Cell-type specificity of β-actin expression and its clinicopathological correlation in gastric adenocarcinoma

作     者：Shafqat A Khan Monica Tyagi Ajit K Sharma Savio G Barreto Bhawna Sirohi Mukta Ramadwar Shailesh V Shrikhande Sanjay Gupta 

作者机构：Epigenetics and Chromatin Biology GroupCancer Research InstituteAdvanced Centre for Treatment Research and Education in CancerTata Memorial CentreKhargharNavi MumbaiMH 410210India Gastrointestinal and Hepato-Pancreato-Biliary ServiceDepartment of Surgical OncologyTata Memorial HospitalTata Memorial CentreMumbaiMH 400012India Medanta Institute of Hepatobiliary and Digestive SciencesMedantaThe MedicityGurgaonHaryana 122001India Medical Oncology-GI and Breast UnitTata Memorial HospitalTata Memorial CentreMumbaiMH 400012India Department of PathologyTata Memorial HospitalTata Memorial CentreMumbaiMH 400012India 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2014年第20卷第34期

页      面：12202-12211页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Supported by TMH-IRG for project funding(account number-466) Advanced Center for Treatment Research and Education in Cancer India for funding to Gupta lab 

主　　题：Gastric cancer β-actin Immunohistochemistry Epithe 

摘      要：AIM:To investigate cell type specific distribution ofβ-actin expression in gastric adenocarcinoma and its correlation with clinicopathological parameters.METHODS:β-actin is a housekeeping gene,frequently used as loading control,but,differentially expresses in cancer.In gastric cancer,an overall increased expression ofβ-actin has been reported using tissue disruptive techniques.At present,no histological data is available to indicate its cell type-specific expression and distribution pattern.In the present study,we analyzedβ-actin expression and distribution in paired normal and tumor tissue samples of gastric adenocarcinoma patients using immunohistochemistry(IHC),a tissue non-disruptive technique as well as tissue disruptive techniques like reverse transcriptase-polymerase chain reaction(RT-PCR)and western blotting.Correlation ofβ-actin level with clinicopathological parameters was done using univariate analysis.RESULTS:The results of this study showed significant overexpression,at both mRNA and protein level in tumor tissues as confirmed by RT-PCR(1.47±0.13 vs2.36±0.16;P0.05).CONCLUSION:In gastric cancer,β-actin showed an overall higher expression predominantly contributed by inflammatory or tumor infiltrating immune cells of the tissue microenvironment and correlates with tumor grade.