检索结果-南通市图书馆

genomic structure analysis of SNC6, a progesterone-receptor associated protein gene, and cloning and characterization of its 5＇-flanking region

引用

Journal of Zhejiang University Science 2002年第1期3卷 100-105页

作者：曹江郑树叶景佳耿礼义方永明 CancerInstitute SecondAffdiatedHospitalCollgeofMedicineZhefiangUniversityHangzhou310009China

Objective: To analyze the genomic structure of SNC6, a progesterone\|receptor associated protein gene and its regulatory elements in its 5'\|flanking region. Methods: genomic sequence from GenBank database (accession number: Z98048) covering the whole SNC6 gene was used to analyze the genomic structure of SNC6 and design primers for PCR amplification of its 5'\|flanking region. A 1894 bp fragment of the 5'\|flanking region \{(-1814\} to +75) was cloned by PCR using genomic DNA from a healthy donor peripheral blood lymphocyte as template. This fragment, as well as 3 shorter derivative fragments (1423 bp, 632 bp and 416 bp, which correspond to -1344 to +75, -552 to +75 and -337 to +75 respectively), were subcloned into pGL2 series luciferase reporter vectors. These constructs were introduced into colorectal cancer cell line SW620 for transient expression of reporter gene and luciferase activities were measured. Results: The genomic structure analysis showed there are 12 exons for SNC6 gene, which spans 32017 bp (nt71529 to nt39513 in Z98048 sequence). All transfected SW620 cells with the above 5\|flanking region\|containing constructs showed luciferase activities. The highest luciferase activities were measured in transfected cells with vectors containing 1894 bp fragments, and the lowest luciferase activities were measured in transfected cells with vectors containing 416 bp fragments. Luciferase activities were higher in transfected cells with vectors containing 632 bp fragments than that in transfected cells with vectors containing 1423 bp fragments. Conclusion: The basic transcription\|promoting element (promoter) for SNC6 expression resides between 0 to -337, and two transcription\|enhancing elements (enhancer) resides between -337 to -552 and -1344 to -1814, whereas one transcription\|inhibiting element (silencer) exists between -552 to -1344.

关键词： SNC6 gene genomic structure 5'\|flanking region promoter luciferase assay

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

genomic structure analysis of SNC6, a progesterone-receptor associated protein gene, and cloning and characterization of its 5'-flanking region

引用

Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2002年第1期 101-106页

作者：曹江郑树叶景佳耿礼义方永明

Objective: To analyze the genomic structure of SNC6, a progesterone\|receptor associated protein gene and its regulatory elements in its 5’\|flanking region. Methods: genomic sequence from GenBank database (accession number: Z98048) covering the whole SNC6 gene was used to analyze the genomic structure of SNC6 and design primers for PCR amplification of its 5’\|flanking region. A 1894 bp fragment of the 5’\|flanking region \{(-1814\} to +75) was cloned by PCR using genomic DNA from a healthy donor peripheral blood lymphocyte as template. This fragment, as well as 3 shorter derivative fragments (1423 bp, 632 bp and 416 bp, which correspond to -1344 to +75, -552 to +75 and -337 to +75 respectively), were subcloned into pGL2 series luciferase reporter vectors. These constructs were introduced into colorectal cancer cell line SW620 for transient expression of reporter gene and luciferase activities were measured. Results: The genomic structure analysis showed there are 12 exons for SNC6 gene, which spans 32017 bp (nt71529 to nt39513 in Z98048 sequence). All transfected SW620 cells with the above 5\|flanking region\|containing constructs showed luciferase activities. The highest luciferase activities were measured in transfected cells with vectors containing 1894 bp fragments, and the lowest luciferase activities were measured in transfected cells with vectors containing 416 bp fragments. Luciferase activities were higher in transfected cells with vectors containing 632 bp fragments than that in transfected cells with vectors containing 1423 bp fragments. Conclusion: The basic transcription\|promoting element (promoter) for SNC6 expression resides between 0 to -337, and two transcription\|enhancing elements (enhancer) resides between -337 to -552 and -1344 to -1814, whereas one transcription\|inhibiting element (silencer) exists between -552 to -1344.

关键词： SNC6 gene genomic structure 5’\|flanking region promoter luciferase assay

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Genome-wide evolution of MAPKs family and their expression in response to bacterial infection in seahorse Hippocampus erectus

引用

Journal of Oceanology and Limnology 2021年第6期39卷 2309-2321页

作者： Kai WANG Xin WANG Qiang ZOU Han JIANG Rongrong ZHANG Yanan TIAN Lele ZHANG Qiang LIN School of Agriculture Ludong UniversityYantai 264025China CAS Key Laboratory of Tropical Marine Bio-resources and Ecology South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou) Guangzhou 511458China Yantai Branch of Shandong Technology Transfer Center Chinese Academy of SciencesYantai 264003China University of Chinese Academy of Sciences Beijing 100049China

Seahorses have evolved many unique biological traits,including a male brood pouch,the absence of caudal and pelvic fins,and the lack of spleen and gut-associated lymphatic *** mitogenactivated protein kinases(MAPKs)are known to be involved in various important biological processes including growth,differentiation,immunity,and stress ***,we hypothesized that the adaptive evolution and expression of the MAPK gene family in seahorse may differ from those of other teleost *** identified positive selection sites in the erk2,erk5,jnk1,and p38αMAPK genes of the lined seahorse Hippocampus erectus and tiger-tailed seahorse Hippocampus comes.A novel expression profile of MAPK cascade genes was found in seahorse larvae during the first day after birth based on the RNA-seq data of ***,which refl ected vital signs of immune response to its parental immune *** expression patterns of the four positively selected MAPK genes were analyzed following the bacterial challenge of Vibrio fortis,revealing their upregulation pattern in brood pouch and other immune *** study enriched our knowledge of the evolution of the *** MAPK subfamilies,and could help better understanding the functional role of MAPKs in teleosts.

关键词： mitogen-activated protein kinases(MAPKs) Hippocampus erectus genomic structure positive selection immune regulation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The silkworm homolog of Methoprene-tolerant （Met） gene reveals sequence conservation but function divergence

引用

Insect Science 2010年第4期17卷 313-324页

作者： Zhi-Qing Li Dao-Jun Cheng Ling Wei Ping Zhao Xu Shu Lin Tang Zhong-Huai Xiang Qing-You Xia The Key Sericultural Laboratory of Agricultural Ministry Southwest University Chongqing School of Life Science Southwest University Chongqing The Institute of Agronomy and Life Sciences Chongqing University Chongqing China

Methoprene-tolerant （Met） gene has been found to be involved in juvenile hormone （JH） action in insects. Herein, we isolated a silkworm （Bombyx mori） homolog of Met gene from Drosophila melanogaster using bio-informatics analysis and rapid amplification of cDNA ends - polymerase chain reaction method, and defined it as BmMet. The full-length cDNA ofBmMet gene consists of 1 917 nucleotides and includes a 1 368 bp of open reading frame for a deduced protein of 455 amino acids. All deduced protein sequences from Met genes in B. mori and other surveyed insects contain four typical domains ofbHLH, PAS-A, PAS-B and PAC, highlighting a high sequence conservation of Met genes during insect evolution. Also, genomic structure and phylogenic analysis suggested that Met in both B. mori and Drosophila species may originate from an ancestor gene with gce, another member of bHLH-PAS family, via gene duplication. In addition, BmMet was detected in all surveyed tissues and throughout the whole life of silkworm at transcriptional levels. Furthermore, silkworm individuals with RNAi silencing of BmMet gene in the early stage of the fourth instar larvae could molt normally and pupate successfully. This result was different from the observation in T. castaneum but similar to that in D. melanogaster after Met knockdown, revealing that the action mode of Met in B. mori and D. melanogaster should be divergent with that in other insect species.

关键词： Bombyx mori expression pattern genomic structure Methoprene-tolerantgene RNAi

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genetic Characteristics and Antimicrobial Susceptibility of Arcobacter butzleri Isolates from Raw Chicken Meat and Patients with Diarrhea in China

引用

Biomedical and Environmental Sciences 2021年第12期34卷 1024-1028页

作者： WANG Yuan Yuan ZHOU Gui Lan LI Ying GU Yi Xin HE Mu ZHANG Shuang JI Guo Qiang YANG Jie WANG Miao MA Hong Mei ZHANG Mao Jun Microbiology Laboratory Shunyi District Center for Disease Control and PreventionBeijing 101399China State Key Laboratory of Infectious Disease Prevention and Control Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesNational Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China

Arcobacter is an emerging foodborne pathogen *** this study,the prevalence,antimicrobial susceptibility and genetic characteristics of Arcobacter from different sources were *** *** isolates were obtained from 60 raw chicken meat samples(16/60,27%)and 150 patients with diarrhea(2/150,1.3%).The resistance ratios to nalidixic acid,ciprofloxacin,clindamycin,chloramphenicol,and florfenicol were 83.33%(15/18),38.89%(7/18),38.89%(7/18),33.33%(6/18)and 33.33%(6/18),*** performed whole genome sequencing of the 18 isolates,and we predicted antibiotic resistance genes and virulence factors by using assembled genomes through blastx *** resistance genes,blaOXA-464 and tet(H),and the C254T mutation in gyrA,were identified in the genomes of some resistant ***,virulence genes,such as flgG,flhA,flhB,fliI,fliP,motA,cadF,cjl349,ciaB,mviN,pldA and tlyA,were found in all strains,whereas hecA,hecB and iroE were found in only some *** tree analysis of *** isolates on the basis of the core-genome single nucleotide polymorphisms showed that two isolates from patients with diarrhea clustered together,separately from the isolates from raw chicken and the chicken *** study is the first comprehensive analysis of Arcobacter isolated in Beijing.

关键词： Arcobacter Arcobacter butzleri Prevalence Whole genome sequence Antimicrobial susceptibility genomic structure

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The Dicistroviridae: An Emerging Family of Invertebrate Viruses

引用

Virologica Sinica 2009年第5期24卷 415-427页

作者： Bryony C. Bonning Department of Entomology Iowa State University 418 Science Ⅱ Ames IA 50011-3222 USA

Dicistroviruses comprise a newly characterized and rapidly expanding family of small RNA viruses of invertebrates. Several features of this virus group have attracted considerable research interest in recent years. In this review I provide an overview of the Dicistroviridae and describe progress made toward the understanding and practical application of dicistroviruses, including (i) construction of the first infectious clone of a dicistrovirus, (ii) use of the baculovirus expression system for production of an infectious dicistrovirus, (iii) the use of Drosophila C virus for analysis of host response to virus infection, and (iv) correlation of the presence of Israeli acute paralysis virus with honey bee colony collapse disorder. The potential use of dicistroviruses for insect pest management is also discussed. The structure, mechanism and practical use of the internal ribosome entry site (IRES) elements has recently been reviewed elsewhere.

关键词： Taxonomy Virion structure Disease and ecology genomic structure

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Complete mitochondrial genome of the atlas moth,Attacus atlas(Lepidoptera:Saturniidae) and the phylogenetic relationship of Saturniidae species

Complete mitochondrial genome of the atlas moth,Attacus atla...

引用

中国蚕学会第九届青年学术研讨会

作者： Miao-Miao Chen Yan Li Mo Chen Huan Wang Qun Li Run-Xi Xia Cai-Yun Zeng Yu-Ping Li 刘彦群 Li Qin Insect Resource Center for Engineering and Technology of Liaoning Province Shenyang Agricultural University Administration Bureau of Xishuangbanna National Nature Reserve Key Laboratory of Wild Silkworms of Liaoning Province Sericultural Institute of Liaoning Province

Mitochondrial genome(mitogenome) can provide information for genomic structure as well as for phylogenetic analysis and evolutionary *** this study,we present the complete mitogenome of the atlas moth,Attacus atlas(Lepidoptera:Saturniidae),a well-known silk-producing and ornamental insect with the largest wing surface area of all *** mitogenome of *** is a circular molecule of 15,282 bp long,and its nucleotide composition shows heavily biased towards As and Ts,accounting for 79.30%.This genome comprises 13 protein-coding genes(PCGs),two ribosomal RNA genes(rRNAs),22 transfer RNA genes(tRNAs),and an A+T-rich *** is of note that this genome exhibits a slightly positive AT skew,which is different from the other known Saturniidae *** PCGs are initiated by ATN codons,except for COI with CGA *** six PCGs use a common stop codon of TAA or TAG,whereas the remaining seven use an incomplete termination codon T or *** tRNAs have the typical clover-leaf structure,with an exception for tRNA(AGN).The *** A+T-rich region contains non-repetitive sequences,but harbors several features common to the Bombycoidea *** phylogenetic relationships based on Maximum Likelihood method provide a well-supported outline of Saturniidae,which is in accordance with the traditional morphological classification and recent molecular works.

关键词： Atlas moth Attacus atlas Nucleotide composition genomic structure Phylogenetic relationship

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：