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Cell-type specificity of β-actin expression and its clinicopathological correlation in gastric adenocarcinoma

World Journal of Gastroenterology 2014年第34期20卷 12202-12211页

作者： Shafqat A Khan Monica Tyagi Ajit K Sharma Savio G Barreto Bhawna Sirohi Mukta Ramadwar Shailesh V Shrikhande Sanjay Gupta Epigenetics and Chromatin Biology Group Cancer Research InstituteAdvanced Centre for Treatment Research and Education in CancerTata Memorial CentreKhargharNavi MumbaiMH 410210India Gastrointestinal and Hepato-Pancreato-Biliary Service Department of Surgical OncologyTata Memorial HospitalTata Memorial CentreMumbaiMH 400012India Medanta Institute of Hepatobiliary and Digestive Sciences MedantaThe MedicityGurgaonHaryana 122001India Medical Oncology-GI and Breast Unit Tata Memorial HospitalTata Memorial CentreMumbaiMH 400012India Department of Pathology Tata Memorial HospitalTata Memorial CentreMumbaiMH 400012India

AIM:To investigate cell type specific distribution ofβ-actin expression in gastric adenocarcinoma and its correlation with clinicopathological parameters.METHODS:β-actin is a housekeeping gene,frequently used as loading control,but,differentially expresses in cancer.In gastric cancer,an overall increased expression ofβ-actin has been reported using tissue disruptive techniques.At present,no histological data is available to indicate its cell type-specific expression and distribution pattern.In the present study,we analyzedβ-actin expression and distribution in paired normal and tumor tissue samples of gastric adenocarcinoma patients using immunohistochemistry(IHC),a tissue non-disruptive technique as well as tissue disruptive techniques like reverse transcriptase-polymerase chain reaction(RT-PCR)and western blotting.Correlation ofβ-actin level with clinicopathological parameters was done using univariate analysis.RESULTS:The results of this study showed significant overexpression,at both mRNA and protein level in tumor tissues as confirmed by RT-PCR(1.47±0.13 vs2.36±0.16;Pepithelial and inflammatory cells of the tissues.Inflammatory cells showed a significantly higher expression compared to epithelial cells in normal(2.46±0.13 vs 5.92±0.23,Pepithelial and inflammatory cells overexpressβ-actin in tumor tissues,however,significant difference was observed only in inflammatory cells(5.92±0.23vs 6.71±0.14,Pepithelial and inflammatory cells also showed significant increase(4.19±0.15 vs 4.75±0.14,Pepithelial cells exhibited negative correlation(P>0.05).CO

关键词： Gastric cancer β-actin Immunohistochemistry epithe

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Giant gastrointestinal stromal tumour of rare sarcomatoid epithelioid subtype:Case study and literature review

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World Journal of Gastroenterology 2015年第11期21卷 3388-3393页

作者： Gustaw Lech Wojciech Korcz Emilia Kowalczyk Tomasz Guzel Marcin Radoch Ireneusz Wojciech Krasnodebski Department of General Gastroenterological and Oncological SurgeryMedical University of Warsaw Students’ Scientific Group at Department of General Gastroenterological and Oncological SurgeryMedical University of Warsaw

Gastrointestinal stromal tumours(GISTs) are the most common mesenchymal tumours of the gastrointestinal tract,but they represent less than 3% of all gastrointestinal tract malignancies.This is a detailed case study of a 52-yearold male patient treated for very uncommon histological subtype of gastric GIST with atypical clinical presentation,asymptomatic progress and late diagnosis.The resected tumour,giant in diameters,was confirmed to represent the most rare histopathologic subtype of GISTs- sarcomatoid epithelioid GIST.We report this case and review the literature with a special focus on pathomorphological evaluation,biological aggressiveness and prognostic factors.To our knowledge this is the first report of giant GIST of very uncommon sarcomatoid epithelioid subtype.It is concluded that clinicians should pay attention to the fact that initial diagnosis may be delayed due to mildly asymptomatic and non-specific clinical presentation.Asymptomatic tumours diagnosed at a late stage,which is often the case,can be large on presentation.Prognosis for patients diagnosed with GIST depend on tumour size,mitotic rate,histopathologic subtype and tumour location.That is why early diagnosis and R0 resection,which is usually feasible and safe even in giant gastric sarcomatoid epithelioid subtype of GISTs,are the key factors for further treatment and good prognosis.

关键词： Gastrointestinal stromal tumour Sarcomatoid epithe

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