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One-step approach for full-thickness skin defect reconstruction in rats using minced split-thickness skin grafts with Pelnac overlay

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Burns & Trauma 2019年第1期7卷 167-179页

作者： Tong Liu Chao Qiu Chi Ben Haihang Li Shihui Zhu Department of Burn Surgery Institute of BurnsThe First Affiliated HospitalNaval Medical UniversityShanghai 200433China Emergency Department The First Affiliated HospitalNaval Medical UniversityShanghai 200433China

Background:Split-thickness skin grafting is the current gold standard for the treatment of traumatic skin ***,for patients with extensive burns,split-thickness skin grafting is limited by donor skin *** split-thickness skin minced into micrografts increases the expansion ratio but may reduce wound repair *** substitutes such as Pelnac can enhance the healing of full-thickness skin wounds,but their application currently requires two *** present study investigated whether it is possible to repair full-thickness skin defects and improve wound healing quality in a single surgery using Pelnac as an overlay of minced split-thickness skin grafts in a rat ***:A full-thickness skin defect model was established using male Sprague-Dawley rats of 10 weeks *** animals were randomly divided into control and experimental groups in which Vaseline gauze and Pelnac,respectively,were overlaid on minced split-thickness skin grafts to repair the *** healing rate and quality were compared between the two *** better illustration of the quality of wound healing,some results were compared with those obtained for normal skin of ***:We found that using Pelnac as an overlay for minced split-thickness skin grafts accelerated wound closure and stimulated cell proliferation and tissue *** addition,this approach enhanced collagen synthesis and increased the formation of basement membrane and dermis as well as the expression of growth factors related to wound healing while reducing scar ***:Using minced split-thickness skin grafts overlaid with Pelnac enables the reconstruction of fullthickness skin defects in a single step and can increase the healing rate while improving the quality of wound healing.

关键词： skin wound healing full-thickness skin defect Minced skin graft Pelnac Split-thickness skin grafts Reconstruction

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Recombinant human epidermal growth factor combined with vacuum sealing drainage for wound healing in Bama pigs

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Military Medical Research 2021年第4期8卷 510-522页

作者： Shuai Wei Wei Wang Li Li Hao-Ye Meng Chun-Zhen Feng Yu-Ying Dong Xi-Chi Fang Qi-Qiang Dong Wen Jiang Hai-Li Xin Zhan-Zhen Li Xin Wang Institute of Orthopaedics Beijing Key Lab of Regenerative Medicine in OrthopaedicsChinese PLA General HospitalBeijing 100583China. Zhoushan Dinghai Guanghua Hospital Zhoushan 316000ZhejiangChina. Tianjin Hospital Tianjin UniversityTianjin 300211China. Geriatric Neurological Department of the Second Medical Center&National Clinical Research Center for Geriatric Diseases Chinese PLA General HospitalBeijing 100853China. Department of Orthopedics Traditional Chinese Medical Hospital of Xinjiang Uygur Autonomous RegionUrumqi 830000China. Department of Stomatology Chinese PLA General HospitalBeijing 100853China. Department of Plastic Surgery General Hospital of Taiyuan Iron and Steel Limited CompanyTaiyuan 030009China. Hand Microsurgery Department Shenzhen People’s HospitalShenzhen 518020GuangdongChina. Third Surgery Department Zhengzhou Renji HospitalZhengzhou 450000China. Department of Orthopedics the First Affiliated Hospital of Medical CollegeShihezi UniversityShiheziXinjiang 832000China. Pharmacy Department Chinese PLA General HospitalBeijing 100853China.

Background:Vacuum sealing drainage(VSD)and epidermal growth factor(EGF)both play an important role in the treatment of *** study aims to explore the effects of the combination of VSD and EGF on wound healing and the optimal concentration and time of ***:We tested the proliferation and migration capacity of HaCaT and L929 cells at different EGF concentrations(0,1,5,10,and 100ng/ml)and different EGF action times(2,10,and 30min).A full-thickness skin defect model was established using male,30-week-old Bama *** experiment included groups as follows:routine dressing change after covering with sterile auxiliary material(Control),continuous negative pressure drainage of the wound(VSD),continuous negative pressure drainage of the wound and injection of EGF 10min followed by removal by continuous lavage(V+E 10min),and continuous negative pressure drainage of the wound and injection of EGF 30min followed by removal by continuous lavage(V+E 30min).The wound healing rate,histological repair effect and collagen deposition were compared among the four ***:An EGF concentration of 10ng/ml and an action time of 10min had optimal effects on the proliferation and migration capacities of HaCaT and L929 *** drug dispersion effect was better than drug infusion after bolus injection effect,and the contact surface was *** with other groups,the V+E 10min group promoted wound healing to the greatest extent and obtained the best histological ***:A recombinant human epidermal growth factor(rhEGF)concentration of 10 ng/ml can promote the proliferation and migration of epithelial cells and fibroblasts to the greatest extent in *** combined with rhEGF kept in place for 10min and then washed,can promote wound healing better than the other treatments in vitro.

关键词： Vacuum sealing drainage Epidermal growth factor skin wound healing full-thickness skin defect

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Co-administration of platelet-rich plasma and small intestinal submucosa is more beneficial than their individual use in promoting acute skin wound healing

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Burns & Trauma 2021年第1期9卷 72-82页

作者： Xiaoxuan Lei Liuhanghang Cheng Yu Yang Mengru Pang Yunqing Dong Xuanru Zhu Caihong Chen Zexin Yao Gang Wu Biao Cheng Tymour Forouzanfar Department of Oral and Maxillofacial Surgery/Pathology Amsterdam UMC and Academic Center for Dentistry Amsterdam(ACTA)Vrije Universiteit AmsterdamAmsterdam Movement ScienceAmsterdam1081HVThe Netherlands Department of Burn and Plastic Surgery General Hospital of Southern Theater CommandGuangzhou510030China Department of Oral Implantology and Prosthetic Dentistry Academic Center for Dentistry Amsterdam(ACTA)University of Amsterdam(UvA)and Vrije Universiteit Amsterdam(VU)Amsterdam1081LAThe Netherlands

Background:Acute skin wounds may compromise the skin barrier,posing a risk of *** intestinal submucosa(SIS)is widely used to treat acute and chronic ***,the efficacy of SIS to accelerate wound healing still needs to be improved to meet clinical *** tackle this problem,platelet-rich plasma(PRP)is used due to its potency to promote proliferation,migration and adhesion of target *** this study,we applied PRP and SIS to skin wounds to explore their effects on wound healing by evaluating re-epithelialization,collagen production,angiogenesis and the inflammatory ***:A1×1-cm full-thickness skin defectwas established in *** mice were divided into four treatment groups:PRP+SIS,PRP,SIS and *** days 3,5,7,10 and 14 post-surgery,tissue specimens were *** and eosin,Masson’s trichrome,immunohistochemical and immunofluorescence double staining were used to visualize epidermal thickness,collagen and vascular regeneration and ***:Wound contraction in the PRP and PRP+SIS groups was significantly greater,compared with the other groups,on days 3 and 5 post-surgery.A histological analysis showed higher collagen expression in the PRP and PRP+SIS groups on day 7,whichwas associated with a thicker epidermal layer on day *** addition,immunohistochemical staining showed that CD31-positive blood vessels and vascular endothelial growth factor expression in the PRP+SIS and PRP groups were significantly higher,compared with the control ***,immunofluorescence double staining showed that the number of M1 and M2 macrophages in the PRP+SIS and PRP groups was higher,compared with the control and SIS groups alone,on day ***,on day 7,the number of M1 macrophages dramatically decreased in the PRP+SIS and PRP *** ratio of M2 to M1 macrophages in the PRP+SIS and PRP groups was 3.97 and 2.93 times that of the control group and 4.56 and 3.37 times that of the SIS group,respectively.

关键词： Small intestinal submucosa Platelet-rich plasma full-thickness skin defect M1macrophages M2macrophages Animal model wound healing skin

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Polydopamine-modified collagen sponge scaffold as a novel dermal regeneration template with sustained release of platelet-rich plasma to accelerate skin repair:A one-step strategy

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Bioactive Materials 2021年第8期6卷 2613-2628页

作者： Zijun Zheng Minxiong Li Pengwei Shi Yanbin Gao Jun Ma Yuchen Li Lei Huang Zhangfeifan Yang Lei Yang Department of Burns Nanfang HospitalSouthern Medical UniversityJingxi StreetBaiyun DistrictGuangzhou510515PR China Department of Emergency Nanfang HospitalSouthern Medical UniversityJingxi StreetBaiyun DistrictGuangzhou510515PR China Department of Statistics University of British Columbia2329 West MallVancouverV6T 1Z4BCCanada

Although employed to release growth factors(GFs)for regenerative medicine,platelet-rich plasma(PRP)has been hindered by issues like burst *** on collagen sponge scaffolds(CSSs)modified with polydopamine(pDA),a novel dermal regeneration template(DRT)was ***,whether it could efficiently deliver PRP and even foster wound healing remained *** this work,after PRP was prepared and pDA-modified CSSs(pDA-CSSs)were fabricated,microscopic observation,GFs release assay and in-vitro biological evaluations of pDA-CSSs with PRP(pDA-CSS@PRP)were performed,followed by BALA-C/nu mice full-thickness skin defects implanted with pDA-CSS@PRP covered by grafted skins(termed as a One-step strategy).As a result,scanning electron microscope demonstrated more immobilized platelets on pDA-CSS′surface with GFs’controlled release via enzyme-linked immunosorbent assay,compared with *** line with enhanced in-vitro proliferation,adhesion and migration of keratinocytes&endothelial cells,pDA-CSS@PRP were histologically revealed to accelerate wound healing with less scar via rapid angiogenesis,arrangement of more mature collagen,guiding cells to spread,*** conclusion,pDA-CSSs have potential to serve as a novel DRT capable of delivering PRP,which may foster full-thickness skin defect healing by means of a One-step strategy.

关键词： Polydopamine Sustained release Platelet-rich plasma Dermal regeneration template full-thickness skin defect

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