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Simultaneous Determination of Seven Alkaloids in fufang zhenzhu tiaozhi capsule by HPLC Coupled with DAD

Chinese Herbal Medicines 2012年第3期4卷 230-236页

作者： Yuan-yuan CHEN Jiao GUO Hui FAN Li-hua HUANG Key Unit of Modulating Liver to Treat Hyperlipemia SATCM Level 3 Laboratory of Lipid Metabolism SATCMGuangdong TCM Key Laboratory for Metabolic DiseasesInstitute of Chinese Medicinal SciencesGuangdong Pharmaceutical UniversityGuangzhou 510006China Guangzhou University of Chinese Medicine Guangzhou 510006China

Objective To establish a reverse-phase liquid chromatography method for the determination of seven alkaloids(magnoflorine,columbamine,jatrorrhizine,epiberberine,coptisine,palmatine,and berberine)in fufang zhenzhu tiaozhi *** Chromatography was performed on a Dionex Acclaim C_(18)column(250 mm×4.6 mm,5.0μm)at 30℃.The mobile phase was composed of acetonitrile-potassium dihydrogen phosphate solution(0.015 mol/L,40:60,including 1.7 g/L sodium dodecyl sulfate and phosphoric acid used to regulate pH value to 3.0),with a flow rate of 1.0 mL/*** detection wavelength was 270 *** The calibration curves of magnoflorine,columbamine,jatrorrhizine,epiberberine,coptisine,palmatine,and berberine were linear in the range of 1.07-10.65,0.78-7.55,0.75-7.50,1.60-15.95,2.69-26.85,2.31-23.10,and 6.04-60.40 mg/*** average recoveries of magnoflorine,columbamine,jatrorrhizine,epiberberine,coptisine,palmatine,and berberine were 101.0%,101.2%,100.1%,100.0%,100.1%,101.1%,and 99.7%,*** The method could be used for the quantitative determination of the preparation.

关键词： alkaloids diode array detection fufang zhenzhu tiaozhi capsule HPLC traditional Chinese medicines

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Analysis of the Constituents in Rat Serum after Oral Administration of fufang zhenzhu tiaozhi capsule by UPLC-Q-TOF-MS/MS

Analysis of the Constituents in Rat Serum after Oral Adminis...

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2013年广东省药师周大会

作者： Xunlong Zhong Jiao Guo LaiyouWang Duosheng Luo Weijian Bei Yuanyuan Chen Kangqi Yan Junhui Peng Key Unit of Modulating Liverto Treat Hyperlipemia SATCM(StateAdministration of Traditional Chinese Medicine) Level 3 laboratory of Lipid Metabolism SATCMInstitute of Chinese Medical Science of Guangdong Pharmaceutical University Guangzhou University of Chinese Medicine

A rapid and sensitive UPLC/Q-TOF-MS method has been established for analysis of the constituents in rat serum after oral administration of fufang zhenzhu tiaozhi(FTZ) capsule,an effective compound prescription for treating hyperlipidemia in *** UPLC/MS information of samples was obtained first in FTZ preparation and FTZ-treated rat *** spectra were acquired in both negative and positive ion modes.36 constituents in rat serum after oral administration of FTZ were detected,including the alkaloids,ginsenosides,pentacyclic triterpenes and their *** chemicals were identified based on the retention time and mass spectrometry data with those of authentic standards or comparison of the literatures reports.27 prototype components originated from FTZ and 9 were the metabolites of the constituents from *** results shed a light on the potential active constituents of the complextraditional Chinese medicinal formula.

关键词： UPLC/Q-TOF-MS Serum Pharmacology Metabolite fufang zhenzhu tiaozhi capsule

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Simultaneous determination of 15 bioactive compounds in fufang zhenzhu tiaozhi capsules by HPLC-DAD-ELSD

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Journal of Chinese Pharmaceutical Sciences 2013年第1期22卷 47-54页

作者：范辉郭姣陈媛媛国家中医药管理局"高脂血症调肝降脂重点研究室" "脂代谢三级实验室"广东省"代谢性疾病中医药防治重点实验室"广东药学院中医药研究院广东广州510006 广东药学院药科学院广东广州510006

fufang zhenzhu tiaozhi capsules （FTZc）, which is consisted of eight traditional Chinese herbal medicines and contains multiple bioactive ingredients, is a patented and clinically approved herbal formulation for the treatment of dyslipidemia. A feasible HPLC-DAD-ELSD method was developed to simultaneously determine 15 bioactive compounds （salidroside, specneuzhenide, magnoflorine, rosmarinic acid, salvianolic acid B, columbamine, jatrorrhizine, epiberberine, coptisine, palmatine, berberine, 5,7-dimethoxycoumarin, ginsenoside Rgl, ginsenoside Rbl and oleanic acid ） in FTZc for its quality control. The multiple wavelength detection mode of DAD was used. The chromatographic separation was performed on an Ultimate XB Cls column with gradient elution. The mobile phase A （acetonitrile） and B （0.25% glacial acetic acid and 0.13% triethylamine in water, v/v） were run at a flow rate of 0.8 mL/min. The developed method showed good precision and accuracy with overall intra- and inter-day variations of 0.7%-1.9% and 0.6%-3.0%, respectively. The recoveries measured at three concentration levels, varied from 95.5% to 103.8%. The validated method was successfully applied for the simultaneous determination of 15 bioactive compounds in three batches of FTZc. The results suggested that the developed method was convenient and reliable, particularly suitable for the routine quality control of FTZc.

关键词： FiPLC-DAD-ELSD fufang zhenzhu tiaozhi capsule Quality control

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