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In vitro antioxidant,antilipoxygenase and antimicrobial activities of extracts from seven climbing plants belonging to the Bignoniaceae

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Journal of Integrative Medicine 2018年第4期16卷 255-262页

作者： Carola Analla Torres Cristina Marisel Perez Zamora Maria Beatriz Nunez Ana Maria Gonzalez Laboratory of Microbiology and Pharmaceutical Technology Department of Basic and Applied SciencesNational University of Chaco AustralPresidencia Roque Saenz Pena 3700ChacoArgentina National Council for Scientific and Technical Research(CONICET) Godoy Cruz 2290Buenos Aires C1425FQBArgentina Institute of Botany of the Northeast(IBONE-CONICET) Sargento Juan Bautista Cabral 2131CorrientesArgentina

Objectives： This study aimed to evaluate the in vitro antioxidant capacity, to determine the anti-inflammatory effect due to lipoxygenase inhibition and to test the antimicrobial activity of ethanolic extracts from leaves of seven climbing species belonging to the Bignoniaceae family. These species are Adenocalyrnrna rnarginatum （Cham.） DC., Amphilophium vauthieri DC., Cuspidaria convoluta （VEIL） A. H. Gentry, Dolichandra dentata （K. Schum.） L. G. Lohmann, Fridericia caudigera （S. Moore） L. G. Lohmann, Fridericia chica （Bonpl.） L. G. Lohmann and Tanaecium selloi （Spreng.） L. G. Lohmann. Methods： The antioxidant activity was evaluated using three methods, 2,2＇-azino-bis（3-ethylbenzothiazo line-6-sulfonic acid）（ABTS）, 2,2-diphenyl-l-picrylhydrazyl （DPPH）, and ferric reducing antioxidant power. Lipoxygenase-inhibiting activity was assayed spectrophotometrically; the result was expressed as percent inhibition. The antimicrobial activity was assessed using the agar disk diffusion method. Minimal inhibitory concentration （MIC） and minimal bactericidal]fungicidal concentration were also determined for each extract against 12 pathogenic bacterial strains of Staphylococcus aureus and seven fungal strains of the Candida genus. The identification of the major compounds present in the most promising extract was established by high-performance liquid chromatography-tandem mass spectrometry. Results： C convoluta, F. caudigera, and F. chica exhibited the best antioxidant activity by scavenging DPPH and ABTS~ radicals and reducing Fe3＋ ion. These extracts showed a notable inhibition of lipnxygenase. F. caudigera was found to have the lower MIC value against S. aureus strains and six Candida species. The extracts of F. caudigera and C. convoluta were active even against methicillin-resistant S. aureus. C convoluta had higher total phenol content, better antioxidant activity and superior anti- inflammatory and antimicrobial activity. The main phenolic compounds found in this extract

关键词： Cuspidaria Fridericia Antioxidants Lipoxygenase Antimicrobials Herbal drugs free radical scavengers

Effects of hydrogen peroxide on in vitro infection of Cryptosporidium parvum

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Progress in Natural Science:Materials International 2001年第12期11卷 68-73页

作者：黄克和,杨世广,MarkC.HEALEY College of Veterinary Medicine Nanjing Agricultural University Nanjing 210095 China Department of Animal Dairy and Veterinary Sciences Utah State University Utah 84322-5600 USA Department of Animal Dairy and Veterinary Sciences Utah State University Utah 84322-5600 USA

The effect of hydrogen peroxide (H2O2) on Cryptosporidium parvum infection and the protective function of free radical scavengers were studied in cultured bovine fallopian tubes epithelial cells. H2O2 treatment at 500 μmol/L and 1 mmol/L significantly inhibited excystation of bleach-treated oocysts (p < 0.01) . At 48 h postinoculation, H2O2 at 500 and 750 μmol/L resulted in a significant (p < 0.01) decrease of C. parvum infection by 35 .77% and 58.16% respectively in the cultured cells, when compared with the untreated control. Surprisingly, the infection was significantly increased from 22.21% to 39.33% (p<0.05) with lower concentration of H202( 50 - 200 μmol/L) . The inhibitory and stimulatory effects of H2O2 treatment on C. parvum infection were, to a certain extent, abolished in the presence of reduced glutathione or mannitol. These observations indicate that reactive oxygen species, such as H2O2, may play an important role in C. parvum infection.

关键词： Cryptosporidium parvum hydrogen peroxide free radical scavengers excystation cell culture.

N-acetylcysteine attenuates alcohol-induced oxidative stress in the rat

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World Journal of Gastroenterology 2003年第1期9卷 125-128页

作者： Resat Ozaras Veysel Tahan Seval Aydin Hafize Uzun Safiye Kaya Hakan Senturk Department of Infectious Diseases and Clinical Microbiology University of IstanbulIstanbulTurkey Department of Internal Medicine University of IstanbulIstanbulTurkey Department of Biochemistry University of IstanbulIstanbulTurkey

AIM: There is increasing evidence that alcohol-induced liverdamage may be associated with increased oxidative *** aimed to investigate free-radical scavenger effect of n-acetylcysteine in rats intragastrically fed with ***: Twenty-four rats divided into three groups werefed with ethanol (6 g/kg/day, Group 1), ethanol and n-acetylcysteine (1 g/kg, Group 2), or isocaloric dextrose(control group, Group 3) for 4 weeks. Then animals weresacrificed under ether anesthesia, intracardiac blood andliver tissues were obtained. Measurements were performedboth in serum and in homogenized liver *** (MDA) level was measured by TBARSmethod. Glutathione peroxidase (GSH-Px) and superoxidedismutase (SOD) levels were studied by commercial ***-Wallis test was used for statistical ***: ALT and AST in Group 1 (154 U/Land 302 U/L,respectively) were higher than those in Group 2 (94 U/L and155 U/L) and Group 3 (99 U/L and 168 U/L) (P＝0.001 forboth). Serum and tissue levels of MDA in Group 1 (1.84 nmol/mL and 96 nmol/100 mg-protein) were higher than Group 2(0.91 nmol/mL and 64 nmol/100 mg-protein) and Group 3(0.94 nmol/mL and 49 nmol/100 mg-protein) (P＜0.001 forboth). On the other hand, serum GSH-Px level in Group 1(8.21 U/g-Hb) was lower than Group 2 (16 U/g-Hb) andGroup 3 (16 U/g-Hb) (P＜0.001). Serum and liver tissue levelsof SOD in Group 1 (11 U/mL and 26 U/100 mg-protein)were lower than Group 2 (18 U/mL and 60 U/100 mg-protein)and Group 3 (20 U/mL and 60 U/100 mg-protein) (P＜0.001for both).CONCLUSION: This study demonstrated that ethanol-induced liver damage is associated with oxidative stress,and co-administration of n-acetylcysteine attenuates thisdamage effectively in rat model.

关键词： Oxidative Stress Acetylcysteine Animals Erythrocytes Ethanol dosage free radical scavengers Glutathione Glutathione Peroxidase Lipid Peroxidation Liver Male Rats Rats, Wistar Research Support, Non-U.S. Gov't Superoxide Dismutase Thiobarbituric Acid Reactive Substances

Antioxidant capacity of extract from Jiangtang Xiaozhi recipe in vitro

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Journal of Traditional Chinese Medicine 2020年第3期40卷 393-400页

作者： Song Wang Yuan Yuan Yu Naixin Gu Haike Zhou Yunfeng Chen Xigong Wang Shuaiyu Fan Kai Ge Zhengyan Jin Long Xu Yang College of Biology and Environmental Sciences Jishou UniversityJishou 416000China Research Center for Pharmacology and Toxicology Institute of Medicinal Plant Development(IMPLAD)Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100193China Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine Ministry of EducationBeijing 100193China IMPLAD Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100193China Beijing Radiation Center Beijing 100875China College of veterinary medicine China Agricultural UniversityBeijing 100083China Key Laboratory of Plant Resources Conservation and Utilization College of Hunan ProvinceJishou UniversityJishou 416000China Experiment Research Center of Xiyuan Hospital China Academy of Chinese Medical SciencesBeijing 100091China

OBJECTIVE: To evaluate the antioxidant capacity of the extract from Jiangtang Xiaozhi recipe(JXR) of in ***: JXR extract was prepared according to previously reported method. In vitro antioxidant assays were used in this experiment, including 1,1-Diphenyl-2-picrylhydrazyl free radical(DPPH) radical scavenging ability, 2-2’-Azinobis-(3-ethylbenzthiazoline-6-sul phonate(ABTS) radical scavenging ability, reducing power assay, fluorescence recovery after photo bleaching assay, β-carotene bleaching assay, ferric thiocyanate assay, and thiobarbituric acid ***: DPPH, ABTS assay showed that JXR extract had distinct effect on scavenging free radicals;reducing power and ferricreducing-antioxidant power assay showed that JXR extract possessed redox ability;β-Carotene bleaching assay and antioxidant activity in a linoleic acid system using ferric thiocyanate method, thiobarbituric acid assay indicated that JXR extract could effectively inhibit lipid peroxidation, and the effect was better than that of Vitamin ***: JXR extract has significant antioxidant capacity in vitro.

关键词： free radical scavengers Lipid peroxidation Antioxidant capacity Jiangtang Xiaozhi recipe

Prevention of grafted liver from reperfusive injury

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World Journal of Gastroenterology 2001年第4期7卷 572-574页

作者： Kai Ma Yang Yu Xian-Min Bu Yan-Jun Li Xian-Wei Dai Liang Wang Yang Dai Hai-Ying Zhao Xiang-Hong Yang Department of General Surgery,Second Clinical College,China Medical University,Shenyang 110003,Liaoning Province,ChinaDepartrnent of Physiology,Shenyang Physical Education College,Shenyang,Liaoning Province,ChinaDepartment of Pathology,China Medical University,Shenyang,Liaoning Province,China Department of General Surgery Second Clinical College China Medical University Shenyang 110003 Liaoning Province China.

INTRODUCTIONThe incidence of primary non-function(PNF)of grafted liver in the early postoperative stage is 2%-23%[1-4],its main cause is the ischemic-rechemic injure[5,6].In this experiment,anisodamine was added into the preserving fluid and the grafted liver was rewarmed at different temperatures to protect the cell membranc and prevent ischemic-reperfusive injury.

关键词： Liver Transplantation Animals Body Temperature Drugs, Chinese Herbal free radical scavengers Hepatocytes Lipid Peroxidation Liver Male Microscopy, Electron Rats Rats, Wistar Reactive Oxygen Species Reperfusion Injury control Research Support, Non-U.S. Gov't Solanaceous Alkaloids Superoxide Dismutase

Phytochemical Analysis of Medicinal Plants with Kidney Protective Activities

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Chinese Journal of Integrative Medicine 2012年第10期18卷 790-800页

作者： Eric Jung-chi Lien Linda Lin-min Lien Rubin Wang Jeffrey Wang Department of Pharmacology and Pharmaceutical Sciences School of PharmacyUniversity of Southern California Department of Chemistry & Biochemistry University of California Department of Pharmaceutical Sciences College of PharmacyWestern University of Health Sciences

In view of the increasing number of patients undergoing kidney dialysis or transplant every year, a survey of the literature on renal protective medicinal plants was undertaken. Most of them are from traditional Chinese medicine （TCM）. Although many of the medicinal herbs reported have not been investigated in terms of active chemical ingredients, some do have compounds well characterized. They fall into a wide range of structures. Several groups of compounds with well established activities are discussed. These include： antioxidant phenolic compounds like tannins, flavonoids, isoflavonoids, unsaturated organic acids and lignans; circulation enhancing compounds like saponins, and basic alkaloids with multiple targets （G-protein coupled receptors）. Also presented are proinflammatory and antiinflammatory fatty acids like linoleic （n-6） and α-Iinolenic （n-3） acids, respectively. Attention is also drawn to the plants containing nephrotoxic aristolochic acid. Different directions of future research are also presented. We hope that this review may provide some leads for new drug discovery and development, and more rational application of TCM.

关键词： kidney protective herbs kidney protection natural products antioxidants free radical scavengers immunomodulating agents antiinflammatory agents structure-activity relationship physicochemical parameters

Antioxidative effect of schisanhenol on human low density lipoprotein and its quantum chemical calculation

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Acta Pharmacologica Sinica 2004年第8期 64-70页

作者： Ling-hong YU, Geng-tao LIU, You-min SUN, Hong-yu ZHANGInstitute of Materia Medico, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100050, China Institute of Theoretical Chemistry, Shandong University, Ji-nan 250100, China Laboratory for Computational Biology, Shandong University of Technology, Zibo 255049, China

AIM: To investigate the effect of schisanhenol (Sal) on copper ion-induced oxidative modulation of human low density lipoprotein (LDL). METHODS: The antioxidative activity of eight schisandrins (DCL) on microsome lipid peroxidation induced by Vit C/NADPH system was first observed, and then, the effect of Sal on Cu2+-induced human LDL oxidation was studied. The generation of malondialdehyde (MDA), lipofuscin, reactive oxygen species (ROS), consumption of a-tocopherol as well as electrophoretic mobility of LDL were determined as criteria of LDL oxidation. Finally, the quantum chemical method was used to calculate the theoretical parameters of eight DCL for elucidating the difference of their antioxidant ability. RESULTS: Sal was shown to be the most active one among eight schizandrins in inhibiting microsome lipid oxidation induced by Vit C/NADPH. Sal 100, 50, and 10μmol/L inhibited production of MDA, lipofuscin and ROS as well as the consumption of a-tocopherol in Cu2+-induced oxidation of human LDL in a

关键词： LDL lipoproteins free radical scavengers molecular structure quantum theory structure-activity relationship xanthenes schisanhenol

Application of Theories of free radicals and Electron Spin Resonance for Research of Traditional Chinese Medicine

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Journal of Traditional Chinese Medicine 2000年第1期20卷 51-58页

作者：段绍瑾 The 2nd Department of Basic Medicine Guang An Men HospitalChina Academy of Traditional Chinese MedicineBejing 100053

The present paper reviews new findings in redoxproperties of the active constituent of Chinese herbalmedicine(CHM),a kind of CHM or a compoundprescription as an ***,we have studiedtheir antioxidant and prooxidant actions with electronspin resonance(ESR).The results show that the activecomponents from over 10 kinds of CHM are able toscavenge the oxygen free radicals but propyl gallate

关键词： Medicine, Chinese Traditional Antioxidants Drugs, Chinese Herbal Electron Spin Resonance Spectroscopy free radical scavengers Humans Neurosecretory Systems Oxidative Stress