检索结果-南通市图书馆

TRIM33 plays a critical role in regulating dendritic cell differentiation and homeostasis by modulating irf8 and Bcl2l11 transcription

在线全文

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2024年第7期21卷 752-769页

作者： Xiangyi Shen Xiaoguang Li Tao Wu Tingting Guo Jiaoyan Lv Zhimin He Maocai Luo Xinyi Zhu Yujie Tian Wenlong Lai Chen Dong Xiaoyu Hu Li Wu Institute for Immunology School of Basic Medical SciencesTsinghua University100084BeijingChina Joint Graduate Program of Peking-Tsinghua-National Institute of Biological Sciences School of Life SciencesTsinghua University100084BeijingChina Tsinghua-Peking Center for Life Sciences Tsinghua University100084BeijingChina Beijing Key Laboratory for Immunological Research on Chronic Diseases 100084BeijingChina Westlake University School of Medicine Hangzhou310024China

The development of distinct dendritic cell(DC)subsets,namely,plasmacytoid DCs(pDCs)and conventional DC subsets(cDC1s and cDC2s),is controlled by specific transcription ***8 is essential for the fate specification of ***,how the expression of irf8 is regulated is not fully *** this study,we identified TRIM33 as a critical regulator of DC differentiation and ***33 deletion in Trim33fl/fl Cre-ERT2 mice significantly impaired DC differentiation from hematopoietic progenitors at different developmental ***33 deficiency downregulated the expression of multiple genes associated with DC differentiation in these ***33 promoted the transcription of irf8 to facilitate the differentiation of cDC1s by maintaining adequate CDK9 and Ser2 phosphorylated RNA polymerase II(S2 Pol II)levels at irf8 gene ***,TRIM33 prevented the apoptosis of DCs and progenitors by directly suppressing the PU.1-mediated transcription of Bcl2l11,thereby maintaining DC *** together,our findings identified TRIM33 as a novel and crucial regulator of DC differentiation and maintenance through the modulation of irf8 and Bcl2l11 *** finding that TRIM33 functions as a critical regulator of both DC differentiation and survival provides potential benefits for devising DC-based immune interventions and therapies.

关键词： Dendritic cell TRIM33 Transcription regulation irf8 BIM

Transcription factor irf8 controls Thl-like regulatory T-cell function

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2016年第6期13卷 785-794页

作者： Wonyong Lee Hyeong Su Kim Song Yi Baek Gap Ryol Lee Department of Life Science Sogang University 35 Baekbeom-ro Mapo-gu Seou1121-742 Korea

Recent studies have suggested that regulatory T （Treg） cells comprise a heterogeneous population that regulates various aspects of the immune response, and that Treg cells use the factors that are expressed in their target cells to regulate them. We searched for factors that regulate Thl response in Treg cells using a meta-analysis. In the process, we discovered that transcription factor interferon regulatory factor 8 （I RF8） was selectively expressed in Treg and Th I cells. irf8-deficient Treg cells showed defective expression of CXCR3 and aberrant expression of the 114 and 1117genes. Upon treatment with alpha galactosyI-C18-ceramide （aGaI-C18-Cer）, irf8-deficient mice showed defective Treg cell recruitment in the liver. Eliciting Thl immune response by anti-CD40 antibody injection in mice induced irf8 expression in Treg cells. The expression of irf8 was induced by Foxp3 in Treg cells, irf8 had no effect on T-bet expression in Treg and vice versa. Thus, our results strongly suggest that irf8 controls Thl immune response in Treg cells independent of T-bet.

关键词： CXCR3 irf8 regulatory T cell Thl-like

irf4 and irf8 expression are associated with clinical phenotype and clinico-hematological response to hydroxyurea in essential thrombocythemia

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Medicine 2022年第3期16卷 403-415页

作者： Xiao Huang Tingting Ma Yongmei Zhu Bo Jiao Shanhe Yu Kankan Wang Jian-Qing Mi Ruibao Ren Shanghai Institute of Hematology State Key Laboratory for Medical GenomicsCollaborative Innovation Center of HematologyNational Research Center for translational Medicine(Shanghai)Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200025China

The morbidity and mortality of myeloproliferative neoplasms(MPNs)are primarily caused by arterial and venous complications,progression to myelofibrosis,and transformation to acute ***,identifying molecular-based biomarkers for risk stratification of patients with MPNs remains a *** have previously shown that interferon regulatory factor-8(irf8)and irf4 serve as tumor suppressors in myeloid *** this study,we evaluated the expression of irf4 and irf8 and the JAK2V617F mutant allele burden in patients with *** with decreased irf4 expression were correlated with a more developed MPN phenotype in myelofibrosis(MF)and secondary AML(sAML)transformed from MPNs versus essential thrombocythemia(ET).Negative correlations between the JAK2V617F allele burden and the expression of irf8(Pirf4(Pirf4 expression(P8 expression was negatively correlated with the JAK2V617F allele burden(Pirf4 and irf8 expression compared with patients in the PR and NR *** the 12-month therapy period,low irf4 and irf8 expression were independently associated with the unfavorable response to HU and high WBC *** data indicate that the expression of irf4 and irf8 was associated with the MPN phenotype,which may serve as biomarkers for the response to HU in ET.

关键词： myeloproliferative neoplasms irf4 irf8 hydroxyurea essential thrombocythemia

Nebulized Mycobacterium vaccae protects against asthma by attenuating the imbalance of irf4/irf8 expression in dendritic cells

在线全文

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Biomedicine 2022年第12期12卷 520-529页

作者： Qi-Xiang Sun Si-Yue Xu Lao-Dong Li Huan Xiao Qian-Nan Zhang Chao-Qian Li Department of Respiratory Medicine The First Affiliated Hospital of Guangxi Medical UniversityNanningChina

Objective:To assess the effects of nebulized inhaled Mycobacterium vaccae on allergic airway inflammation,airway hyperresponsiveness,and Th1/Th2 cell imbalance in mice with ovalbumin(OVA)-induced ***:Mice received OVA sensitization and challenge for establishment of the asthmatic *** intervention,mice received Mycobacterium vaccae nebulization once every other day from the first day of sensitization to the day before *** challenge,pulmonary histological analysis and airway responsiveness measurement were *** addition,Th1/Th2 cytokines and OVA-specific IgE levels in bronchoalveolar lavage fluid were measured by ***1/Th2 subset ratios and the expression of interferon-regulatory factor 4(irf4),irf8 and Toll-like receptor 4(TLR4)in dendritic cells were evaluated by flow ***:Severe inflammatory infiltration and airway hyperresponsiveness were observed in OVA-induced asthmatic *** mice showed higher Th2 cytokine concentration and increased percentage of Th2 cells,along with lower Th1 cytokine concentration and reduced percentage of Th1 cells compared with the normal ***,an imbalance of irf4^(+)and irf8^(+)in dendritic cells was found in asthmatic *** inhaled Mycobacterium vaccae reduced airway hyperresponsiveness and inflammation in OVA-induced asthmatic *** addition,nebulized inhaled Mycobacterium vaccae enhanced TLR4 and irf8 expression,and alleviated the imbalance of Th1/Th2 as well as irf4^(+)and irf8^(+)in dendritic ***:Nebulized inhaled Mycobacterium vaccae protects against asthma by alleviating the imbalance of Th1/Th2 and irf4/irf8 in OVA-induced asthmatic mice.

关键词： Mycobacterium vaccae Asthma Nebulization irf4 irf8 TLR4

irf4 and irf8： governing the virtues of B lymphocytes

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers in Biology 2014年第4期9卷 269-282页

作者： Vipul SHUKLA Runqing LU Department of Genetics Cell Biology & Anatomy University of Nebraska Medical Center Omaha NE 68198 USA

Interferon regulatory factor 4 （irf4） and irf8 are critical regulators of immune system development and function. In B lymphocytes, irf4 and irf8 have been shown to control important events during their development and maturation including pre-B cell differentiation, induction of B cell tolerance pathways, marginal zone B cell development, germinal center reaction and plasma cell differentiation. Mechanistically, irf4 and irf8 are found to function redundantly to control certain stages of B cell development, but in other stages, they function nonredundantly to play distinct roles in B cell biology. In line with their essential roles in B cell development, deregulated expressions of irf4 and irf8 have been associated to the pathogenesis of several B cell malignancies and diseases. Recent studies have elucidated diverse transcriptional networks regulated by irf4 and irf8 at distinct B cell developmental stages and related malignancies. In this review we will discuss the recent advances for the roles of irf4 and irf8 during B cell development and associated diseases.

关键词： irf4 irf8 B lymphocytes transcriptional regulation leukemia and lymphoma B cell development

irf8基因降表达对大鼠肺泡巨噬细胞缺氧复氧损伤的影响

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

武汉大学学报（医学版） 2021年第5期42卷 737-742页

作者：冯浩洁范涛张霖卢子龙沈小康耿庆武汉大学人民医院胸外科湖北武汉430060

目的:观察干扰素调节因子8(irf8)基因降表达对肺泡巨噬细胞NR8383缺氧复氧(HR)损伤的影响,探讨irf8基因在该细胞缺氧复氧过程中的作用。方法:取对数生长期的大鼠肺泡巨噬细胞NR8383随机分为正常对照组、阴性对照组、干扰空质粒组、irf8... 详细信息

目的:观察干扰素调节因子8(irf8)基因降表达对肺泡巨噬细胞NR8383缺氧复氧(HR)损伤的影响,探讨irf8基因在该细胞缺氧复氧过程中的作用。方法:取对数生长期的大鼠肺泡巨噬细胞NR8383随机分为正常对照组、阴性对照组、干扰空质粒组、irf8低表达组。正常对照组常氧条件下培养,其余各组行缺氧6 h,复氧24 h处理。CCK8、LDH法检测细胞增殖率,酶联免疫吸附试验(ELISA)检测炎性因子的表达。结果:irf8干扰质粒转染成功,可明显降低HR处理前irf8蛋白的表达。irf8低表达组HR处理后细胞增殖率及细胞活性都显著降低(Pirf8低表达组肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β、IL-6表达显著降低,IL-10升高,差异有统计学意义(Pirf8基因降表达在体外可参与大鼠肺泡巨噬细胞NR8383缺氧复氧损伤过程,抑制肺泡巨噬细胞细胞增殖率和细胞活性,并且抑制该细胞促炎细胞因子的表达,促进抗炎细胞因子的表达。

关键词： irf8 肺缺血再灌注损伤肺泡巨噬细胞缺氧复氧

Establishment of The Zebrafish Acute Myeloid Leukemia(AML)Model on irf8-/-

在线全文

学校读者我要写书评

暂无评论

Establishment of The Zebrafish Acute Myeloid Leukemia(AML)Mo...

脉冲射频大鼠CCI模型DRG对脊髓小胶质细胞irf8表达的影响

第二届全国发育生物学大会

作者： Yanyan Shi Fangying Zhao Yujia Peng Yuelan Hou Yandong Zhan Honghui Huang Hua Ruan Lingfei Luo Li Li The State Key Laboratory Breeding Base of Bioresources and Eco-environments Key Laboratory of Freshwater Fish Reproduction and DevelopmentMinistry of EducationLaboratory of Molecular Developmental BiologySchool of Life SciencesSouthwest University

irf8,also named ICSBP,is a critical transcriptional factor of interferon regulatory factor(TRF)family in hematopoietic cells development,particular normal *** irf8 defect mouse,the severe myeloid disorder,CML appears with ***,more than half of human CML and AML patients show compromised expression of ***,this kind of leukemia presents increased drug-resistance in ***,looking for the mechanism under irf8 function and efficient drugs are the ***,we created several irf8zebrafish mutant alleles taking advantage of the *** germline transmission homozygous mutants were identified which could survive beyond *** characterization data indicated that irfS was a macrophage-fate determine factor in primitive ***,loss of irf8 in the mutants led to extensive expanding of neutrophils and eventually the lethal AML manifesting significant blast crisis progression in adult *** several signal pathways related to tumorigenesis distorted *** study indicated that irf8 mainly functioned to regulate myeloid cells apoptosis,and it served as a leukemia suppressor gene beyond *** of the clinical common anti-leukemia drug-Topotecan,but not Imatinib,could obviously alleviate the irf8 mutant *** together,we established a zebrafish AML model which would be quite helpful in the future genetic and chemical study of AML.

关键词： irf8 myelopoiesis myeloid leukemia zebrafish

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

中国疼痛医学杂志 2016年第11期22卷 816-822页

作者：方向宇徐雪汝刘荣国米立梅许云禄福建医科大学省立临床学院福建省立医院疼痛科福州350001 福建医科大学药理学院福州350000

目的:研究脉冲射频(pulsed radiofrequency,PRF)大鼠慢性坐骨神经缩窄损伤(chronic constriction injury of sciatic nerve,CCI)模型背根神经节(dorsal root ganglion,DRG)能否抑制脊髓小胶质细胞中干扰素调节因子8(interferon regulatory factor 8,irf8)表达而减轻疼痛。方法:雄性SD大鼠120只随机均分为4组:假手术组(Sham)、CCI组、CCI+PRF组、CCI+NPRF组,每组30只。采用结扎一侧坐骨神经主干制作大鼠CCI模型。CCI+PRF组于制模后第7 d,对术侧L4-5背根神经节进行脉宽20 ms、脉冲频率2 Hz,42℃,持续6 min的PRF治疗。CCI+NPRF组仅在相同位置放置射频电极,无脉冲治疗,持续6 min。制模前测量各组大鼠基础阈值,制模后1、3、5、7 d,PRF后1、3、5、7、14 d四组大鼠均接受行为学、痛阈测定。制模后7 d,PRF后7、14 d western blot检测irf8蛋白表达。制模后7 d,PRF后14 d免疫荧光法检测Iba1表达。结果:与假手术组比,CCI组、CCI+PRF组、CCI+NPRF组大鼠于神经损伤后痛阈均显著降低(Pirf8、Iba1表达上调(Pirf8、Iba1表达则相应减少。结论:脉冲射频可缓解大鼠CCI模型神经病理性疼痛,其机制可能与抑制大鼠脊髓irf8表达、小胶质细胞活化有关。

关键词：神经病理性疼痛脉冲射频背根神经节小胶质细胞 irf8

鞘内注射irf8反义寡核苷酸对CCI模型大鼠痛阈的影响

在线全文

学校读者我要写书评

暂无评论

中国疼痛医学杂志 2015年第5期21卷 351-356页

作者：刘荣国徐雪汝米立梅许云禄陈彦青福建医科大学省立临床医学院福建省立医院疼痛科福州350001 福建医科大学药理学院福州350000

目的:探讨鞘内注射小胶质细胞干扰素调节因子8(interferon regulatory factor 8,irf8)反义寡核苷酸(oligodeoxynucleotide,ODN)对大鼠慢性坐骨神经缩窄损伤(chronic constriction injury of sciatic nerve,CCI)模型疼痛行为的影响。方法:雄性SD大鼠40只随机分为4组:Sham组、AS组、MM组、NS组,每组10只。除Sham组外,其他三组均鞘内置管并制备CCI模型,AS组、MM组、NS组依次于CCI后12 h鞘内注射irf8反义/错义ODN、等体积生理盐水,1次/d,连续6 d。制模前测量各组大鼠的基础阈值,制模后1、3、5、7、10、12、14 d对大鼠进行疼痛行为测定。制模后7、14 d western blot检测irf8、离子钙接合蛋白Iba1表达。结果:与Sham组比较,NS组、MM组大鼠神经损伤后右后爪机械痛阈和热痛阈均显著降低(Pirf8、Iba1蛋白表达显著增强(Pirf8、Iba1蛋白表达,AS组较MM组、NS组显著减弱(Pirf8、Iba1蛋白表达较第7 d增强(Pirf8反义寡核苷酸可抑制脊髓内irf8表达和小胶质细胞活化而缓解CCI大鼠神经病理性疼痛。

关键词：神经病理性疼痛 irf8 反义寡核苷酸小胶质细胞