Development of methods for detecting the fate of mesenchymal stem cells regulated by bone bioactive materials

作     者：Jiang Le Liu Zhongqun Wang Zhaoyan Su Yijun Wang Yingjin Wei Yaojie Jiang Yanan Jia Zhanrong Ma Chunyang Gang Fangli Xu Nan Zhao Lingyun Wang Xiumei Wu Qiong Lu Xiong Sun Xiaodan 

作者机构：State Key Laboratory of New Ceramics and Fine ProcessingSchool of Materials Science and EngineeringTsinghua UniversityBeijing100084People's Republic of China Key Laboratory of Advanced Materials of Ministry of Education of ChinaSchool of Materials Science and EngineeringTsinghua UniversityBeijing100084People's Republic of China Key Lab of Advanced Technologies of Materials of Ministry of EducationSchool of Materials Science and EngineeringSouthwest Jiaotong UniversityChengdu610031People's Republic of China MOE Key Laboratory of BioinformaticsTsinghua UniversityBeijing100084People's Republic of China Center for Synthetic and Systems BiologyTsinghua UniversityBeijing100084People's Republic of China School of Life SciencesTsinghua UniversityBeijing100084People's Republic of China 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2021年第6卷第3期

页      面：613-626页

核心收录：

学科分类：08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

基　　金：supported by the National Key Research and Development Program of China(2016YFB0700802) Natural Sciences Foundation of China(31670991) Major projects of the National Social Science Funding(17ZDA019) the National Natural Science Foundation of China(81671829) Intergovernmental cooperation in science and technology(2016YFE0125300) Tsinghua University Initiative Scientific Research Program(2017THZWYX07) 

主　　题：Bone bioactive materials High-throughput technology Transcriptomics Single cell RNA-seq Proteomics 

摘      要：The fate of mesenchymal stem cells(MSCs)is regulated by biological,physical and chemical *** in biotechnology and materials science promoted the occurrence of bioactive materials which can provide physical and chemical signals for MSCs to regulate their *** order to design and synthesize materials that can precisely regulate the fate of MSCs,the relationship between the properties of materials and the fate of mesenchymal stem cells need to be clarified,in which the detection of the fate of mesenchymal stem cells plays an important *** the past 30 years,a series of detection technologies have been developed to detect the fate of MSCs regulated by bioactive materials,among which high-throughput technology has shown great advantages due to its ability to detect large amounts of data at one *** this review,the latest research progresses of detecting the fate of MSCs regulated by bone bioactive materials(BBMs)are systematically reviewed from traditional technology to high-throughput technology which is emphasized ***,current problems and the future development direction of detection technologies of the MSCs fate regulated by BBMs are *** aim of this review is to provide a detection technical framework for researchers to establish the relationship between the properties of BMMs and the fate of MSCs,so as to help researchers to design and synthesize BBMs better which can precisely regulate the fate of MSCs.