检索结果-南通市图书馆

维普期刊数据库博看期刊

实时超声弹性成像参数、血清CA153、igf1及HE4水平与早期宫颈癌临床病理特征的关系及其预测价值分析

在线全文

学校读者我要写书评

暂无评论

现代生物医学进展 2024年第7期24卷 1335-1339页

作者：胡伯媛许厚本杨耀徐星肖芳言娜湖南中医药高等专科学校附属第一医院(湖南省直中医医院)超声医学科湖南株洲412000 湖南中医药高等专科学校附属第一医院(湖南省直中医医院)妇科湖南株洲412000

目的:探讨实时超声弹性成像参数、血清糖类抗原153(CA153)、胰岛素生长因子1(igf1)及人附睾分泌蛋白4(HE4)水平与早期宫颈癌临床病理特征的关系及其预测价值。方法:选取2022年1月~2023年6月本院收治的85例早期宫颈癌患者为宫颈癌组,另... 详细信息

目的:探讨实时超声弹性成像参数、血清糖类抗原153(CA153)、胰岛素生长因子1(igf1)及人附睾分泌蛋白4(HE4)水平与早期宫颈癌临床病理特征的关系及其预测价值。方法:选取2022年1月~2023年6月本院收治的85例早期宫颈癌患者为宫颈癌组,另取同期收治的85例宫颈癌前病变患者为宫颈癌前病变组。两组均进行实时超声弹性成像检查,并测定血清CA153、igf1及HE4水平。探讨实时超声成像、血清CA153、igf1及HE4与宫颈癌患者临床病理特征的关系。采用受试者工作特征曲线(ROC)分析实时超声弹性成像参数、血清CA153、igf1及HE4对早期宫颈癌患者的诊断价值。结果:相较于宫颈癌前病变组,宫颈癌组弹性应变比值和弹性图像压放指数均明显升高(P153、igf1及HE4水平均明显升高(P153、igf1及HE4水平均明显高于FIGO分期Ⅰ期、中高分化程度患者(P153、igf1、HE4诊断早期宫颈癌的曲线下面积(AUC)分别为0.811、0.807、0.760、0.649、0.764,联合诊断的AUC最大,为0.944。结论:实时超声弹性成像参数、血清CA153、igf1及HE4与早期宫颈癌的分期和分化程度有关,且对诊断早期宫颈癌均具有一定的临床价值,以联合诊断的价值更高。

关键词：实时超声弹性成像 CA153 igf1 HE4 早期宫颈癌诊断价值

Serine metabolism orchestrates macrophage polarization by regulating the igf1–p38 axis

在线全文

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2022年第11期19卷 1263-1278页

作者： Xiao Shan Penghui Hu Lina Ni Long Shen Yanan Zhang Zemin Ji Yan Cui Meihua Guo Haoan Wang Liyuan Ran Kun Yang Ting Wang Lei Wang Bin Chen Zhi Yao Vingjie Wu Qiujing Yu Tianjin Institute of Immunology Key Laboratory of Immune Microenvironment and Disease of the Ministry of EducationThe Province and Ministry Co-sponsored Collaborative Innovation Center for Medical EpigeneticsTianjin Key Laboratory of Inflammation BiologyDepartment of ImmunologySchool of Basic Medical SciencesTianjin Medical University Division of Infectious Disease Second Hospital of Tianjin Medical UniversityTianjin300070China Institute for Genome Engineered Animal Models of Human Diseases National Center of Genetically Engineered Animal Models for International ResearchDalian Medical UniversityDalian116044LiaoningChina Shandong Provincial Hospital School of Laboratory Animal and Shandong Laboratory Animal CenterScience and Technology Innovation CenterShandong First Medical University and Shandong Academy of Medical SciencesJinan250021ShandongChina The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics Department of Pharmacology and Tianjin Key Laboratory of Inflammation BiologySchool of Basic Medical SciencesTianjin Medical UniversityTianjin300070China Tianjin Institute of Immunology Key Laboratory of Immune Microenvironment and Disease of the Ministry of EducationDepartment of ImmunologySchool of Basic Medical SciencesTianjin Medical UniversityTianjinChina Department of Molecular Pathobiology New York University College of DentistryNew YorkNY10010USA

Serine metabolism is reportedly involved in immune cell functions, but whether and how serine metabolism regulates macrophage polarization remain largely unknown. Here, we show that suppressing serine metabolism, either by inhibiting the activity of the key enzyme phosphoglycerate dehydrogenase in the serine biosynthesis pathway or by exogenous serine and glycine restriction, robustly enhances the polarization of interferon-γ-activated macrophages (M(IFN-γ)) but suppresses that of interleukin-4-activated macrophages (M(IL-4)) both in vitro and in vivo. Mechanistically, serine metabolism deficiency increases the expression of igf1 by reducing the promoter abundance of S-adenosyl methionine-dependent histone H3 lysine 27 trimethylation. igf1 then activates the p38-dependent JAK–STAT1 axis to promote M(IFN-γ) polarization and suppress STAT6-mediated M(IL-4) activation. This study reveals a new mechanism by which serine metabolism orchestrates macrophage polarization and suggests the manipulation of serine metabolism as a therapeutic strategy for macrophage-mediated immune diseases.

关键词： Serine metabolism PHGDH Macrophage polarization SAM igf1 p38

Characterization and expression analysis of gonad specific igf3 in the medaka ovary

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Aquaculture and Fisheries 2022年第3期7卷 259-268页

作者： Jiale Xie Ying Zhong Yuli Zhao Wenjie Xie Jing Guo Lang Gui Mingyou Li International Research Center for Marine Biosciences Ministry of Science and TechnologyShanghai Ocean UniversityChina Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai201306China

The gonad specific expression of insulin-like growth factor 3(igf3)has suggested an important role of igf3 in fish reproduction.In this study,medaka igf3 was isolated and its expression patterns were compared with igf1,and germ cell gene vasa in adult medaka ovary.Molecular cloning and sequencing showed that the open reading frame(ORF)of medaka igf3 was comprised of 501 nucleotides and encoded 166 amino acid residues.Reverse transcription polymerase chain reaction(RT-PCR)analysis showed that igf3 RNA was specifically expressed in developing embryos and adult gonads of both sexes.Real time quantitative PCR(q-PCR)indicated that igf3 expression was gradually increased during oogenesis,and reached its highest level at stage V.Using Chromogenic and fluorescent in situ hybridization,igf3 was shown to be present in the germ cells of oogonia and in oocytes at stages I-III,as well as abundant in granulosa cells and theca cells of oocytes at stages IV-V.In addition,igf3 and igf1 were expressed mutually in the outer theca cells of stage V oocytes in ovary.Collectively,we demonstrated that gonad specific igf3 could mark medaka ovarian somatic cells and germ cells.These findings highlight the importance of igf3 during ovarian development.

关键词： igf3 igf1 Medaka Ovary

igf1-PI3K-induced physiological cardiac hypertrophy:Implications for new heart failure therapies,biomarkers,and predicting cardiotoxicity

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Sport and Health Science 2021年第6期10卷 637-647,I0001页

作者： Sebastian Bass-Stringer Celeste M.K.Tai Julie R.McMullen Baker Heart and Diabetes Institute MelbourneVIC 3004Australia Department of Physiology Anatomy and MicrobiologyLa Trobe UniversityBundooraVIC 3086Australia Department of Diabetes Central Clinical SchoolMonash UniversityMelbourneVIC 3004Australia Department of Physiology and Department of Medicine Alfred Hospital Monash UniversityMelbourneVIC 3004Australia

Heart failure represents the end point of a variety of cardiovascular diseases.It is a growing health burden and a leading cause of death worldwide.To date,limited treatment options exist for the treatment of heart failure,but exercise has been well-established as one of the few safe and effective interventions,leading to improved outcomes in patients.However,a lack of patient adherence remains a significant barrier in the implementation of exercise-based therapy for the treatment of heart failure.The insulin-like growth factor 1(igf1)phosphoinositide 3-kinase(PI3K)pathway has been recognized as perhaps the most critical pathway for mediating exercisedinduced heart growth and protection.Here,we discuss how modulating activity of the igf1PI3K pathway may be a valuable approach for the development of therapies that mimic the protective effects of exercise on the heart.We outline some of the promising approaches being investigated that utilize PI3K-based therapy for the treatment of heart failure.We discuss the implications for cardiac pathology and cardiotoxicity that arise in a setting of reduced PI3K activity.Finally,we discuss the use of animal models of cardiac health and disease,and genetic mice with increased or decreased cardiac PI3K activity for the discovery of novel drug targets and biomarkers of cardiovascular disease.

关键词： Cardiac protection Cardiotoxicity Exercise Heart failure igf1 PI3K Therapies

Sequential activation of uterine epithelial igf1R by stromal igf1 and embryonic igf2 directs normal uterine preparation for embryo implantation

在线全文

学校读者我要写书评

暂无评论

Journal of Molecular Cell Biology 2021年第9期13卷 646-661页

作者： Chan Zhou Meiying Lv Peike Wang Chuanhui Guo Zhangli Ni Haili Bao Yedong Tang Han Cai Jinhua Lu Wenbo Deng Xiaoyu Yang Guoliang Xia Haibin Wang Chao Wang Shuangbo Kong State Key Laboratory of Agrobiotechnology College of Biological SciencesChina Agricultural UniversityBeijingChina'2 Fujian Provincial Key Laboratory of Reproductive Health ResearchSchool of MedicineXiamen UniversityXiamenChina'3 Fuzhou Hospital of Traditional Chinese Medicine Affiliated to Fujian University of Traditional Chinese MedicineFuzhouChina'4 Department of Obstetrics and GynecologyThe First Affiliated Hospital of Xiamen UniversityXiamenChina'5 Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western ChinaCollege of Life ScienceNingXia UniversityYinchuanChina

Embryo implantation in both humans and rodents is initiated by the attachment of a blastocyst to the uterine epithelium.For blastocyst attachment,the uterine epithelium needs to transform at both the structural and molecular levels first,and then initiate the interaction with trophectoderm.Any perturbation during this process will result in implantation failure or long-term adverse pregnancy outcomes.Endocrine steroid hormones,which function through nuclear receptors,combine with the local molecules produced by the uteri or embryo to facilitate implantation.The insulin-like growth factor(igf)signaling has been reported to play a vital role during pregnancy.However,its physiological function during implantation remains elusive.This study revealed that mice with conditional deletion of igflr gene in uteri suffered from subfertility,mainly due to the disturbed uterine receptivity and abnormal embryo implantation.Mechanistically,we uncovered that in response to the nidatory estrogen on D4 of pregnancy,the epithelial igf1R,stimulated by the stromal cell-produced igf1,facilitated epithelial STAT3 activation to modulate the epithelial depolarity.Furthermore,embryonic derived igf2 could activate both the epithelial ERK1/2 and STAT3 signaling through igf1R,which was critical for the transcription of Cox2 and normal attachment reaction.In brief,our data revealed that epithelial igf1R was sequentially activated by the uterine stromal igf1 and embryonic igf2 to guarantee normal epithelium differentiation during the implantation process.

关键词： igf1R igf1 igf2 uterine epithelium implantation blastocyst

巴克夏猪、霍寿黑猪及其杂交F1代igf1基因多态性分析

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国畜牧杂志 2023年第10期59卷 144-147页

作者：周超群杨昊王昊宇杨兵许金根安徽科技学院动物科学学院动物营养调控与健康安徽省重点实验室安徽凤阳233100

本实验采用PCR-RFLP方法分析巴克夏猪、霍寿黑猪及其杂交F1代igf1基因Hha I酶切位点多态性,并用生物学软件比对猪igf1基因编码区序列以及预测SNP对mRNA二级结构的影响。结果表明:巴克夏猪igf1基因只检测出2种基因型,优势基因型为BB,优... 详细信息

本实验采用PCR-RFLP方法分析巴克夏猪、霍寿黑猪及其杂交F1代igf1基因Hha I酶切位点多态性,并用生物学软件比对猪igf1基因编码区序列以及预测SNP对mRNA二级结构的影响。结果表明:巴克夏猪igf1基因只检测出2种基因型,优势基因型为BB,优势等位基因为B,处于低度多态;霍寿黑猪igf1基因存在3种基因型,基因型BB的频率最高,优势等位基因为B,处于中度多态;巴克夏猪×霍寿黑猪igf1基因存在3种基因型,基因型AB的频率最高,优势等位基因为B,处于中度多态。序列分析表明,该单核苷酸变异位于猪igf1基因编码区c.258A>G(GenBank登录号:DQ784687),未引起编码的第86位丙氨酸发生变化,但改变了mRNA的最小自由能。本研究结果为猪igf1基因用作生长性能的分子标记提供了基础。

关键词：猪 igf1 基因多态性序列分析

巴克夏猪、霍寿黑猪及其杂交F代igf1基因多态性分析

在线全文

学校读者我要写书评

暂无评论

中国畜牧杂志 2023年

作者：周超群杨昊王昊宇杨兵许金根安徽科技学院动物科学学院动物营养调控与健康安徽省重点实验室

本实验采用PCR-RFLP方法分析巴克夏猪、霍寿黑猪及其杂交F1代igf1基因Hha I酶切位点多态性，并用生物学软件比对猪igf1基因编码区序列以及预测SNP对mRNA二级结构的影响。结果表明：巴克夏猪igf1基因只检测出2种基因型，优势基因型为BB... 详细信息

本实验采用PCR-RFLP方法分析巴克夏猪、霍寿黑猪及其杂交F1代igf1基因Hha I酶切位点多态性，并用生物学软件比对猪igf1基因编码区序列以及预测SNP对mRNA二级结构的影响。结果表明：巴克夏猪igf1基因只检测出2种基因型，优势基因型为BB，优势等位基因为B，处于低度多态；霍寿黑猪igf1基因存在3种基因型，基因型BB的频率最高，优势等位基因为B，处于中度多态；巴克夏猪×霍寿黑猪igf1基因存在3种基因型，基因型AB的频率最高，优势等位基因为B，处于中度多态。序列分析表明，该单核苷酸变异位于猪igf1基因编码区c.258A>G(GenBank登录号：DQ784687)，未引起编码的第86位丙氨酸发生变化，但改变了mRNA的最小自由能。本研究结果为猪igf1基因用作生长性能的分子标记提供了基础。

关键词：猪 igf1 基因多态性序列分析

miR-376b-5p调控绵羊产羔性状相关基因igf1的表达

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

中国农业大学学报 2023年第4期28卷 108-117页

作者：李文韬周丽娟刘子嶷王鹏储明星刘玉芳河北工程大学生命科学与食品工程学院河北邯郸056021 中国农业科学院北京畜牧兽医研究所农业农村部动物遗传育种与繁殖重点实验室北京100193 河北省沧州市东光县农业农村局河北沧州061600

为探讨miR-376b-5p靶向调控igf1影响绵羊多羔性状的分子机制,本研究根据产羔记录选取高、低产羔数小尾寒羊各5只,同期发情处理后,采集卵泡期卵巢及其他6个组织,通过RT-qPCR、Western blot和双荧光素酶报告基因检测系统分析miR-376b-5p与... 详细信息

为探讨miR-376b-5p靶向调控igf1影响绵羊多羔性状的分子机制,本研究根据产羔记录选取高、低产羔数小尾寒羊各5只,同期发情处理后,采集卵泡期卵巢及其他6个组织,通过RT-qPCR、Western blot和双荧光素酶报告基因检测系统分析miR-376b-5p与igf1在高、低产羔数小尾寒羊各组织中的表达量;并确定二者之间的靶向关系;随后,将体外合成的miR-376b-5p模拟物(mimic)和抑制物(inhibitor)转染至绵羊卵巢颗粒细胞中,检测miR-376b-5p对igf1表达水平的调控情况,同时对多羔性状TGF-β信号通路中TGFβ1和PTGS2标志因子的表达进行分析。结果表明:1)组织表达谱显示,igf1在绵羊卵巢组织中的表达量显著高于其他组织(Pigf1在高产羔数小尾寒羊卵巢组织中的表达量极显著高于低产羔数小尾寒羊(P1);miR-376b-5p定量结果显示其表达趋势与igf1相反(P1);2)在线软件预测和双荧光素酶报告系统检测显示,miR-376b-5p可靶向结合igf1 3′UTR序列;3)在绵羊卵巢颗粒细胞中过表达miR-376b-5p后,igf1蛋白和mRNA表达水平均显著下降(Pigf1的表达;4)在绵羊卵巢颗粒细胞中过表达miR-376b-5p后,TGF-β信号通路中TGFβ1、PTGS2的表达量均显著低于阴性对照组(Pigf1 3′UTR区域,并通过抑制igf1和TGF-β信号通路调控颗粒细胞增殖及绵羊繁殖,为进一步揭示绵羊多羔性状形成的分子机制及分子育种提供理论基础。

关键词： miR-376b-5p igf1 绵羊卵巢

超排处理对家兔早期胚胎发育及igf1、EGF表达的影响

在线全文

学校读者我要写书评

暂无评论

中国畜牧杂志 2023年第1期59卷 203-207页

作者：伏晓玉柳苗苗铁雅楠王志亮杨喜喜王欣荣甘肃农业大学动物科学技术学院甘肃兰州730070

为探究超数排卵对家兔早期胚胎发育及igf1、EGF基因表达的影响,本实验选取成年健康家兔48只,随机分为超排组和对照组,对超排组家兔注射70 IU PMSG+100 IU hCG进行超排处理,观察超排处理对排卵、胚胎体外发育的影响,并通过qRT-PCR分析igf... 详细信息

为探究超数排卵对家兔早期胚胎发育及igf1、EGF基因表达的影响,本实验选取成年健康家兔48只,随机分为超排组和对照组,对超排组家兔注射70 IU PMSG+100 IU hCG进行超排处理,观察超排处理对排卵、胚胎体外发育的影响,并通过qRT-PCR分析igf1和EGFmRNA在早期胚胎中的表达差异。结果显示:超排组家兔的平均充血卵泡数、平均排卵点数、平均胚胎收集数和畸形率均显著高于对照组,4-细胞期和8-细胞期的胚胎发育率显著低于对照组。无论是对照组还是超排组,igf1基因均在4-细胞期表达量最高,而EGF基因在2-细胞期表达量最高。超排组中,igf1基因在2-细胞期胚胎中的表达量显著低于对照组,在4-细胞期和8-细胞期胚胎中与对照组相比差异不显著,而EGF基因在2-细胞期胚胎中的表达量显著高于对照组,在4-细胞期和8-细胞期胚胎中则显著低于对照组。综上可知,外源促性腺激素可能通过改变igf1和EGF在早期胚胎中的表达量而影响家兔早期胚胎的发育。

关键词：外源激素家兔胚胎发育 igf1 EGF