Recombinant Bivalent Vaccine against Foot-and-Mouth Disease Virus Serotype O/A Infection in Guinea Pig

作     者：Jian-Zhong YI1, Ming-Qiu LIU1, Cai-Zhu ZHU2, Qiang ZHANG1, Zu-Tian SHENG3, Qing-Yun DU3, Wei-Yao YAN1, and Zhao-Xin ZHENG1* State Key Laboratory of Genetics Engineering, Life Science School, Fudan University, Shanghai 200433, China 1The Veterinary Institute of Chinese Academy of Agricultural Sciences, Lanzhou 730046, China 2The Virus Research Institute of Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China Jian-Zhong YI1, Ming-Qiu LIU1, Cai-Zhu ZHU2, Qiang ZHANG1, Zu-Tian SHENG3, Qing-Yun DU3, Wei-Yao YAN1, and Zhao-Xin ZHENG1* State Key Laboratory of Genetics Engineering, Life Science School, Fudan University, Shanghai 200433, China;1The Veterinary Institute of Chinese Academy of Agricultural Sciences, Lanzhou 730046, China;2The Virus Research Institute of Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

作者机构：The Veterinary Institute of Chinese Academy of Agricultural SciencesLanzhou 730046 China The Virus Research Institute of Zhejiang Academy of Agricultural SciencesHangzhou 310021 China State Key Laboratory of Genetics Engineering Life Science School Fudan UniversityShanghai 200433 China 

出 版 物：《Acta Biochimica et Biophysica Sinica》 (生物化学与生物物理学报(英文版))

年 卷 期：2004年第36卷第9期

页      面：589-596页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学] 0703[理学-化学] 

基　　金：This study was supported by a grant from the National High TechnologyResearch and Development Program of China (No. 2001AA213071) 

主　　题：foot-and-mouth disease FMDV serotype O FMDV serotype A VP1 protein bivalent vaccine guinea pig 

摘      要：In this study, two DNA fragments encoding amino acid (141–160)-(21–40)-(141–160) of the VP1 of FMDV (foot-and-mouth disease virus) serotype O and (138–160)-(21–40)-(138–160) of the serotype A FMDV were chemically synthesized. These two tandem-repeat fragments were ligated and transfected into prokaryotic expression vector pTrcHis A to construct pTH-O-A. The other vector called pTH-O-scIgG-A was constructed similarly only that the two tandem-repeat DNA fragments were linked by the bovine- IgG heavy chain coding sequence. Guinea pigs immunized with the two bivalent vaccines pTH-O-A and pTH-O-scIgG-A showed both specific antibody activity and T cell proliferation responses. FMDV challenge tests showed that 85% and 70% of guinea pigs vaccinated twice with 200 μg of the fusion protein of pTH-O-A were protected from FMDV serotype O and serotype A infection respectively. 70% and 57% of the guinea pigs immunized with the fusion protein of pTH-O-scIgG-A were protected from FMDV serotype O and serotype A infection respectively.