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hupo powder promotes autophagy of menstrual blood-derived stem cells from patients with endometriosis

Journal of Traditional Chinese Medical Sciences 2023年第2期10卷 179-185页

作者： Yuejian Zhang Changxiang Li Conglu Sui Xiuping Zhang Ya'nan Guo Tiantian He Taoxiu Lin Xiaona Ma The Third School of Clinical Medicine Beijing University of Chinese MedicineBeijing100029China School of Traditional Chinese Medicine Beijing University of Chinese MedicineBeijing102488China Department of Gynecology Beijing University of Chinese Medicine Third Affiliated HospitalBeijing100029China

Objective:To explore the effect of hupo powder(HP)on autophagy in menstrual blood-derived stem cells(MenSCs)with endometriosis(EMT).Methods:EMT MenSCs(E-MenSCs)and healthy MenSCs(H-MenSCs)were isolated from the menstrual blood of patients with EMT and healthy female participants,*** identified their stem cells’characteristics via adipogenic and osteogenic *** male SpragueeDawley rats received 0.9% NaCl and HP-dispensing granules by gastric irrigation to prepare blank serum and medicated serum,*** used serum concentrations of 5%,10%,and 20%,each at administered times of 12,24,and 48 h to select the best *** cells were divided into three groups:blank serum of the control group,blank serum of the model group,and medicated serum of the HP group.H-MenSCs were used in the control group,while E-MenSCs were used in the model and HP *** analyzed cell viability using a cell counting kit-8 assay,observed cell morphology,evaluated the amounts of auto-phagosomes and autolysosomes by transmission electron microscopy,and detected the protein expression of autophagy markers(LC3-II and Beclin1)by Western ***:E-MenSCs and H-MenSCs became long fusiform with a diffuse radial pattern,forming lipid droplets and calcium nodules after adipogenic and osteogenic *** then used the best conditiond 20% serum and 48 hdfor the subsequent *** contrast to the model group,the HP group exhibited lower cell viability(=0.007),larger amounts of autophagosomes and autolysosomes(Phupo powder can promote autophagy in E-MenSCs,which might be one of the mechanisms underlying its therapeutic effects.

关键词： hupo powder Endometriosis Menstrual blood-derived stem cells Autophagy Beclin1 LC3-II

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