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The NAC transcription factor LuNAC61 negatively regulates fiber development in flax (Linum usitatissimum L.)

Journal of Integrative Agriculture 2024年第3期23卷 795-805页

作者： Dongwei Xie Jing Li Wan Li Lijun Sun Zhigang Dai Wenzhi Zhou Jianguang Su Jian Sun School of Life Sciences Nantong UniversityNantong 226019China Institute of Crop Cultivation and Tillage Heilongjiang Academy of Agricultural SciencesHarbin 150086China Institute of Bast fiber Crops Chinese Academy of Agricultural SciencesChangsha 410221China Jiangsu Sanshu Biotechnology Co.Ltd. Nantong 226001China

Flax is a crucial fiber crop that exhibits excellent textile properties and serves as a model plant for investigating phloem fiber development. The regulation of multiple genes significantly influences fiber development, notably involving NAC(NAM, ATAF1/2, CUC2) transcription factors in forming the fiber secondary cell wall(SCW).Overexpression of LuNAC61 in flax resulted in sparse top meristematic zone leaves and significantly reduced stem cellulose content. Scanning electron microscopy and staining observations revealed a significant reduction in fiber bundles. β-Glucuronidase(GUS) staining analysis demonstrated high activity of the LuNAC61 promoter in the bast fibers of the flax stem. Additionally, several members of the LuPLATZ and LuCesA families exhibited significant coexpression with LuNAC61. Subcellular localization indicated the presence of LuPLATZ24 protein in the nucleus and cytoplasm, LuNAC61 protein exclusively in the nucleus, and LuCesA10 in the nucleus and endoplasmic reticulum. LuPLATZ24 positively regulates LuNAC61, whereas LuNAC61 negatively affects LuCesA10, suggesting the involvement of a metabolic network in regulating flax fiber development. In conclusion, this study provides a critical opportunity for a comprehensive and in-depth analysis of the mechanisms governing flax fiber development and the potential use of biotechnology to enhance flax fiber yield.

关键词： flax fiber development Lu NAC61 gene function gene interaction

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Comparative phosphoproteomic analysis of BR-defective mutant reveals a key role of GhSK13 in regulating cotton fiber development

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Science China(Life Sciences) 2020年第12期63卷 1905-1917页

作者： Lingling Wang Han Cheng Fangjie Xiong Shuya Ma Lei Zheng Yun Song Kexuan Deng Huanhuan Wu Fuguang Li Zuoren Yang Zhengzhou Research Base State Key Laboratory of Cotton BiologyZhengzhou UniversityZhengzhou 450001China State Key Laboratory of Cotton Biology Institute of Cotton ResearchChinese Academy of Agricultural SciencesAnyang 455000China Key Laboratory for Ecology of Tropical Islands Ministry of EducationCollege of Life SciencesHainan Normal UniversityHaikou 571158China School of Life Sciences Chongqing UniversityChongqing 401331China

Brassinosteroid(BR), a steroid phytohormone, whose signaling transduction pathways include a series of phosphorylation and dephosphorylation events, and GSK3 s are the main negative regulator kinases. BRs have been shown to play vital roles in cotton fiber elongation. However, the underlying mechanism is still elusive. In this study, fibers of a BR-defective mutant Pagoda 1(pag1), and its corresponding wild-type(ZM24) were selected for a comparative global phosphoproteome analysis at critical developmental time points: fast-growing stage(10 days after pollination(DPA)) and secondary cell wall synthesis stage(20DPA). Based on the substrate characteristics of GSK3, 900 potential substrates were identified. Their GO and KEGG annotation results suggest that BR functions in fiber development by regulating GhSKs(GSK3s of Gossypium hirsutum L.) involved microtubule cytoskeleton organization, and pathways of glucose, sucrose and lipid metabolism. Further experimental results revealed that among the GhSK members identified, GhSK13 not only plays a role in BR signaling pathway, but also functions in developing fiber by respectively interacting with an AP2-like ethylene-responsive factor GhAP2L, a nuclear transcription factor GhDNFYB19, and a homeodomain zipper member GhHDZ5. Overall, our phosphoproteomic research advances the understanding of fiber development controlled by BR signal pathways especially through GhSKs, and also offers numbers of target proteins for improving cotton fiber quality.

关键词： phosphoproteome brassinosteroid GSK3 GhSK13 cotton fiber development

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A genome-wide identification of the BLH gene family reveals BLH1 involved in cotton fiber development

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Journal of Cotton Research 2020年第3期3卷 199-209页

作者： LIU Cuixia LI Zhifang DOU Lingling YUAN Yi ZOU Changsong SHANG Haihong CUI Langjun XIAO Guanghui Key Laboratory of the Ministry of Education for Medicinal Plant Resources and Natural Pharmaceutical Chemistry National Engineering Laboratory for Resouce Development of Endangered Crude Drugs in the Northwest of ChinaCollege of Life SciencesShaanxi Normal UniversityXi’an 710119ShaanxiChina College of Biology Pharmacy and Food Engineering Shangluo UniversityShangluo 726000ShaanxiChina State Key Laboratory of Cotton Biology State Key Laboratory of Crop Stress Adaptation and ImprovementSchool of Life SciencesHenan UniversityKaifeng 475001HenanChina School of Chemistry and Chemical Engineering Xianyang Normal UniversityXianyang 712000ShaanxiChina Zhengzhou Research Base State Key Laboratory of Cotton BiologyZhengzhou UniversityZhengzhou 450001China

Background:Cotton is the world’s largest and most important source of renewable natural fiber.BEL1-like homeodomain(BLH)genes are ubiquitous in plants and have been reported to contribute to plant development.However,there is no comprehensive characterization of this gene family in cotton.In this study,32,16,and 18 BLH genes were identified from the G.hirsutum,G.arboreum,and G.raimondii genome,respectively.In addition,we also studied the phylogenetic relationships,chromosomal location,gene structure,and gene expression patterns of the BLH genes.Results:The results indicated that these BLH proteins were divided into seven distinct groups by phylogenetic analysis.Among them,25 members were assigned to 15 chromosomes.Furthermore,gene structure,chromosomal location,conserved motifs,and expression level of BLH genes were investigated in G.hirsutum.Expression profiles analysis showed that four genes(GhBLH1_3,GhBLH1_4,GhBLH1_5,and GhBLH1_6)from BLH1 subfamily were highly expressed during the fiber cell elongation period.The expression levels of these genes were significantly induced by gibberellic acid and brassinosteroid,but not auxin.Exogenous application of gibberellic acid significantly enhanced GhBLH1_3,GhBLH1_4,and GhBLH1_5 transcripts.Expression levels of GhBLH1_3 and GhBLH1_4 genes were significantly increased under brassinosteroid treatment.Conclusions:The BLH gene family plays a very important role in many biological processes during plant growth and development.This study deepens our understanding of the role of the GhBLH1 gene involved in fiber development and will help us in breeding better cotton varieties in the future.

关键词： Cotton G.hirsutum BLH gene family fiber development

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A comprehensive overview of cotton genomics,biotechnology and molecular biological studies

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Science China(Life Sciences) 2023年第10期66卷 2214-2256页

作者： Xingpeng Wen Zhiwen Chen Zuoren Yang Maojun Wang Shuangxia Jin Guangda Wang Li Zhang Lingjian Wang Jianying Li Sumbul Saeed Shoupu He Zhi Wang Kun Wang Zhaosheng Kong Fuguang Li Xianlong Zhang Xiaoya Chen Yuxian Zhu Institute for Advanced Studies Wuhan UniversityWuhan 430072China College of Life Sciences Wuhan UniversityWuhan 430072China National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesUniversity of CASChinese Academy of SciencesShanghai 200032China Hainan Yazhou Bay Seed Laboratory Sanya 572025China State Key Laboratory of Cotton Biology Institute of Cotton ResearchChinese Academy of Agricultural SciencesAnyang 455000China Hubei Hongshan Laboratory National Key Laboratory of Crop Genetic ImprovementCollege of Plant Science and TechnologyHuazhong Agricultural UniversityWuhan 430070China State Key Laboratory of Plant Genomics Institute of MicrobiologyInnovative Academy of Seed DesignChinese Academy of SciencesBeijing 100101China Shanxi Agricultural University Jinzhong 030801China

Cotton is an irreplaceable economic crop currently domesticated in the human world for its extremely elongated fiber cells specialized in seed epidermis,which makes it of high research and application value.To date,numerous research on cotton has navigated various aspects,from multi-genome assembly,genome editing,mechanism of fiber development,metabolite biosynthesis,and analysis to genetic breeding.Genomic and 3D genomic studies reveal the origin of cotton species and the spatiotemporal asymmetric chromatin structure in fibers.Mature multiple genome editing systems,such as CRISPR/Cas9,Cas12(Cpf1)and cytidine base editing(CBE),have been widely used in the study of candidate genes affecting fiber development.Based on this,the cotton fiber cell development network has been preliminarily drawn.Among them,the MYB-b HLH-WDR(MBW)transcription factor complex and IAA and BR signaling pathway regulate the initiation;various plant hormones,including ethylene,mediated regulatory network and membrane protein overlap fine-regulate elongation.Multistage transcription factors targeting Ces A 4,7,and 8 specifically dominate the whole process of secondary cell wall thickening.And fluorescently labeled cytoskeletal proteins can observe real-time dynamic changes in fiber development.Furthermore,research on the synthesis of cotton secondary metabolite gossypol,resistance to diseases and insect pests,plant architecture regulation,and seed oil utilization are all conducive to finding more high-quality breeding-related genes and subsequently facilitating the cultivation of better cotton varieties.This review summarizes the paramount research achievements in cotton molecular biology over the last few decades from the above aspects,thereby enabling us to conduct a status review on the current studies of cotton and provide strong theoretical support for the future direction.

关键词： cotton genome assembly genome editing fiber development gossypol plant architecture oil utilization genetic breeding

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Comparative genomic analyses reveal cis-regulatory divergence after polyploidization in cotton

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The Crop Journal 2022年第6期10卷 1545-1556页

作者： Jiaqi You Min Lin Zhenping Liu Liuling Pei Yuexuan Long Lili Tu Xianlong Zhang Maojun Wang National Key Laboratory of Crop Genetic Improvement Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070HubeiChina School of Pharmaceutical Sciences South-Central University for NationalitiesWuhan 430074HubeiChina

Polyploidization has long been recognized as a driver for the evolutionary formation of superior plant traits coupled with gene expression novelty.However,knowledge of the effect of regulatory variation on expression changes following polyploidization remains limited.In this study,we characterized transcriptional regulatory divergence by comparing tetraploid cotton with its putative diploid ancestors.We identified 144,827,99,609,and 219,379 Tn5 transposase-hypersensitive sites(THSs)in Gossypium arboreum,G.raimondii,and G.hirsutum,respectively,and found that the conservation of promoter THSs was associated with coordination of orthologous genes expression.This observation was consistent with analysis of transcription-factor binding sites(TFBS)for 262 known motifs:genes with higher TFBS conservation scores(CS)showed less change than those genes with lower TFBS CS in expression levels.TFBS influenced by genomic variation were involved in the novel regulation networks between transcriptional factors and target genes in tetraploid cotton.We describe an example showing that the turnover of TFBS was linked to expression pattern divergence of genes involved in fiber development(fiber-related genes).Our findings reveal the regulatory divergence of the transcriptional network in cotton after polyploidization and characterizes the regulatory relationships of genes contributing to desirable traits.

关键词： Cotton Polyploidization Transcriptional regulation fiber development

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Cotton PIP2 proteins selectively interact to regulate their water channel activities and are required for fiber development

Cotton PIP2 proteins selectively interact to regulate their ...

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第十四届全国植物基因组学大会

作者： Dengdi Li Jie Zhang XM Ruan Fang Luo Ying Li Xuebao Li Hubei Key Laboratory of Genetic Regulation and Integrative Biology College of Life SciencesCentral China Normal University

Aquaporins are small channel proteins present in plasma and intracellular membranes of plant cells,facilitating the transport of water and/or small neutral solutes.Among aquaporins,plasma membrane intrinsic proteins(PIPs) constitute a plasma-membrane specific subfamily and they are further distinguished as PIPl and PIP2 groups.Recent

关键词： cotton（Gossypium hirsutum） fiber development aquaporin coexpression suppression of gene expression protein-protein interaction

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A Genome-Scale Analysis of the PIN Gene Family Reveals Its Functions in Cotton fiber development

A Genome-Scale Analysis of the PIN Gene Family Reveals Its F...

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中国农学会棉花分会2017年年会暨第九次会员代表大会

作者： Zhang Yuzhou He Peng Yang Zuoren Huang Gai Wang Limin Pang Chaoyou Xiao Hui Zhao Peng Yu Jianing Xiao Guanghui College of Life Sciences Shaanxi Normal University Institute of Cotton Research of Chinese Academy of Agricultural Sciences Peking University

The PIN-FORMED(PIN) protein,the most important polar auxin transporter,plays a critical role in the distribution of auxin and controls multiple biological processes.However,characterizations and functions of this gene family have not been identified in cotton.Here,we identified the PIN gene family in Gossypium hirsutum,G.arboreum and G.raimondii.This gene family was divided into seven subgroups.A chromosomal distribution analysis showed that GhPIN genes were evenly distributed in eight chromosomes and that the whole genome and dispersed duplications were the main duplication events for GhPIN expansion.qRT-PCR analysis showed a tissue-specific expression pattern for GhPIN.Likely due to the cis-element variations in their promoters,transcripts of PIN6 and PIN8 genes from the At(tetraploid genome orginated from G.arboreum) subgenome and PIN1 a from the Dt(tetraploid genome orginated from G.raimondii) subgenome in G.hirsutum was significantly increased compared with the transcripts in the diploids.The differential regulation of these PIN genes after the polyploidization may be conductive to fiber initiation and elongation.Exogenously applied auxin polar transport inhibitor significantly suppressed fiber growth,which is consistent with the essential function of these PIN genes in regulating cotton fiber development.Furthermore,the overexpression of GhPIN1at,GhPIN6t,and GhPIN8t in Arabidopsis promoted the density and length of trichomes in leaves.

关键词： cotton PIN-formed auxin efflux carriers fiber development expression patterns

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Cotton AnnGh3 Encoding an Annexin Protein is Preferentially Expressed in fibers and Promotes Initiation and Elongation of Leaf Trichomes in Transgenic Arabidopsis

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Journal of Integrative Plant Biology 2013年第10期55卷 902-916页

作者： Bing Li Deng-Di Li Jie Zhang Hui Xia Xiu-Lan Wang Ying Li Xue-Bao Li Hubei Key Laboratory of Genetic Regulation and Integrative Biology College of Life Sciences Central China Normal University

The annexins are a multifamily of calcium-regulated phospholipid-binding proteins. To investigate the roles of annexins in fiber development, four genes encoding putative annexin proteins were isolated from cotton （Gossypium hirsutum） and designated AnnGh3, AnnGh4, AnnGh5, and AnnGh6. Quantitative reverse transcription-polymerase chain reaction （qRT-PCR） results indicated that AnnGh3, AnnGh4, and AnnGh5 were preferentially expressed in fibers, while the transcripts of AnnGh6 were predominantly accumulated in roots. During fiber development, the transcripts of AnnGh3/4/5 genes were mainly accumulated in rapidly elongating fibers. With fiber cells further developed, their expression activity was dramatically declined to a relatively low level. In situ hybridization results indicated that AnnGh3 and AnnGh5were expressed in initiating fiber cells （0-2 DPA）. Additionally, their expression in fibers was also regulated by phytohormones and [Ca2~]. Subcellular localization analysis discovered that AnnGh3 protein was localized in the cytoplasm. Overexpression of AnnGh3 in Arabidopsis resulted in a significant increase in trichome density and length on leaves of the transgenic plants, suggesting that AnnGh3 may be involved in fiber cell initiation and elongation of cotton.

关键词： Annexin cotton （Gossypium hirsutum） fiber development gene expression.

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Expression patterns and functional divergence of homologous genes accompanied by polyploidization in cotton(Gossypium hirsutum L.)

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Science China(Life Sciences) 2020年第10期63卷 1565-1579页

作者： Zhen Peng Hua Cheng Gaofei Sun Zhaoe Pan Xiao Wang Xiaoli Geng Shoupu He Xiongming Du Research Base Anyang Institute of TechnologyState Key Laboratory of Cotton Biology/Institute of Cotton ResearchChinese Academy of Agricultural Science(ICRCAAS)Anyang 455000China Zhengzhou Research Base State Key Laboratory of Cotton BiologyZhengzhou UniversityZhengzhou 450001China Anyang Institute of Technology Anyang 455000China

Naturally allotetraploid cotton has been widely used as an ideal model to investigate gene expression remodeling as a consequence of polyploidization.However,the global gene pattern variation during early fiber development was unknown.In this study,through RNA-seq technology,we comprehensively investigated the expression patterns of homologous genes between allotetraploid cotton(G.hirsutum)and its diploid progenitors(G.arboreum and G.raimondii)at the fiber early development stage.In tetraploid cotton,genes showed expression level dominance(ELD)bias toward the A genome.This phenomenon was explained by the up-/downregulation of the homologs from the nondominant progenitor(D genome).Gene ontology(GO)enrichment results indicated that the ELD-A genes might be a prominent cause responsible for fiber property change through regulating the fatty acid biosynthesis/metabolism and microtubule procession,and the ELD-D genes might be involved in transcription regulation and stress inducement.In addition,the number and proportion of completely A-and D-subfunctionalized gene were similar at different fiber development stages.However,for neofunctionalization,the number and proportion of reactivated D-derived genes were greater than those of A at 3 and 5 DPA.Eventually,we found that some homologous genes belonging to several specific pathways might create novel asymmetric transcripts between two subgenomes during polyploidization and domestication process,further making the fiber property meet the human demands.Our study identified determinate pathways and their involved genes between allotetraploid cotton and their progenitors at early fiber development stages,providing new insights into the mechanism of cotton fiber evolution.

关键词： Gossypium fiber development polyploid evolution expression level dominance sub-and neofunctionalization

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Molecular Cloning and Characterization of an Allene Oxide Cyclase Gene Associated with fiber Strength in Cotton

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Journal of Integrative Agriculture 2014年第10期13卷 2113-2121页

作者： WANG Li-man ZHU You-min TONG Xiang-chao HU Wen-jing CAI Cai-ping GUO Wang-zhen State Key Laboratory of Crop Genetics & Germplasm Enhancement Hybrid Cotton R&D Engineering Research CenterMinistry of Education/College of AgricultureNanjing Agricultural University

Allene oxide cyclase （AOC） is one of the most important enzymes in the biosynthetic pathway of the plant hormone jasmonic acid （JA）. AOC catalyzes the conversion ofallene oxide into 12-oxo-phytodienoic acid （OPDA）, a precursor of JA. Using 28K cotton genome array hybridization, an expressed sequence tag （EST; GenBank accession no. ES792958） was investigated that exhibited significant expression differences between lintless-fuzzless XinWX and linted-fuzzless XinFLM isogenic lines during fiber initiation stages. The EST was used to search the Gossypium EST database （http：//www.***.nlm.***.gov/） for corresponding cDNA sequences encoding full-length open reading frames （ORFs）. Identified ORFs were confirmed using transcriptional and genomic data. As a result, a novel gene encoding AOC in cotton （Gossypium hirsutum AOC; GenBank accession no. KF383427） was cloned and characterized. The 741-bp GhAOC gene comprises three exons and two introns and encodes a polypeptide of 246 amino acids. Two homologous copies were identified in the tetraploid cotton species G. hirsutum acc. TM-1 and G. barbadense cv. Hai7124, and one copy in the diploid cotton species G. herbaceum and G. raimondii, qRT-PCR showed that the GhAOC transcript was abundant in cotton fiber tissues from 8 to 23 days post anthesis （DPA）, and the expression profiles were similar in the two cultivated tetraploid cotton species G. hirsutum acc. TM- 1 and G. barbadense cv. Hai7124, with a higher level of transcription in the former. One copy of GhAOC in tetraploid cotton was localized to chromosome 24 （Chr. D8） using the subgenome-specific single nucleotide polymorphism （SNP） marker analysis, which co-localized GhAOC to within 10 cM of a fiber strength quantitative trait locus （QTL） reported previously. GhAOC was highly correlated with fiber quality and strength （P=0.014） in an association analysis, suggesting a possible role in cotton fiber development, especially in secondary cell wall thickening.

关键词： allene oxide cyclase structure expression pattern fiber development association analysis cotton

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