GhAlaRP, a cotton alanine rich protein gene, involves in fiber elongation process

作     者：Shouhong Zhu Yanjun Li Xinyu Zhang Feng Liu Fei Xue Yongshan Zhang Zhaosheng Kong Qian-Hao Zhu Jie Sun Shouhong Zhu;Yanjun Li;Xinyu Zhang;Feng Liu;Fei Xue;Yongshan Zhang;Zhaosheng Kong;Qian-Hao Zhu;Jie Sun

作者机构：College of Agriculture/The Key Laboratory of Oasis Eco-agricultureShihezi UniversityShihezi 832000XinjiangChina State Key Laboratory of Cotton BiologyInstitute of Cotton ResearchAcademy of Agricultural SciencesAnyang 455000HenanChina State Key Laboratory of Plant GenomicsInstitute of Microbiology Academy of Seed DesignChinese Academy of SciencesBeijing 100101China CSIRO Agriculture and FoodGPO Box 1700Canberra 2601Australia 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2021年第9卷第2期

页      面：313-324页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：This work was supported by the National Key Research and Development Program of China(2016YFD0101900,2017YFD0100200) the Genetically Modified Organisms Breeding Major Project of China(2016ZX08005-005) 

主　　题：AlaRP Fiber elongation G.hirsutum Genome editing 

摘      要：Fiber length is one of the most important quality parameters of cotton *** analyses of developing cotton fibers have identified genes preferentially expressing in fiber elongation stage,but few have been functionally ***,on the basis of confirmation of the preferential expression profile of GhAlaRP(Gh_A09G1166 and Gh_D09G1172),an alanine rich protein gene,in the rapid elongating fibers,we investigated the role of GhAlaRP in fiber development by generating transgenic cottons with an increased or decreased expression level of *** results showed that the fiber length was consistently significantly shorter in both the GhAlaRP-RNAi lines and the alarp mutant generated by genome editing than in the control *** was localized on plasma membrane,nucleus and endoplasmic *** yeast two-hybrid assay and bimolecular fluorescence complementation assay showed that GhAlaRP co-expresses and interacts with GhAnnexin(Gh_D11G2184)and GhEXPA(Gh_A10G2323)that are involved in fiber ***-regulation of GhAlaRP co-suppressed the expression levels of GhAnnexin and *** results suggest a role of GhAlaRP in regulation of cotton fiber elongation,which could be achieved by regulating the functions of GhAnnexin and GhEXPA.