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Development of a loop‑mediated isothermal amplification assay for detection of Austropeplea tomentosa from environmental water samples

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Animal Diseases 2023年第1期3卷 35-48页

作者： Lily Tran Vignesh A.Rathinasamy Travis Beddoe Department of Animal Plant and Soil SciencesSchool of AgricultureBiomedicine and EnvironmentLa Trobe UniversityBundooraVIC 3083Australia Full list of author information is available at the end of the article

Lymnaeid snails are key intermediate hosts for the development and survival of fasciola spp.,the causative agent of Fascioliasis which are economically important parasites infecting humans and livestock *** current control method for treating Fascioliasis is heavily reliant on anthelmintic drugs,particularly Triclabendazole(TCBZ)which has resulted in drug-resistant parasites and poses significant risk as there are no long-term efficacious alternatives *** control measures at the farm level could include both parasite and snail control will play an important role in fasciola *** and reduce the reliance on anthelmintic *** of such sustainable control measures requires effective identification of snails on the property however Lymnaeid snails are small and difficult to physically *** identification using an environmental DNA approach is a recent approach in which physically locating snails are not *** tomentosa,is the primary intermediate snail host for *** transmission in South-East Australia and we present an in-field loop-mediated isothermal amplification and water filtering method for the detection of *** eDNA from water samples to improve current surveillance *** methodology is highly sensitive with a detection limit of 5×10^(−6)ng/μL,detected in<20 minutes,with cumulative sample preparation and amplification time under 1 *** proposed workflow could assist in monitoring areas to determine the risk of Fascioliasis infection and implement strategies to manage snail populations to ultimately reduce the risk of infection for humans and livestock.

关键词： fasciola spp. Snail Molecular detection DNA diagnostics LAMP Environmental sampling eDNA

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