Development of a loop‑mediated isothermal amplification assay for detection of Austropeplea tomentosa from environmental water samples

作     者：Lily Tran Vignesh A.Rathinasamy Travis Beddoe 

作者机构：Department of AnimalPlant and Soil SciencesSchool of AgricultureBiomedicine and EnvironmentLa Trobe UniversityBundooraVIC 3083Australia Full list of author information is available at the end of the article 

出 版 物：《Animal Diseases》 (动物疾病（英文）)

年 卷 期：2023年第3卷第1期

页      面：35-48页

学科分类：0906[农学-兽医学] 09[农学] 

基　　金：supported by Cooperative Research Centres Project(CRCP)awarded to Geneworks and La Trobe University.L.T.is supported by an Australian Research Training Program scholarship and the Tim Healy Memorial Scholarship awarded by The Department of Primary Industries South Australia(PIRSA) 

主　　题：Fasciola spp. Snail Molecular detection DNA diagnostics LAMP Environmental sampling eDNA 

摘      要：Lymnaeid snails are key intermediate hosts for the development and survival of Fasciola spp.,the causative agent of Fascioliasis which are economically important parasites infecting humans and livestock *** current control method for treating Fascioliasis is heavily reliant on anthelmintic drugs,particularly Triclabendazole(TCBZ)which has resulted in drug-resistant parasites and poses significant risk as there are no long-term efficacious alternatives *** control measures at the farm level could include both parasite and snail control will play an important role in Fasciola *** and reduce the reliance on anthelmintic *** of such sustainable control measures requires effective identification of snails on the property however Lymnaeid snails are small and difficult to physically *** identification using an environmental DNA approach is a recent approach in which physically locating snails are not *** tomentosa,is the primary intermediate snail host for *** transmission in South-East Australia and we present an in-field loop-mediated isothermal amplification and water filtering method for the detection of *** eDNA from water samples to improve current surveillance *** methodology is highly sensitive with a detection limit of 5×10^(−6)ng/μL,detected in20 minutes,with cumulative sample preparation and amplification time under 1 *** proposed workflow could assist in monitoring areas to determine the risk of Fascioliasis infection and implement strategies to manage snail populations to ultimately reduce the risk of infection for humans and livestock.