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Targeted Lipidomics Studies Reveal that Linolenic Acid Promotes Cotton fiber elongation by Activating Phosphatidylinositol and Phosphatidylinositol Monophosphate Biosvnthesis

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Molecular Plant 2015年第6期8卷 911-921页

作者： Gao-Jun Liu Guang-Hui Xiao Ning-Jing Liu Dan Liu Pei-Shuang Chen Yong-Mei Qin Yu-Xian Zhu The State Key Laboratory of Protein and Plant Gene Research College of Life Sciences Peking University Beijing 100871 China

The membrane lipids from fast-elongating wild-type cotton （Gossypium hirsutum） fibers at 10 days post- anthesis, wild-type ovules with fiber cells removed, and ovules from the fuzzless-lintless mutant harvested at the same age, were extracted, separated, and quantified. fiber cells contained significantly higher amounts of phosphatidylinositol （PI） than both ovule samples with PI 34：3 being the most predominant spe- cies. The genes encoding fatty acid desaturases （415GhFAD）, PI synthase （PIS） and PI kinase （PIK） were expressed in a fiber-preferential manner. Further analysis of phosphatidylinositol monophosphate （PIP） indicated that elongating fibers contained four- to five-fold higher amounts of PIP 34：3 than the ovules. Exog- enously applied linolenic acid （C18：3）, soybean L-α-PI, and PIPs containing PIP 34：3 promoted significant fiber growth, whereas a liver PI lacking the C18：3 moiety, linoleic acid, and PIP 36：2 were completely ineffec- tive. The growth inhibitory effects of carbenoxolone, 5-hydroxytryptamine, and wortmannin were reverted by C18：3, PI, or PIP, respectively, suggesting that PIP signaling is essential for fiber cell growth. Furthermore, cotton plants expressing virus-induced gene-silencing constructs that specifically suppressed Gh15FAD, GhPIS, or GhPIK expression, resulted in significantly short-fibered phenotypes. Our data provide the basis for in-depth studies on the roles of PI and PIP in mediating cotton fiber growth.

关键词： Gossypium hirsutum fiber elongation mass spectrometry glycerophospholipid profiling phosphati-dylinositol phosphatidylinositol monophosphate

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Strigolactones modulate cotton fiber elongation and secondary cell wall thickening

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Journal of Integrative Agriculture 2024年第6期23卷 1850-1863页

作者： Yunze Wen Peng He Xiaohan Bai Huizhi Zhang Yunfeng Zhang Jianing Yu College of Life Sciences Shaanxi Normal UniversityXi’an 710119China

Cotton is one of the most important economic crops in the world,and it is a major source of fiber in the textile ***(SLs)are a class of carotenoid-derived plant hormones involved in many processes of plant growth and development,although the functions of SL in fiber development remain largely ***,we found that the endogenous SLs were significantly higher in fibers at 20 days post-anthesis(DPA).Exogenous SLs significantly increased fiber length and cell wall ***,we cloned three key SL biosynthetic genes,namely GhD27,GhMAX3,and GhMAX4,which were highly expressed in fibers,and subcellular localization analyses revealed that GhD27,GhMAX3,and GhMAX4 were localized in the *** exogenous expression of GhD27,GhMAX3,and GhMAX4 complemented the physiological phenotypes of d27,max3,and max4 mutations in Arabidopsis,*** of GhD27,GhMAX3,and GhMAX4 in cotton resulted in increased numbers of axillary buds and leaves,reduced fiber length,and significantly reduced fiber *** findings revealed that SLs participate in plant growth,fiber elongation,and secondary cell wall formation in *** results provide new and effective genetic resources for improving cotton fiber yield and plant architecture.

关键词： strigolactones fiber elongation secondary cell wall thickening cotton

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GhKNL1 controls fiber elongation and secondary cell wall synthesis by repressing its downstream genes in cotton(Gossypium hirsutum)

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Journal of Integrative Plant Biology 2022年第1期64卷 39-55页

作者： Yao Wang Yang Li Si-Ying Gong Li-Xia Qin Xiao-Ying Nie Dong Liu Yong Zheng Xue-Bao Li Hubei Key Laboratory of Genetic Regulation and Integrative Biology School of Life SciencesCentral China Normal UniversityWuhan 430079China

Cotton which produces natural fiber materials for the textile industry is one of the most important crops in the world. Class II KNOX proteins are often considered as transcription factors in regulating plant secondary cell wall(SCW) formation. However,the molecular mechanism of the KNOX transcription factor-regulated SCW synthesis in plants(especially in cotton) remains unclear in details so far. In this study, we show a cotton class II KNOX protein(Gh KNL1) as a transcription repressor functioning in fiber development. The Gh KNL1-silenced transgeniccotton produced longer fibers with thicker SCWs,whereas Gh KNL1 dominant repression transgenic lines displayed the opposite fiber phenotype, compared with controls. Further experiments revealed that Gh KNL1 could directly bind to promoters of Gh Ces A4-2/4-4/8-2 and Gh MYB46 for modulating cellulose synthesis during fiber SCW development in cotton. On the other hand, Gh KNL1 could also suppress expressions of Gh EXPA2 D/4 A-1/4 D-1/13 A through binding to their promoters for regulating fiber elongation of cotton. Taken together, these data revealed Gh KNL1 functions in fiber elongation and SCW formation by directly repressing expressions of its target genes related to cell elongation and cellulose synthesis. Thus, our data provide an effective clue for potentially improving fiber quality by genetic manipulation of Gh KNL1 in cotton breeding.

关键词： cotton(Gossypium hirsutum) fiber elongation KNOX protein secondary cell wall(SCW)synthesis transcriptional regulation

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GhAlaRP, a cotton alanine rich protein gene, involves in fiber elongation process

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The Crop Journal 2021年第2期9卷 313-324页

作者： Shouhong Zhu Yanjun Li Xinyu Zhang Feng Liu Fei Xue Yongshan Zhang Zhaosheng Kong Qian-Hao Zhu Jie Sun College of Agriculture/The Key Laboratory of Oasis Eco-agriculture Shihezi UniversityShihezi 832000XinjiangChina State Key Laboratory of Cotton Biology Institute of Cotton ResearchAcademy of Agricultural SciencesAnyang 455000HenanChina State Key Laboratory of Plant Genomics Institute of Microbiology Academy of Seed DesignChinese Academy of SciencesBeijing 100101China CSIRO Agriculture and Food GPO Box 1700Canberra 2601Australia

fiber length is one of the most important quality parameters of cotton *** analyses of developing cotton fibers have identified genes preferentially expressing in fiber elongation stage,but few have been functionally ***,on the basis of confirmation of the preferential expression profile of GhAlaRP(Gh_A09G1166 and Gh_D09G1172),an alanine rich protein gene,in the rapid elongating fibers,we investigated the role of GhAlaRP in fiber development by generating transgenic cottons with an increased or decreased expression level of *** results showed that the fiber length was consistently significantly shorter in both the GhAlaRP-RNAi lines and the alarp mutant generated by genome editing than in the control *** was localized on plasma membrane,nucleus and endoplasmic *** yeast two-hybrid assay and bimolecular fluorescence complementation assay showed that GhAlaRP co-expresses and interacts with GhAnnexin(Gh_D11G2184)and GhEXPA(Gh_A10G2323)that are involved in fiber ***-regulation of GhAlaRP co-suppressed the expression levels of GhAnnexin and *** results suggest a role of GhAlaRP in regulation of cotton fiber elongation,which could be achieved by regulating the functions of GhAnnexin and GhEXPA.

关键词： AlaRP fiber elongation G.hirsutum Genome editing

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GhIQD10 interacts with GhCaM7 to control cotton fiber elongation via calcium signaling

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The Crop Journal 2023年第2期11卷 447-456页

作者： Fan Xu Li Wang Jun Xu Qian Chen Caixia Ma Li Huang Guiming Li Ming Luo Key Laboratory of Biotechnology and Crop Quality Improvement of the Ministry of Agriculture Biotechnology Research CenterSouthwest UniversityChongqing 400715China Zhengzhou Research Base State Key Laboratory of Cotton BiologyZhengzhou UniversityZhengzhou 450001HenanChina Academy of Agricultural Sciences of Southwest University State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest UniversityChongqing 400715China

IQ67-domain(IQD)proteins function in plant defense and in organ *** mechanisms by which they influence cotton fiber development are *** the present study,GhIQD10 was expressed mainly in the transition period of cotton fiber development,and GhIQD10-overexpression lines showed shorter ***10 interacted with GhCaM7 and the interaction was inhibited by Ca^(2+).In in vitro ovule culture,Ca^(2+)rescued the shorter-fiber phenotype of GhIQD10-overexpression lines,which were insensitive to the Ca^(2+)channel inhibitor verapamil and the Ca^(2+)pool release channel blocker 2-aminoethoxydiphenyl *** conclude that GhIQD10 affects cotton fiber elongation via Ca^(2+)signaling by interacting with ***(BR)biosynthesis and signaling genes were up-regulated in GhIQD10-overexpression *** development in these lines was not affected by epibrassinolide or the BR biosynthesis inhibitor brassinozole,indicating that the influence of GhIQD10 on fiber elongation was not associated with BR.

关键词： Cotton fiber elongation IQ67-domian protein Ca^(2+) Brassinosteroid

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Cloning and characterization of two putative seven-transmembrane receptor genes from cotton

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Progress in Natural Science:Materials International 2008年第10期18卷 1225-1231页

作者： Peng Gao Piming Zhao Juan Wang Haiyun Wang Guiling Wang Guixian Xia National Key Laboratory of Plant Genomics,Institute of Microbiology,Chinese Academy of Sciences,Datun Road,Chaoyang District,Beijing 100101,China National Center for Plant Gene Research,Beijing 100101,China National Key Laboratory of Plant Genomics Institute of Microbiology Chinese Academy of Sciences Datun Road Chaoyang District Beijing 100101 China National Center for Plant Gene Research Beijing 100101 China

Using rapid amplification of cDNA ends (RACE)-PCR,two full-length cDNAs encoding putative seven-transmembrane receptors (designated Gh7TMpRI and Gh7TMpR2) were cloned from cotton *** blot and an ApaL1 restriction site polymorphism analyses revealed that Gh7TMpRI was derived from the ancestral A diploid genome,while Gh7TMpR2 was from the D subgenome. Northern blot hybridization indicated that both Gh7TMpR1 and GhTTMpR2 were expressed preferentially in the elongation phase of fiber *** of the Gh7TMpRl proteins were located within the membrane structure and displayed a punctuate pattern of *** of Gh7TMpR1 in fission yeast disrupted the polar growth and caused the formation of rounded cells. These results suggest that GhT7MpRl may play a critical role in cotton fiber development,perhaps as a signaling receptor that is involved in controlling fiber elongation.

关键词： Cotton Seven-transmembrane protein fiber elongation Alloalleles

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利用CDNA微阵列分析海陆棉花品种间伸长时间的差异表达谱(英文)

利用CDNA微阵列分析海陆棉花品种间伸长时间的差异表达谱(英文)

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第五次全国植物分子育种代表大会暨学术交流会

作者：陈向东郭旺珍张天真 National Key Laboratory of Crop Genetics and Germplasm Enhancement Cotton Research InstituteNanjing Agricultural University

Sea-island cotton (Gossypium barbadense L.) has superior fiber quality properties such as length,fine- ness and strength,while upland cotton (Gossypium hirsutum L.) is characterized by high *** extensive studies on cotton fiber development,little is known about the cellular and molecular events responsible for cotton fiber elongation and second cell wall synthesis of these two *** technology offers a powerful tool to identify differentially expressed genes at different stages of *** results can be used to deepen our understanding of the molecular mechanisms of cotton fiber *** patterns of a subset of genes during fiber cell elongation and in second wall deposits were established to reveal features of upland cotton and Sea-island cotton fiber cells at the elongation stage. Comparative analysis of transcriptnmes by cDNA microarray showed 501 genes were differentially expressed at the fiber cell elongation *** these,321 genes were up-regulated and 172 genes were down-regulated in TM-1 versus Hai7124,re- *** additional genes were both up-regulated and down-regulated in TM-1 versus Hai7124 during distinctive developmental *** of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the genes differentially expressed indicated the encoding proteins were primarily involved in calcium ion/calmod- ulin binding,cell wall-related enzymes and the response to phytohormone stimulus,or found in signalling ***- tionally,sucrose and starch metabolism,flavonnid biosynthesis,phenylalanine metabolism,ascorbate and aldarate metabo- lism,pyrimidine metabolism and the phosphatidylinositol signalling system were involved in the fiber elongatinn stage.U- sing microarray technology,many novel genes were discovered and a large number of genes differentially expressed during fiber cell elongation were identified between *** ***-1 and *** ***

关键词： expression profiling cotton fiber elongation cDNA microarray

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