One-step approach for full-thickness skin defect reconstruction in rats using minced split-thickness skin grafts with Pelnac overlay

作     者：Tong Liu Chao Qiu Chi Ben Haihang Li Shihui Zhu 

作者机构：Department of Burn SurgeryInstitute of BurnsThe First Affiliated HospitalNaval Medical UniversityShanghai 200433China Emergency DepartmentThe First Affiliated HospitalNaval Medical UniversityShanghai 200433China 

出 版 物：《Burns & Trauma》 (烧伤与创伤（英文）)

年 卷 期：2019年第7卷第1期

页      面：167-179页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

主　　题：Skin wound healing Full-thickness skin defect Minced skin graft Pelnac Split-thickness skin grafts Reconstruction 

摘      要：Background:Split-thickness skin grafting is the current gold standard for the treatment of traumatic skin ***,for patients with extensive burns,split-thickness skin grafting is limited by donor skin *** split-thickness skin minced into micrografts increases the expansion ratio but may reduce wound repair *** substitutes such as Pelnac can enhance the healing of full-thickness skin wounds,but their application currently requires two *** present study investigated whether it is possible to repair full-thickness skin defects and improve wound healing quality in a single surgery using Pelnac as an overlay of minced split-thickness skin grafts in a rat ***:A full-thickness skin defect model was established using male Sprague-Dawley rats of 10 weeks *** animals were randomly divided into control and experimental groups in which Vaseline gauze and Pelnac,respectively,were overlaid on minced split-thickness skin grafts to repair the *** healing rate and quality were compared between the two *** better illustration of the quality of wound healing,some results were compared with those obtained for normal skin of ***:We found that using Pelnac as an overlay for minced split-thickness skin grafts accelerated wound closure and stimulated cell proliferation and tissue *** addition,this approach enhanced collagen synthesis and increased the formation of basement membrane and dermis as well as the expression of growth factors related to wound healing while reducing scar ***:Using minced split-thickness skin grafts overlaid with Pelnac enables the reconstruction of fullthickness skin defects in a single step and can increase the healing rate while improving the quality of wound healing.