined by cell counting *** real-time polymerase chain reaction and Western blot assay were used to detect the effect of IMB-0523 on EV71 replication and related *** effect assay was used to investigate the effect of IMB-0523 on different EV71 strains,coxsackievirus A16,and coxsackieviruses of group *** The results showed that IMB-0523 could dose-dependently inhibit EV71 *** mechanism studies showed that IMB-0523 could activate STAT3 signaling to upregulate the expression of interferon-stimulated genes to play an antiviral *** addition,IMB-0523 inhibited the replication of different EV71 strains,coxsackievirus A16,and coxsackieviruses of group *** IMB-0523 inhibits EV71 replication by activating the STAT3 signaling pathway to upregulate interferon-stimulated gene ***-0523 has broad-spectrum antiviral potential and may be used as a lead compound for the development of broad spectrum antiviral drugs.

关键词： Hand,foot,and mouth disease Enterovirus 71 IMB-0523 isgs STAT3

X-protein Negatively Regulate Type Ⅰ Interferon-mediated Innate Antiviral Responses

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X-protein Negatively Regulate Type Ⅰ Interferon-mediated In...

Expression level of interferon-stimulated genes PKR,OAS1,MX1,and ISG15 in peripheral blood mononuclear cells of COVID-19 patients:A retrospective study

第十三届全国免疫学学术大会

作者： Xin Wang Yuan Zhang Xuetao Cao Peking Union Medical College

The interferon(IFN)-mediated innate antiviral immune response provides a robust first line of defense against invading pathogens, and interferon-stimulated genes(isgs) are essential effectors of *** regulation of isgs is one of contributors to the pathogenesis of abortive antiviral responses and autoimmune ***-transcriptomic regulation plays key roles in diverse biological processes, including defining cell status and affecting cell differentiation.N-methyladenosine(mA) modification is the most abundant internal modification in eukaryotic mRNA and has been discovered as the first example of reversible RNA ***, we found that the mA "reader" X-protein suppresses isgs constitutive expression by promoting translation of the transcription co-repressor *** cooperation with two cofactors, PABP1 and eIF4G2, X-protein binds to the translation initiation region(TIR) of FOXO3 mRNA and promotes FOXO3 *** the YTH and P/Q/N-rich domains of X-protein are required for its binding capacity to FOXO3 ***, METTL3-mediated mA modification was not involved in this ***, X-gene deficiency mice have increased isgs levels and enhanced resistance to several virus *** findings identify X-protein as a negative regulator of antiviral immunity through translational promotion of FOXO3 mRNA under homeostatic conditions, adding insight into the network of RBP-RNA interactions in maintaining host antiviral immune function and preventing inflammatory response.

关键词： innate immunity type Ⅰ interferon isgs X-protein FOXO3

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Journal of Acute Disease 2024年第3期13卷 111-115页

作者： Elham Jafari Maskouni Samaneh Abbasi Elham Mousavi Zahra Najafimemar Ali Mohammad Arabzadeh Mehrdad Farrokhnia Saeedeh Ebrahimi Department of Medical Microbiology(Bacteriology&Virology) Afzalipour Faculty of MedicineKerman University of Medical SciencesKermanIran Abadan University of Medical Sciences AbadanIran Infectious Diseases Research Center Golestan University of Medical SciencesGorganIran Department of Internal Medicine Afzalipour School of MedicineKerman University of Medical SciencesKermanIran

Objective:To explore expression level of interferon-stimulated genes PKR,OAS1,MX1,and ISG15 in peripheral blood mononuclear cells of COVID-19 ***:In this study,changes in the expression of four interferon-stimulated genes(isgs),including PKR,OAS1,MX1,and ISG15,in peripheral blood mononuclear cells of 45 COVID-19 patients with different severities were evaluated by real-time PCR ***:OAS1,MX1,PKR,and ISG15 were differently expressed in COVID-19 patients with different *** results showed that the expression of OAS1,MX1,PKR,and ISG15 genes was significantly(P=0.001)lower in severe ***:Weak and defective IFN response and subsequent disruption of isgs may be associated with COVID-19 severity.

关键词： COVID-19 SARS-CoV-2 Interferon isgs Severe COVID-19 Risk factors Interferon signaling

IFN-α对山羊副流感病毒3型的抗病毒活性

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畜牧兽医学报 2023年第2期54卷 736-743页

作者：孙敏郝飞张纹纹李文良杨蕾蕾毛立程子龙刘茂军江苏省农业科学院兽医研究所/农业部兽用生物制品工程技术重点实验室南京210014 江苏大学生命科学学院镇江212013 江苏大学食品与生物工程学院镇江212013 南京农业大学动物医学院南京210095

旨在探讨原核表达纯化的山羊α干扰素(IFN-α)对山羊副流感病毒3型(CPIV3)的抗病毒活性。通过分析山羊IFN-α的序列特点,比对不同种属IFN-α的同源性,进而构建山羊IFN-α成熟蛋白编码基因(去除信号肽基因序列)的原核表达载体pET-30a-gI... 详细信息

旨在探讨原核表达纯化的山羊α干扰素(IFN-α)对山羊副流感病毒3型(CPIV3)的抗病毒活性。通过分析山羊IFN-α的序列特点,比对不同种属IFN-α的同源性,进而构建山羊IFN-α成熟蛋白编码基因(去除信号肽基因序列)的原核表达载体pET-30a-gIFN-α,将其转化至感受态细胞Rosetta(DE3),IPTG诱导后镍柱及亲和纯化获得山羊IFN-α。利用RT-qPCR测定山羊IFN-α作用于牛肾细胞(Madin-Darby bovine kidney cell,MDBK cell)后6种干扰素刺激基因(interferon-stimulated genes,isgs)的相对表达水平;同时,利用TCID50及Western blot测定其对CPIV3的抗病毒活性。结果表明,原核表达的山羊IFN-α蛋白含量为0.20 mg·mL^(-1),Western blot结果表明表达产物相对分子质量约为20 ku,与预期结果相符。RT-qPCR结果显示,山羊IFN-α孵育MDBK细胞后,可显著刺激RSAD2、STAT1及ISG15等6种isgs基因的转录上调。与对照组细胞相比,山羊IFN-α显著下调MDBK细胞中CPIV3的病毒滴度,Western blot结果显示,山羊IFN-α孵育显著下调CPIV3非结构蛋白C的表达水平。山羊IFN-α对CPIV3在MDBK细胞中的增殖具有显著抑制作用。本研究扩展了I型干扰素的研究范围,为CPIV3等反刍动物疫病的防控提供新的思路。

关键词：山羊IFN-α 原核表达 isgs 山羊副流感病毒3型抗病毒活性

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长链非编码RNA MSTRG.15296.5在流感病毒感染中的作用

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畜牧与兽医 2024年第12期56卷 97-103页

作者：金佳昱陈纳梁冰孟琪邓璐璐曾亦然平继辉南京农业大学动物医学院江苏南京210095

旨在分析H1N1亚型流感病毒A/WSN/1934(WSN)感染A549细胞后差异表达的长链非编码RNA(lncRNA)MSTRG.15296.5作用,为阐明流感病毒致病相关lncRNA的功能及机制提供基础。利用实时荧光定量PCR验证MSTRG.15296.5的表达;通过空斑试验确定MSTRG.... 详细信息

旨在分析H1N1亚型流感病毒A/WSN/1934(WSN)感染A549细胞后差异表达的长链非编码RNA(lncRNA)MSTRG.15296.5作用,为阐明流感病毒致病相关lncRNA的功能及机制提供基础。利用实时荧光定量PCR验证MSTRG.15296.5的表达;通过空斑试验确定MSTRG.15296.5对病毒复制的影响;检测天然免疫相关基因的表达并对MSTRG.15296.5潜在靶基因进行分析。结果:MSTRG.15296.5对WSN的感染具有促进作用,能够下调多个干扰素刺激基因(isgs)的表达。综上,MSTRG.15296.5通过抑制isgs介导的天然免疫信号通路促进流感病毒复制,为宿主和病毒相互作用提供了一定理论依据,为后续防控流感病毒感染提供了新思路。

关键词：长链非编码RNA 甲型流感病毒病毒复制 isgs

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Analysis of the Interaction of Endogenous Retrovirus ev21 and Avian Leukosis Virus Subgroup J in Late-feathering Chicken

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Analysis of the Interaction of Endogenous Retrovirus ev21 an...