检索结果-南通市图书馆

Recombinant Bivalent Vaccine against Foot-and-Mouth Disease Virus serotype o/A Infection in Guinea Pig

Acta Biochimica et Biophysica Sinica 2004年第9期36卷 589-596页

作者： Jian-Zhong YI1, Ming-Qiu LIU1, Cai-Zhu ZHU2, Qiang ZHANG1, Zu-Tian SHENG3, Qing-Yun DU3, Wei-Yao YAN1, and Zhao-Xin ZHENG1* State Key Laboratory of Genetics Engineering, Life Science School, Fudan University, Shanghai 200433, China 1The Veterinary Institute of Chinese Academy of Agricultural Sciences, Lanzhou 730046, China 2The Virus Research Institute of Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China The Veterinary Institute of Chinese Academy of Agricultural SciencesLanzhou 730046 China The Virus Research Institute of Zhejiang Academy of Agricultural SciencesHangzhou 310021 China State Key Laboratory of Genetics Engineering Life Science School Fudan UniversityShanghai 200433 China

In this study, two DNA fragments encoding amino acid (141–160)-(21–40)-(141–160) of the VP1 of fmdv (foot-and-mouth disease virus) serotype o and (138–160)-(21–40)-(138–160) of the serotype A fmdv were chemically synthesized. These two tandem-repeat fragments were ligated and transfected into prokaryotic expression vector pTrcHis A to construct pTH-o-A. The other vector called pTH-o-scIgG-A was constructed similarly only that the two tandem-repeat DNA fragments were linked by the bovine- IgG heavy chain coding sequence. Guinea pigs immunized with the two bivalent vaccines pTH-o-A and pTH-o-scIgG-A showed both specific antibody activity and T cell proliferation responses. fmdv challenge tests showed that 85% and 70% of guinea pigs vaccinated twice with 200 μg of the fusion protein of pTH-o-A were protected from fmdv serotype o and serotype A infection respectively. 70% and 57% of the guinea pigs immunized with the fusion protein of pTH-o-scIgG-A were protected from fmdv serotype o and serotype A infection respectively.

关键词： foot-and-mouth disease fmdv serotype o fmdv serotype A VP1 protein bivalent vaccine guinea pig

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：