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Dynamic profiles of DNA methylation and the interaction with histone acetylation during fiber cell initiation of gossypium hirsutum

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Journal of Cotton Research 2022年第1期5卷 34-47页

作者： CHEN Guangyu LI Yonghui WEI Zhenzhen GAN Lei LIU Jisheng WANG Zhi Zhengzhou Research Base State Key Laboratory of Cotton BiologySchool of Agricultural SciencesZhengzhou UniversityZhengzhou 450001China Institute of Cotton Research Chinese Agricultural Academy of SciencesAnyang 455000China

Background:Fiber,as the main product of cotton,provides main raw material for the textile *** key factors have been revealed a significant role in fiber cell development including Myb proteins,phytohormones,fatty acid metabolites,and epigenetic *** methylation is one of the important epigenetic modifications to regulate plant development and responses to abiotic or biotic *** general,DNA methylation consisting of 5mC and 6mA regulates the chromatin structure and gene transcription to affect plant development,however,the detailed role and underlying mechanism of DNA methylation in the fiber development of cotton are yet ***:Here,systematical study of the 5mC and 6mA DNA methylation profiles during the fiber initiation period of Xu142 and its glabrous mutant Xu142fl represented a clear alteration of global DNA methylation associated with fiber cell ***,the genome-wide identification of genes responsible for methylation regulation at the fifth carbon of cytosine and the sixth carbon of adenine of DNA was operated in gossypium *** a result,13,10,6,and 17 genes were identified for 5mC methylation,5mC demethylation,6mA methylation,and 6mA demethylation,*** then investigated the tissue expression pattern of all these genes,and some genes showed higher expression levels in fiber initiation,among which some displayed a significant change in transcription between Xu142 and *** possible interaction between histone acetylation and DNA methylation in fiber initiation through in vitro culture was studied by dot blot,and the results showed that repressed histone deacetylation by Trichostatin A(TSA)inhibited the global DNA methylation,and some causal genes(e.g.,GhDMT13,GhDAMT2,GhALKBH12,GhDM7)were also ***:In this study,all the findings indicated the interplay between histone acetylation and DNA methylation,supporting their important roles and providing precious clues for the epigene

关键词： Fiber initiation DNA methylation Histone acetylation gossypium hirsutum

Characters and structures of the nucleobase-ascorbate transporters(NAT)family genes in gossypium hirsutum and their roles in responding to salt and drought stresses

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Journal of Cotton Research 2022年第2期5卷 115-129页

作者： GUO Lixue ZHAO Lanjie LU Xuke CHEN Xiugui WANG Shuai WANG Junjuan WANG Delong YIN Zujun CHEN Chao FAN Yapeng ZHANG Yuexin YE Wuwei State Key Laboratory of C otton Biology/I nstitute of Cott on Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base School of Agricultural SciencesZhengzhou University/Key Laboratory for Cotton Genetic ImprovementMOARAAnyang 455000HenanChina

Background: Nucleobase-ascorbate transporters(NAT), synonymously called nucleobase-cation symporter 2(NCS2) proteins, were earlier reported to be involved in plant growth, development and resistance to stress. Previous studies concluded that s a polymorphic SNP associated with NAT12 was significant di erent between salt-tolerant and salt-sensitive materials of upland cotton. In current study, a comprehensive analysis of NAT family genes was conducted for the first time in ***: In this study, we discovered 32, 32, 18, and 16 NAT genes in gossypium hirsutum, G. barbadense, G. raimondii and G. arboreum, respectively, which were classified into four groups(groups I–IV) based on the multiple sequence analysis. These GhNAT genes were unevenly distributed on At and Dt sub-genome in G. hirsutum. Most GhNAT members in the same group had similar gene structure characteristics and motif composition. The collinearity analysis revealed segmental duplication as well as tandem duplication contributing to the expansion of the GhNATs. The analysis of cis-acting regulatory elements of GhNATs showed that the function of GhNAT genes in cotton might be related to plant hormone and stress response. Under di erent conditions, the expression levels further suggested the GhNAT family genes were associated with plant response to various abiotic stresses. GhNAT12 was detected in the plasma membrane. And it was validated that the GhNAT12 gene played an important role in regulating cotton resistance to salt and drought stress through the virus-induced gene silencing(VIGS) ***: A comprehensive analysis of NAT gene family was performed in cotton, including phylogenetic analysis, chromosomal location, collinearity analysis, motifs, gene structure and so on. Our results will further broaden the insight into the evolution and potential functions of NAT genes in cotton. Current findings could make significant contribution towards screening more candidate genes related to b

关键词： gossypium hirsutum NAT gene family VIGS Salt and drought stresses

Genetic variation in LBL1 contributes to depth of leaf blades lobes between cotton subspecies, gossypium barbadense and gossypium hirsutum

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Journal of Integrative Agriculture 2018年第11期17卷 2394-2404页

作者： HE Dao-fang ZHAO Xiang LIANG Cheng-zhen ZHU Tao Muhammad Ali Abid CAI Yong-ping HE Jin-ling ZHANG Rui School of Life Science Anhui Agricultural University Biotechnology Research Institute Chinese Academy of Agricultural Sciences

Leaf is a essential part of the plants for photosynthetic activities which mainly economize the resources for boll heath. Significant variations of leaf shapes across the gossypium sp. considerably influence the infiltration of sunlight for photosynthesis. To understand the genetic variants and molecular processes underlying for cotton leaf shape, we used F2 population derived from upland cotton genotype P30A （shallow-lobed leaf） and sea-island cotton genotype ISR （deep-lobed leaf） to map leaf deep lobed phenotype controlling genes LBL1 and LBL2. Genetic analysis and localization results have unmasked the position and interaction between both loci of LBL1 and LBL2, and revealed the co-dominance impact of the genes in regulating depth of leaf blades lobes in cotton. LBL1 had been described as a main gene and member of transcription factor family leucine zipper （HD-ZIPI） from a class I homologous domain factor ***2G244000. The qRT-PCR results elaborated the continuous change in expression level of LBL1 at different growth stages and leaf parts of cotton. Higher expression level was observed in mature large leaves followed by medium and young leaves respectively. For further confirmation, plants were tested from hormonal induction treatments, which explained that LBL 1 expression was influenced by hormonal signaling. Moreover, the highest expression level was detected in brassinolides （BR） treatment as compared to other hormones, and this hormone plays an important role in the process of leaf blade lobed formation.

关键词： leaf blades lobes HD-ZIPI LBL1 cotton gossypium barbadense gossypium hirsutum

Overexpression of the phytochrome B gene from Arabidopsis thaliana increases plant growth and yield of cotton (gossypium hirsutum)

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第4期12卷 326-334页

作者： Abdul Qayyum RAO Muhammad IRFAN Zafar SALEEM Idrees Ahmad NASIR Sheikh RIAZUDDIN Tayyab HUSNAIN National Centre of Excellence in Molecular Biology

The phytochrome B (PHYB) gene of Arabidopsis thaliana was introduced into cotton through Agrobacterium *** and expression of PHYB gene in cotton plants were confirmed by molecular *** RNA (mRNA) expression in one of the transgenic lines,QCC11,was much higher than those of control and other transgenic *** cotton plants showed more than a two-fold increase in photosynthetic rate and more than a four-fold increase in transpiration rate and stomatal *** increase in photosynthetic rate led to a 46% increase in relative growth rate and an 18% increase in net assimilation *** recorded up to two generations,both in the greenhouse and in the field,revealed that overexpression of Arabidopsis thaliana PHYB gene in transgenic cotton plants resulted in an increase in the production of cotton by improving the cotton plant growth,with 35% more ***,the presence of the Arabidopsis thaliana PHYB gene caused pleiotropic effects like semi-dwarfism,decrease in apical dominance,and increase in boll size.

关键词： Transformation gossypium hirsutum Phytochrome B Overexpression Plant growth Yield

Genome-wide assessment of genetic diversity and fiber quality traits characterization in gossypium hirsutum races

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Journal of Integrative Agriculture 2017年第11期16卷 2402-2412页

作者： Kiflom Weldu Okubazghi LI Xiao-na CAI Xiao-yan WANG Xing-xing CHEN Hao-dong ZHOU Zhong-li WANG Chun-ying WANG Yu-hong LIU Fang WANG Kun-bo State Key Laboratory of Cotton Biology Institute of Cotton Research Chinese Academy of Agricultural Sciences Anyang 455000 P.R.China Hamelmalo Agricultural College Keren P. O. Box 397 Eritrea Cotton Sciences Research Institute of Hunan/National Hybrid Cotton Research Promotion Center Changde 415101 P.R. China

gossypium hirsutum races are believed to be potential reservoirs of desirable traits, which can play crucial roles to overcome the existing narrow genetic base of modern Upland cotton cultivars. However, prior to utilizing the races in cotton improve- ment programs, understanding their genetic constitutions is needed. Thus, this study used molecular and morphological techniques to characterize 110 G. hirsutum germplasm including 109 semi-wild accessions and one Upland cotton cultivar, CRI12. In the study, 104 SSR markers detected 795 alleles, with an average of 7.64 alleles per marker, ranging from 3 to 14, and average polymorphism information content （PIC） value of 0.71. And 96 of the markers were found to be highly informative, with PIC value〉0.50. Pairwise genetic similarity coefficient across the accessions ranged from 0.19 to 1.00, with an average value of 0.46. Morphological characterization was done using fiber length, fiber strength, micronaire, fiber uniformity index, and fiber elongation. Pairwise taxonomic distance within the accessions ranged from 0.17 to 3.41, with a mean of 1.33. The SSR and fiber quality traits data set based unweighted pair group method of arithmetic mean （UPGMA） analysis grouped the accessions into 7 and 12 distinct clusters, respectively, that corresponds well with the results of prin- cipal component analysis （PCA）. Our study revealed the existence of vast molecular and morphological diversities within the accessions and provided valuable information on each semi-wild accession for quick and better informed germplasm utilization in cotton breeding programs.

关键词： semi-wild accessions gossypium hirsutum SSR markers genetic similarity taxonomic distance

Genome-Wide Identification of Genes Responsive to ABA and Cold/Salt Stresses in gossypium hirsutum by Data-Mining and Expression Pattern Analysis

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Agricultural Sciences in China 2011年第4期10卷 499-508页

作者： ZHU Long-fu HE Xin YUAN Dao-jun XU Lian XU Li TU Li-li SHEN Guo-xin ZHANG Hong ZHANG Xian-long National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan 430070 P.R.China Department of Biological Science Texas Tech University Lubbock 79409 USA

For making better use of nucleic acid resources of gossypium hirsutum, a data-mining method was used to identify putative genes responsive to various abiotic stresses in G. hirsutum. Based on the compiled database including genes involved in abiotic stress response in Arabidopsis thaliana and the comprehensive analysis tool of GENEVESTIGATOR v3, 826 genes up-regulated or down-regulated significantly in roots or leaves during salt or cold treatment in Arabidopsis were identified. As compared to these 826 Arabidopsis genes annotated, 38 homologous expressed sequence tags （ESTs） from G. hirsutum were selected randomly and their expression patterns were studied using a quantitative real-time reverse transcription-polymerase chain reaction method. Among these 38 ESTs, about 55% of the genes （21 of 38） were different in response to ABA between cotton and Arabidopsis, whereas 70% of genes had similar responses to cold and salt treatments, and some of them which had not been characterized in Arabidopsis are now being investigated in gene function studies. According to these results, this approach of analyzing ESTs appears effective in large-scale identification of cotton genes involved in abiotic stress and might be adopted to determine gene functions in various biologic processes in cotton.

关键词： cold stress salt stress data-mining gene gossypium hirsutum

The G-protein α subunit GhGPA positively regulates gossypium hirsutum resistance to Verticillium dahliae via induction of SA and JA signaling pathways and ROS accumulation

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The Crop Journal 2021年第4期9卷 823-833页

作者： Bin Chen Yan Zhang Jun Yang Man Zhang Qingming Ma Xingfen Wang Zhiying Ma State Key Laboratory of North China Crop Improvement and Regulation/Key Laboratory for Crop Germplasm Resources of Hebei Hebei Agricultural UniversityBaoding 071001HebeiChina

Verticillium wilt, a devastating disease in cotton caused by Verticillium dahliae, reduces cotton quality and yield. Heterotrimeric GTP-binding proteins, consisting of Ga, Gb, and Gc subunits, transducers of receptor signaling, function in a wide range of biological events. However, the function of Ga proteins in the regulation of defense responses in plants is largely unexplored, except for a few reports on model species. In the present study, a cotton G-protein a-subunit-encoding gene(GhGPA) was isolated from Verticillium wilt-resistant gossypium hirsutum(upland cotton) cv. ND601. GhGPA transcription was up-regulated under V. dahliae stress, with higher expression in tolerant than in susceptible cotton *** localization revealed GhGPA to be located in the plasma membrane. GhGPA shows high(85.0%) identity with Arabidopsis AT2 G26300(AtGPA1), and AtGPA1 gpa1-4 mutants displayed susceptibility to V. dahliae. Ectopic expression of GhGPA successfully restored the resistance of Arabidopsis gpa1-4 mutants to Verticillium wilt and made them more resistant than the wild type. Overexpression of GhGPA in Arabidopsis markedly increased the resistance and resulted in dramatic up-regulation of pathogenesis-related(PR) genes and increased in H2 O2 accumulation and salicylic acid(SA) and jasmonic acid(JA) contents. However, suppressing GhGPA expression via virus-induced gene silencing(VIGS)increased susceptibility to Verticillium wilt, down-regulated the expression of PR and marker genes in SA and JA signaling pathways, and reduced H2 O2 content. The contents of SA and JA in Arabidopsis gpa1-4 and VIGS cotton were lower than those in the wild type and empty-vector control. However,GhGPA-overexpressing Arabidopsis contained more SA and JA than the wild type when inoculated with V. dahliae. Thus, GhGPA plays a vital role in Verticillium wilt resistance by inducing SA and JA signaling pathways and regulating the production of reactive oxygen species. These findings

关键词： gossypium hirsutum GhGPA Verticillium wilt Pathogenesis-related genes ROS

Isolation and Expression Analysis of Two Genes Encoding Cinnamate 4-Hydroxylase from Cotton(gossypium hirsutum)

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Journal of Integrative Agriculture 2014年第10期13卷 2102-2112页

作者： NI Zhi-yong LI Bo Neumann M Peter Lü Meng FAN Ling Institute of Nuclear and Biological Technologies Xinjiang Academy of Agricultural Sciences College of Agronomy Xinjiang Agricultural University Plant Physiology Laboratory Department of EnvironmentalWater and Agricultural EngineeringFaculty of Civil and Environmental EngineeringTechnion-Israel Institute of Technology

Two genes （GhC4H1 and GhC4H2） that encode putative cotton cinnamate 4-hydroxylases that catalyze the second step in the phenylpropanoid pathway were isolated from developing cotton fibers. GhC4H1 and GhC4H2 each contain open reading frames of 1 518 base pairs （bp） in length and both encode proteins consisting of 505 amino acid residues. They are 90.89% identical to each other at the amino acid sequence level and belong to class I of plant C4Hs. GhC4H1 and GhC4H2 genomic DNA are 2 247 and 2 161 bp long, respectively, and contain two introns located at conserved positions relative to the coding sequence. GhC4HI and GhC4H2 promoters were isolated and found to contain many cis-elements （boxes P, L and AC-1 element） previously identified in the promoters of other phenylpropanoid pathway genes. Histochemical staining showed GUS expression driven by the GhC4H1 and GhC4H2 promoters in ovules and fibers tissues. GhC4H1 and GhC4H2 were also widely expressed in other cotton tissues. GhC4H2 expression reached its highest level during the elongation stage of fiber development, whereas GhC4H1 expression increased during the secondary wall development period in cotton fibers. Our results contribute to a better understanding of the biochemical role of GhC4H1 and GhC4H2 in cotton fiber development.

关键词： cinnamate 4-hydroxylase gossypium hirsutum promoter analysis phenylpropanoid pathway

Two farnesyl pyrophosphate synthases,GhFPS1–2,in gossypium hirsutum are involved in the biosynthesis of farnesol to attract parasitoid wasps

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Journal of Integrative Agriculture 2020年第9期19卷 2274-2285页

作者： ZHANG Hong HUANG Xin-zheng JING Wei-xia LIU Dan-feng Khalid Hussain DHILOO HAO Zhi-min ZHANG Yong-jun Key Laboratory of Hebei Province for Molecular Plant-Microbe Interaction College of Life SciencesHebei Agricultural UniversityBaoding 071000P.R.China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China Department of Entomology Faculty of Crop ProtectionSindh Agriculture UniversityTandojamHyderabad 70060Pakistan

Sesquiterpenoids play an import role in the direct or indirect defense of *** pyrophosphate synthases(FPSs)catalyze the biosynthesis of farnesyl pyrophosphate,which is a key precursor of farnesol and(E)-β-*** the current study,two FPS genes in gossypium hirsutum,GhFPS1 and GhFPS2,were heterologously cloned and functionally characterized in a greenhouse *** open reading frames for full-length GhFPS1 and GhFPS2 were each 1029 nucleotides,and encoded two proteins of 342 amino acids with molecular weights of 39.4 *** deduced amino acid sequences of GhFPS1–2 showed high identity to FPSs of other *** real-time PCR analysis revealed that GhFPS1 and GhFPS2 were highly expressed in *** leaves,and were upregulated in methyl jasmonate(MeJA)-,methyl salicylate(MeSA)-and aphid infestation-treated cotton *** recombinant proteins of either GhFPS1 or GhFPS2 plus calf intestinal alkaline phosphatase could convert geranyl diphosphate(GPP)or isopentenyl diphosphate(IPP)to one major product,***,in electrophysiological response and Y-tube olfactometer assays,farnesol showed obvious attractiveness to female Aphidius gifuensis,which is an important parasitic wasp of *** findings suggest that two GhFPSs are involved in farnesol biosynthesis and they play a crucial role in indirect defense of cotton against aphid infestation.

关键词： gossypium hirsutum farnesyl pyrophosphate synthase GhFPS1-2 biosynthesis of farnesol Aphidius gifuensis behavioral orientation