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Structural study on a bioactive fructan from the root of Achyranthes bidentata Blume

Chinese Journal of Chemistry 1995年第6期13卷 539-544页

作者： Yu, BA Tian, GY Hui, YZ CHINESE ACAD SCI SHANGHAI INST ORGAN CHEMSTATE KEY LAB BIOORGAN & NAT PROD CHEMSHANGHAI 200032PEOPLES R CHINA

The structure of a saccharide component (Abs), with pronounced activity of improving immunity system, isolated from the root of Achyranthes bidentata Blume, a traditional Chinese herbal medicine, was studied. Based on C-13 NMR, HPLC, and methylation analyses, Abs was shown to be a mixture of short-chain fructans with an average dp of 8, containing more (2-->B) than (2-->1) linked beta-D-fructofuranosyl residues, with branching at O-6 or O-1 of 18% of the D-fructofuranosyl residues.

关键词： structure fructan Abs methylation analysis

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Relationship of Grain fructan Content to Degree of Polymerisation in Different Barleys

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Food and Nutrition Sciences 2014年第6期5卷 581-589页

作者： Csilla Nemeth Annica A.M.Andersson Roger Andersson Elke Mangelsen Chuanxin Sun Per Aman Department of Food Science BioCentreSwedish University of Agricultural Sciences(SLU)UppsalaSweden Department of Plant Biology and Forest Genetics BioCentreSwedish University of Agricultural Sciences(SLU)UppsalaSweden

fructans are important in the survival of plants and also valuable for humans as potentially health promoting food ingredients. In this study fructan content and composition were determined in grains of 20 barley breeding lines and cultivars with a wide variation in chemical composition, morphology and country of origin, grown at one site in Chile. There was significant genotypic variation in grain fructan content ranging from 0.9% to 4.2% of grain dry weight. fructan degree of polymerisation (DP) was analysed using high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). Changes in the distribution of different chain lengths and the pattern of structures of fructan were detected with increasing amount of fructan in the different barleys. A positive correlation was found between fructan content and the relative amount of long chain fructan (DP > 9) (r = 0.54, p = 0.021). Our results provide a basis for selecting promising barley lines and cultivars for further research on fructan in barley breeding with the aim to produce healthy food products.

关键词： fructan Barley Hordeum vulgare L.

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提取小麦果聚糖(fructan)适宜乙醇浓度的选定

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植物生理学报 1986年第3期 51-54页

作者：喻莉明 J.H.Coutts J.A.Schaffer C.J.Nelson 山西省农业科学院小麦研究所美国密苏里大学农学院美国密苏里大学农学院美国密苏里大学农学院临汾哥伦比亚哥伦比亚哥伦比亚

小麦(Trticum aestivum)植株体内主要的贮藏性碳水化合物是果聚糖(fructan)和某些其它糖类。要测定每一组成成分的含量首先必须把单糖、蔗糖与果聚糖分离开来。因此,在测定小麦不同生育期果聚糖及糖类含量的变化时,采用哪一种提取和分... 详细信息

小麦(Trticum aestivum)植株体内主要的贮藏性碳水化合物是果聚糖(fructan)和某些其它糖类。要测定每一组成成分的含量首先必须把单糖、蔗糖与果聚糖分离开来。因此,在测定小麦不同生育期果聚糖及糖类含量的变化时,采用哪一种提取和分离的方

关键词：果聚糖糖类小麦植株碳水化合物乙醇浓度 fructan

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Genome-wide characterization of early response genes to abscisic acid coordinating multiple pathways in Aegilops tauschii

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The Crop Journal 2021年第4期9卷 934-944页

作者： Yu Wei Liangjing Cao Xucheng Huang Xuan Wang Huan Wang Yongchao Song Qiang He Mingjie Lyu Xinwen Hu Jun Liu National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Institute of Tropical Agriculture and Forestry Hainan UniversityHaikou 570228HainanChina Department of Biological Sciences Xi’an Jiaotong-Liverpool UniversitySuzhou 215123JiangsuChina Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China

The diploid wild goat grass Aegilops tauschii(Ae. tauschii, 2 n = 14;DD), as the D-sub genome of common wheat, provides rich germplasm resources for many aspects of wheat breeding. Abscisic acid(ABA) is an essential phytohormone that plays a pivotal role in plant adaptation to abiotic stresses. However,the gene regulation network of Ae. tauschii in response to ABA stress remains unclear. Here, we conducted a time-course strand-specific RNA-sequencing study to globally profile the transcriptome that responded to ABA treatment in Ae. tauschii. We identified 4818 differentially expressed transcription units/genes with time-point-specific induction/repression patterns. Using functional annotation, one-to-one ortholog and comparative transcriptome profiling analyses, we identified 319 ABA-responsive Ae. tauschii orthologs that were also induced/repressed under ABA treatment in hexaploid wheat. On the quantitative trait loci(QTL) used in wheat marker-assisted breeding, we found that the ABA-responsive expression patterns of eight Ae. tauschii orthologs were associated with drought stress tolerance, flowering process and/or grain quality. Of them, the ABA-responsive gene encoding sucrose:sucrose 1-fructosyltransferase in fructan and glucose metabolism pathways showed the most significant association with wheat drought tolerance. The characterization of ABA early-responsive genes in this study provides valuable information for exploring the molecular functions of the regulatory genes and will assist in wheat breeding.

关键词： Wheat Aegilops tauschii Transcriptome Abscisic acid fructan

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Extraction and Powder Product of Fructo-oligosaccharide from Jerusalem Artichoke

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Journal of Food Science and Engineering 2013年第3期3卷 141-148页

作者： Winus Puminat Chowladda Teangpook Division of Food Chemistry and Physic Institution of Food Research and Product Development Kasetsart University P.O. box1043 Kasetsart Bangkok 10900 Thailand Division of Food Processing and Preservation Institution of Food Research and Product Development Kasetsart University P.O.box 1043 Kasetsart Bangkok 10900 Thailand

Fructo-oligosaccharide （FOS） is a type of dietary fiber. It can not be digested by enzymes in the body. It also helps to improve a digestion in the intestine, an excretion system and prevent the cancer. Jerusalem artichoke is scanned and analyzed by High Performance Liquid Chromatography （HPLC） for quantity of FOS. They are selected for extraction and drying process. Studies on the extraction for a powder making, the conditions are optimum for maximum yield. Experiments manage using a factorial real 2 × 4 × 6 in 2 blocks of Randomized Complete Block Design （RCBD） models. The first factor studies on two types of solvent. The second factor is the four levels of temperature in the extraction with water at 25 ℃, 35 ℃, 50℃ and 60℃ for 30 min. The third factor is the ratio of sample per solvent as 5, 6, 10, 15, 20 and 30 times. The data and comparison of average is analyzed by Duncan＇s New Mutiple Range Test at the significant level 0.05. The concentrated extracts are processed to powder by freeze drying, a hot air and vacuum drying. Optimal conditions control the temperature and time by heating in both vacuum and conventional oven. The results of temperature on the extraction are significantly different at a = 0.05. In drying process with high temperature, the color of product is more yellow （b） and less white （L）. The difference of color value is statistically significant at level a = 0.05. The sensory evaluation of food products are added the extracted powder and attribute by panelist. High level acceptance of product is aspectual about texture and flavor. FOS is the trend of functional foods for health. It is focus on low energy and on obesity including increasing the absorption of calcium.

关键词： Fruto-oligosaccharide fructan Jerusalem artichoke HPLC

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