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Detection of Staphylococcus Aureus using quantum dots as fluorescence labels

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International Journal of Agricultural and Biological Engineering 2014年第1期7卷 77-83页

作者： Hu Yaohua Wang Chengcheng Bai Bing Li Mintong Ronghui Wang Yanbin Li College of Mechanical and Electronic Engineering Northwest A&F UniversityYanglingShaanxi 712100China Department of Biological and Agricultural Engineering University of ArkansasFayettevilleAR 72701USA

Staphylococcus aureus(***)has been identified as one of the major foodborne pathogenic *** development of rapid detection methods for *** is needed for assuring food *** this study,quantum dots were used as fluorescent labels in an immunoassay for quantitative detection of ***,biotin-labeled *** antibody was conjugated with streptavidin-coated magnetic nanobeads(180 nm diameter)and used to separate *** *** streptavidin coated quantum dots(QDs)were conjugated with biotin-labeled *** antibody and used as the fluorescence labels to mix with the separated *** the fluorescence intensity of the bead-cell-QD complexes was measured at a wavelength of 620 nm.A linear relationship between *** cell number(X)and fluorescence intensity(Y)was found for cell numbers ranging from 10^(3) to 10^(6) CFU(Colony Forming Unit)/mL,and the detection limit was 10^(3) CFU/*** regression model can be expressed as Y=7.68X+35.06 with R^(2)=*** detection of *** in food sample was explored *** fluorescence intensity of food sample was close to the background,so it was not *** study will focus on the application of the method for detection of *** in food sample.

关键词： staphylococcus aureus fluorescence measurement biological detection quantum dots microbial monitoring food safety

Penicillium and patulin distribution in pears contaminated with Penicillium expansum. Determination of patulin in pears by UHPLC-MS/MS

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Journal of Integrative Agriculture 2017年第7期16卷 1645-1651页

作者： WEI Dong-mei XU Jun DONG Feng-shou LIU Xin-gang WU Xiao-hu ZHENG Yong-quan Risk Assessment Laboratory for Biological Hazards of Agricultural Product Quality and safety Ministry of Agriculture/Institute of Plant Protection Chinese Academy of Agricultural Sciences College of Plant Protection Shenyang Agricultural University

The danger of mycotoxin contamination entering the food supply through post-harvest infection is of perennial concern to food safety experts. To explore the distribution of Penicillium expansum and diffusion of its mycotoxin, patulin, in blue mold-damaged pears, Pyrus bretschneideri Rehd. cv. Yali obtained from markets and orchards in China were artificially inoculated with P. expansum and assayed for patulin accumulation and degree of fungal colonization. The inoculated pears were incubated until the lesions were 5, 10, 20, or 30 mm in diameter. We sampled tissue at a range of distances from the lesion, measured the spread of Penicillium by plate colony-counting methods, and used UHPLC-MS/MS to detect and quantify the patulin concentration. More P. expansum colony-forming units were isolated from pears with a higher degree of decay. Farther from the lesion, the fewer P. expansum colonies were observed, and the lower the patulin content detected. We found a significant difference in the patulin content between samples due to lesion size, and also in tissue sampled 10 mm away from the lesion. In consideration of this finding, to ensure food safety, we recommend that when a blue mold rot lesion on pear is 5, 10, or 20 mm in diameter, 20, 30, and 40 mm beyond the lesion should be removed, respectively. If a lesion surpasses 30 mm in diameter, the whole pear should be thrown away.

关键词： pear Penicillium expansum patulin food safety UHPLC-MS/MS

Cadmium accumulation in different pakchoi cultivars and screening for pollution-safe cultivars

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第6期13卷 494-502页

作者： Ying CHEN Ting-qiang LI Xuan HAN Zhe-li DING Xiao-e YANG Ye-fei JIN Ministry of Education Key Laboratory of Environmental Remediation and Ecological Health College of Environmental and Resource SciencesZhejiang UniversityHangzhou 310058China College of Life Sciences Shaoxing College o fArts and SciencesShaoxing 312000China

The selection and breeding of pollution-safe cultivars (PSCs) is a practicable and cost-effective approach to minimize the influx of heavy metal to the human food *** this study,both pot-culture and field experiments were conducted to identify and screen out cadmium pollution-safe cultivars (Cd-PSCs) from 50 pakchoi (Brassica rapa ***) cultivars for food *** treated with 1.0 or 2.5 mg/kg Cd,most of the pakchoi cultivars (>70%) showed greater or similar shoot biomass when compared with the *** result indicates that pakchoi has a considerable tolerance to soil Cd *** concentrations in the shoot varied significantly (Pfood safety.

关键词： Cadmium pollution food safety Pakchoi Pollution-safe cultivars

Marination ingredients on meat quality and safety—a review

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food Quality and safety 2023年第3期7卷 408-424页

作者： Syed Md.Ehsanur Rahman Sharmeen Islam 潘俊宇孔德薇奚倩都启晶杨永新王军 Deog-Hwan Oh 韩荣伟 College of food Science and Engineering Shandong Technology Innovation Center of Special FoodQingdao Agricultural UniversityQingdaoChina Department of Animal Science Bangladesh Agricultural UniversityMymensinghBangladesh College of food Science and Engineering Tarim UniversityAlarChina Qingdao Special food Research Institute QingdaoChina Department of food Science and Biotechnology College of Agriculture and Life ScienceKangwon National UniversityChuncheonGangwonRepublic of Korea

The practice of utilizing various categories of ingredients for marination has been prevalent in both home cooking and the meat industry for an extended *** and meat products treated with various marination ingredients either alone or in combination with multiple marination processes can enhance color,flavor,and tenderness,while also improving their shelf life by reducing the growth of pathogenic microorganisms and lipid *** narrative review aims to examine all recent scientific literature on various meat and meat products subjected to *** Google Scholar,PubMed,and Web of Science,the review summarizes all recent marinated and marination research articles,including types of marination ingredients,marination method,marinade mechanism,effect on sensory and nutritional quality,safety,shelf life,and health implications,resulting in a comprehensive overview of all information under marinades and marination for all scientists and food sectors *** highlighted information will indicate future directions for the development of marination ingredients in the meat industry.

关键词： Marinade marination food safety meat and meat products

Bacteriophages as antimicrobial agents against major pathogens in swine： a review

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Journal of Animal Science and Biotechnology 2016年第1期7卷 50-56页

作者： Jiancheng Zhang Zhen Li Zhenhui Cao Lili Wang Xiaoyu Li Shuying Li Yongping Xu School of Life Science and Biotechnology Dalian University of TechnologyDalian 116024China Ministry of Education Center for food safety of Animal Origin Dalian 116600China Faculty of Animal Science and Technology Yunnan Agricultural UniversityKunming 650201China Dalian SEM Bio-Engineering Technology Co.Ltd. Dalian 116620China

In recent years, the development of antibiotic resistant bacteria has become a global concern which has prompted research into the development of alternative disease control strategies for the swine industry. Bacteriophages （viruses that infect bacteria） offer the prospect of a sustainable alternative approach against bacterial pathogens with the flexibility of being applied therapeutically or for biological control purposes. This paper reviews the use of phages as an antimicrobial strategy for controlling critical pathogens including Salmonella and Eschefich[a coli with an emphasis on the application of phages for improving performance and nutrient digestibility in swine operations as well as in controlling zoonotic human diseases by reducing the bacterial load spread from pork products to humans through the meat,

关键词： Antibiotic resistance Bacteriophage food safety Phage therapy Swine

Research progress on antibiotic resistance of Salmonella

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food Quality and safety 2022年第3期6卷 355-364页

作者： Yizhe Wang Hengwei Ge Xinyue Wei Xihong Zhao Research Center for Environmental Ecology and Engineering Key Laboratory for Green Chemical Process of Ministry of EducationHubei Key Laboratory of Novel Reactor and Green Chemical TechnologySchool of Environmental Ecology and Biological EngineeringWuhan Institut of TechnologyWuhanChina

Antibiotic abuse results in various antibiotic resistance among a number of foodborne bacteria,posing a severe threat to food *** resistance genes are commonly detected in foodborne pathogens,which has sparked much interest in finding solutions to these *** strategies against these drug-resistant pathogens have been studied,including new antibiotics and ***,a powerful tool has been introduced in the fight against drug-resistant pathogens,namely,clustered regularly interspaced short palindromic repeats-CRiSPR associated(CRISPR-Cas)system aggregated by a prokaryotic defense *** review summarizes the mechanism of antibiotic resistance in Salmonella and resistance to common antibiotics,analyzes the relationship between Salmonella CRiSPR-Cas and antibiotic resistance,discusses the changes in antibiotic resistance on the structure and function of CRiSPR-Cas,andfinally predicts the mechanism of CRISPR-Cas intervention in Salmonella antibiotic *** the future,CRiSPR-Cas is expected to become an important tool to reduce the threat of antibiotic-resistant pathogens in food safety.

关键词： foodborne pathogens antibiotic resistance CRISPR-Cas food safety

Contamination of microgreens by Salmonella enterica and Escherichia coli is influenced by selection breeding in chicory(Cichorium intybus L.)

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food Quality and safety 2022年第2期6卷 244-251页

作者： Anna Lenzi Ada Baldi Letizia Lombardelli Stefania Truschi Massimiliano Marvasi Piero Bruschi Department of Agriculture FoodEnvironment and Forestry(DAGRI)University of FlorenceFlorenceItaly Department of Biology University of FlorenceFlorenceItaly

The aim of this study was to assess whether selection breeding in chicory(Cichorium intybus L.)led changes in the susceptibility to Salmonella enterica and Escherichia coli contamination and whether the anatomical traits of the leaves are involved in the possible *** chicory genotypes subjected to different intensities of selection were compared at the microgreen *** retention was evaluated after leaf incubation for 1.5 h on the surface of the bacterial suspension,followed by rinsing,grinding,plating on selective media,and colony forming unit(CFU)*** density of stomata and trichomes,total stomatal length and width,stomatal pit width,surface roughness and sharpness were *** intensively selected genotype(Witloof)was significantly more prone to contamination(2.9±0.3 lg CFU/cm^(2))as the average of the two bacterial types than the wild accession(Wild;2.3±0.4 lg CFU/cm^(2))and the moderately selected genotypes(two leaf chicories,Catalogna type,and root chicory'Magdeburg';on average,1.9±0.3 lg CFU/cm^(2)).Witloof microgreens also showed larger stomata(on average+34%for stoma width and+44%for pit width),which could justify,at least in part,the higher susceptibility to enterobacterial *** fact,when contamination was performed in the dark(closed stomata),the bacterial retention in Witloof was significantly reduced in comparison with the opened stomata(-44%)and in Wild(-26%).Differences in retention between Witloof and Wild were still observed after UV *** hierarchical clustering performed by grouping the leaf anatomical features was consistent with the chicory genetic *** results suggest that the domestication process can affect the safety of produce and that the micromorphological traits of the leaves may be involved.

关键词： chicory genotypes enterobacteria contamination raw-consumed vegetables micro vegetables selective breeding food safety stoma

Biological control of aflatoxin contamination of crops

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第10期9卷 787-792页

作者： Yan-ni YIN Lei-yan YAN Jin-hua JIANG Zhong-hua MA Institute of Biotechnology Zhejiang University Hangzhou 310029 China

Aflatoxins produced primarily by two closely related fungi, Aspergillus flavus and Aspergillus parasiticus, are mutagenic and carcinogenic in animals and humans. Of many approaches investigated to manage aflatoxin contamination, bio-logical control method has shown great promise. Numerous organisms, including bacteria, yeasts and nontoxigenic fungal strains of A. flavus and A. parasiticus, have been tested for their ability in controlling aflatoxin contamination. Great successes in reducing aflatoxin contamination have been achieved by application of nontoxigenic strains of A. flavus and A. parasiticus in fields of cotton, peanut, maize and pistachio. The nontoxigenic strains applied to soil occupy the same niches as the natural occurring toxigenic strains. They, therefore, are capable of competing and displacing toxigenic strains. In this paper, we review recent development in biological control of aflatoxin contamination.

关键词： Aflatoxin Aspergillus Biocontrol food safety

Soil Contamination due to E-Waste Disposal and Recycling Activities: A Review with Special Focus on China

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Pedosphere 2012年第4期22卷 470-488页

作者： G. ECHEVARRIA T. STERCKEMAN M. O. SIMONNOT J. L. MOREL INPL(ENSAIA)/INRA Laboratoire Sols et Environnement 2 Avenue de la For et de Haye BP 172 54505 Vandoeuvre-lès-Nancy Cedex (France) Laboratoire Réactions et Génie des Procédés (LRGP) Université de Lorraine-CNRS (UPR 3349) 1 Rue Grandville BP20451 54001 Nancy Cedex (France)

Considering that even contaminated soils are a potential resource for agricultural production, it is essential to develop a set of cropping systems to allow a safe and sustainable agriculture on contaminated lands while avoiding any transfer of toxic trace elements to the food chain. In this review, three main strategies, i.e., phytoexclusion, phytostabilization, and phytoextraction, are proposed to establish cropping systems for production of edible and non-edible plants, and for extraction of elements for industrial use. For safe production of food crops, the selection of low-accumulating plants/cultivars and the application of soil amendments are of vital importance. Phytostabilization using non-food energy and fiber plants can provide additional renewable energy sources and economic benefit with minimum cost of agricultural measures. Phytoextracting trace elements （e.g., As, Cd, Ni, and Zn） using hyperaccumulator species is more suitable for slightly and moderately polluted sites, and phytomining of Ni from serpentine soils has shown a great potential to extract Ni-containing bio-ores of economic interests. We conclude that appropriate combinations of soil types, plant species/cultivars, and agronomic practices can restrict trace metal transfer to the food chain and/or extract energy and metals of industrial use and allow safe agricultural activities.

关键词： agronomic practices food safety hyperaccumulator phytoremediation trace element