检索结果-南通市图书馆

Prediction of VIGS efficiency by the Sfold program and its reliability analysis in gossypium hirsutum

Science Bulletin 2016年第7期61卷 543-551页

作者： Xiaoyang Ge Jie Wu Chaojun Zhang Qianhua Wang Yuxia Hou Zuoren Yang Zhaoen Yang Zhenzhen Xu Ye Wang Lili Lu Xueyan Zhang Jinping Hua Fuguang Li State Key Laboratory of Cotton Biology Institute of Cotton Research Chinese Academy of Agricultural Sciences Department of Plant Genetics and Breeding Key Laboratory of Crop Heterosis and Utilization of Ministry of Education Beijing Key Laboratory of Crop Genetic Improvement China Agricultural University College of Science China Agricultural University

Genetic transformation in some plant species, including cotton （gossypium hirsutum）, is hampered by laborious and time-consuming processes and often unachievable. Virus-induced gene silencing （VIGS） by double-stranded RNAs can serve as a reverse-genetics tool to determine gene function. However, knockdown levels vary greatly when using a tobacco rattle virus-based vector that carries different cDNA fragments of a gene. How to choose the optional target fragment for high interference efficiency is very challenging. Addressing this challenge requires increasing the efficacy of small interference RNA （siRNA） in target fragment. Here, we describe a method to assess VIGS efficiency by comparing the following parameters of siRNA in target sequence： the disruptionenergy of the target （△Gdisruption）, the differential stability of siRNA duplex ends （DSSE）, and the internal stability at positions 9-14 of the siRNA antisense strand （AIS）, which are calculated by Sfold program （http：//***. org）. We find that the siRNAs with low mGdisruption, high DSSE and high AIS have high activity and easily result in high VIGS efficiency by experimentally testing the actual knockdown levels of the four target genes, GhPDS, GhCLA1, GhAOS1, and GhCXE1 via choosing different target sequences for each gene. Therefore, the Sfold pro- gram can be used to analyze target sequences when car- rying out VIGS design to increase gene-silencing effects in plants.

关键词： VIGS Sfold program siRNA Targetsequences gossypium hirsutum

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Genetic variation in LBL1 contributes to depth of leaf blades lobes between cotton subspecies, gossypium barbadense and gossypium hirsutum

引用

Journal of Integrative Agriculture 2018年第11期17卷 2394-2404页

作者： HE Dao-fang ZHAO Xiang LIANG Cheng-zhen ZHU Tao Muhammad Ali Abid CAI Yong-ping HE Jin-ling ZHANG Rui School of Life Science Anhui Agricultural University Biotechnology Research Institute Chinese Academy of Agricultural Sciences

Leaf is a essential part of the plants for photosynthetic activities which mainly economize the resources for boll heath. Significant variations of leaf shapes across the gossypium sp. considerably influence the infiltration of sunlight for photosynthesis. To understand the genetic variants and molecular processes underlying for cotton leaf shape, we used F2 population derived from upland cotton genotype P30A （shallow-lobed leaf） and sea-island cotton genotype ISR （deep-lobed leaf） to map leaf deep lobed phenotype controlling genes LBL1 and LBL2. Genetic analysis and localization results have unmasked the position and interaction between both loci of LBL1 and LBL2, and revealed the co-dominance impact of the genes in regulating depth of leaf blades lobes in cotton. LBL1 had been described as a main gene and member of transcription factor family leucine zipper （HD-ZIPI） from a class I homologous domain factor ***2G244000. The qRT-PCR results elaborated the continuous change in expression level of LBL1 at different growth stages and leaf parts of cotton. Higher expression level was observed in mature large leaves followed by medium and young leaves respectively. For further confirmation, plants were tested from hormonal induction treatments, which explained that LBL 1 expression was influenced by hormonal signaling. Moreover, the highest expression level was detected in brassinolides （BR） treatment as compared to other hormones, and this hormone plays an important role in the process of leaf blade lobed formation.

关键词： leaf blades lobes HD-ZIPI LBL1 cotton gossypium barbadense gossypium hirsutum

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Genome-Wide Identification of Genes Responsive to ABA and Cold/Salt Stresses in gossypium hirsutum by Data-Mining and Expression Pattern Analysis

引用

Agricultural Sciences in China 2011年第4期10卷 499-508页

作者： ZHU Long-fu HE Xin YUAN Dao-jun XU Lian XU Li TU Li-li SHEN Guo-xin ZHANG Hong ZHANG Xian-long National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan 430070 P.R.China Department of Biological Science Texas Tech University Lubbock 79409 USA

For making better use of nucleic acid resources of gossypium hirsutum, a data-mining method was used to identify putative genes responsive to various abiotic stresses in G. hirsutum. Based on the compiled database including genes involved in abiotic stress response in Arabidopsis thaliana and the comprehensive analysis tool of GENEVESTIGATOR v3, 826 genes up-regulated or down-regulated significantly in roots or leaves during salt or cold treatment in Arabidopsis were identified. As compared to these 826 Arabidopsis genes annotated, 38 homologous expressed sequence tags （ESTs） from G. hirsutum were selected randomly and their expression patterns were studied using a quantitative real-time reverse transcription-polymerase chain reaction method. Among these 38 ESTs, about 55% of the genes （21 of 38） were different in response to ABA between cotton and Arabidopsis, whereas 70% of genes had similar responses to cold and salt treatments, and some of them which had not been characterized in Arabidopsis are now being investigated in gene function studies. According to these results, this approach of analyzing ESTs appears effective in large-scale identification of cotton genes involved in abiotic stress and might be adopted to determine gene functions in various biologic processes in cotton.

关键词： cold stress salt stress data-mining gene gossypium hirsutum

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Overexpression of the phytochrome B gene from Arabidopsis thaliana increases plant growth and yield of cotton (gossypium hirsutum)

引用

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第4期12卷 326-334页

作者： Abdul Qayyum RAO Muhammad IRFAN Zafar SALEEM Idrees Ahmad NASIR Sheikh RIAZUDDIN Tayyab HUSNAIN National Centre of Excellence in Molecular Biology

The phytochrome B (PHYB) gene of Arabidopsis thaliana was introduced into cotton through Agrobacterium *** and expression of PHYB gene in cotton plants were confirmed by molecular *** RNA (mRNA) expression in one of the transgenic lines,QCC11,was much higher than those of control and other transgenic *** cotton plants showed more than a two-fold increase in photosynthetic rate and more than a four-fold increase in transpiration rate and stomatal *** increase in photosynthetic rate led to a 46% increase in relative growth rate and an 18% increase in net assimilation *** recorded up to two generations,both in the greenhouse and in the field,revealed that overexpression of Arabidopsis thaliana PHYB gene in transgenic cotton plants resulted in an increase in the production of cotton by improving the cotton plant growth,with 35% more ***,the presence of the Arabidopsis thaliana PHYB gene caused pleiotropic effects like semi-dwarfism,decrease in apical dominance,and increase in boll size.

关键词： Transformation gossypium hirsutum Phytochrome B Overexpression Plant growth Yield

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Genome-wide assessment of genetic diversity and fiber quality traits characterization in gossypium hirsutum races

引用

Journal of Integrative Agriculture 2017年第11期16卷 2402-2412页

作者： Kiflom Weldu Okubazghi LI Xiao-na CAI Xiao-yan WANG Xing-xing CHEN Hao-dong ZHOU Zhong-li WANG Chun-ying WANG Yu-hong LIU Fang WANG Kun-bo State Key Laboratory of Cotton Biology Institute of Cotton Research Chinese Academy of Agricultural Sciences Anyang 455000 P.R.China Hamelmalo Agricultural College Keren P. O. Box 397 Eritrea Cotton Sciences Research Institute of Hunan/National Hybrid Cotton Research Promotion Center Changde 415101 P.R. China

gossypium hirsutum races are believed to be potential reservoirs of desirable traits, which can play crucial roles to overcome the existing narrow genetic base of modern Upland cotton cultivars. However, prior to utilizing the races in cotton improve- ment programs, understanding their genetic constitutions is needed. Thus, this study used molecular and morphological techniques to characterize 110 G. hirsutum germplasm including 109 semi-wild accessions and one Upland cotton cultivar, CRI12. In the study, 104 SSR markers detected 795 alleles, with an average of 7.64 alleles per marker, ranging from 3 to 14, and average polymorphism information content （PIC） value of 0.71. And 96 of the markers were found to be highly informative, with PIC value〉0.50. Pairwise genetic similarity coefficient across the accessions ranged from 0.19 to 1.00, with an average value of 0.46. Morphological characterization was done using fiber length, fiber strength, micronaire, fiber uniformity index, and fiber elongation. Pairwise taxonomic distance within the accessions ranged from 0.17 to 3.41, with a mean of 1.33. The SSR and fiber quality traits data set based unweighted pair group method of arithmetic mean （UPGMA） analysis grouped the accessions into 7 and 12 distinct clusters, respectively, that corresponds well with the results of prin- cipal component analysis （PCA）. Our study revealed the existence of vast molecular and morphological diversities within the accessions and provided valuable information on each semi-wild accession for quick and better informed germplasm utilization in cotton breeding programs.

关键词： semi-wild accessions gossypium hirsutum SSR markers genetic similarity taxonomic distance

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The G-protein α subunit GhGPA positively regulates gossypium hirsutum resistance to Verticillium dahliae via induction of SA and JA signaling pathways and ROS accumulation

引用

The Crop Journal 2021年第4期9卷 823-833页

作者： Bin Chen Yan Zhang Jun Yang Man Zhang Qingming Ma Xingfen Wang Zhiying Ma State Key Laboratory of North China Crop Improvement and Regulation/Key Laboratory for Crop Germplasm Resources of Hebei Hebei Agricultural UniversityBaoding 071001HebeiChina

Verticillium wilt, a devastating disease in cotton caused by Verticillium dahliae, reduces cotton quality and yield. Heterotrimeric GTP-binding proteins, consisting of Ga, Gb, and Gc subunits, transducers of receptor signaling, function in a wide range of biological events. However, the function of Ga proteins in the regulation of defense responses in plants is largely unexplored, except for a few reports on model species. In the present study, a cotton G-protein a-subunit-encoding gene(GhGPA) was isolated from Verticillium wilt-resistant gossypium hirsutum(upland cotton) cv. ND601. GhGPA transcription was up-regulated under V. dahliae stress, with higher expression in tolerant than in susceptible cotton *** localization revealed GhGPA to be located in the plasma membrane. GhGPA shows high(85.0%) identity with Arabidopsis AT2 G26300(AtGPA1), and AtGPA1 gpa1-4 mutants displayed susceptibility to V. dahliae. Ectopic expression of GhGPA successfully restored the resistance of Arabidopsis gpa1-4 mutants to Verticillium wilt and made them more resistant than the wild type. Overexpression of GhGPA in Arabidopsis markedly increased the resistance and resulted in dramatic up-regulation of pathogenesis-related(PR) genes and increased in H2 O2 accumulation and salicylic acid(SA) and jasmonic acid(JA) contents. However, suppressing GhGPA expression via virus-induced gene silencing(VIGS)increased susceptibility to Verticillium wilt, down-regulated the expression of PR and marker genes in SA and JA signaling pathways, and reduced H2 O2 content. The contents of SA and JA in Arabidopsis gpa1-4 and VIGS cotton were lower than those in the wild type and empty-vector control. However,GhGPA-overexpressing Arabidopsis contained more SA and JA than the wild type when inoculated with V. dahliae. Thus, GhGPA plays a vital role in Verticillium wilt resistance by inducing SA and JA signaling pathways and regulating the production of reactive oxygen species. These findings

关键词： gossypium hirsutum GhGPA Verticillium wilt Pathogenesis-related genes ROS

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Isolation and Expression Analysis of Two Genes Encoding Cinnamate 4-Hydroxylase from Cotton(gossypium hirsutum)

引用

Journal of Integrative Agriculture 2014年第10期13卷 2102-2112页

作者： NI Zhi-yong LI Bo Neumann M Peter Lü Meng FAN Ling Institute of Nuclear and Biological Technologies Xinjiang Academy of Agricultural Sciences College of Agronomy Xinjiang Agricultural University Plant Physiology Laboratory Department of EnvironmentalWater and Agricultural EngineeringFaculty of Civil and Environmental EngineeringTechnion-Israel Institute of Technology

Two genes （GhC4H1 and GhC4H2） that encode putative cotton cinnamate 4-hydroxylases that catalyze the second step in the phenylpropanoid pathway were isolated from developing cotton fibers. GhC4H1 and GhC4H2 each contain open reading frames of 1 518 base pairs （bp） in length and both encode proteins consisting of 505 amino acid residues. They are 90.89% identical to each other at the amino acid sequence level and belong to class I of plant C4Hs. GhC4H1 and GhC4H2 genomic DNA are 2 247 and 2 161 bp long, respectively, and contain two introns located at conserved positions relative to the coding sequence. GhC4HI and GhC4H2 promoters were isolated and found to contain many cis-elements （boxes P, L and AC-1 element） previously identified in the promoters of other phenylpropanoid pathway genes. Histochemical staining showed GUS expression driven by the GhC4H1 and GhC4H2 promoters in ovules and fibers tissues. GhC4H1 and GhC4H2 were also widely expressed in other cotton tissues. GhC4H2 expression reached its highest level during the elongation stage of fiber development, whereas GhC4H1 expression increased during the secondary wall development period in cotton fibers. Our results contribute to a better understanding of the biochemical role of GhC4H1 and GhC4H2 in cotton fiber development.

关键词： cinnamate 4-hydroxylase gossypium hirsutum promoter analysis phenylpropanoid pathway

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Two farnesyl pyrophosphate synthases,GhFPS1–2,in gossypium hirsutum are involved in the biosynthesis of farnesol to attract parasitoid wasps

引用

Journal of Integrative Agriculture 2020年第9期19卷 2274-2285页

作者： ZHANG Hong HUANG Xin-zheng JING Wei-xia LIU Dan-feng Khalid Hussain DHILOO HAO Zhi-min ZHANG Yong-jun Key Laboratory of Hebei Province for Molecular Plant-Microbe Interaction College of Life SciencesHebei Agricultural UniversityBaoding 071000P.R.China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China Department of Entomology Faculty of Crop ProtectionSindh Agriculture UniversityTandojamHyderabad 70060Pakistan

Sesquiterpenoids play an import role in the direct or indirect defense of *** pyrophosphate synthases(FPSs)catalyze the biosynthesis of farnesyl pyrophosphate,which is a key precursor of farnesol and(E)-β-*** the current study,two FPS genes in gossypium hirsutum,GhFPS1 and GhFPS2,were heterologously cloned and functionally characterized in a greenhouse *** open reading frames for full-length GhFPS1 and GhFPS2 were each 1029 nucleotides,and encoded two proteins of 342 amino acids with molecular weights of 39.4 *** deduced amino acid sequences of GhFPS1–2 showed high identity to FPSs of other *** real-time PCR analysis revealed that GhFPS1 and GhFPS2 were highly expressed in *** leaves,and were upregulated in methyl jasmonate(MeJA)-,methyl salicylate(MeSA)-and aphid infestation-treated cotton *** recombinant proteins of either GhFPS1 or GhFPS2 plus calf intestinal alkaline phosphatase could convert geranyl diphosphate(GPP)or isopentenyl diphosphate(IPP)to one major product,***,in electrophysiological response and Y-tube olfactometer assays,farnesol showed obvious attractiveness to female Aphidius gifuensis,which is an important parasitic wasp of *** findings suggest that two GhFPSs are involved in farnesol biosynthesis and they play a crucial role in indirect defense of cotton against aphid infestation.

关键词： gossypium hirsutum farnesyl pyrophosphate synthase GhFPS1-2 biosynthesis of farnesol Aphidius gifuensis behavioral orientation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Investigation of GhFAT Genes Related to Seed Oil Content and Fatty Acid Composition in gossypium hirsutum L.

引用

Phyton-International Journal of Experimental Botany 2023年第5期92卷 1633-1647页

作者： Ziyang Feng Xinqi Cheng Tianwen Wang Yongchao Han Haihong Chen Xinyu Zhang Jie Sun Wei Zhang Feng Liu Key Laboratory of Oasis Eco-Agriculture College of AgricultureShihezi UniversityShihezi832000China College of Biology and Agricultural Resources Huanggang Normal UniversityHuanggang438000China College of Biology and Food Engineering Anhui Polytechnic UniversityWuhu241000China

Fatty Acyl-ACP thioesterase(FAT)is a key enzyme controlling oil biosynthesis in plant *** can be divided into two subfamilies,FATA and FATB according to their amino acid sequences and substrate *** Upland cotton genome contains 20 GhFAT genes,amongst which 6 genes were of the GhFATA subfamily and 14 of the GhFATB *** 20 GhFAT genes are unevenly distributed on 14 *** GhFATA genes have 5 or 7 exons and the GhFATB genes have 6 or 7 *** GhFAT proteins have the conserved Acyl-ACP_TE domain and PLN02370 super family,the typical characteristics of plant *** of the expression level of GhFATs and the compositions of fatty acid in 5-60 days-post-anthesis seeds showed that the ratio of saturated fatty acids to unsaturated fatty acids was consistent with the expression profile of GhFATB12,GhFATB3,and GhFATB10;the ratio of monounsaturated fatty acid to polyunsaturated fatty acids was consistent with the expression profile of *** oil contents of mature cottonseeds were positively correlated with the contents of palmitic acid and linolenic acid as well as seed *** results provide essential information for further exploring the role(s)of the specific GhFATs in determining oil biosynthesis and cottonseed compositions.

关键词： gossypium hirsutum fatty Acyl-ACP thioesterase biological information expression profile seed vigor

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Identification and Profiling of Known and Novel Fiber MicroRNAs during the Secondary Wall Thickening Stage in Cotton(gossypium hirsutum) via High-Throughput Sequencing

引用

Journal of Genetics and Genomics 2014年第10期41卷 553-556页

作者： Dingwei Yua Yanmei Wang Wei Xue Shuli Fan Shuxun Yu Jin-Yuan Liu Laboratory of Plant Molecular Biology Center for Plant Biology School of Life Sciences Tsinghua University State Key Laboratory of Cotton Biology Institute of Cotton Research Chinese Academy of Agricultural Sciences

Upland cotton （gossypium hirsutum L.） is an allotetraploid species originated from interspecific hybridization between AA-genome diploid （G. arboretum） and DD-genome diploid （G. raimondii）（Wendel et al., 1992）. Cotton fibers are single-celled trichomes that emerge from the ovule epidermal cells. Indexed by the number of days post-anthesis （dpa）, fiber morphogenesis includes four distinct but overlapping steps： initiation （0-3 dpa）, elongation （3-20 dpa）, secondary cell wall thickening （15-45 dpa） and maturation （40-60 dpa）（Yang et al., 2008, Du et al., 2013）. The efficiency and duration of each morphogenesis stage is important to the quality attributes of the mature fiber. Cell elongation is critical for fiber length, whereas secondary cell wall thickening is important for fiber fineness and strength （Meinert and Delmer, 1977）.

关键词： SWT gossypium hirsutum via High-Throughput Sequencing Identification and Profiling of Known and Novel Fiber MicroRNAs during the Secondary Wall Thickening Stage in Cotton RNA

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：