Simultaneous Determination of Seven Alkaloids in Fufang Zhenzhu Tiaozhi Capsule by HPLC Coupled with DAD

作     者：Yuan-yuan CHEN Jiao GUO Hui FAN Li-hua HUANG 

作者机构：Key Unit of Modulating Liver to Treat Hyperlipemia SATCMLevel 3 Laboratory of Lipid Metabolism SATCMGuangdong TCM Key Laboratory for Metabolic DiseasesInstitute of Chinese Medicinal SciencesGuangdong Pharmaceutical UniversityGuangzhou 510006China Guangzhou University of Chinese MedicineGuangzhou 510006China 

出 版 物：《Chinese Herbal Medicines》 (中草药(英文版))

年 卷 期：2012年第4卷第3期

页      面：230-236页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1005[医学-中医学] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Cooperation Project in Industry,Education and Research of Guangdong Province and Ministry of Education of China(2009B090300349) a Cooperation Project of National Science Foundation of Guangdong Province(10351022401000000) 

主　　题：alkaloids diode array detection Fufang Zhenzhu Tiaozhi Capsule HPLC traditional Chinese medicines 

摘      要：Objective To establish a reverse-phase liquid chromatography method for the determination of seven alkaloids(magnoflorine,columbamine,jatrorrhizine,epiberberine,coptisine,palmatine,and berberine)in Fufang Zhenzhu Tiaozhi *** Chromatography was performed on a Dionex Acclaim C_(18)column(250 mm×4.6 mm,5.0μm)at 30℃.The mobile phase was composed of acetonitrile-potassium dihydrogen phosphate solution(0.015 mol/L,40:60,including 1.7 g/L sodium dodecyl sulfate and phosphoric acid used to regulate pH value to 3.0),with a flow rate of 1.0 mL/*** detection wavelength was 270 *** The calibration curves of magnoflorine,columbamine,jatrorrhizine,epiberberine,coptisine,palmatine,and berberine were linear in the range of 1.07-10.65,0.78-7.55,0.75-7.50,1.60-15.95,2.69-26.85,2.31-23.10,and 6.04-60.40 mg/*** average recoveries of magnoflorine,columbamine,jatrorrhizine,epiberberine,coptisine,palmatine,and berberine were 101.0%,101.2%,100.1%,100.0%,100.1%,101.1%,and 99.7%,*** The method could be used for the quantitative determination of the preparation.