Comparative phosphoproteomic analysis of BR-defective mutant reveals a key role of GhSK13 in regulating cotton fiber development

作     者：Lingling Wang Han Cheng Fangjie Xiong Shuya Ma Lei Zheng Yun Song Kexuan Deng Huanhuan Wu Fuguang Li Zuoren Yang Lingling Wang;Han Cheng;Fangjie Xiong;Shuya Ma;Lei Zheng;Yun Song;Kexuan Deng;Huanhuan Wu;Fuguang Li;Zuoren Yang

作者机构：Zhengzhou Research BaseState Key Laboratory of Cotton BiologyZhengzhou UniversityZhengzhou 450001China State Key Laboratory of Cotton BiologyInstitute of Cotton ResearchChinese Academy of Agricultural SciencesAnyang 455000China Key Laboratory for Ecology of Tropical IslandsMinistry of EducationCollege of Life SciencesHainan Normal UniversityHaikou 571158China School of Life SciencesChongqing UniversityChongqing 401331China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2020年第63卷第12期

页      面：1905-1917页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：supported by the National Natural Science Foundation of China(31971987 and 31601067) 

主　　题：phosphoproteome brassinosteroid GSK3 GhSK13 cotton fiber development 

摘      要：Brassinosteroid(BR), a steroid phytohormone, whose signaling transduction pathways include a series of phosphorylation and dephosphorylation events, and GSK3 s are the main negative regulator kinases. BRs have been shown to play vital roles in cotton fiber elongation. However, the underlying mechanism is still elusive. In this study, fibers of a BR-defective mutant Pagoda 1(pag1), and its corresponding wild-type(ZM24) were selected for a comparative global phosphoproteome analysis at critical developmental time points: fast-growing stage(10 days after pollination(DPA)) and secondary cell wall synthesis stage(20DPA). Based on the substrate characteristics of GSK3, 900 potential substrates were identified. Their GO and KEGG annotation results suggest that BR functions in fiber development by regulating GhSKs(GSK3s of Gossypium hirsutum L.) involved microtubule cytoskeleton organization, and pathways of glucose, sucrose and lipid metabolism. Further experimental results revealed that among the GhSK members identified, GhSK13 not only plays a role in BR signaling pathway, but also functions in developing fiber by respectively interacting with an AP2-like ethylene-responsive factor GhAP2L, a nuclear transcription factor GhDNFYB19, and a homeodomain zipper member GhHDZ5. Overall, our phosphoproteomic research advances the understanding of fiber development controlled by BR signal pathways especially through GhSKs, and also offers numbers of target proteins for improving cotton fiber quality.