Genetic variation in LBL1 contributes to depth of leaf blades lobes between cotton subspecies, Gossypium barbadense and Gossypium hirsutum

作     者：HE Dao-fang ZHAO Xiang LIANG Cheng-zhen ZHU Tao Muhammad Ali Abid CAI Yong-ping HE Jin-ling ZHANG Rui 

作者机构：School of Life Science Anhui Agricultural University Biotechnology Research Institute Chinese Academy of Agricultural Sciences 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2018年第17卷第11期

页      面：2394-2404页

核心收录：

学科分类：0710[理学-生物学] 0832[工学-食品科学与工程（可授工学、农学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 0713[理学-生态学] 

基　　金：supported by the Genetically Modified Organisms Breeding Major Projects,China (2016ZX0800 5004, 2016ZX08009003-003-004) the National Natural Science Foundation of China (31601349) the Innovation Program of Chinese Academy of Agricultural Sciences 

主　　题：leaf blades lobes HD-ZIPI LBL1 cotton Gossypium barbadense Gossypium hirsutum 

摘      要：Leaf is a essential part of the plants for photosynthetic activities which mainly economize the resources for boll heath. Significant variations of leaf shapes across the Gossypium sp. considerably influence the infiltration of sunlight for photosynthesis. To understand the genetic variants and molecular processes underlying for cotton leaf shape, we used F2 population derived from upland cotton genotype P30A （shallow-lobed leaf） and sea-island cotton genotype ISR （deep-lobed leaf） to map leaf deep lobed phenotype controlling genes LBL1 and LBL2. Genetic analysis and localization results have unmasked the position and interaction between both loci of LBL1 and LBL2, and revealed the co-dominance impact of the genes in regulating depth of leaf blades lobes in cotton. LBL1 had been described as a main gene and member of transcription factor family leucine zipper （HD-ZIPI） from a class I homologous domain factor ***2G244000. The qRT-PCR results elaborated the continuous change in expression level of LBL1 at different growth stages and leaf parts of cotton. Higher expression level was observed in mature large leaves followed by medium and young leaves respectively. For further confirmation, plants were tested from hormonal induction treatments, which explained that LBL 1 expression was influenced by hormonal signaling. Moreover, the highest expression level was detected in brassinolides （BR） treatment as compared to other hormones, and this hormone plays an important role in the process of leaf blade lobed formation.