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Algerian mint species:high performance thin layer chromatography quantitative determination of rosmarinic acid and in vitro inhibitory effects on linoleic acid peroxidation

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Journal of Coastal Life Medicine 2014年第12期 986-992页

作者： Brahmi Fatiha Madani Khodir Stévigny Caroline Chibane Mohamed Duez Pierre 13BS Laboratory Faculty of Natural Sciences and LifeUniversity of BejaiaBejaia 06000Algeria Laboratory of Pharmacognosy Bromatology and Human NutritionFaculty of PharmacyFree University of BelgiumCP 205-9B-1050 BrusselsBelgium Faculty of Natural Sciences and Life and Earth Sciences Akli Mohand Oulhadj UniversityBouira 10000Algeria Laboratory of Molecular Biology Institute of Health Sciences and TechnologyUniversity of Mons(UMONS)7000 MonsBelgium

Objective:To determine the quantitative paremeters of rosmarinic acid in Algerian mints,Mentha spicata L.(***),Mentha pulegium *** Mentha rotundifolia(L.)Huds by high performance thin layer chromatography(HPTLC)-densitometric method and screen the effects of these plant extracts on linoleic acid ***:The analyses were performed on HPTLC silica gel 60 F254 plates with chloroform:acetone:formic acid(75:16.5:8.5,v/v)as the mobile *** acid was determined in UV at 365 nm and fluorescence at λ_(exc)325 nm with a 550 nm filter,*** effects of plants extracts on linoleic acid peroxidation were measured by an indirect in vitro colorimetric ***:Chromatographic resolution permitted reliable quantification in both measurement modes and calibration curves were linear in a range of 150-1000 ng/*** was found to contain significantly higher concentrations of rosmarinic *** densitometric quantification allowed the analysis of many samples in a short time with reasonable precision(total precision for Mentha spp extracts,5.1%and 5.8%for UV and fluorescence detection,respectively).The HPTLC data,allied to assays of linoleic acid peroxidation prevention,suggested the potential of ***(52%Trolox®equivalents)as a natural source for inhibitors of lipid ***:Densitometry can be used for routine determination and quality control of rosmarinic acid in herbal and formulations containing Mentha species.

关键词： Densitometry high performance thin layer chromatography Lipid peroxidation Mentha sp Rosmarinic acid

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Enhancement of Urinary Elimination of 3-Bromobenzanthrone Metabolites by Oral Supplementation of Ascorbic Acid in Guinea Pigs

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Biomedical and Environmental Sciences 2004年第4期17卷 390-396页

作者： RAVINDRA P. SINGH RAJ KHANNA SUBHASH K. KHANNA MUKUL DAS Department of Biochemistry Lucknow University Lucknow-226001Department of Biochemistry Lucknow University Lucknow-226001Food Toxicology Laboratory Industrial Toxicology Research Centre Postal Box No. 80 M. G. Marg Lucknow-226 001 IndiaFood Toxicology Laboratory Industrial Toxicology Research Centre Postal Box No. 80 M. G. Marg Lucknow-226 001 India

Objective 3-Bromobenzanthrone (3-BBA), an anthraquinone intermediate dye, is extensively used in textile industry. Since, our prior studies have shown that 3-BBA caused significant depletion of ascorbic acid (AsA) levels, the effect of exogenous supplementation of AsA on the urinary elimination of 3-BBA metabolites was investigated. Method Guinea pigs were treated with single oral dose of 3-BBA (50 mg/kg b. wt.) in groundnut oil while another group was treated with single oral dose of 3-BBA (50 mg/kg b. wt.) along with 3 day prior and post oral supplementation of AsA. Control groups were either treated with groundnut oil or AsA alone. Urine from individual animals was collected, extracted and analysed on HPTLC. Results The highest elimination of 3-BBA (75 mg) was found to be in 0-24 h urine fraction which decreased to 18 mg and 5 mg in the two subsequent 24 hourly fractions of urine. Exogenous supplementation of AsA increased the total urinary elimination of 3-BBA by almost 77%. A total of 10 fluorescent metabolites excluding the parent compound were eliminated in the urine of guinea pigs treated with 3-BBA. Densitometric scanning of chromatogram showed different peaks at Rf 0.18, 0.22, 0.27, 0.34, 0.40, 0.48, 0.56, 0.66, 0.72, 0.80, and 0.95 which were eliminated and marked as urinary metabolite 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, and 11 respectively. AsA not only significantly enhanced the elimination of 3-BBA metabolites but also modified the pattern of metabolites drastically in 0-6 h, 6-24 h and 24-48 h urine fractions. Conclusion These results indicate that AsA may be useful in protecting the toxicity of 3-BBA by fascilitating the urinary metabolite(s) excretion of 3-BBA.

关键词： Ascorbic acid 3-Bromobenzanthrone Metabolites high performance thin layer chromatography

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