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Heterogeneous expression of Osa-MIR156bc increases abiotic stress resistance and forage quality of alfalfa

The Crop Journal 2021年第5期9卷 1135-1144页

作者： Kexin Wang Yanrong Liu Fengkui Teng Huifang Cen Jianpin Yan Shiwen Lin Dayong Li Wanjun Zhang College of Grassland Science and Technology China Agricultural UniversityBeijing 100193China Key Lab of Grassland Science in Beijing China Agricultural UniversityBeijing 100193China College of Biological Science China Agricultural UniversityBeijing 100193China College of Life Sciences Shandong Normal UniversityJinan 250014ShandongChina

Alfalfa (Medicago sativa L.) is the most widely cultivated perennial leguminous forage crop *** RNA156 (miR156) precursor genes from dicotyledonous species are reportedly useful for improving alfalfa plant architecture and abiotic stress ***,there has been no report on whether a miR156 precursor gene from a monocotyledonous species functions in *** introduced two tandem precursor genes of miR156,rice Osa-MIR156b and Osa-MIR156c (Osa-MIR156bc),into *** expression of miR156 in the transgenic (TG) alfalfa was significantly *** to wild-type plants,the TG plants overexpressing miR156 had more branches and leaves and showed improved salt and drought *** of miR156 slightly reduced plant height,but the biomass yield of TG plants grown in flowerpots was still *** quality of TG plants was markedly improved by reduction of acid detergent lignin (ADL) content and increase in crude protein *** expression of the putative miR156 target genes Ms SPL6,Ms SPL12,and Ms SPL13 in TG plants was repressed by miR156overexpression,and that of all tested Ms SPLs would be sharply increased under drought or salt *** sequencing revealed that overexpression of miR156 affected the expression of genes associated with abiotic stress resistance and plant development in multiple *** first report of overexpression of monocot miR156 precursors in alfalfa sheds light on the function of mi RNA156 precursors from the monocot species rice that could be used for genetic improvement of the dicot forage crop alfalfa.

关键词： Alfalfa miR156 Biomass yield forage quality Abiotic stress

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Influence of preceding crop and tillage system on forage yield and quality of selected summer grass and legume forage crops under arid conditions

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Journal of Integrative Agriculture 2022年第11期21卷 3329-3344页

作者： Hend H.M.HASSAN El-Sayed E.A.EL-SOBKY Elsayed MANSOUR Ahmed S.M.El-KHOLY Mohamed F.AWAD Hayat ULLAH Avishek DATTA forage Crops Research Department Field Crops Research InstituteAgricultural Research CenterGiza 12619Egypt Agronomy Department Faculty of AgricultureZagazig UniversityZagazig 44519Egypt Department of Biology College of ScienceTaif UniversityTaif 21944Saudi Arabia Agricultural Systems and Engineering Department of FoodAgriculture and BioresourcesSchool of EnvironmentResources and DevelopmentAsian Institute of TechnologyKlong LuangPathum Thani 12120Thailand

Among the crop production factors,preceding crop and tillage management affect the sustainable use of soil resources and ultimately crop growth and *** study aimed at investigating the impact of preceding winter crops(grass or legume)and different tillage systems on forage yield,quality and nutritive values of three summer grass(Sudan grass,pearl millet and teosinte)and two legume forage crops(cowpea and guar)under arid *** results exhibited that growing forage crops after legumes(as berseem clover)produced the highest fresh and dry forage yields and quality attributes compared with grasses(as wheat)with the exception of crude fiber content,which was ***,tillage practices showed positive impact on forage yields and quality *** maximum forage yields and quality parameters were recorded under conventional tillage(CT)practice compared with reduced tillage(RT)and no-tillage(NT)*** the evaluated crops,the highest yields of fresh forage,dry forage,crude fiber,crude protein and total digestible nutrient were exhibited by grass forage crops(Sudan grass,pearl millet and teosinte),whereas the highest crude protein content and the digestible energy values were produced by legume forage crops(cowpea and guar).The maximum fresh forage,dry forage,crude fiber,crude protein,total digestible nutrient and digestible crude protein yields were produced by pearl millet followed by Sudan grass under CT and RT after berseem *** highest net return was recorded by sowing pearl millet after berseem clover and applying CT followed by RT practices,which could be recommended for the commercial ***,it could be assumed that the combination of growing grass forage crops after legume crops under CT or RT systems could enhance forage crop yield and quality with an improvement in soil properties for sustainable agriculture with low cost and the highest net income.

关键词： soil tillage preceding crop forage quality nutritive values net return

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Milk fatty acids profiles and milk production from dairy cows fed different forage quality diets

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Animal Nutrition 2016年第4期2卷 329-333页

作者： Shuaiwang Liu Runhou Zhang Rong Kang Jinzhu Meng Changjin Ao College of Animal Science Inner Mongolia Agricultural University Wujiang River Institute of Agriculture & Forestry Economics Tongren University

Thirty lactating Holstein cows were used to investigate the effects of different forages quality on milk fatty acids(FA) profiles and production. The cows were assigned to 3 dietary treatments(n = 10 per treatment) in a randomized block design with 3 repeated measures. They were fed the experimental diets for 90 d with 3 days of collection of samples for analysis at about 27 d intervals(samples were collected on days 28,29,30, 58, 59, 60.88, 89 and 90). The treatments were(DM basis): 1) mixed forages diet(MF) consisting of 3.7% Chinese wild rye, 26.7% corn silage and 23.4% alfalfa hay; 2) corn stalk diet 1(CS1) where corn stalk was used to formulate a similar chemical nutrient level to MF; 3) corn stalk diet 2(CS2) which used corn stalk to formulate a similar forage level to MF for the diet. Dry matter intake and BW were similar between treatments, but daily milk yield, milk fat and protein yield decreased(P forages diets is not an effective way to increase milk fat synthesis and milk production.

关键词： Dairy cow forage quality Milk fatty acids profiles Milk production

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forage quality of five important grasses at Sar-Aliabad summer rangelands in Golestan province,Iran

Forage quality of five important grasses at Sar-Aliabad summ...

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ⅩⅪ International Grassland Congress and the Ⅷ International Rangeland Congress

作者： S.A.Hosseini M.Mesdaghi Researcher Golestan agricultural and natural resources research centerP.O.Box 49157-93354 GorganIran Professor of Rangeland department of Golestan University GorganIran

＜正＞Introduction An initial knowledge of forage quality is essential for determining rangeland grazing capacity,and for sustainable range *** an investigation of variation in nutritive quality of forage in different... 详细信息

关键词： forage quality crude protein acid detergent fiber dry matter digestibility metabolizable energy

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Kudzu forage quality Evaluation as an Animal Feed Source

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American Journal of Plant Sciences 2016年第4期7卷 702-707页

作者： Dylan Glass Safaa Al-Hamdani Biology Department Jacksonville State University Jacksonville USA

The nutritional value of kudzu (Pueraria lobata) was evaluated as a potential animal feed. Randomly selected samples from natural habitats with Jacksonville area (Alabama, USA) were utilized in the study. Kudzu leaves showed significantly higher dry matter and crude protein content than the stems;whereas, ADF fiber was significantly lower in the leaves. However, NDF and total digestible nutrients (TDN) were similar in the leaves and the stems. The leaf part of kudzu was especially rich in CP (24.46%) and met the requirements and recommendations for most ruminants. The TDN value for kudzu stem is 55.99%, which falls below but within the recommended range. Macronutrients concentration between the stem and leaf of kudzu, nitrogen, calcium, and magnesium showed a significantly higher in the leaf than the stem. However, phosphorous was similar in concentration in the stem and the leaf, and potassium was higher in the stem. In regard to the micronutrients, the only significant difference between the leaf and the stem was iron. However, iron, manganese, zinc, and copper were all above the recommended concentrations by the National Research Council. In general, kudzu can be considered a good source for animal feed, especially the leaf, which shows a higher value in most of the determined criteria in comparison to what was recommended by the National Research Council. This should provide a new outlook to kudzu, which has been considered a noxious weed and help in the utilization of kudzu in a positive way in the southern region of the US.

关键词： Kudzu (Pueraria lobata) Macronutrients Micronutrients Nutritive Value forage quality

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Leaf growth dynamics and forage quality in temperate and tropical grasses

Leaf growth dynamics and forage quality in temperate and tro...

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ⅩⅪ International Grassland Congress and the Ⅷ International Rangeland Congress

作者： Agnusdei M.G. Nennin F. Di Marco O.N. Aello M.S. Instituto Nacionai de Tecnologia Agropecuaria(INTA) Fac.Ciencias.Agrarias Universidad National de Mar del Plata.CC 276.7620 Balcarce.Argentina

＜正＞Introduction In grasses,maximum leaf-blade size is achieved at ligulae ***,cell wall（CW） accumulation,as well as the occurrence of changes in CW chemical and structural features continues during leaf *** ontogenic changes in forage quality are currently described by the increase of indigestible NDF（INDF）.At present,many studies are focused on the INDF kinetics during leaf development with modeling *** aim of this study was to evaluate the in vitro kinetic of INDF accumulation in individual leaves along their development in three species differing in their potential leaf length and photosynthetic path-way.

关键词： C3/C4 species forage quality indigestible NDF leaf aging

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Effects of Meteorological Factors on Overwintering Ability,Yield and quality of forage Rape

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Agricultural Biotechnology 2021年第3期10卷 15-21,25页

作者： Yao ZHANG Junzhu GE Guangsheng ZHOU Xidong WU Yong an YANG Haipeng HOU Qian LIANG Zhiqi MA College of Agronomy&Resources and Environment Tianjin Agricultural UniversityTianjin 300392China National Key Lab of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China Tianjin High quality Agricultural Products Development Demonstration Center Tianjin 300061China Tianjin Planting Industry Development Service Center Tianjin 300061China

In order to investigate the effects of meteorological factors on rape overwintering ability,forage yield and quality of rape in the North China plain,Brassia campestris *** Brassica napus *** used in this *** results showed that compared with the *** ***,the growth period of *** *** shortened by 10-15 d,the overwintering rate(WR)increased by 50.6%,and the density after winter(PD)increased by 41.5%.The fresh forage yield(FFY)and dry forage yield(DFY)of the *** *** significantly increased by 40.9%and 38.1%compared with the *** ***.,respectively,while the forage quality of the *** *** rape was significantly better than that of the *** *** with the *** ***,the crude protein(CP),fat,ash and total fatty acid(TFA)contents of the *** *** of rape increased by 27.6%,42.9%,23.9%and 52.3%,respectively,and the milk productivity(HM),relative forage value(RFV)and relative forage quality(RFQ)increased by 14.0%,16.2%and 42.1%,*** light and heat resources before wintering increased the WR and PD(P0.05),and lower temperature during the wintering period led to lower WR(P0.05).The contents of CP,fat and TFA of rape had an extremely significant negative correlation with the temperature and sunshine hours before wintering,but an extremely significant positive correlation with the temperature during the wintering period and after regreening,as well as the sunshine hours and rainfall during the wintering period;and HM had an extremely significant positive correlation with the temperature,sunshine hours and rainfall during the wintering period,while RFV and RFQ were only extremely significantly positively correlated with the maximum temperature and *** summary,in the North China Plain

关键词： forage rape Meteorological factors Wintering ability forage yield forage quality

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Yield and quality of‘Guangyi’ Whipgrass forage at Varying Fertility Levels

Yield and Quality of‘Guangyi’ Whipgrass Forage at Varying ...

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中国—澳大利亚可持续性草地管理研讨会

作者： Liu Xiaobo Yang Chunhua~* Chen Lingzhi He Lingfei Shu Simin Department of Grassland Science Animal Science and Technological College Sichuan Agricultural Universit Ya’an 625014 Sichuan Province PRC.

＜正＞Introduction:‘Guangyi’ whipgrass（Hemarthria compressa cv. Guangyi） is extensive used in subtropical and tropical regions of China as a pasture and fodder *** to now,little experimental data are available on the relationship between soil fertility levels and forage quality of *** objectives of this study were to determine the effect of various levels of N,P,K on the yield and quality of whipgrass during production period,and evaluate the quality in relation to the Nutrient Requirements of Dairy Cattle（2001） established by the National Research Council（NRC）.

关键词： Whipgrass fertility levels dry matter yield forage quality in vitro dry matter digestibility

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Identifying priority protection areas of key food resources of the giant panda

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动物学研究(英文) 2023年第5期44卷 860-866页

作者： Ying-Jie Ma Meng Wang Xiao-Yu Hu Xiao-Dong Gu Yu-Mei Li Fu-Wen Wei Yong-Gang Nie Key Laboratory of Animal Ecology and Conservation Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Xinjiang Key Laboratory of Biological Resources and Genetic Engineering College of Life Science and TechnologyXinjiang UniversityUrumqiXinjiang 830017China Sichuan Giant Panda National Park Administration ChengduSichuan 610081China Center for Evolution and Conservation Biology Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou)GuangzhouGuangdong 511458China

Animals that live in seasonal environments adjust their reproduction cycle to optimize seasonal forage *** pandas(Ailuropoda melanoleuca)are seasonal altitudinal migrants that feed on bamboo shoots and leaves with different nutritional ***,the importance of bamboo shoots to giant pandas,especially small and isolated populations,is not fully ***,we explored whether mating time of giant pandas is shaped by bamboo shoot *** also assessed the intensity of ongoing bamboo shoot harvesting by local communities in 42 giant panda reserves based on questionnaire *** intensity and protection levels of bamboo shoot harvesting were *** these data,we developed a priority ranking scheme of protection areas for this key food *** study showed that pandas time their mating behavior to coincide with bamboo shoot phenology due to the high nutritional demands associated with mating and *** also found that bamboo shoots were not well protected in many ***,Daxiangling,and Xiaoxiangling,containing the most isolated panda populations,were identified as the areas with the most urgent need of ***,equal attention should be paid to the Qiong L-B population,as this region holds considerable potential to serve as a corridor between the Minshan and Qionglai *** address the challenges posed by bamboo shoot harvesting,we recommend establishing more practical bamboo shoot management policies,increasing public awareness of bamboo shoot protection,and providing alternative sources of income for local communities.

关键词： Giant panda forage quality Bamboo shoot harvesting Reproduction timing Conservation management

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QTL analysis of the developmental changes in cell wall components and forage digestibility in maize(Zea mays L.)

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Journal of Integrative Agriculture 2022年第12期21卷 3501-3513页

作者： LI Kun YANG Xue LIU Xiao-gang HU Xiao-jiao WU Yu-jin WANG Qi MA Fei-qian LI Shu-qiang WANG Hong-wu LIU Zhi-fang HUANG Chang-ling Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081P.R.China National Engineering Laboratory for Crop Molecular Breeding Beijing 10081P.R.China

Cell wall architecture plays a key role in stalk strength and forage ***,cellulose,and hemicellulose are the three main components of plant cell walls,and they can impact stalk quality by affecting the structure and strength of the cell *** explore cell wall development during secondary cell wall lignification in maize stalks,conventional and conditional genetic mapping were used to identify the dynamic quantitative trait loci(QTLs)of the cell wall components and digestibility traits during five growth stages after *** detergent lignin(ADL),cellulose(CEL),acid detergent fiber(ADF),neutral detergent fiber(NDF),and in vitro dry matter digestibility(IVDMD)were evaluated in a maize recombinant inbred line(RIL)***,CEL,ADF,and NDF gradually increased from 10 to 40 days after silking(DAS),and then they *** initially decreased until 40 DAS,and then it increased *** QTLs were identified for the five traits,and each accounted for 3.48–24.04%of the phenotypic *** QTL hotspots were found,and they were localized in the 1.08,2.04,2.07,7.03,8.05,and 9.03 bins of the maize *** the interval of the pleiotropic QTL identified in bin 1.08 of the maize genome,six genes associated with cell wall component biosynthesis were identified as potential candidate genes for stalk strength as well as cell wall-related *** addition,26 conditional QTLs were detected in the five stages for all of the investigated ***-two of the 26 conditional QTLs were found at 30 DAS conditioned using the values of 20 DAS,and at 50 DAS conditioned using the values of 40 *** results indicated that cell wall-related traits are regulated by many genes,which are specifically expressed at different stages after *** improvements in both forage digestibility and lodging resistance could be achieved by pyramiding multiple beneficial QTL alleles identified in this study.

关键词： quantitative trait loci maize(Zea mays L.) cell wall components forage quality

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