检索结果-南通市图书馆

evans blue staining to detect deep blood vessels in peripheral retina for observing retinal pathology in early-stage diabetic rats

引用

International Journal of Ophthalmology(English edition) 2021年第10期14卷 1501-1507页

作者： Kang-Pei Shi Yun-Tong Li Chuang-Xin Huang Chu-Sheng Cai Yan-Jie Zhu Lei Wang Xiao-Bo Zhu State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangzhou 510060Guangdong ProvinceChina

AIM: To observe and compare the statistical significance of superficial and deep vascular leakage in the pathological changes of the diabetic rats retina after the evans blue(EB) perfusion, and utilize the modified whole-retina spreading method to make the slides while protecting the periphery of the retina. METHODS: The Sprague-Dawley(SD) rats were randomly divided into 6 groups. Each group named as the normal groups for 4, 8, and 12 wk and the diabetic groups for 4, 8, and 12 wk. The EB was injected into the cardiovascular system of the rats at the different time points. The retina of each group was obtained for ***: The superficial vascular leakage was found in all 6 groups. The size of leakage area of superficial retinal blood vessels was(0.54±0.23)%,(0.65±0.11)%,and(0.58±0.10)% in normal group. No notable leakage was found in the deep blood vessels [(0.03±0.04)%,(0.03±0.05)%, and(0.03±0.05)%]. The deep retinal vascular leakage was found in the peripheral retina of diabetic rats. The size of leakage area of superficial retinal blood vessels in diabetic group were(0.53±0.22)%,(0.69±0.16)%, and(0.52±0.11)%. The leakage areas of deep blood vessels were(0.54±0.50)%,(1.42±0.16)%, and(1.80±0.07)% at 4, 8, and 12 wk, respectively. There was a statistically difference of the leakage area between the 8 th week and the 4 th week of diabetes group(P=0.003). The statistically significant difference between the diabetes and the control groups was noted at 4 wk and 8 wk(P<0.001).CONCLUSION: The main retinal pathological changes of early-stage diabetic rats are the vascular leakage of the periphery of deep retina. Diabetic rats modeled after 8 wk have semi-quantitative statistical difference compared with the normal rats, thus early intervention treatment research can start at this time point.

关键词： evans blue whole-mounted rat retina preparation diabetic retinopathy diabetic blood retinal barrier Sprague-Dawley rats

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Resonance Rayleigh Scattering Method for the Determination of Raloxifene with evans blue

引用

Chinese Journal of Chemistry 2002年第12期20卷 1552-1556,1462页

作者：范莉刘绍璞杨大成胡小莉 Institute of Environmental Chemistry Southwest China Normal University Chongqing 400715 China Email: Shao-Pu Liu (liusp@***)

The intensity of resonance Rayleigh scattering (RRS) of evans blue (EB) or raloxifene hydrochloride (Ralo) is very weak, but it can be enhanced significantly and a new RRS spectrum appears when both of them interact to form an ion-association complex in sodium acetate-hydrochloric acid buffer solution at pH 1.8. The intensity of RRS is directly proportional to the concentration of Ralo in the range of 0–8.3 μg · mL?1, and the detection limit for Ralo (σ = 3) is 18.9 ng·mL?1. The method has high sensitivity and fairly good selectivity. Based on the above evidences, a new facile method for the determination of trace amount of Ralo has been established with satisfactory results.

关键词： resonance Rayleigh scattering raloxifene evans blue ion-association complex

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Comparative Efficacy and Distribution of evans blue Liposome Modified with DSPE-PEG,Tween80,and Brij35

引用

Journal of Chinese Pharmaceutical Sciences 2003年第2期12卷 71-75页

作者：张小滨侯新朴北京大学药学院物理药学室北京100083

Aim To improve the stability and optimize the tissue distribution of evans blue liposome in rats, some surfactants such as DSPE-PEG, Tween80, and Brij35 were used to modify the evans blue liposome. Methods The evans blue liposome was prepared by the reverse-phase-evaporation method. The effect of cholesterol on the encapsulation percentage of evans blue was studied. The effects of DSPE-PEG, Tween80, and Brij35 on the encapsulation percentage and tissue distribution of evans blue liposome in the rat were determined. Results The top encapsulation percentage of evans blue liposome is 25.30%. After modification by DSPE-PEG, Tween80, and Brij35, the encapsulation percentages decreased slightly, but not significantly. After modification, the evans blue concentrations deceased in the liver, spleen, lung and kidney, but increased in the brain, especially in the EB-L-Tween80 group. Conclusion DSPE-PEG, Tween80, and Brij35 have slight effect on the encapsulation percentage of evans blue liposome. The effect of Brij35 on the distribution of evans blue liposome is similar to that of DSPE-PEG because they both is prevent the reticuloendothelial system (RES) from clearing liposome. The evans blue concentration in the brain is greatly improved when Tween80 is used to modify the EB liposome, which is good information for preparing liposome targeting the brain.

关键词： evans blue liposomes DSPE-PEG Tween80 Brij35 tissue distribution

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

基于聚类分析及因子分析对急性肠粘膜损伤大鼠背部敏化区域evans blue渗出强度及规律的分析

基于聚类分析及因子分析对急性肠粘膜损伤大鼠背部敏化区域Evans ...

引用

新时代新思维新跨越新发展——2019中国针灸学会年会暨40周年回顾

作者：林燊林栋王芳玲洪霖齐诗仪莫毓昌福建中医药大学针灸学院华侨大学数学科学学院

目的:观察急性肠粘膜损伤相关的体表evans blue渗出点分布情况。方法:SD雄性大鼠30只,按芥子油浓度随机分为5组,分别为5%、7.5%、10%、12.5%、15%,每组6只。采用肠道注射芥子油造成急性肠粘膜损伤模型,造模后4小时背部备皮、尾静脉注射... 详细信息

目的:观察急性肠粘膜损伤相关的体表evans blue渗出点分布情况。方法:SD雄性大鼠30只,按芥子油浓度随机分为5组,分别为5%、7.5%、10%、12.5%、15%,每组6只。采用肠道注射芥子油造成急性肠粘膜损伤模型,造模后4小时背部备皮、尾静脉注射EB,分别观察造模后5、10、15、20、25小时EB渗出点的分布。采用经纬网格计数结合多元统计分析的方法,分析EB渗出点随浓度及时间变化的分布规律。结果:造模后25 h内各组大鼠均存活。(1)聚类分析渗出点在S1、N1、O14、L11网格分布特异性突出,轮廓分析表明四个"特征网格"随浓度与时间变化中无明显差异(P>0.05)。(2)因子分析渗出"特征区域"在距正中线旁开约0～10 mm或15～20 mm范围内分布较多。轮廓分析结果,在各时间点随浓度变化的EB渗出"特征区域"有显著性差异(P0.05)。结论:急性肠黏膜损伤大鼠EB渗出网格点分布规律主要在胸腰段的脊柱两侧,特征区域分布与大鼠肺、脾俞及肾俞相近,并有与病情变化相关的规律性。

关键词：针灸推拿学敏化穴位因子分析急性肠粘膜损伤 evans blue

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Vascular endothelial growth factor-165b protects the blood-retinal barrier from damage after acute high intraocular pressure in rats

引用

International Journal of Ophthalmology(English edition) 2022年第8期15卷 1231-1239页

作者： Jing Shen Yi Li Min Li Wei-Xian Liu Hong-Liang Sun Quan-Peng Zhang Xi-Nan Yi Department of Ophthalmology First Affiliated Hospital of Hainan Medical UniversityHaikou 570102Hainan ProvinceChina Grade 18 undergraduate of First Clinical College Hainan Medical UniversityHaikou 571199Hainan ProvinceChina Department of Human Anatomy and Neurobiology School of Basic Medical SciencesCentral South UniversityChangsha 410013Hunan ProvinceChina Key Laboratory of Brain Science Research&Transformation in Tropical Environment of Hainan Province Hainan Medical UniversityHaikou 571199Hainan ProvinceChina Anatomy Laboratory of Hainan Medical University Haikou 571199Hainan ProvinceChina

AIM:To elucidate the role of vascular endothelial growth factor-165b(VEGF-165b)in blood-retinal barrier(BRB)injury in the rat acute glaucoma ***:In this study,the rat acute high intraocular pressure(HIOP)model was established before and after intravitreous injection of anti-VEGF-165b *** expression of VEGF-165b and zonula occludens-1(ZO-1)in rat retina was detected by double immunofluorescence staining and Western blotting,and the breakdown of BRB was detected by evans blue(EB)***:The intact retina of rats expressed VEGF-165b and ZO-1 protein,which were mainly located in the retinal ganglion cell layer and the inner nuclear layer and were both co-expressed with vascular endothelial cell markers *** acute HIOP,the expression of VEGF-165b was up-regulated;the expression of ZO-1 was down-regulated at 12h and then recovered at 3d;EB leakage increased,peaking at *** intravitreous injection of anti-VEGF-165b antibody,the expression of VEGF-165b protein was no significantly changed;and the down-regulation of the expression of ZO-1 was more obvious;EB leakage became more serious,peaking at *** analysis also showed that EB leakage in the peripheral retina was greater than that in the central ***:The endogenous VEGF-165b protein may protect the BRB from acute HIOP by regulating the expression of *** differential destruction of BRB after acute HIOP may be related to the selective loss of retinal ganglion cells.

关键词： vascular endothelial growth factor-165b blood-retinal barrier high intraocular pressure evans blue zonula occludens-1

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Correlation of aquaporin-4 expression to blood-brain barrier permeability in rats with focal cerebral ischemia

引用

Neural Regeneration Research 2008年第6期3卷 629-633页

作者： Pengcheng Xu Haorong Feng Jinbu Xu Yongping Wu Jiangsu Province Key Laboratory of Anesthesiology Xuzhou Medical College Xuzhou 221002 Jiangsu Province China Department of Anesthesiology the 98 Hospital of Chinese PLA Huzhou 313000 Zhejiang Province China

BACKGROUND： Ischemic cerebrovascular disease causes injury to the blood-brain barrier. The occurrence of brain edema is associated with aquaporin expression following cerebral ischemia/reperfusion. OBJECTIVE： To analyze the correlation of aquaporin-4 expression to brain edema and blood-brain barrier permeability in brain tissues of rat models of ischemia/reperfusion. DESIGN, TIME AND SETTING： The randomized control experiment was performed at the Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College, China from December 2006 to October 2007. MATERIALS： A total of 112 adult, male, Sprague-Dawley rats, weighing 220-250 g, were used to establish rat models of middle cerebral artery occlusion and reperfusion by the suture method. Rabbit anti-aquaporin-4 （Santa Cruz, USA） and evans blue （Sigma, USA） were used to analyze the tissue. METHODS： The rats were randomized into sham-operated （n = 16） and ischemia/reperfusion （n = 96） groups. There were 6 time points in the ischemia/reperfusion group, comprising 4, 6, 12, 24, 48, and 72 hours after reperfusion, with 16 rats for each time point. Rat models in the sham-operated group at 4 hours after surgery and rat models in the ischemia/reperfusion group at different time points were equally and randomly assigned into 4 different subgroups. MAIN OUTCOME MEASURES： Brain water content on the ischemic side and the control side was measured using the dry-wet weight method. Blood-brain barrier function was determined by evans blue. Aquaporin-4 expression surrounding the ischemic focus, as well as the correlation of aquaporin-4 expression with brain water content and evans blue staining, were measured using immunohistochemistry and Western blot analysis. RESULTS： Brain water content on the ischemic side significantly increased at 12 hours after reperfusion, reached a peak at 48 hours, and was still high at 72 hours. Brain water content was greater on the ischemic hemispheres, compared with the control hemispheres at

关键词： aquaporin-4 blood-brain barrier evans blue brain edema

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of Electromagnetic Pulse Exposure on Permeability of Blood-testicle Barrier in Mice

引用

Biomedical and Environmental Sciences 2008年第3期21卷 218-221页

作者： XIAO-WU WANG GUI-RONG DING CHANG-HONG SHI TAO ZHAO JIE ZHANG LI-HUA ZENG Guo-ZHEN GUO Department of Radiation Medicine Faculty of Preventive Medicine Fourth Military Medical University Laboratory Animal Research Center Fourth Military Medical University Xi＇an 710032 Shaanxi China

Objective To study the effect of electromagnetic pulse （EMP） exposure on the permeability of blood-testicle barrier （BTB） in mice. Methods Adult male BALB/c mice were exposed to EMP at 200 kV/m for 200 pulses with 2 seconds interval. The mice were injected with 2% evans blue solution through caudal vein at different time points after exposure, and the permeability of BTB was monitored using a fluorescence microscope. The testis sample for the transmission electron microscopy was prepared at 2 h after EMP exposure. The permeability of BTB in mice was observed by using evans blue tracer and lanthanum nitrate tracer. Results After exposure, cloudy evans blue was found in the testicle convoluted seminiferous tubule of mice. Lanthanum nitrate was observed not only between testicle spermatogonia near seminiferous tubule wall and sertoli cells, but also between sertoli cells and primary spermatocyte or secondary spermatocyte. In contrast, lanthanum nitrate in control group was only found in the testicle sertoli cells between seminiferous tubule and near seminifdrous tubule wall. Conclusion EMP exposure could increase the permeability of BTB in the mice.

关键词： Electromagnetic pulse (EMP) Blood-testicle barrier (BTB) Lanthanum evans blue Permeability Mice

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of electromagnetic pulse exposure on the permeability of blood-testicle barrier in mice

Effect of electromagnetic pulse exposure on the permeability...

引用

The Fourth Asia-Pacific Conference on Environmental Electromagnetics

作者： Wang Xiao-Wu Guo Guo-Zhen Ding Gui-Rong Zhao Tao Zhang Jie Zeng Li-Hua Department of Radiation Medicine Faculty of Preventive Medicine Fourth Military Medical University Xi’an 710032 China

＜正＞AIM To study the effect of electromagnetic pulse （EMP） exposure on the permeability of Blood-testicle barrier（BTB） in mice. METHODS Adult male BALB/c mice were exposed to EMP at 200kV/m for 200 pulses with 2 seconds, interval. The mice were injected with 2% evans blue solution through caudal vein at different time point after exposure, and the permeability -of BTB was observed by fluorescence microscope. The testis sample for the transmission electron microscopy was prepared at 2h after EMP exposure. The permeability of BTB in mice was observed by using evans blue tracer and lanthanum nitrate tracer. RESULTS After- exposure, cloudy evans blue was found in the testicle convoluted seminiferous tubule of mice. Lanthanum nitrate was observed not only between testicle spermatogonia near seminiferous tubule wall and sertoli cells, but also between sertoli cells and primary spermatocyte or secondary spermatocyte. However, in control group lanthanum, nitrate was only found in the testicle sertoli cells between seminiferous tubule and near seminiferous tubule wall. CONCLUSION EMP exposure could increase the permeability of BTB in the mice.

关键词： Electromagnetic pulse (EMP) Blood-testicle barrier(BTB) Lanthanum evans blue Permeability Mice

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Spared Nerve Injury alters the blood-spinal cord barrier permeability:light-,electro-microscopy

Spared Nerve Injury alters the blood-spinal cord barrier per...

引用

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Li DUAN~1,Yan XU~2,Ying-Fei XIONG~1,Rong CAO~1,Ling-Feng TU~1,Zhi-Ren RAO~(1,*) 1 Institute of Neurosciences Fourth Military Medical University,Xi''an,710032. 2 Departement of Neurology,Xijing Hospital.Fourth Military Medical University Xi''an 710032

＜正＞Objective To investigate the change of blood-spinal cord barrier permeability after the Spared Nerve Injury （SNI） in adult ***（1） Male SD rats were divided into 3 groups randomly（control,sham-SNI group and SNI group） .The rats were performed SNI and survived for 3d,10d and 20d *** blue was injected into caudal vein,after 30 min,the rats were perfused and spinal cord were removed,L4 segment of spinal cord were cut into 30μm frontal sections on a *** section was detected under Olympus BX-51 fluorescent microscope.（2） ImmunoelectromocroOscopic DAB staining of IgG was performed at 10d,20d after SNI and detected under electron ***（1） Red fluorescent staining-blood vessel and effusion could not be found in the spinal cord dorsal horn of control *** 3d group,weak red fluorescent staining-blood vessel was *** 10d group,obvious red fluorescent staining-blood vessel was detected with few *** 20d group,the dorsal horn exhibited wide enlarge capillaries with obvious red- staining vessel wall and many clear effusion（P＞0.05）.（2） At SNI 10d group,weak electron density IgG was detected in the capillary endothelium,but at SNI 20d group,electron density IgG was higher,the granule and flocculent *** three type ultrastructures for IgG exudation from the capillary were found using electron microscopy,（a） The first means was IgG from endothelium,through pericytes and basement membrane exudates to perivascular space,（b） The second means was adsorptive-mediated transcytosis（AMT） processes,the endothelial performs AMT through "endocytosis" and transport and / or "exocytosis" steps.（c）The third means was through the tight junctions open of endothelium. Conclusion BSCB is not unusual open after SNI 3d;at SNI 10d,BSCB is mild opened unusual;at SNI 20d,the opened BSCB is peak,local BSCB permeability increased and subsequently increase the activity of neurons and *** open window can provide effective ther

关键词： blood-spinal cord barrier evans blue SNI endothelial cell rat

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

大鼠脊髓损伤后血—脊髓屏障的变化及其与继发性损伤关系的研究

大鼠脊髓损伤后血—脊髓屏障的变化及其与继发性损伤关系的研究

引用

作者：毛星刚第四军医大学

学位级别：硕士

脊髓损伤(spinal cord injury, SCI)往往造成患者的终生瘫痪,给家庭和社会带来沉重负担。因而,对SCI后病理变化机制及其相关研究一直是学者们所关注的热点与难点。SCI后血管和神经组织的机械性损坏和断裂引起原发性损伤,继而引发出血、... 详细信息

脊髓损伤(spinal cord injury, SCI)往往造成患者的终生瘫痪,给家庭和社会带来沉重负担。因而,对SCI后病理变化机制及其相关研究一直是学者们所关注的热点与难点。SCI后血管和神经组织的机械性损坏和断裂引起原发性损伤,继而引发出血、炎症、兴奋性毒性等一系列复杂的损伤过程,导致继发性损伤的进一步扩大。其中原发性损伤中血管的损害可直接引起血-脊髓屏障(blood spinal cord barrier,BSCB)的破坏,而BSCB的破坏导致血液中的各种分子进入脊髓实质,造成脊髓组织水肿、缺血缺氧、炎症以及脊髓血液灌注的改变,进一步加重脊髓的损伤。由于BSCB在脊髓损伤过程中的重要性,人们对其进行了大量的研究,但是脊髓损伤后BSCB的变化及其与继发性损伤的关系目前尚未完全清楚。为此,我们以大鼠脊髓撞击伤为模型,研究了SCI后BSCB的变化以及其与继发性损伤的关系。本实验分为两部分。第一部分研究了用免疫球蛋白G(IgG)免疫组织化学染色研究SCI后BSCB的变化。在以往的实验中一般采用示踪剂注射的方法研究SCI后BSCB的通透性变化,但此方法操作复杂,并且会影响其他形态的观察。为了寻找简洁可靠的方法,本实验采用IgG免疫组织化学方法染色,显示BSCB对内源性IgG的通透性。evans blue(EB)是研究BSCB的一种常用示踪剂,我们比较了SCI后0 h、8h、24h、72h、1w各个时间点的IgG染色范围和EB分布范围。结果表明,在各个时间点,IgG的染色范围(mm)为:7.14±0.50、11.43±1.63、9.48±1.71、9.82±0.63、5.59±0.55,EB的分布范围为:7.2±0.51、11.25±1.60、9.27±1.65、9.42±0.54、5.58±0.48,二者在头尾方向上的分布几乎重合。因而IgG染色可作为评估BSCB通透性的一项方便可靠的方法。第二部分,我们采用上述IgG免疫组织化学染色评估SCI后BSCB的变化。对于组织继发性损伤的发展,我们采用HE染色评估组织受损的范围,采用NeuN免疫组织化学染色评估神经元坏死的范围。并比较了IgG染色范围与上述组织损伤范围的变化。在损伤后0 h、4 h、8 h、24 h、72 h、1 w各个时间点,组织的损伤范围(mm)分别为3.64±0.92、6.03±0.47、7.04±0.42、7.11±0.53、9.32±0.17、8.63±0.31,IgG阳性范围分别为7.27±0.43、7.74±0.53、11.43±1.60、9.48±1.65、9.90±0.60、5.55±0.40。IgG阳性区域事实上包括了BSCB破坏的范围和IgG扩散的范围。在0 h-72 h之间,继发性损伤范围不断增加,IgG范围也呈现不断增加的趋势,其中在8 h时达到一个高峰。在72 h到1 w之间,组织损伤范围变化不明显,而IgG的范围则明显减小(p0.05)。故NYU撞击伤后,神经元的坏死主要发生于24 h之内,早期的神经元坏死可能是由于机械性的损伤所致。综上所述,利用IgG染色指示BSCB对内源性IgG的通透性,具有较多优势,可作为评估NYU脊髓损伤后BSCB通透性的一个方便可靠的指标。SCI后BSCB破坏的范围在72 h之内随着继发性损伤范围的增加逐渐增加,之后损伤范围逐渐稳定,BSCB破坏的范围也逐渐减小。NYU撞击伤后神经元的坏死主要为机械性损伤,且主要发生于24 h内。在SCI后神经元内出现IgG的原因和意义尚待进一步研究。

关键词：脊髓损伤血脊髓屏障白蛋白免疫球蛋白 evans blue 大鼠

来源：

同方学位论文库评论

在线全文

同方学位论文库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：