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Endoscopic ultrasound guided fine needle aspiration and useful ancillary methods

World Journal of Gastroenterology 2014年第39期20卷 14292-14300页

作者： Mario Tadic Tajana Stoos-Veic Rajko Kusec Department of Gastroenterology Dubrava University Hospital Zagreb University of Zagreb Faculty of Pharmacy and Biochemistry 10040 Zagreb Croatia Department of Pathology and cytology Dubrava University Hospital Zagreb 10040 Zagreb Croatia Division of molecular diagnostics and genetics Dubrava University Hospital ZagrebUniversity of ZagrebSchool of Medicine 10040 Zagreb Croatia

The role of endoscopic ultrasound(EUS) in evaluating pancreatic pathology has been well documented from the beginning of its clinical use. High spatial resolution and the close proximity to the evaluated organs within the mediastinum and abdominal cavity allow detection of small focal lesions and precise tissue acquisition from suspected lesions within the reach of this method. fine needle aspiration(FNA) is considered of additional value to EUS and is performed to obtain tissue diagnosis. Tissue acquisition from suspected lesions for cytological or histological analysis allows, not only the differentiation between malignant and non-malignant lesions, but, in most cases, also the accurate distinction between the various types of malignant lesions. It is well documented that the best results are achieved only if an adequate sample is obtained for further analysis, if the material is processed in an appropriate way, and if adequate ancillary methods are performed. This is a multi-step process and could be quite a challenge in some cases. In this article, we discuss the technical aspects of tissue acquisition by EUS-guided-FNA(EUS-FNA), as well as the role of an on-site cytopathologist, various means of specimen processing, and the selection of the appropriate ancillary method for providing an accurate tissue diagnosis and maximizing the yield of this method. The main goal of this review is to alert endosonographers, not only to the different possibilities of tissue acquisition, namely EUS-FNA, but also to bring to their attention the importance of proper sample processing in the evaluation of various lesions in the gastrointestinal tract and other accessible organs. All aspects of tissue acquisition(needles, suction, use of stylet, complications, etc.) have been well discussed lately. Adequate tissue samples enable comprehensive diagnoses, which answer the main clinical questions, thus enabling targeted therapy.

关键词： Endoscopic ultrasound Endoscopic ultrasound-guided fine needle aspiration Endoscopic ultrasound-guided tissue acquisition fine needle aspiration cytology Ancillary studies Molecular testing Flow cytometry immunophenotyping

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Progressively Enlarging Goiter:Case Reports of Primary Thyroid Lymphoma and Literature Review

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Current Medical Science 2020年第3期40卷 518-522页

作者： Xia-ming LIU De-lin MA Gang YUAN Jun-hui XIE Department of Urology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Department of Endocrinology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China

Primary thyroid lymphoma(PTL)is an exceptionally rare and highly aggressive potentially curable malignant *** report three typical cases of PTL referred to our *** three cases had long history of Hashimoto’s thyroiditis,and presented with progressively enlarging neck *** first two cases were confirmed by surgical biopsy to be diffuse large B cell lymphoma,and received radiotherapy combined with chemotherapy,or received only *** third case was confirmed by core needle biopsy to be mucosa-associated lymphoid tissue lymphoma,and received *** summary,confirmation of PTL diagnosis is essential for further clinical *** biopsy should be one of the most important methods to make the diagnosis of PTL,while the use of fine needle aspiration cytology alone is still limited in diagnosing PTL.

关键词： primary thyroid lymphoma B-cell lymphoma Hashimoto’s thyroiditis fine needle aspiration cytology core needle biopsy

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Do medullary thyroid carcinoma patients with high calcitonin require bilateral neck lymph node clearance?A case report

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World Journal of Clinical Cases 2021年第6期9卷 1343-1352页

作者： Feng-Jiao Gan Tie Zhou Shun Wu Meng-Xi Xu Su-Hong Sun Department of Thyroid and Breast Surgery Affiliated Hospital of Zunyi Medical UniversityZunyi 563000Guizhou ProvinceChina

BACKGROUND In clinical work,85%-90%of malignant thyroid diseases are papillary thyroid cancer(PTC);thus,clinicians neglect other types of thyroid cancer,such as medullary thyroid carcinoma(MTC).CASE SUMMARY We report a 53-year-old female patient with a preoperative calcitonin level of 345 pg/*** was no definitive diagnosis of MTC by preoperative fine-needle aspiration cytology or intraoperative frozen pathology,but the presence of PTC and MTC was confirmed by postoperative paraffin *** patient underwent total thyroidectomy and bilateral central lymph node *** follow-up at 1.5 years after surgery revealed no signs of recurrence or *** The issue in clinical work-up regarding types of thyroid cancer provides a novel and challenging idea for the surgical treatment of *** the absence of central lymph node metastasis,it is worth addressing whether patients with high calcitonin can undergo total thyroidectomy and bilateral central lymph node dissection without bilateral lateral neck lymph node dissection.

关键词： Medullary thyroid carcinoma Papillary thyroid carcinoma Simultaneous different types of thyroid cancer Calcitonin fine needle aspiration cytology Surgery Case report

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The diagnostic and prognostic values of plasma Epstein-Barr virus DNA for residual cervical lymphadenopathy in nasopharyngeal carcinoma patients:a retrospective study

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Cancer Communications 2019年第1期39卷 135-147页

作者： Sai-Lan Liu Xue-Song Sun Xiao-Yun Li Lin-Quan Tang Qiu-Yan Chen Huan-Xin Lin Yu-Jing Liang Jin-Jie Yan Chao Lin Shan-Shan Guo Li-Ting Liu Yang Li Hao-Jun Xie Qing-Nan Tang Hu Liang Ling Guo Hai-Qiang Mai State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer MedicineGuangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and TherapySun Yat-sen University Cancer CenterGuangzhou 510060GuangdongP.R.China Department of Nasopharyngeal Carcinoma Sun Yat-sen University Cancer CenterGuangzhou 510060Guang-dongP.R.China Department of Radiation Oncology Sun Yat-sen University Cancer CenterGuangzhou 510060GuangdongP.R.China

Background:Currently,the diagnosis and treatment of nasopharyngeal carcinoma(NPC)patients with residual cervical lymphadenopathy following radical radiotherapy with or without chemotherapy are *** investigated the prognosis of NPC patients with residual cervical lymphadenopathy and assessed the diagnostic and prognostic values of Epstein-Barr virus(EBV)DNA in these ***:This study included 82 NPC patients who were diagnosed with suspected residual cervical lymphadenopathy following completion of antitumor *** plasma EBV DNA levels were measured using quantitative polymerase chain reaction(qPCR)before the initiation of treatment and before neck *** needle aspiration cytology(FNAC)was performed in 21 *** patients had undergone neck dissection and postoperative pathological examination to identify the nature of residual cervical *** overall survival(OS),progression-free survival(PFS),distant metastasis-free survival(DMFS),and locoregional relapse-free survival(LRRFS)were calculated using the Kaplan-Meier method and compared using the log-rank *** Cox proportional hazards model was used to calculate hazard ratios(HRs)with 95%confidence intervals(CIs).Multivariable analysis was used to estimate the effect of potential prognostic factors on ***:Following a median follow-up of 52.6 months,compared with patients with negative postoperative pathological findings for residual cervical lymphadenopathy,the patients with positive findings had a significantly lower 3-year PFS rate(49.9%vs.83.3%,P=0.008).Among NPC patients with residual cervical lymphadenopathy,the patients with preoperative plasma EBV DNA>0 copy/mL had a lower 3-year PFS rate than did those with no detectable EBV DNA(43.7%vs.61.1%,P=0.031).In addition,combining FNAC with preoperative EBV DNA detection improved the diagnostic *** analysis demonstrated that residual cervical lymphadenopathy with positive po

关键词： Nasopharyngeal carcinoma Residual cervical lymphadenopathy Prognosis Epstein-Barr virus fine needle aspiration cytology Survival

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Solitary Thyroid Nodule: Clinical, Sonography and Pathological Evaluation Risk of Malignancy

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International Journal of Otolaryngology and Head & Neck Surgery 2021年第5期10卷 441-476页

作者： Burkan Nasr M. Qubati S. Qubati Abdulhakim Al-Tamimi Yasser A. Rabo Anwar Aljounaeed Abdulfatah Al-Tam Mohmmed Al-Shujaa Mohmmed Al-Shehari Department of Surgery Saudi Hospital at Hajjah Hajjah Yemen Department of Surgery Aden University Aden Yemen Department of Surgery Sana&#224 University Sana&#224 Yemen Department of Pathology Taiz University Taiz Yemen Department of Pathology Thamar University Thamar Yemen Department of Surgery Thamar University Thamar Yemen Department of Surgery 21 September University Sana&#224 Yemen

Aim: To determined risk of increases Incidence of Thyroid cancer in solitary thyroid nodules so Preoperative distinction between benign and malignant in solitary thyroid nodules is important and helps to avoid unnecessary surgery and its adverse effects, such as hypothyroidism, hypocalcemia, and recurrent nerve injury. Methods: Descriptive perspective analyzed data over a period of 6 years April 2015-April 2021 in Saudi Hospital at Hajjah, Yemen. 226 thyroid operations for 207 patients, 135 patient’s diagnosis as Solitary thyroid nodule and 72 patients as Multi nodular goiter. Patients with a clinically solitary thyroid nodule were included in the study group. Results: 135 cases of clinically detected solitary thyroid nodules, 126 female and 9 male patients, between 14 - 65 years age, median 41 years and mean 39.76 years, (94, 41) patients respectively Right side thyroid effect more than Left side, fine needle aspiration cytology (FNAC) sensitivity, specificity and accuracy was (61%, 72%, 64%) respectively. Postoperative histopathology reported 100 (74%) patients as having benign thyroid nodules and 35 patients (26%) as having malignant thyroid nodules. Postoperative transient hypocalcemia was observed in 9 patients (7%), and temporary hoarseness was observed in 3 patients (2%). Conclusion: The incidence of malignancy in solitary thyroid nodules is high. Rapid growth by history and hard fixed nodule by examination and hypoechoic, micro calcification and cervical lymphadenopathy on Thyroid Ultrasound frequently in malignant nodules. Male risk factors for thyroid cancer but age, number and size of nodules not included as risk factor. fine needle aspiration cytology (FNAC) is more helpful for diagnosing if aspiration under Ultrasound guidance and reading by experience histopathologists. The type of surgery depends on preoperative evaluation, including history, examination, ultrasound, fine needle aspir

关键词： Solitary Thyroid Nodule Thyroid Cancer fine needle aspiration cytology Thyroid Ultrasound

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Cytologic patterns of lymph node diseases in Hawassa University Referral Hospital,Southern Ethiopia

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Journal of Coastal Life Medicine 2015年第5期 395-397页

作者： Gemechu Ameya Buli Fekade Yerakly Lukas Department of Medical Laboratory Sciences College of Medicine and Health SciencesArba Minch University.Arba MinchEthiopia School of Medicine College of Medicine and Health SciencesHawassa University.HawassaEthiopia

Objective:To assess the cytological patterns of lymph node diseases in patient attended Hawassa University Referral Hospital,Southern ***:A five years retrospective descriptive study design was conducted on fine needle aspiration cytology reports of patients referred to pathology laboratory of Hawassa University Referral Hospital from September,2009 to September,***:A total of 1067 lymph nodes were aspirated in the study *** lymphadenopathy was the most frequent(48.82%)followed by submandibular(22.77%)lymph *** age group of 11-20 years was the most affected age group while age group above 60 with less *** lymphadenitis was the highest(48.82%),chronic non-specific lymphadenitis(20.33%),reactive(16.21%),pyogenic abscess(5.99%)and the rest were ***:Lymphadenopathy can be associated with a wide range of *** tuberculosis lymphadenitis is the most common cause of enlarged lymph node in the study area.

关键词： fine needle aspiration cytology Lymphadenopathy Lymph node disease

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