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Development of methods for detecting the fate of mesenchymal stem cells regulated by bone bioactive materials

Bioactive Materials 2021年第3期6卷 613-626页

作者： Jiang Le Liu Zhongqun Wang Zhaoyan Su Yijun Wang Yingjin Wei Yaojie Jiang Yanan Jia Zhanrong Ma Chunyang Gang Fangli Xu Nan Zhao Lingyun Wang Xiumei Wu Qiong Lu Xiong Sun Xiaodan State Key Laboratory of New Ceramics and Fine Processing School of Materials Science and EngineeringTsinghua UniversityBeijing100084People's Republic of China Key Laboratory of Advanced Materials of Ministry of Education of China School of Materials Science and EngineeringTsinghua UniversityBeijing100084People's Republic of China Key Lab of Advanced Technologies of Materials of Ministry of Education School of Materials Science and EngineeringSouthwest Jiaotong UniversityChengdu610031People's Republic of China MOE Key Laboratory of Bioinformatics Tsinghua UniversityBeijing100084People's Republic of China Center for Synthetic and Systems Biology Tsinghua UniversityBeijing100084People's Republic of China School of Life Sciences Tsinghua UniversityBeijing100084People's Republic of China

The fate of mesenchymal stem cells(MSCs)is regulated by biological,physical and chemical *** in biotechnology and materials science promoted the occurrence of bioactive materials which can provide physical and chemical signals for MSCs to regulate their *** order to design and synthesize materials that can precisely regulate the fate of MSCs,the relationship between the properties of materials and the fate of mesenchymal stem cells need to be clarified,in which the detection of the fate of mesenchymal stem cells plays an important *** the past 30 years,a series of detection technologies have been developed to detect the fate of MSCs regulated by bioactive materials,among which high-throughput technology has shown great advantages due to its ability to detect large amounts of data at one *** this review,the latest research progresses of detecting the fate of MSCs regulated by bone bioactive materials(BBMs)are systematically reviewed from traditional technology to high-throughput technology which is emphasized ***,current problems and the future development direction of detection technologies of the MSCs fate regulated by BBMs are *** aim of this review is to provide a detection technical framework for researchers to establish the relationship between the properties of BMMs and the fate of MSCs,so as to help researchers to design and synthesize BBMs better which can precisely regulate the fate of MSCs.

关键词： Bone bioactive materials high-throughput technology Transcriptomics Single cell RNA-seq Proteomics

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