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Bioengineering models of female reproduction

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Bio-Design and Manufacturing 2020年第3期3卷 237-251页

作者： Maria EZubizarreta Shuo Xiao Department of Environmental Health Sciences Arnold School of Public HealthUniversity of South CarolinaColumbiaSC 29208USA Department of Pharmacology and Toxicology Ernest Mario School of PharmacyEnvironmental Health Sciences InstituteRutgers University170 Frelinghuysen RoadPiscatawayNJ 08854USA

The female reproductive system consists of the ovaries,the female gonads,and the reproductive tract organs of the fallopian tubes,uterus,cervix,and *** functions to provide hormonal support and anatomical structure for the production of new offspring.A number of endogenous and exogenous factors can impact female reproductive health and fertility,including genetic vulnerability,medications,environmental exposures,age,nutrition,and *** date,due to the ethical concerns of using human subjects in biomedical research,the majority of studies use in vivo animal models and 2D cell/tissue culture models to study female ***,the complexity and species difference of the female reproductive system in humans make it difficult to compare to those of ***,the monolayered cells cultured on flat plastics or glass lose their 3D architecture as well as the physical and/or biochemical contacts with other cells in ***,all reproductive organs do not work alone but interconnect with each other and also with non-reproductive organs to support female reproductive,endocrine,and systemic *** facts suggest that there is an urgent and unmet need to develop representative,effective,and efficient in vitro models for studying human female *** prodigious advancements of bioengineering(e.g.,biomaterials,3D printing,and organ-on-a-chip)allow us to study female reproduction in an entirely new ***,we review recent advances that use bioengineering methods to study female reproduction,including the bioengineering models of the ovary,fallopian tube,uterus,embryo implantation,placenta,and reproductive disease.

关键词： female reproduction Bioengineering Biomaterials 3D printing Microfluidics

Sexual size dimorphism and female reproduction in the white-striped grass lizard Takydromus wolteri

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Current Zoology 2012年第2期58卷 236-243页

作者： Laigao LUO Yilian WU Zhuyuan ZHANG Xuefeng XU School of Biology ＆ Food Engineering Chuzhou University Chuzhou Anhui 239000 China

Sexual size dimorphism （SSD） has long attracted the attention of biologists, and life-history variation is thought to play an important role in the evolution of SSD. Here we quantified SSD and female reproductive traits to identify potential asso- ciations between SSD and female reproduction in the white-striped grass lizard Takydromus wolteri. In a population from Chuzhou, China, the largest male and female were 53.0 mm and 57.5 mm in snout-vent length （SVL）, respectively. females were larger in SVL and abdomen length, whereas males were larger in head size and tail length. females produced up to five clutches of eggs during the breeding season, with large females producing more clutches and more eggs per clutch than small ones. As a result, large females had a higher annual fecundity and reproductive output. Egg size was positively correlated with matemal SVL in the first clutch, but not in subsequent clutches. These results suggest that T. wolteri is a species with female-biased SSD, and that fectmdity selection, in which large females have higher fecundity due to their higher capacity for laying eggs, is likely correlated with the evolution of SSD in this species

关键词： Reptilia Lacertidae Takydromus wolteri Sexual size dimorphism female reproduction

Fidgetin knockdown and knockout influences female reproduction distinctly in mice

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The Journal of Biomedical Research 2022年第4期36卷 269-279页

作者： Cong-Rong Li Ruo-Lei Wang Shi-Ya Xie Yan-Ru Li Lei-Lei Gao Zhi-Xia Yang Dong Zhang State Key Lab of Reproductive Medicine Nanjing Medical UniversityNanjingJiangsu 211166China Animal Core Facility Nanjing Medical UniversityNanjingJiangsu 211166China

Microtubule-severing proteins(MTSPs),are a family of proteins which use adenosine triphosphate to sever *** have been shown to play an important role in multiple microtubule-involved cellular *** member of this family,fidgetin(FIGN),is also involved in male fertility;however,no studies have explored its roles in female *** this study,we found mouse fidgetin is rich within oocyte zona pellucida(ZP)and is the only MTSP member to do *** also appears to interact with all three ZP *** findings prompted us to propose that fidgetin might prevent *** from in vitro maturation oocytes analysis showed that fidgetin knockdown did cause *** then deleted all three fidgetin isoforms with CRISPR/Cas9 technologies;however,female mice remained healthy and with normal *** all mouse MTSPs,only the mRNA level of fidgetin-like 1(FIGNL1)significantly ***,we assert that fidgetin-like 1 compensates fidgetin's roles in fidgetin knockout female mice.

关键词： mouse fidgetin knockdown knockout female reproduction

Effects of Mycoestrogens on female reproduction

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Reproductive and Developmental Medicine 2018年第1期2卷 52-58页

作者： Christian Lee Andersen Fei Zhao Xiao-Qin Ye Department of Physiology and Pharmacology College of Veterinary MedicineUniversity of GeorgiaAthensGA 30602USA Interdisciplinary Toxicology Program University of GeorgiaAthensGA 30602USA Reproductive Developmental Biology Group National Institute of Environmental Health SciencesDurhamNC 27709USA

Zearalenone(ZEA)is produced by Fusarium species and a common contaminant in *** and its metabolites,α-andβ-zearalenol,α-andβ-zearalanol,and zearalanone,are mycoestrogens that can interfere with estrogen *** levels of mycoestrogens reduced female fertility in farm animals and rodents,in which adverse effects of mycoestrogens on major events in female reproduction,including ovarian folliculogenesis,ovulation,ovarian steroidogenesis,fertilization,preimplantation embryo development and transport,embryo implantation,placentation,parturition,and lactation,have been reported in different experimental ***,we review the in vivo effects of mycoestrogens on the main events in female reproduction.

关键词： female reproduction Mycoestrogens Puberty Zearalenone

ERK1/2 Activities Are Dispensable for Oocyte Growth but Are Required for Meiotic Maturation and Pronuclear Formation in Mouse

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Journal of Genetics and Genomics 2015年第9期42卷 477-485页

作者： Yin-Li Zhang Xiao-Man Liu Shu-Yan Ji Qian-Qian Sha Jue Zhang Heng-Yu Fan Life Sciences Institute Zhejiang University

Previous studies revealed that extracellular regulated kinase-1 and -2 （ERK1/2） cascade plays pivotal roles in regulating oocyte meiotic cell cycle progression. However, most knowledge about the in vivo function of ERK1/2 in mammalian oocytes was indirectly obtained from analyzing the phenotypes of Mos knockout mice. In this study, we knocked out Erkl and Erk2 in mouse oocytes as early as the primordial follicle stage using the well-characterized Gdp-Cre mouse model, and for the first time directly investigated the in vivo function of ERK1/2 in mouse oocytes. In this novel mouse model, we observed that ERK1/2 activities in oocyte are dispensable for primordial follicle maintenance, activation and follicle growth. Different from the Mos null oocytes, the ERK1/2-deleted oocytes had well-assembled spindles at metaphase I （MI）, extruded polar body-I （PB1） with normal sizes, and did not undergo a full parthenogenetic activation characterized for pronuclear formation. However, the ovulated ERK1/2-deleted oocytes had poorly-assembled metaphase II （MII） spindles, spontaneously released polar body-2 （PB2）, and were arrested at another metaphase called metaphase III （MIII）. In addition, ERK1/2 deletion prevented male pronuclear formation after fertilization, and caused female infertility. In conclusion, these results indicate that ERK1/2 activities are required for not only MII-arrest maintenance, but also efficient pronuclear formation in mouse oocytes.

关键词： Oocyte female reproduction Meiosis ERK1/2 Fertilization

Identification of G protein-coupled receptors required for vitellogenesis and egg development in an insect with panoistic ovary

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Insect Science 2021年第4期28卷 1005-1017页

作者： Hongyuan Zheng Baojuan Zeng Tiantian Shang Shutang Zhou State Key Laboratory of Cotton Biology Key Laboratory of Plant Stress BiologySchool of Life SciencesHenan UniversityKaifengHenan ProvinceChina

G protein-coupled receptors(GPCRs),a superfamily of integral transmembrane proteins regulate a variety of physiological processes in *** hormone(JH)is known to stimulate Vitellogenin(Vg)synthesis in the fat body,secretion into the hemolymph and uptake by developing ***,the role of GPCRs in JH-dependent insect vitellogenesis and oocyte maturation remains *** the present study,we performed transcriptomic analysis and RNA interference(RNAi)screening in vitellogenic females of the migratory locust Locusta *** 22 GPCRs identified in ovarian transcriptome,LGR4,OR-A1,OR-A2,Mthl1,Mthl5 and Smo were most abundant in the *** comparison,mAChR-C expressed at higher levels in the fat body,whereas Oct/TyrR,OARβ,AdoR and ADGRA3 were at higher expression levels in the *** RNAi screening demonstrated that knockdown of six GPCRs resulted in defective phenotypes of Vg accumulation in developing oocytes,accompanied by blocked ovarian development and impaired oocyte *** LGR4 and Oct/TyrR appeared to control Vg synthesis in the fat body,OR-A1,OR-A2,mAChR-C and CirlL regulated Vg transportation and *** findings provide fundamental evidence for deciphering the regulatory mechanisms of GPCRs in JH-stimulated insect reproduction.

关键词： female reproduction GPCR juvenile hormone RNAi transcriptome

Body Size and Reproductive Tactics in Varanid lizards

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Asian Herpetological Research 2014年第4期5卷 263-270页

作者： Yu DU Longhui LIN Yuntao YAO Chixian LIN Xiang JI Hangzhou Key Laboratory for Animal Adaptation and Evolution School of Life Sciences Hangzhou Normal University Hainan Key Laboratory for Herpetology School of Life Sciences Qiongzhou University Jiangsu Key Laboratory for Biodiversity and Biotechnology College of Life Sciences Nanjing Normal University

Body size and female reproduction in the water adult females larger than 500 mm SVL and 32 adult males Ledong, Hainan under permit to our laboratory in Hainan monitor lizard （Varanus salvator） were studied. Forty-two larger than 400 mm SVL were donated by local people in in 2013 and 2014. The largest male and female measured 745 and 755 mm SVL, respectively. The mean SVL was greater in adult females than in adult males. Males had larger heads （head width） than females of the same SVL. The smallest reproductive female in our sample was 565 mm SVL. females produced a single clutch of 17.1 （10-23） pliable-shelled eggs per breeding season stretching from mid-June and mid-September. Clutch size and clutch mass were all positively related to female SVL. However, there was no significant linear relationship between egg mass and female SVL. Larger females generally produced more eggs, and thus heavier clutches than did smaller ones. There was no significant linear relationship between relative clutch mass and female SVL. Phylogenetic generalized least squares （PGLS） analysis, accounting for phylogenetic relationships, showed that clutch size was positively correlated with mean maternal SVL in varanid lizards. PGLS analysis showed that phylogenetic relationships did not affect clutch （or/and egg） mass and the SVL although there were significant linear relationship between clutch （or/and egg） mass and mean maternal SVL. Therefore, we could draw some general conclusions about the body size and reproductive tactics in varanid lizards that larger females generally produced more eggs, larger eggs and thus heavier clutches than did smaller ones.

关键词： body size female reproduction monitor lizard Varanidae

Urinary Bisphenol A concentrations are correlated with poorer oocyte retrieval and embryo implantation outcomes in women with tubal factor infertility in in-vitro fertilization

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Urinary Bisphenol A concentrations are correlated with poore...