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fibroblast-localized lncRNA CFIRL promotes cardiac fibrosis and dysfunction in dilated cardiomyopathy

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Science China(Life Sciences) 2024年第6期67卷 1155-1169页

作者： Shuai Yuan Xudong Zhang Jiabing Zhan Rong Xie Jiahui Fan Beibei Dai Yanru Zhao Zhongwei Yin Qian Liu Dao Wen Wang Huaping Li Chen Chen Division of Cardiology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Hubei Key Laboratory of Genetics and Molecular Mechanisms of Cardiological Disorders Wuhan 430030China Department of Forensic Medicine Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

CFIRL is a long noncoding RNA(lnc RNA),we previously identified as the most significantly upregulated lnc RNA in the failing hearts of patients with dilated cardiomyopathy(DCM).In this study,we determined the function of CFIRL and its role in ***-time polymerase chain reaction and in situ hybridization assays revealed that CFIRL was primarily localized in the nucleus of cardiac fibroblasts and robustly increased in failing *** knockdown or fibroblast-specific knockout of CFIRL attenuated transverse aortic constriction(TAC)-induced cardiac dysfunction and fibrosis in *** of CFIRL in vitro promoted fibroblast proliferation and aggravated angiotensin II-induced differentiation to *** knockdown attenuated these ***,RNA pull-down assay and gene expression profiling revealed that CFIRL recruited ENO1,a newly identified noncanonical transcriptional factor,to activate ***-6 exerted a paracrine effect on cardiomyocytes to promote cardiac hypertrophy,which can be prevented by CFIRL *** findings uncover the critical role of CFIRL,a fibroblast-associated lnc RNA,in heart failure by facilitating crosstalk between fibroblasts and *** knockdown might be a potent strategy to prevent cardiac remodeling in heart failure,particularly in DCM.

关键词： lncRNA fibroblast fibrosis dilated cardiomyopathy heart failure

fibroblast growth factor receptor 4 protein expression and clinicopathological features in gastric cancer

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World Journal of Gastroenterology 2015年第6期21卷 1838-1844页

作者： Hao Chen Dan-Ping Shen Zi-Zhen Zhang Jia-Hua Liu Yan-Ying Shen Xing-Zhi Ni Department of General Surgery Ren Ji Hospital School of Medicine Shanghai Jiao Tong University Department of Pathology Ren Ji Hospital School of Medicine Shanghai Jiao Tong University

AIM:To investigate fibroblast growth factor receptor4(FGFR4)protein expression in Chinese patients with resectable gastric cancer(GC)and the association with clinicopathological characteristics and *** underwent curative surgical procedures were enrolled in this *** protein expression of FGFR4 in formalin-fixed,paraffin-embedded(FFPE)GC tissues was determined by immunohistochemical(IHC)*** clinicopathological data and survival information were also collected andχ2 statistical analysis was performed to analyze FGFR4 protein expression in the subgroups with differing clinicopathological characteristics including;gender,age,tumor location,differentiation,tumor-node-metastasis stage,macroscopic type,depth of invasion,lymph node metastases,distant metastasis,neural invasion and vascular ***,some common molecular markers of GC in our cancer center,including p53,p27,topoisomeraseⅡα(TopoⅡα)were also determined by IHC and their association with FGFR4 protein expression *** probability of survival for different subgroups with different clinicopathological characteristics was calculated using the Kaplan-Meier method and survival curves plotted using the log rank ***:Seventy seven cases(44%)were found to have high expression of FGFR4 *** different FGFR4 expression was observed between gastric cancers with differing expression of TopoⅡα(log rankχ2=9.4760,P=0.0236).No significant differences were observed between subgroups defined by any of the other clinicopathological *** median survival time of the FGFR4 high expression(77 cases)and low expression groups(98 cases)was27 mo and 39 mo,*** five-year survival rates and median survival times of gastric cancers with high FGFR4 expression were worse than those with low expression(30.8%vs 39.2%,27 mo vs 39 mo),respectively,however,no significant difference was observed in survival time(log rankχ2=1.0477,P=0.3060).Survival anal

关键词： Gastric cancer fibroblast growth factor receptor 4

A Comparative Study of fibroblast Behaviors under Cyclic Stress Stimulus and Static Culture on 3D Patterned Matrix

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Journal of Bionic Engineering 2013年第2期10卷 148-155页

作者： Mingyan Zhao Lihua Li Bo Li Di Wu Changren Zhou Ye Tian Department of Materials Science and Engineering Jinan University Guangzhou 510630 P. R. China Engineering Research Center of Artificial Organs and Materials (Ministry of Education China) Guangzhou 510630 P. R. China Guangdong Medical Devices and Test Institute Guangzhou 510632 P. R. China

Mechanical stress and patterned surface of the scaffolds has been recognized as a crucial factor in determining cell func- tionality and tissue development, which in turn can direct the cell responses. In this study, fibroblasts M-3T3 in three-dimensional （3D） honeycomb patterning Chitosan/Poly（L-Lactic Acid）（CS/PLLA） composites was stimulated by a 15% sinusoidal （1 Hz） strain applied by a biodynamic test instrument. The effects of mechanical stimulus on the cell proliferation and basic fibroblast Growth Factor （bFGF） secretion were studied in comparison to the non-strain groups and blank control. Results show that fibroblasts are able to sense the mechanical stimulation and respond, resulting in a time dependent increase of bFGF secretion and promoting cell proliferation. Moreover, the cells seeded in the scaffolds showed a higher cell proliferation and bFGF secretion. These findings support the hypothesis that suitable mechanical stimulus has positive effect on fibroblasts, and such a 3D honeycomb patterned scaffold may play a positive role in regulating cell behaviors in vitro.

关键词： polymer composites bFGF mechanical stimulus fibroblast

fibroblast growth factor receptor 4 Gly388Arg polymorphism in Chinese gastric cancer patients

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World Journal of Gastroenterology 2013年第28期19卷 4568-4575页

作者： Yan-Ying Shen Ya-Chao Lu Dan-Ping Shen Yuan-Jie Liu Xin-Ying Su Guan-Shan Zhu Xiao-Lu Yin Xing-Zhi Ni Department of Pathology Ren Ji Hospital School of Medicine Shanghai Jiao Tong University Innovation Center China Astra Zeneca Global RD Zhangjiang Hi-Tech Park Department of General Surgery Ren Ji Hospital School of Medicine Shanghai Jiao Tong University

AIM: To investigate the contribution of the fibroblast growth factor receptor 4 (FGFR4) Gly388Arg polymorphism as a genetic risk factor for gastric cancer (GC) and to investigate any associations between this polymorphism and clinicopathological parameters and survival. METHODS: Tumors and matched adjacent non-cancer tissues were collected from 304 GC patients, and 5 mL of venous blood was collected from 62 GC patients and 392 ageand sex-matched healthy controls without cancer history from the same ethnic population. DNA was extracted, and direct sequencing analyses were performed to genotype the FGFR4 Gly388Arg polymorphism in all the samples. Differences in the genotype frequencies of the FGFR4 Gly388Arg polymorphism between GC patients and healthy controls were estimated using the χ 2 test. Binary logistic regression was used for all analysis variables to estimate risk as the ORs with 95%CIs. The relationships between the FGFR4 genotype and clinicopathological parameters were tested with the χ 2 test. The Kaplan-Meier product-limit method, the log-rank test, and the Cox regression model were applied to evaluate the effect of the FGFR4 genotype on the overall survival of patients with GC. RESULTS: In the present GC cohort, 118 patients (38.8%) were homozygous for the Gly388 allele, 124 patients (40.8%) were heterozygous, and 62 patients (20.4%) were homozygous for the Arg388 allele. The frequencies of the Gly/Gly, Gly/Arg, and Arg/Arg genotypes in the healthy controls were 33.6%, 48.0%, and 18.4%, respectively. The distributions of genotypes (χ 2 = 3.589, P = 0.166) and alleles (χ 2 = 0.342, P = 0.559) of the FGFR4 Gly388Arg polymorphism were not different between the GC patients and the healthy controls. Although we observed no correlation between the FGFR4 Gly388Arg polymorphism and clinicopathological parameters or survival in the total cohort of GC patients, the presence of the Arg388 allele was associated with shorter survival time in patients with GC if the

关键词： fibroblast growth factor receptor 4 Gly388Arg Genetic susceptibility Single nucleotide polymorphism Gastric cancer

fibroblast growth factor receptor signaling as therapeutic targets in gastric cancer

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World Journal of Gastroenterology 2016年第8期22卷 2415-2423页

作者： Masakazu Yashiro Tasuku Matsuoka Department of Surgical Oncology Osaka City University Graduate School of Medicine Oncology Institute of Geriatrics and Medical Science Osaka City University Graduate School of Medicine

fibroblast growth factor receptors(FGFRs) regulate a variety of cellular functions, from embryogenesis to adult tissue homeostasis. FGFR signaling also plays significant roles in the proliferation, invasion, and survival of several types of tumor cells. FGFR-induced alterations, including gene amplification, chromosomal translocation, and mutations, have been shown to be associated with the tumor initiation and progression of gastric cancer, especially in diffuse-type cancers. Therefore, the FGFR signaling pathway might be one of the therapeutic targets in gastric cancer. This review aims to provide an overview of the role of FGFR signaling in tumorigenesis, tumor progression, proliferation, and chemoresistance. We also discuss the accumulating evidence that demonstrates the effectiveness of using clinical therapeutic agents to inhibit FGFR signaling for the treatment of gastric cancer.

关键词： fibroblast growth factor receptor Gastric cancer Signaling pathway Targeted therapy

Constructing Nanoscale Topology on the Surface of Microfibers Inhibits fibroblast Fibrosis

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Advanced Fiber Materials 2022年第5期4卷 1219-1232页

作者： Yongjie Jiao Xiaojing Li Jinjie Chen Chaojing Li Laijun Liu Xingxing Liu Fujun Wang Guang Chen Lu Wang Key Laboratory of Textile Science and Technology Ministry of EducationCollege of TextilesDonghua UniversityShanghai 201620China Healthcare Department of Taizhou Hospital Affiliated to Wenzhou Medical University(Zhejiang Province Taizhou Hospital) Taizhou 317000China Department of Vascular Surgery Taizhou Hospital Affiliated to Wenzhou Medical University(Zhejiang Province Taizhou Hospital)Taizhou 317000China

Fibrosis is a common problem in soft tissue regeneration,often caused by the differentiation of fibroblasts into *** of the nanoscale topology that regulates the mechanical transduction of cells,nanofibers or nanoparticles are commonly used to modulate fibroblast ***,the strength of nanofibers is insufficient,and the physi-ological toxicity of nanoparticles still remains to be *** this study,self-induced crystallization was used to construct nano-protrusions on the random and aligned polycaprolactone microfibers to regulate the behavior of *** results revealed that the mechanical properties of microfibers with a nanoscale topology were *** staining manifested that nano-protrusions impeded the activation of integrins and vinculins,thereby inhibiting the nuclear transfer of Yes-associated protein,resulting in a decrease in the expression ofα-smooth muscle *** topology of microfibers hampered the activation of the Rho/ROCK signalling *** general,we used a simple process to con-struct a fibrous scaffold with a micro-nano multilevel *** structure can hinder the transformation of fibroblasts into myofibroblasts on both random and aligned fibers,which is expected to prevent fibrosis.

关键词： Electrospinning Topology fibroblast Fibrosis

Prunella vulgaris Polysaccharide Inhibits Growth and Migration of Breast Carcinoma-Associated fibroblasts by Suppressing Expression of Basic fibroblast Growth Factor

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Chinese Journal of Integrative Medicine 2020年第4期26卷 270-276页

作者： HAO Jian DING Xiu-li YANG Xue WU Xiong-zhi Zhong-Shan-Men In-Patient Department Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjin(300060)China Clincal Center of Tumor Therapy The Fourth Central Hospital Affiliated to Nankai UniversityTianjin(300140)China

Objective:To study the effects of Prunella vulgaris polysaccharide(PVP)on human breast carcinoma-associated fibroblasts(CAFs).Method:Cell viability was detected by 3-[4,5-dimethylthiazol-2-yl]-2,5-(3-carboxymethoxyphenyl)-2-4-sulfophenyl)?2H?tetrazolium(MTS)*** healing experiment and transwell migration assay were used to investigate the anti-migration *** cytometry was applied to detect cell apoptosis and cell cycle *** transcription-polymerase chain reaction and enzyme-linked immunosorbent assay were used to detect the expression of basic fibroblast growth factor(bFGF)in *** SKBr-3 with CAFs conditioned medium(CAFs-CM)to evaluate the indirect function on the proliferation of breast cancer SKBr-3 ***:PVP inhibited the viability of CAFs by inducing apoptosis(P<0.01)and arresting cell cycle(P<0.01).It also inhibited the migration of CAFs(P<0.01).bFGF promoted CAFs proliferation(P<0.01)and migration(P<0.01),protected CAFs from apoptosis(P<0.05)and reduced Go phase to 49.06%(P<0.01).However,these effects of bFGF on CAFs could be abrogated by *** SKBr-3 with CAFs-CM,PVP could inhibit the viability of breast cancer SKBr-3 cells ***,PVP reduced the mRNA expression(P<0.01)and protein secretion of bFGF(P<0.01)in ***:PVP could exert an anti-cancer effect on breast CAFs by inhibiting bFGF expressi on,thus inhibit!ng the growth of breast can cer SKBr-3 cells in directly.

关键词： Prunella vulgaris polysaccharide carci no ma-associated fibroblasts basic fibroblast growth factor SKBr-3 Chinese medicine

Effect of mitomycin on normal dermal fibroblast and HaCat cell:an in vitro study

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第12期13卷 997-1005页

作者： Yao-wen WANG Ji-hao REN Kun XIA Shu-hui WANG Tuan-fang YIN Ding-hua XIE Li-hua LI Otolaryngology-Head and Neck Surgery Department Ningbo First HospitalNingbo 315000China Otolaryngology-Head and Neck Surgery Department the Seeond Xiangya HospitalCentral South UniversityChangsha 410001China State Key Laboratory of Medical Genetics Central South UniversityChangsha 410001China

Objective:To evaluate the effects of mitomycin on the growth of human dermal fibroblast and immortalized human keratinocyte line (HaCat cell),particularly the effect of mitomycin on intracellular messenger RNA (mRNA) synthesis of collagen and growth factors of ***:The normal dermal fibroblast and HaCat cell were cultured in *** cultures were exposed to 0.4 and 0.04 mg/ml of mitomycin solution,and serum-free culture medium was used as *** cellular morphology change,growth characteristics,cell proliferation,and apoptosis were observed at different *** the fibroblasts,the mRNA expression changes of transforming growth factor (TGF)-β1,basic fibroblast growth factor (bFGF),procollagen Ⅰ,and Ⅲ were detected by reverse transcription polymerase chain reaction (RT-PCR).Results:The cultured normal human skin fibroblast and HaCat cell grew exponentially.A 5-min exposure to mitomycin at either 0.4 or 0.04 mg/ml caused marked dose-dependent cell proliferation inhibition on both fibroblasts and HaCat *** morphology changed,cell density decreased,and the growth curves were without an exponential *** fibroblast proliferated on the 5th day after the 5-min exposure of mitomycin at 0.04 mg/***,5-min application of mitomycin at either 0.04 or 0.4 mg/ml induced fibroblast apoptosis but not *** apoptosis rate of the fibroblast increased with a higher concentration of mytomycin (pfibroblast proliferation,induce fibroblast apoptosis,and regulate intracellular protein expression on mRNA *** additon,mitomycin can inhibit HaCat cell proliferation,so epithelial cell needs more protecting to avoid mitomycin's side effect when it is applied clinically.

关键词： Mitomycin fibroblast HaCat cell Apoptosis TGF-β1 bFGF Procollagen

Cardiac fibroblast heat shock protein 47 aggravates cardiac fibrosis post myocardial ischemia-reperfusion injury by encouraging ubiquitin specific peptidase 10 dependent Smad4 deubiquitination

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Acta Pharmaceutica Sinica B 2022年第11期12卷 4138-4153页

作者： Saiyang Xie Yun Xing Wenke Shi Min Zhang Mengya Chen Wenxi Fang Shiqiang Liu Tong Zhang Xiaofeng Zeng Si Chen Shasha Wang Wei Deng Qizhu Tang Department of Cardiology Renmin Hospital of Wuhan UniversityWuhan 430060China Hubei Key Laboratory of Metabolic and Chronic Diseases Wuhan 430060China Cardiovascular Research Institute of Wuhan University Wthan 430060China

Despite complications were significantly reduced due to the popularity of percutaneous coronary intervention(PCI) in clinical trials, reperfusion injury and chronic cardiac remodeling significantly contribute to poor prognosis and rehabilitation in AMI patients. We revealed the effects of HSP47 on myocardial ischemia-reperfusion injury(IRI) and shed light on the underlying molecular *** generated adult mice with lentivirus-mediated or miRNA(mi1/133TS)-aided cardiac fibroblastselective HSP47 overexpression. Myocardial IRI was induced by 45-min occlusion of the left anterior descending(LAD) artery followed by 24 h reperfusion in mice, while ischemia-mediated cardiac remodeling was induced by four weeks of reperfusion. Also, the role of HSP47 in fibrogenesis was evaluated in cardiac fibroblasts following hypoxia-reoxygenation(HR). Extensive HSP47 was observed in murine infarcted hearts, human ischemic hearts, and cardiac fibroblasts and accelerated oxidative stress and apoptosis after myocardial IRI. Cardiac fibroblast-selective HSP47 overexpression exacerbated cardiac dysfunction caused by chronic myocardial IRI and presented deteriorative fibrosis and cell ***47 upregulation in cardiac fibroblasts promoted TGFβ1-Smad4 pathway activation and Smad4 deubiquitination by recruiting ubiquitin-specific peptidase 10(USP10) in fibroblasts. However, cardiac fibroblast specific USP10 deficiency abolished HSP47-mediated fibrogenesis in hearts. Moreover, blockage of HSP47 with Col003 disturbed fibrogenesis in fibroblasts following HR. Altogether, cardiac fibroblast HSP47 aggravates fibrosis post-myocardial IRI by enhancing USP10-dependent Smad4 deubiquitination,which provided a potential strategy for myocardial IRI and cardiac remodeling.

关键词： Heat shock protein 47 Myocardial ischemia-reperfusion injury Ubiquitin-specific protease 10 Cardiac fibrosis s Smad4 fibroblast Cell proliferation Cardiae dysfunction