检索结果-南通市图书馆

Transcriptome Analysis via RNA Sequencing Reveals the Molecular Mechanisms Underlying the hedera helix Response to High Temperature

引用

Phyton-International Journal of Experimental Botany 2022年第11期91卷 2403-2417页

作者： Ting Zhang Ping Li Jiali Wei Shanghai Botanical Garden Shanghai200231China Shanghai Engineering Research Center of Sustainable Plant Innovation Shanghai200231China Shanghai Key Laboratory of Bio-Energy Crops School of Life SciencesShanghai UniversityShanghai200444China

hedera helix is an evergreen ornamental plant that is resistant to cool but not high temperature and deserves to be further researched for improving its adaptability to heat stress.Two hedera helix cultivars,heat-tolerant(HT)‘Jessica’and heat-sensitive(HS)‘Shamrock’,were used for differences analyses of transcriptome.We detected 6179 differentially expressed genes(DEGs)and 5992 DEGs in‘Jessica’and‘Shamrock’to heat stress,respectively.Among these,1983 upregulated DEGs and 1400 downregulated DEGs were shared between both varieties,resulting in enhancement of various pathways such as biosynthesis of secondary metabolites,glyoxylate dicarboxylate metabolism,and protein processing in endoplasmic reticulum(PPER),RNA transport,respectively.Among the common downregulated DEGs,72 TFs in 25 gene families were found,including members of the MYB and MYBrelated families,the bHLH family,etc.In‘Jessica’(HT),634 unique up-regulated DEGs were identified,including genes associated with phenylpropanoid biosynthesis,starch and sucrose metabolism,biosynthesis of amino acids.Most upregulated TFs of HT were upregulated much more rapidly than those of HS in response to high temperature.Eleven TF-encoding genes were selected to verify the RNA sequencing data by qPCR.This study revealed the gene expression patterns of ivy in response to heat stress and the molecular basis of heat tolerance,which provided theoretical references for improving the heat tolerance of ivy.

关键词： hedera helix RNA-sequencing heat stress heat tolerance transcriptome

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Antihypertensive efficacy of extract of hedera helix in high salt-induced hypertensive Sprague-Dawley rats

引用

Asian Pacific Journal of Tropical Medicine 2018年第8期11卷 473-479页

作者： Umme Salma Taous Khan Abdul Jabbar Shah Cardiovascular Research Group Department of Pharmacy COMSATS University IslamabadAbbottabad campus

Objective: To explore the antihypertensive effect of extracts from the leaves of hedera helix(H. helix) on normotensive and hypertensive rats in-vivo followed by vasodilatory studies in-vitro.Methods: The crude methanolic extract was prepared and the activity directed fractionation was carried out. Spectrophotometric analysis of total phenolic and flavonoid content was also done. HPLC analysis was performed for the detection of hederacoside C. In-vivo blood pressure study was carried out in normotensive and high salt-induced hypertensive SpragueDawley rats. Isolated aortic tissues from rat and rabbit were used for in-vitro studies. The effects were recorded and analyzed through PowerL ab data acquisition system. Results: Crude extract of H. helix(1-30 mg/kg) decreased blood pressure to greater extent in high salt-induced hypertensive rats in-vivo compared to the normotensive [Max. fall(58.59±0.02) mm Hg vs.(67.53±3.07) mmH g]. The n-hexane, chloroform, ethyl acetate and aqueous fractions were also checked. These fractions were more effective in hypertensive rats. Aqueous fraction was more potent and n-hexane the least. In isolated rat aortic rings precontracted with phenylephrine, crude extract induced endothelium-dependent effect. The endothelium-dependent component of vasodilatory effect was ablated with L-NAME, and denudation of endothelium. The aqueous fraction was most potent vasodilator. In aortic rings from hypertensive rats, extract and fractions produced partial endothelium-independent effect which was not affected by pretreatment with L-NAME, indicating endothelium dysfunction in the hypertensive rats and suggesting additional vasodilatory mechanisms. In rabbit aorta, the extract and fractions also inhibited phenylephrine and high K^+-induced precontractions, and shifted Ca^(++) concentration–response curves. Conclusions: Our findings indicate that extract and fractions of H. helix are antihypertensive remedies, which is the outcome of vasodilatory effect. This vasodilatory effect is mediated through ni

关键词： hedera helix Antihypertensive Vasorelaxant Endothelium-dependent Nitric oxide Calcium channel blocker

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：